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PR00588

Identifier
SOMATOSTTN2R  [View Relations]  [View Alignment]  
Accession
PR00588
No. of Motifs
6
Creation Date
10-SEP-1996  (UPDATE 07-JUN-1999)
Title
Somatostatin receptor type 2 signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR00246 SOMATOSTATNR
INTERPRO; IPR002074
GCRDB; GCR_0274; GCR_0566; GCR_0375; GCR_0416; GCR_0796; GCR_0271

GCRDB; GCR_0190
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Somatostatin.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, PP.252-260.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Somatostatin is a neurotransmitter/hormone with a wide range of biologial
functions [6]. It has an important role in the neuroendocrine system and
inhibits secretion of growth hormone and prolactin in the anterior
pituitary. It also inhibits secretion in the intestine (including gastric
acid in the stomach), pancreatic acinar cells and pancreatic beta-cells,
stimulates absorption in the intestine, and modulates smooth muscle 
contractility. In the CNS, it is a neurotransmitter activating a hyper-
polarising K+ current and inhibiting Ca2+ influx, and is believed to
play important roles in regulating locomotor activity and cognitive
function. Receptor subtypes were originally proposed on the basis of
functional and radio-ligand binding studies, and cloning has now confirmed
the presence of at least 4 subtypes.
 
High levels of mRNA for human SS2 receptors have been found in the brain,
kidney and pituitary, with lower levels in the jejunum, pancreas, colon and 
liver [6]. The receptor is coupled to Go and Gi3 and is linked to inhibition
of adenylyl cyclase [6].  
 
SOMATOSTTN2R is a 6-element fingerprint that provides a signature for the
type 2 somatostatin receptors. The fingerprint was derived from an initial
alignment of 5 sequences: the motifs were drawn from conserved sections
within either loop or N- and C-terminal regions, focusing on those areas
of the alignment that characterise the type 2 somatostatin receptors
but distinguish them from the rest of the rhodopsin-like superfamily -
motifs 1 and 2 lie at the N-terminus; motifs 3 and 4 span the second and
third external loops res.; and motifs 5 and 6 lie at the C-terminus. A
single iteration on OWL28.1 was required to reach convergence, no further
sequences being identified beyond the starting set. A single partial match
was also found, MMSSTR2, a murine type 2 somatostatin receptor that fails
to match the C-terminal motifs. 
 
An update on SPTR37_9f identified a true set of 5 sequences.
Summary Information
5 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6555555
5000000
4000000
3000000
2000000
123456
True Positives
SSR2_BOVIN    SSR2_HUMAN    SSR2_MOUSE    SSR2_PIG      
SSR2_RAT
Sequence Titles
SSR2_BOVIN  Somatostatin receptor type 2 (SS2R) (SRIF-1) - Bos taurus (Bovine). 
SSR2_HUMAN Somatostatin receptor type 2 (SS2R) (SRIF-1) - Homo sapiens (Human).
SSR2_MOUSE Somatostatin receptor type 2 (SS2R) (SRIF-1) - Mus musculus (Mouse).
SSR2_PIG Somatostatin receptor type 2 (SS2R) (SRIF-1) - Sus scrofa (Pig).
SSR2_RAT Somatostatin receptor type 2 (SS2R) (SRIF-1) - Rattus norvegicus (Rat).
Scan History
OWL28_1    1  50   NSINGLE    
SPTR37_9f 2 7 NSINGLE
Initial Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
LNGSQPWLSSPFDLNGSV SSR2_PIG 8 8 -
LNGSQVWVSSPFDLNGSL SSR2_MOUSE 8 8 -
FNGSQVWIPSPFDLNGSL SSR2_RAT 8 8 -
LNGSHTWLSIPFDLNGSV SSR2_HUMAN 8 8 -
LNETQPWLTTPFDLNGSV SSR2_BOVIN 7 7 -

Motif 2 width=20
Element Seqn Id St Int Rpt
ATANSSNQTEPYYDLTSNAV SSR2_PIG 26 0 -
GPSNGSNQTEPYYDMTSNAV SSR2_MOUSE 26 0 -
GPSNGSNQTEPYYDMTSNAV SSR2_RAT 26 0 -
VSTNTSNQTEPYYDLTSNAV SSR2_HUMAN 26 0 -
GAANISNQTEPYYDLASNVV SSR2_BOVIN 25 0 -

Motif 3 width=16
Element Seqn Id St Int Rpt
YAGLRSNQWGRSSCTI SSR2_PIG 180 134 -
YAGLRSNQWGRSSCTI SSR2_MOUSE 180 134 -
YAGLRSNQWGRSSCTI SSR2_RAT 180 134 -
YAGLRSNQWGRSSCTI SSR2_HUMAN 180 134 -
YAGLRSNQWGRSSCTI SSR2_BOVIN 179 134 -

Motif 4 width=20
Element Seqn Id St Int Rpt
VSSVSVAISPTPALKGMFDF SSR2_PIG 277 81 -
VSSVSVAISPTPALKGMFDF SSR2_MOUSE 277 81 -
VSSVSVAISPTPALKGMFDF SSR2_RAT 277 81 -
VSSVSMAISPTPALKGMFDF SSR2_HUMAN 277 81 -
VSSVSVAISPTPALKGMFDF SSR2_BOVIN 276 81 -

Motif 5 width=18
Element Seqn Id St Int Rpt
VSGTDDGERSDSKQDKSR SSR2_PIG 332 35 -
VSGTEDGERSDSKQDKSR SSR2_MOUSE 332 35 -
VSGAEDGERSDSKQDKSR SSR2_RAT 332 35 -
VSGTDDGERSDSKQDKSR SSR2_HUMAN 332 35 -
VSGTDDGERSDSKQDKSR SSR2_BOVIN 331 35 -

Motif 6 width=19
Element Seqn Id St Int Rpt
LNETTETQRTLLNGDLQTS SSR2_PIG 350 0 -
LNETTETQRTLLNGDLQTS SSR2_MOUSE 350 0 -
LNETTETQRTLLNGDLQTS SSR2_RAT 350 0 -
LNETTETQRTLLNGDLQTS SSR2_HUMAN 350 0 -
LNETTETQRTLLNGDLQTS SSR2_BOVIN 349 0 -
Final Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
LNGSQPWLSSPFDLNGSV SSR2_PIG 8 8 -
LNGSQVWVSSPFDLNGSL SSR2_MOUSE 8 8 -
FNGSQVWIPSPFDLNGSL SSR2_RAT 8 8 -
LNGSHTWLSIPFDLNGSV SSR2_HUMAN 8 8 -
LNETQPWLTTPFDLNGSV SSR2_BOVIN 7 7 -

Motif 2 width=20
Element Seqn Id St Int Rpt
ATANSSNQTEPYYDLTSNAV SSR2_PIG 26 0 -
GPSNGSNQTEPYYDMTSNAV SSR2_MOUSE 26 0 -
GPSNGSNQTEPYYDMTSNAV SSR2_RAT 26 0 -
VSTNTSNQTEPYYDLTSNAV SSR2_HUMAN 26 0 -
GAANISNQTEPYYDLASNVV SSR2_BOVIN 25 0 -

Motif 3 width=16
Element Seqn Id St Int Rpt
YAGLRSNQWGRSSCTI SSR2_PIG 180 134 -
YAGLRSNQWGRSSCTI SSR2_MOUSE 180 134 -
YAGLRSNQWGRSSCTI SSR2_RAT 180 134 -
YAGLRSNQWGRSSCTI SSR2_HUMAN 180 134 -
YAGLRSNQWGRSSCTI SSR2_BOVIN 179 134 -

Motif 4 width=20
Element Seqn Id St Int Rpt
VSSVSVAISPTPALKGMFDF SSR2_PIG 277 81 -
VSSVSVAISPTPALKGMFDF SSR2_MOUSE 277 81 -
VSSVSVAISPTPALKGMFDF SSR2_RAT 277 81 -
VSSVSMAISPTPALKGMFDF SSR2_HUMAN 277 81 -
VSSVSVAISPTPALKGMFDF SSR2_BOVIN 276 81 -

Motif 5 width=18
Element Seqn Id St Int Rpt
VSGTDDGERSDSKQDKSR SSR2_PIG 332 35 -
VSGTEDGERSDSKQDKSR SSR2_MOUSE 332 35 -
VSGAEDGERSDSKQDKSR SSR2_RAT 332 35 -
VSGTDDGERSDSKQDKSR SSR2_HUMAN 332 35 -
VSGTDDGERSDSKQDKSR SSR2_BOVIN 331 35 -

Motif 6 width=19
Element Seqn Id St Int Rpt
LNETTETQRTLLNGDLQTS SSR2_PIG 350 0 -
LNETTETQRTLLNGDLQTS SSR2_MOUSE 350 0 -
LNETTETQRTLLNGDLQTS SSR2_RAT 350 0 -
LNETTETQRTLLNGDLQTS SSR2_HUMAN 350 0 -
LNETTETQRTLLNGDLQTS SSR2_BOVIN 349 0 -