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PR00602

Identifier
BARWIN  [View Relations]  [View Alignment]  
Accession
PR00602
No. of Motifs
4
Creation Date
25-OCT-1996  (UPDATE 06-JUN-1999)
Title
Barwin domain signature
Database References

PROSITE; PS00771 BARWIN_1; PS00772 BARWIN_2
BLOCKS; BL00771
PFAM; PF00967 Barwin
INTERPRO; IPR001153
PDB; 1BW4
SCOP; 1BW4
CATH; 1BW4
Literature References
1. SVENSSON, B., SVENDSEN, I., HOJRUP, P., ROEPSTORFF, P., LUDVIGSEN, S.
AND POULSEN, F.M.
Primary structure of barwin: a barley seed protein closely related to the
C-terminal domain of proteins encoded by wound-induced plant genes.
BIOCHEMISTRY 31 8767-8770 (1992).
 
2. LUDVIGSEN, S. AND POULSEN, F.M.
Secondary structure in solution of barwin from barley seed using 1H nuclear
magnetic resonance spectroscopy.
BIOCHEMISTRY 31 8771-8782 (1992).

Documentation
Barwin is a basic protein isolated from aqueous extracts of barley seeds
[1]. It is 125 amino acids in length, and contains 6 cysteine residues that 
combine to form 3 disulphide bridges [1,2]. Comparative analysis shows the
sequence to be highly similar to a 122 amino acid stretch in the C-terminal
of the products of 2 wound-induced genes (win1 and win2) from potato, the 
product of the hevein gene of rubber trees, and pathogenesis-related 
protein 4 from tobacco [1,2]. The high levels of similarity to these 
proteins, and its ability to bind saccharides, suggest that the barwin 
domain may be involved in a common defense mechanism in plants [1,2].
 
NMR analysis shows that the secondary structure of the barwin domain is 
dominated by a large 4-stranded anti-parallel beta-sheet and a small 
parallel beta-sheet [2]. Three short alpha-helices are also present, the
remaining sequence forming loops and coils with no regular secondary
structure elements [2].
 
BARWIN is a 4-element fingerprint that provides a signature for the barwin
domain. The fingerprint was derived from an initial alignment of 8 sequences:
the motifs were drawn from conserved regions spanning virtually the full
alignment length - motifs 2 and 4 include the regions encoded by PROSITE
patterns BARWIN_1 (PS00771) and BARWIN_2 (PS00772), which contain conserved
Cys residues. Two iterations on OWL28.2 were required to reach convergence,
at which point a true set comprising 14 sequences was identified.
 
An update on SPTR37_9f identified a true set of 15 sequences.
Summary Information
15 codes involving  4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
415151515
30000
20000
1234
True Positives
BARW_HORVU    HEVE_HEVBR    HEVL_ARATH    O48880        
O64392 O64393 O81228 P93180
PR4A_TOBAC PR4B_TOBAC PRP2_LYCES Q40558
Q41802 WIN1_SOLTU WIN2_SOLTU
Sequence Titles
BARW_HORVU  BARWIN - HORDEUM VULGARE (BARLEY).            
HEVE_HEVBR HEVEIN PRECURSOR (MAJOR HEVEIN) - HEVEA BRASILIENSIS (PARA RUBBER TREE).
HEVL_ARATH HEVEIN-LIKE PROTEIN PRECURSOR - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O48880 PATHOGENESIS-RELATED PROTEIN-4 - DIOSCOREA BULBIFERA.
O64392 WHEATWIN1 PRECURSOR (PROTEIN 0.14) - TRITICUM AESTIVUM (WHEAT).
O64393 WHEATWIN2 PRECURSOR - TRITICUM AESTIVUM (WHEAT).
O81228 PR-4 TYPE PROTEIN - VITIS VINIFERA (GRAPE).
P93180 PATHOGENESIS-RELATED PROTEIN 4 PRECURSOR - HORDEUM VULGARE (BARLEY).
PR4A_TOBAC PATHOGENESIS-RELATED PROTEIN PR-4A PRECURSOR - NICOTIANA TABACUM (COMMON TOBACCO).
PR4B_TOBAC PATHOGENESIS-RELATED PROTEIN PR-4B PRECURSOR - NICOTIANA TABACUM (COMMON TOBACCO).
PRP2_LYCES PATHOGENESIS-RELATED PROTEIN P2 PRECURSOR - LYCOPERSICON ESCULENTUM (TOMATO).
Q40558 PATHOGENESIS-RELATED PROTEIN 4A PRECURSOR - NICOTIANA TABACUM (COMMON TOBACCO).
Q41802 DEFENCE-RELATED PROTEIN PRECURSOR - ZEA MAYS (MAIZE).
WIN1_SOLTU WOUND-INDUCED PROTEIN WIN1 PRECURSOR - SOLANUM TUBEROSUM (POTATO).
WIN2_SOLTU WOUND-INDUCED PROTEIN WIN2 PRECURSOR - SOLANUM TUBEROSUM (POTATO).
Scan History
OWL28_2    2  100  NSINGLE    
SPTR37_9f 2 16 NSINGLE
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
KPLSWRKKYGWTAFCGPVG WIN1_SOLTU 113 113 -
KPYAWRSKYGWTAFCGPAG HEVL_ARATH 107 107 -
KPYSWRSKYGWTAFCGPVG WIN_SOYBN 1 1 -
KPYSWRSKYGWTAFCGPVG HEVE_HEVBR 103 103 -
KPLSWRSKYGWTAFCGPAG BARW_HORVU 38 38 -
KPLAWRQKYGWTAFCGPAG PR4A_TOBAC 61 61 -
KPLAWRQKYGWTAFCGPAG PR4B_TOBAC 61 61 -
KPYAWRSKYGWTAFCGPVG WIN2_SOLTU 112 112 -

Motif 2 width=19
Element Seqn Id St Int Rpt
PRGQASCGKCLRVKNTRTN HEVL_ARATH 126 0 -
PRGRDSCGKCLRVTNTGTG WIN_SOYBN 20 0 -
PRGQAACGKCLRVTNPATG BARW_HORVU 57 0 -
PRGQDSCGRCLRVTNTGTG PR4A_TOBAC 80 0 -
PRGQDSCGRCLRVTNTGTG PR4B_TOBAC 80 0 -
PRGRDSCGKCLRVTNTRTG WIN2_SOLTU 131 0 -
PRGRDSCGKCLRVTNTRTG WIN1_SOLTU 132 0 -
AHGQSSCGKCLSVTNTGTG HEVE_HEVBR 122 0 -

Motif 3 width=19
Element Seqn Id St Int Rpt
ANTIVRIVDQCSNGGLDLD WIN_SOYBN 39 0 -
AQITARIVDQCANGGLDLD BARW_HORVU 76 0 -
TQTTVRIVDQCSNGGLDLD PR4A_TOBAC 99 0 -
TQATVRIVDQCSNGGLDLD PR4B_TOBAC 99 0 -
AQTTVRIVDQCSNGGLDLD WIN2_SOLTU 150 0 -
AQTTVRIVDQCSNGGLDLD WIN1_SOLTU 151 0 -
AAVTVRIVDQCSNGGLDLD HEVL_ARATH 145 0 -
AKTTVRIVDQCSNGGLDLD HEVE_HEVBR 141 0 -

Motif 4 width=25
Element Seqn Id St Int Rpt
MFNQIDTDGFGYQQGHLIVDYQFVD HEVL_ARATH 166 2 -
VFRQLDTDGKGYERGHITVNYQFVD HEVE_HEVBR 162 2 -
VFTKIDTNGIGYQQGHLNVNYQFVD BARW_HORVU 98 3 -
VFNRIDTDGRGYQQGHLIVNYQFVD WIN_SOYBN 60 2 -
VFNQLDTNGVGYQQGHLTVNYEFVN PR4A_TOBAC 120 2 -
VFNQLDTNGLGYQQGHLIVNYEFVN PR4B_TOBAC 120 2 -
VFQQIDTDGVGNQQGHLIVNYQFVN WIN2_SOLTU 171 2 -
VFRQIDTDGNGNHQGHLIVNYQFVD WIN1_SOLTU 172 2 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
KPLSWRSKYGWTAFCGPAG BARW_HORVU 38 38 -
KPLAWRQKYGWTAFCGPAG PR4A_TOBAC 61 61 -
KPLAWRQKYGWTAFCGPAG PR4B_TOBAC 61 61 -
KPLAWRQKYGWTAFCGPAG Q40558 61 61 -
KPLSWRSKYGWTAFCGPAG O64393 61 61 -
KPLSWRSKYGWTAFCGPAG O64392 59 59 -
KPYAWRSKYGWTAFCGPVG WIN2_SOLTU 112 112 -
KPLSWRSKYGWTAFCGPAG P93180 59 59 -
KPLSWRKKYGWTAFCGPVG WIN1_SOLTU 113 113 -
QPLAWRSKYGWTAFCGPSG O81228 57 57 -
KPYAWRSKYGWTAFCGPAG HEVL_ARATH 107 107 -
KPLAWRQKYGWTAFCGPAG O48880 65 65 -
KPLEWRRRYGWTAFCGPAG PRP2_LYCES 59 59 -
KPYSWRSKYGWTAFCGPVG HEVE_HEVBR 103 103 -
KPLSWRQKHGWTAFCGPAG Q41802 60 60 -

Motif 2 width=19
Element Seqn Id St Int Rpt
PRGQAACGKCLRVTNPATG BARW_HORVU 57 0 -
PRGQDSCGRCLRVTNTGTG PR4A_TOBAC 80 0 -
PRGQDSCGRCLRVTNTGTG PR4B_TOBAC 80 0 -
PRGQVSCGRCLRVTNTGTG Q40558 80 0 -
AHGQAACGKCLRVTNPATG O64393 80 0 -
AHGQASCGKCLQVTNPATG O64392 78 0 -
PRGRDSCGKCLRVTNTRTG WIN2_SOLTU 131 0 -
PLGQAACGKCLRVTNPATG P93180 78 0 -
PRGRDSCGKCLRVTNTRTG WIN1_SOLTU 132 0 -
PTGQAACGKCLSVTNTATG O81228 76 0 -
PRGQASCGKCLRVKNTRTN HEVL_ARATH 126 0 -
PTGQDACGKCLLVTNTKTN O48880 84 0 -
PTGQASCGRCLRVTNTGTG PRP2_LYCES 78 0 -
AHGQSSCGKCLSVTNTGTG HEVE_HEVBR 122 0 -
QKGPGRCGKCIRVKNRATG Q41802 79 0 -

Motif 3 width=19
Element Seqn Id St Int Rpt
AQITARIVDQCANGGLDLD BARW_HORVU 76 0 -
TQTTVRIVDQCSNGGLDLD PR4A_TOBAC 99 0 -
TQATVRIVDQCSNGGLDLD PR4B_TOBAC 99 0 -
TQTTVRIVDQCSNGGLDLD Q40558 99 0 -
AQITARIVDQCANGGLDLD O64393 99 0 -
AQITARIVDQCANGGLDLD O64392 97 0 -
AQTTVRIVDQCSNGGLDLD WIN2_SOLTU 150 0 -
AQITARIVDKCANGGLDLD P93180 97 0 -
AQTTVRIVDQCSNGGLDLD WIN1_SOLTU 151 0 -
TQATVRIVDQCSNGGLDLD O81228 95 0 -
AAVTVRIVDQCSNGGLDLD HEVL_ARATH 145 0 -
AQATVRIVDQCSNGGLDLD O48880 103 0 -
TQETVRIVDQCRNGGLDLD PRP2_LYCES 97 0 -
AKTTVRIVDQCSNGGLDLD HEVE_HEVBR 141 0 -
ASIVARIVDQCSNGGLDLD Q41802 98 0 -

Motif 4 width=25
Element Seqn Id St Int Rpt
VFTKIDTNGIGYQQGHLNVNYQFVD BARW_HORVU 98 3 -
VFNQLDTNGVGYQQGHLTVNYEFVN PR4A_TOBAC 120 2 -
VFNQLDTNGLGYQQGHLIVNYEFVN PR4B_TOBAC 120 2 -
VFNQLDTNGVGYQQGHLTVNYEFVN Q40558 120 2 -
VFTKIDTNGIGYQQGHLNVNYQFVD O64393 121 3 -
VFTKIDTNGIGYQQGHLNVNYQFVD O64392 119 3 -
VFQQIDTDGVGNQQGHLIVNYQFVN WIN2_SOLTU 171 2 -
VFAKIDTNGIGFQQGHLNVNYQFVD P93180 119 3 -
VFRQIDTDGNGNHQGHLIVNYQFVD WIN1_SOLTU 172 2 -
VFNKLDTNGAGYNQGHLIVNYEFVD O81228 116 2 -
MFNQIDTDGFGYQQGHLIVDYQFVD HEVL_ARATH 166 2 -
VFEQIDTDKSGYAQGHLIVNYEFVN O48880 124 2 -
VFNRLDTNGLGYQRGNLNVNYEFVN PRP2_LYCES 118 2 -
VFRQLDTDGKGYERGHITVNYQFVD HEVE_HEVBR 162 2 -
VFKKIDTNGQGYQMGHLNVDYQFVA Q41802 120 3 -