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PR00637

Identifier
BOMBESIN3R  [View Relations]  [View Alignment]  
Accession
PR00637
No. of Motifs
6
Creation Date
08-DEC-1996  (UPDATE 06-JUN-1999)
Title
Type 3 bombesin receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR00358 BOMBESINR
INTERPRO; IPR001560
GCRDB; GCR_0489; GCR_0698; GCR_0961
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Bombesin.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.60-66.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5].
 
Bombesins are peptide neurotransmitters whose biological activity resides
in a common C-terminal sequence, WAXGHXM [6]. In the periphery, bombesin-
related peptides stimulate smooth muscle and glandular secretion. In the
brain, these peptides are believed to play a role in homeostasis, thermo-
regulation and metabolism, and have been reported to elicit analgesia and
excessive grooming, together with central regulation of a variety of
peripheral effects. 
 
Mammalian bombesins are encoded by 2 genes. The preproGRP gene transcript
encodes a precursor of 147 amino acids, which gives GRP and GRP18-27. The
preproNMB gene transcript encodes a precursor of 117 amino acids, which is
metabolised to neuromedin B. Receptors for these peptides have widespread
distribution in peripheral tissue. High levels are found in smooth muscle
and in the brain. 
 
The recently-identified BRS-3 bombesin receptor subtype is found in germ
cells in testis and in uteri of pregnant animals; it is also present in a
variety of lung carcinoma cell lines [6]. The receptor is believed to play
a role in sperm cell division and maturation. Its action is mediated by
association with G proteins that activate a phosphatidylinositol-calcium
second messenger system.
 
BOMBESIN3R is a 6-element fingerprint that provides a signature for type 3
bombesin receptors. The fingerprint was derived from an initial alignment
of 2 sequences: the motifs were drawn from conserved sections within either
loop or N- and C-terminal regions, focusing on those areas of the alignment
that characterise the type 3 bombesin receptors but distinguish them from
the rest of the bombesin family - motifs 1 and 2 lie at the N-terminus;
motif 3 lies in the second external loop; and motifs 4-6 span the C-terminus.
A single iteration on OWL29.0 was required to reach convergence, no further
sequences being identified beyond the starting set.
 
An update on SPTR37_9f identified a true set of 4 sequences.
Summary Information
4 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6444444
5000000
4000000
3000000
2000000
123456
True Positives
BRS3_CAVPO    BRS3_HUMAN    O54798        O88790        
Sequence Titles
BRS3_CAVPO  Bombesin receptor subtype-3 (BRS-3) - Cavia porcellus (Guinea pig). 
BRS3_HUMAN Bombesin receptor subtype-3 (BRS-3) - Homo sapiens (Human).
O54798 BOMBESIN-LIKE RECEPTOR 3 (BOMBESIN RECEPTOR SUBTYPE-3) - MUS MUSCULUS (MOUSE).
O88790 BOMBESIN RECEPTOR SUBTYPE-3 - MUS MUSCULUS (MOUSE).
Scan History
OWL29_0    1  50   NSINGLE    
SPTR37_9f 2 5 NSINGLE
Initial Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
LISITNDTESSSSV BRS3_HUMAN 13 13 -
LISITNDTESSSSV BRS3_CAVPO 13 13 -

Motif 2 width=15
Element Seqn Id St Int Rpt
VVSNDNTNKGWSGDN BRS3_HUMAN 26 -1 -
VVSNDTTNKGWTGDN BRS3_CAVPO 26 -1 -

Motif 3 width=11
Element Seqn Id St Int Rpt
SNVYTFRDPNK BRS3_HUMAN 186 145 -
SNVHTLRDPNK BRS3_CAVPO 186 145 -

Motif 4 width=18
Element Seqn Id St Int Rpt
CKAERPEPPVADTSLTTL BRS3_HUMAN 348 151 -
CKGELPEPPLAATPLNSL BRS3_CAVPO 348 151 -

Motif 5 width=16
Element Seqn Id St Int Rpt
LAVMGTVPGTGSIQMS BRS3_HUMAN 365 -1 -
LAVMGRVSGTENTHIS BRS3_CAVPO 365 -1 -

Motif 6 width=17
Element Seqn Id St Int Rpt
SEISVTSFTGCSVKQAE BRS3_HUMAN 380 -1 -
SEIGVASFIGRPMKKEE BRS3_CAVPO 380 -1 -
Final Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
LISITNDTETSSSV O54798 13 13 -
LISITNDTETSSSV O88790 13 13 -
LISITNDTESSSSV BRS3_HUMAN 13 13 -
LISITNDTESSSSV BRS3_CAVPO 13 13 -

Motif 2 width=15
Element Seqn Id St Int Rpt
VVSNDTTHKGWTGDN O54798 26 -1 -
VAPNDTTHKGWTGDN O88790 26 -1 -
VVSNDNTNKGWSGDN BRS3_HUMAN 26 -1 -
VVSNDTTNKGWTGDN BRS3_CAVPO 26 -1 -

Motif 3 width=11
Element Seqn Id St Int Rpt
SNVYTFQDPNR O54798 186 145 -
SNVYTFQDPNR O88790 186 145 -
SNVYTFRDPNK BRS3_HUMAN 186 145 -
SNVHTLRDPNK BRS3_CAVPO 186 145 -

Motif 4 width=18
Element Seqn Id St Int Rpt
LKAEQPEPPLGDIPLNNL O54798 348 151 -
LKAEQPEPPLGDIPLNNL O88790 348 151 -
CKAERPEPPVADTSLTTL BRS3_HUMAN 348 151 -
CKGELPEPPLAATPLNSL BRS3_CAVPO 348 151 -

Motif 5 width=16
Element Seqn Id St Int Rpt
LTVMGRVPATGSAHVS O54798 365 -1 -
LTVMGRVPATGSAHVS O88790 365 -1 -
LAVMGTVPGTGSIQMS BRS3_HUMAN 365 -1 -
LAVMGRVSGTENTHIS BRS3_CAVPO 365 -1 -

Motif 6 width=17
Element Seqn Id St Int Rpt
SEISVTLFSGSSAKKGE O54798 380 -1 -
SEISVTLFSGSSAKKGE O88790 380 -1 -
SEISVTSFTGCSVKQAE BRS3_HUMAN 380 -1 -
SEIGVASFIGRPMKKEE BRS3_CAVPO 380 -1 -