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PR00589

Identifier
SOMATOSTTN3R  [View Relations]  [View Alignment]  
Accession
PR00589
No. of Motifs
7
Creation Date
10-SEP-1996  (UPDATE 07-JUN-1999)
Title
Somatostatin receptor type 3 signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR00246 SOMATOSTATNR
INTERPRO; IPR001856
GCRDB; GCR_0502; GCR_0470; GCR_0452; GCR_0630
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Somatostatin.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, PP.252-260.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Somatostatin is a neurotransmitter/hormone with a wide range of biologial
functions [6]. It has an important role in the neuroendocrine system and
inhibits secretion of growth hormone and prolactin in the anterior
pituitary. It also inhibits secretion in the intestine (including gastric
acid in the stomach), pancreatic acinar cells and pancreatic beta-cells,
stimulates absorption in the intestine, and modulates smooth muscle 
contractility. In the CNS, it is a neurotransmitter activating a hyper-
polarising K+ current and inhibiting Ca2+ influx, and is believed to
play important roles in regulating locomotor activity and cognitive
function. Receptor subtypes were originally proposed on the basis of
functional and radio-ligand binding studies, and cloning has now confirmed
the presence of at least 4 subtypes.
 
mRNA for the SS3 receptor is widely distributed in mouse brain, with high
levels in the forebrain, hippocampus and amygdala; moderate levels are also
present in the substantia nigra [6]. The receptors inhibit adenylyl cyclase
through a pertussis-toxin-sensitive G protein, probably belonging to the 
Gi/Go class [6].  
 
SOMATOSTTN3R is a 7-element fingerprint that provides a signature for the
type 3 somatostatin receptors. The fingerprint was derived from an initial
alignment of 3 sequences: the motifs were drawn from conserved sections
within either loop or N- and C-terminal regions, focusing on those areas
of the alignment that characterise the type 3 somatostatin receptors
but distinguish them from the rest of the rhodopsin-like superfamily -
motifs 1 and 2 lie at the N-terminus; motif 3 spans the second external 
loop; motif 4 lies in the third cytoplasmic loop; motif 5 spans the
third external loop; and motifs 6 and 7 lie at the C-terminus. A single
iteration on OWL28.1 was required to reach convergence, no further sequences
being identified beyond the starting set. Three partial matches were also
found, all of which are type 5 somatostatin receptors that make weak matches
with motifs 3-5. 
 
An update on SPTR37_9f identified a true set of 3 sequences.
Summary Information
3 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
73333333
60000000
50000000
40000000
30000000
20000000
1234567
True Positives
SSR3_HUMAN    SSR3_MOUSE    SSR3_RAT      
Sequence Titles
SSR3_HUMAN  Somatostatin receptor type 3 (SS3R) (SSR-28) - Homo sapiens (Human). 
SSR3_MOUSE Somatostatin receptor type 3 (SS3R) (SSR-28) - Mus musculus (Mouse).
SSR3_RAT Somatostatin receptor type 3 (SS3R) (SSR-28) - Rattus norvegicus (Rat).
Scan History
OWL28_1    1  100  NSINGLE    
SPTR37_9f 2 4 NSINGLE
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
TLDPGNASSAWPLDTSLGN SSR3_RAT 13 13 -
TLDPGNTSSTWPLDTTLGN SSR3_MOUSE 13 13 -
TSEPENASSAWPPDATLGN SSR3_HUMAN 12 12 -

Motif 2 width=18
Element Seqn Id St Int Rpt
SAGTSLAGLAVSGILISL SSR3_RAT 33 1 -
SAGASLTGLAVSGILISL SSR3_MOUSE 33 1 -
SAGPSPAGLAVSGVLIPL SSR3_HUMAN 32 1 -

Motif 3 width=19
Element Seqn Id St Int Rpt
VVVFSGVPRGMSTCHMQWP SSR3_RAT 179 128 -
VVVFSGVPRGMSTCHMQWP SSR3_MOUSE 179 128 -
VVVFSGVPRGMSTCHMQWP SSR3_HUMAN 178 128 -

Motif 4 width=17
Element Seqn Id St Int Rpt
VVKVRSTTRRVRAPSCQ SSR3_RAT 231 33 -
VVKVRSTTRRVRAPSCQ SSR3_MOUSE 231 33 -
VVKVRSAGRRVWAPSCQ SSR3_HUMAN 230 33 -

Motif 5 width=17
Element Seqn Id St Int Rpt
VVCPLPEEPAFFGLYFL SSR3_RAT 290 42 -
VVCPLPEEPAFFGLYFL SSR3_MOUSE 290 42 -
VVCPLPEEPAFFGLYFL SSR3_HUMAN 281 34 -

Motif 6 width=19
Element Seqn Id St Int Rpt
QGFRRILLRPSRRVRSQEP SSR3_RAT 331 24 -
QGFRRILLRPSRRIRSQEP SSR3_MOUSE 331 24 -
QGFRRVLLRPSRRVRSQEP SSR3_HUMAN 322 24 -

Motif 7 width=18
Element Seqn Id St Int Rpt
KEQQLLPQEATAGDKAST SSR3_RAT 407 57 -
KEQQLLPQEATAGDKAST SSR3_MOUSE 407 57 -
KEQQLLPQEASTGEKSST SSR3_HUMAN 395 54 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
TLDPGNASSAWPLDTSLGN SSR3_RAT 13 13 -
TLDPGNTSSTWPLDTTLGN SSR3_MOUSE 13 13 -
TSEPENASSAWPPDATLGN SSR3_HUMAN 12 12 -

Motif 2 width=18
Element Seqn Id St Int Rpt
SAGTSLAGLAVSGILISL SSR3_RAT 33 1 -
SAGASLTGLAVSGILISL SSR3_MOUSE 33 1 -
SAGPSPAGLAVSGVLIPL SSR3_HUMAN 32 1 -

Motif 3 width=19
Element Seqn Id St Int Rpt
VVVFSGVPRGMSTCHMQWP SSR3_RAT 179 128 -
VVVFSGVPRGMSTCHMQWP SSR3_MOUSE 179 128 -
VVVFSGVPRGMSTCHMQWP SSR3_HUMAN 178 128 -

Motif 4 width=17
Element Seqn Id St Int Rpt
VVKVRSTTRRVRAPSCQ SSR3_RAT 231 33 -
VVKVRSTTRRVRAPSCQ SSR3_MOUSE 231 33 -
VVKVRSAGRRVWAPSCQ SSR3_HUMAN 230 33 -

Motif 5 width=17
Element Seqn Id St Int Rpt
VVCPLPEEPAFFGLYFL SSR3_RAT 290 42 -
VVCPLPEEPAFFGLYFL SSR3_MOUSE 290 42 -
VVCPLPEEPAFFGLYFL SSR3_HUMAN 281 34 -

Motif 6 width=19
Element Seqn Id St Int Rpt
QGFRRILLRPSRRVRSQEP SSR3_RAT 331 24 -
QGFRRILLRPSRRIRSQEP SSR3_MOUSE 331 24 -
QGFRRVLLRPSRRVRSQEP SSR3_HUMAN 322 24 -

Motif 7 width=18
Element Seqn Id St Int Rpt
KEQQLLPQEATAGDKAST SSR3_RAT 407 57 -
KEQQLLPQEATAGDKAST SSR3_MOUSE 407 57 -
KEQQLLPQEASTGEKSST SSR3_HUMAN 395 54 -