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PR00591

Identifier
SOMATOSTTN5R  [View Relations]  [View Alignment]  
Accession
PR00591
No. of Motifs
5
Creation Date
10-SEP-1996  (UPDATE 07-JUN-1999)
Title
Somatostatin receptor type 5 signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR00246 SOMATOSTATNR
INTERPRO; IPR001184
GCRDB; GCR_0631; GCR_0801; GCR_0514; GCR_0829
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Somatostatin.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, PP.252-260.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Somatostatin is a neurotransmitter/hormone with a wide range of biologial
functions [6]. It has an important role in the neuroendocrine system and
inhibits secretion of growth hormone and prolactin in the anterior
pituitary. It also inhibits secretion in the intestine (including gastric
acid in the stomach), pancreatic acinar cells and pancreatic beta-cells,
stimulates absorption in the intestine, and modulates smooth muscle 
contractility. In the CNS, it is a neurotransmitter activating a hyper-
polarising K+ current and inhibiting Ca2+ influx, and is believed to
play important roles in regulating locomotor activity and cognitive
function. Receptor subtypes were originally proposed on the basis of
functional and radio-ligand binding studies, and cloning has now confirmed
the presence of at least 4 subtypes.
 
SOMATOSTTN5R is a 5-element fingerprint that provides a signature for the
type 5 somatostatin receptors. The fingerprint was derived from an initial
alignment of 3 sequences: the motifs were drawn from conserved sections
within either loop or N- and C-terminal regions, focusing on those areas
of the alignment that characterise the type 5 somatostatin receptors but
distinguish them from the rest of the rhodopsin-like superfamily - motif 1
lies at the N-terminus; motif 2 was drawn from the N-terminal half of TM
domain 1; motifs 3 and 4 span the third cytoplasmic and external loops
res.; and motif 5 lies at the C-terminus. A single iteration on OWL28.1 was
required to reach convergence, no further sequences being identified beyond
the starting set. 
 
An update on SPTR37_9f identified a true set of 3 sequences.
Summary Information
3 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
533333
400000
300000
200000
12345
True Positives
SSR5_HUMAN    SSR5_MOUSE    SSR5_RAT      
Sequence Titles
SSR5_HUMAN  Somatostatin receptor type 5 (SS5R) - Homo sapiens (Human). 
SSR5_MOUSE Somatostatin receptor type 5 (SS5R) - Mus musculus (Mouse).
SSR5_RAT Somatostatin receptor type 5 (SS5R) - Rattus norvegicus (Rat).
Scan History
OWL28_1    1  25   NSINGLE    
SPTR37_9f 2 4 NSINGLE
Initial Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
PLFPASTPSWNASS SSR5_HUMAN 3 3 -
PLSLASTPSWNASA SSR5_RAT 3 3 -
PLFPASTPSWNASS HUMSSTR5 3 3 -

Motif 2 width=15
Element Seqn Id St Int Rpt
MGARAVLVPVLYLLV SSR5_RAT 33 16 -
AGARAVLVPVLYLLV HUMSSTR5 36 19 -
AGARAVLVPVLYLLV SSR5_HUMAN 36 19 -

Motif 3 width=15
Element Seqn Id St Int Rpt
KVKAAGMRVGSSRRR SSR5_RAT 226 178 -
KVRAAGVRVGCVRRR HUMSSTR5 227 176 -
KVRAAGVRVGCVRRR SSR5_HUMAN 227 176 -

Motif 4 width=17
Element Seqn Id St Int Rpt
LAVALPQEPASAGLYFF HUMSSTR5 272 30 -
LAFTLPEEPTSAGLYFF SSR5_RAT 272 31 -
LAVALPQEPASAGLYFF SSR5_HUMAN 272 30 -

Motif 5 width=15
Element Seqn Id St Int Rpt
GAKDADATEPRPDRI HUMSSTR5 326 37 -
GAKDADATEPRPDRI SSR5_HUMAN 326 37 -
GMEDADAIEPRPDKS SSR5_RAT 326 37 -
Final Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
PLSLASTPSWNASA SSR5_MOUSE 3 3 -
PLSLASTPSWNASA SSR5_RAT 3 3 -
PLFPASTPSWNASS SSR5_HUMAN 3 3 -

Motif 2 width=15
Element Seqn Id St Int Rpt
MGARAVLVPVLYLLV SSR5_MOUSE 33 16 -
MGARAVLVPVLYLLV SSR5_RAT 33 16 -
AGARAVLVPVLYLLV SSR5_HUMAN 36 19 -

Motif 3 width=15
Element Seqn Id St Int Rpt
KVKAAGMRVGSSRRR SSR5_MOUSE 225 177 -
KVKAAGMRVGSSRRR SSR5_RAT 226 178 -
KVRAAGVRVGCVRRR SSR5_HUMAN 227 176 -

Motif 4 width=17
Element Seqn Id St Int Rpt
LAFTLPEEPTSAGLYFF SSR5_MOUSE 271 31 -
LAFTLPEEPTSAGLYFF SSR5_RAT 272 31 -
LAVALPQEPASAGLYFF SSR5_HUMAN 272 30 -

Motif 5 width=15
Element Seqn Id St Int Rpt
GVEDADAIEPRPDKS SSR5_MOUSE 325 37 -
GMEDADAIEPRPDKS SSR5_RAT 326 37 -
GAKDADATEPRPDRI SSR5_HUMAN 326 37 -