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PR00641

Identifier
CHEMOKINER7  [View Relations]  [View Alignment]  
Accession
PR00641
No. of Motifs
9
Creation Date
20-MAR-1999
Title
C-C chemokine receptor type 7 signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR00657 CCCHEMOKINER
INTERPRO; IPR001718
GCRDB; GCR_0492; GCR_0958
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Chemokines.
In THE G-PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, pp83-88.
 
7. BIRKENBACH, M., JOSEFSEN, K., YALAMANCHILI, R., LENOIR, G. AND KIEFF, E. 
Epstein-Barr virus-induced genes: first lymphocyte-specific G protein-
coupled peptide receptors.
J.VIROL. 67 2209-20 (1993).
 
8. SCHWEICKART, V.L., RAPORT, C.J., GODISKA, R., BYERS, M.G., EDDY, R.L.,
SHOWS, T.B. AND GRAY, P.W.
Cloning of human and mouse EBI1, a lymphoid-specific G protein-coupled 
receptor encoded on human chromosome 17q12-q21.2.
GENOMICS 23 643-650 (1994). 

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Chemokines are proteins that have important physiological and patho- 
physiological roles in a wide range of acute and chronic inflammatory
processes [6]. Their sequences are similar and are characterised by a 
4-cysteine motif: the family can be divided according to whether the
first 2 Cys residues are adjacent (the C-C family), or separated by an
intervening residue (the C-x-C family). C-C chemokines are chemoattractant
for monocytes but not for neutrophils. The C-C family includes human
monocyte chemotactic protein-1 (MCP-1), regulated on activation, normal
T cell expressed and secreted (RANTES) and macrophage inflammatory 
proteins (MIP-1a and MIP-1b) [6].
 
C-C chemokine receptors are found in monocytes, lymphocytes, basophils and
eosinophils; mRNA is also found in some cell lines [6]. MCP-1 and MIP-1a
induce activation in low nanomolar concentrations and are highly selective
relative to C-x-C receptors. Calcium mobilisation has been demonstrated in
monocytes and in cells expressing the recombinant C-C receptor via an
uncharacterised G protein; pertussis toxin inhibits some of its actions [6].
 
C-C CKR-7 (formerly designated Epstein-Barr virus induced, EBI-1, receptor)
is expressed exclusively in B- and T-lymphocyte cell lines and in lymphoid 
tissues [7]. The receptor is likely to be a mediator of EBV effects on B 
lymphocytes, or of normal lymphocyte functions [7]. C-C CKR-7 is unique in
that both of its introns interrupt the coding region of the first extra-
cellular domain [8]. The gene is encoded on human chromosome 17q12-q21.2 [8].
Murine C-C CKR-7 cDNA has also been isolated and encodes a protein with 86%
identity to the human homologue. 
 
CHEMOKINER7 is a 9-element fingerprint that provides a signature for type 7
C-C chemokine receptors. The fingerprint was derived from an initial 
alignment of 2 sequences: the motifs were drawn from conserved regions 
spanning virtually the full alignment length, focusing on those sections
that characterise the type 7 receptors but distinguish them from the rest of
the C-C chemokine receptor family - motifs 1 and 2 reside at the N-terminus;
motif 3 lies in the second cytoplasmic loop; motifs 4 and 5 span the second
external loop; motifs 6 and 7 span the third external loop and part of TM
domain 7; and motifs 8 and 9 reside at the C-terminus. A single iteration 
on SPTR37_9f was required to reach convergence, no further sequences being 
identified beyond the starting set.
Summary Information
2 codes involving  9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
9222222222
8000000000
7000000000
6000000000
5000000000
4000000000
3000000000
2000000000
123456789
True Positives
CKR7_HUMAN    CKR7_MOUSE    
Sequence Titles
CKR7_HUMAN  C-C CHEMOKINE RECEPTOR TYPE 7 PRECURSOR (C-C CKR-7) (CC-CKR-7) (CCR-7) (MIP-3 BETA RECEPTOR) (EBV-INDUCED G PROTEIN-COUPLED RECEPTOR 1) (EBI1) (BLR2) - HOMO SAPIENS (HUMAN). 
CKR7_MOUSE C-C CHEMOKINE RECEPTOR TYPE 7 PRECURSOR (C-C CKR-7) (CC-CKR-7) (CCR-7) (MIP-3 BETA RECEPTOR) (EBV-INDUCED G PROTEIN-COUPLED RECEPTOR 1) (EBI1) - MUS MUSCULUS (MOUSE).
Scan History
SPTR37_9f  1  5    NSINGLE    
Initial Motifs
Motif 1  width=21
Element Seqn Id St Int Rpt
DLGKPMKSVLVVALLVIFQVC CKR7_HUMAN 2 2 -
DPGKPRKNVLVVALLVIFQVC CKR7_MOUSE 2 2 -

Motif 2 width=25
Element Seqn Id St Int Rpt
CQDEVTDDYIGDNTTVDYTLFESLC CKR7_HUMAN 24 1 -
CQDEVTDDYIGENTTVDYTLYESVC CKR7_MOUSE 24 1 -

Motif 3 width=18
Element Seqn Id St Int Rpt
IVQAVSAHRHRARVLLIS CKR7_HUMAN 158 109 -
IVQAVSRHRHRARVLLIS CKR7_MOUSE 158 109 -

Motif 4 width=18
Element Seqn Id St Int Rpt
PELLYSDLQRSSSEQAMR CKR7_HUMAN 192 16 -
PELLYSGLQKNSGEDTLR CKR7_MOUSE 192 16 -

Motif 5 width=18
Element Seqn Id St Int Rpt
CSLITEHVEAFITIQVAQ CKR7_HUMAN 210 0 -
CSLVSAQVEALITIQVAQ CKR7_MOUSE 210 0 -

Motif 6 width=18
Element Seqn Id St Int Rpt
GVVLAQTVANFNITSSTC CKR7_HUMAN 281 53 -
GVVLAQTVANFNITNSSC CKR7_MOUSE 281 53 -

Motif 7 width=18
Element Seqn Id St Int Rpt
CELSKQLNIAYDVTYSLA CKR7_HUMAN 298 -1 -
CETSKQLNIAYDVTYSLA CKR7_MOUSE 298 -1 -

Motif 8 width=15
Element Seqn Id St Int Rpt
SQEQLRQWSSCRHIR CKR7_HUMAN 348 32 -
SQERLRHWSSCRHVR CKR7_MOUSE 348 32 -

Motif 9 width=16
Element Seqn Id St Int Rpt
RRSSMSVEAETTTTFS CKR7_HUMAN 362 -1 -
RNASVSMEAETTTTFS CKR7_MOUSE 362 -1 -
Final Motifs
Motif 1  width=21
Element Seqn Id St Int Rpt
DLGKPMKSVLVVALLVIFQVC CKR7_HUMAN 2 2 -
DPGKPRKNVLVVALLVIFQVC CKR7_MOUSE 2 2 -

Motif 2 width=25
Element Seqn Id St Int Rpt
CQDEVTDDYIGDNTTVDYTLFESLC CKR7_HUMAN 24 1 -
CQDEVTDDYIGENTTVDYTLYESVC CKR7_MOUSE 24 1 -

Motif 3 width=18
Element Seqn Id St Int Rpt
IVQAVSAHRHRARVLLIS CKR7_HUMAN 158 109 -
IVQAVSRHRHRARVLLIS CKR7_MOUSE 158 109 -

Motif 4 width=18
Element Seqn Id St Int Rpt
PELLYSDLQRSSSEQAMR CKR7_HUMAN 192 16 -
PELLYSGLQKNSGEDTLR CKR7_MOUSE 192 16 -

Motif 5 width=18
Element Seqn Id St Int Rpt
CSLITEHVEAFITIQVAQ CKR7_HUMAN 210 0 -
CSLVSAQVEALITIQVAQ CKR7_MOUSE 210 0 -

Motif 6 width=18
Element Seqn Id St Int Rpt
GVVLAQTVANFNITSSTC CKR7_HUMAN 281 53 -
GVVLAQTVANFNITNSSC CKR7_MOUSE 281 53 -

Motif 7 width=18
Element Seqn Id St Int Rpt
CELSKQLNIAYDVTYSLA CKR7_HUMAN 298 -1 -
CETSKQLNIAYDVTYSLA CKR7_MOUSE 298 -1 -

Motif 8 width=15
Element Seqn Id St Int Rpt
SQEQLRQWSSCRHIR CKR7_HUMAN 348 32 -
SQERLRHWSSCRHVR CKR7_MOUSE 348 32 -

Motif 9 width=16
Element Seqn Id St Int Rpt
RRSSMSVEAETTTTFS CKR7_HUMAN 362 -1 -
RNASVSMEAETTTTFS CKR7_MOUSE 362 -1 -