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PR00577

Identifier
OPSINRH3RH4  [View Relations]  [View Alignment]  
Accession
PR00577
No. of Motifs
7
Creation Date
11-SEP-1996  (UPDATE 07-JUN-1999)
Title
Opsin RH3/RH4 signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR00238 OPSIN
INTERPRO; IPR000856
GCRDB; GCR_0513; GCR_0780; GCR_0781; GCR_0031; GCR_0030; GCR_0032

GCRDB; GCR_0430
Literature References
1. APPLEBURY, M.L. AND HARGRAVE, P.A.
Molecular biology of the visual pigments.
VISION RES. 26(12) 1881-1895 (1986).
 
2. FRYXELL, K.J. AND MEYEROWITZ, E.M.
The evolution of rhodopsins and neurotransmitter receptors.
J.MOL.EVOL. 33(4) 367-378 (1991).
 
3. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
4. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994). 
 
5. FRYXELL, K.J. AND MEYEROWITZ, E.M.
An opsin gene that is expressed only in the R7 photoreceptor cell of
Drosophila.
EMBO J. 6(2) 443-451 (1987).
 
6. ZUKER, C.S., MONTELL, C., JONES, K., LAVERTY, T. AND RUBIN, G.M.J. 
A rhodopsin gene expressed in photoreceptor cell R7 of the Drosophila
eye - homologies with other signal-transducing molecules.
NEUROSCIENCE 7(5) 1550-1557 (1987).
 
7. MONTELL, C., JONES, K., ZUKER, C.S. AND RUBIN, G.M.J. 
A second opsin gene expressed in the ultraviolet-sensitive R7
photoreceptor cells of Drosophila melanogaster.
NEUROSCIENCE 7(5) 1558-1566 (1987).

Documentation
Opsins, the light-absorbing molecules that mediate vision [1,2], are
integral membrane proteins that belong to a superfamily of G protein-coupled
receptors (GPCRs). The activating ligands of the different superfamily
members vary widely in structure and character, yet the proteins appear
faithfully to have conserved a basic structural framework, believed to
consist of 7 transmembrane (TM) helices. Although the sequences of these
proteins are very diverse, reflecting to some extent this broad range of
activating ligands, nevertheless, motifs have been identified in the TM
regions that are characteristic of virtually the entire superfamily [3,4].
Amongst the exceptions are the olfactory receptors, which cluster together
in a subfamily, which lacks significant matches with domains 2, 4 and 6.
Interestingly, the opsins also seem to be emerging as increasingly atypical
of the superfamily, clustering most strongly, in phylogenetic analyses, with
the olfactory receptors [4]. 
 
The visual pigments comprise an apoprotein (opsin), covalently linked to
the chromophore 11-cis-retinal. The covalent link is in the form of a
protonated Schiff base between the retinal and a lysine residue located in
TM domain 7. Vision is effected through the absorption of a photon by the
chromophore, which is isomerised to the all-trans form, promoting a
conformational change in the protein.
 
By contrast with vertebrate rhodopsin, which is found in rod cells, insect
photoreceptors are found in the ommatidia that comprise the compound eyes.
Each Drosophila eye has 800 ommatidia, each of which contains 8 photo-
receptor cells (designated R1-R8): R1-R6 are outer cells, while R7 and R8
are inner cells. Opsins RH3 and RH4 are sensitive to UV light [5-7].
 
OPSINRH3RH4 is a 7-element fingerprint that provides a signature for the
RH3 and RH4 opsins. The fingerprint was derived from an initial alignment
of 5 sequences: the motifs were drawn from conserved sections within either
loop or N- and C-terminal regions, focusing on those areas of the alignment
that characterise the RH3/RH4 opsins but distinguish them from the rest of
the rhodopsin-like superfamily - motifs 1 and 2 lie at the N-terminus;
motif 3 spans the first external loop; motif 4 lies in the second external
loop; motif 5 spans the C-terminal half of TM domain 5; motif 6 lies in the
the third cytoplasmic loop; and motif 7 lies at the C-terminus. A single
iteration on OWL28.1 was required to reach convergence, no further sequences
being identified beyond the starting set.  
 
An update on SPTR37_9f identified a true set of 8 sequences, and 3
partial matches.
Summary Information
   8 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
1 codes involving 4 elements
1 codes involving 3 elements
1 codes involving 2 elements
Composite Feature Index
78888888
60000000
50000000
41001011
31001001
20001001
1234567
True Positives
O61473        O61474        OPS3_DROME    OPS3_DROPS    
OPS4_DROME OPS4_DROPS OPS4_DROVI OPSV_APIME
True Positive Partials
Codes involving 4 elements
OPS2_SCHGR
Codes involving 3 elements
OPSB_APIME
Codes involving 2 elements
OPS5_DROME
Sequence Titles
O61473      SHORT WAVELENGTH-SENSITIVE OPSIN - CATAGLYPHIS BOMBYCINA (SAHARAN SILVER ANT). 
O61474 SHORT WAVELENGTH-SENSITIVE OPSIN - CAMPONOTUS ABDOMINALIS (CARPENTER ANT).
OPS3_DROME Opsin Rh3 (Inner R7 photoreceptor cells opsin) - Drosophila melanogaster (Fruit fly).
OPS3_DROPS Opsin Rh3 (Inner R7 photoreceptor cells opsin) - Drosophila pseudoobscura (Fruit fly).
OPS4_DROME Opsin Rh4 (Inner R7 photoreceptor cells opsin) - Drosophila melanogaster (Fruit fly).
OPS4_DROPS Opsin Rh4 (Inner R7 photoreceptor cells opsin) - Drosophila pseudoobscura (Fruit fly).
OPS4_DROVI Opsin Rh4 (Inner R7 photoreceptor cells opsin) - Drosophila virilis (Fruit fly).
OPSV_APIME OPSIN, ULTRAVIOLET-SENSITIVE (AMUVOP) (BUVOPS) - APIS MELLIFERA (HONEYBEE).

OPS2_SCHGR Opsin-2 - Schistocerca gregaria (Desert locust).

OPSB_APIME Opsin, blue-sensitive (AMBLOP) - Apis mellifera (Honeybee).

OPS5_DROME Opsin Rh5 - Drosophila melanogaster (Fruit fly).
Scan History
OWL28_1    1  100  NSINGLE    
SPTR37_9f 2 17 NSINGLE
Initial Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
DLQFLGWNVPPDQIQHI OPS4_DROVI 27 27 -
ETRLLGWNVPPEELRHI OPS3_DROME 27 27 -
ELQFLGWNVPPDQIQYI OPS4_DROPS 24 24 -
ESRLLGWNVPPDELRHI OPS3_DROPS 26 26 -
DLQFLGWNVPPDQIQYI OPS4_DROME 23 23 -

Motif 2 width=17
Element Seqn Id St Int Rpt
PEHWLTQLEPPASMHYM OPS4_DROVI 44 0 -
PEHWLTYPEPPESMNYL OPS3_DROME 44 0 -
PEHWLTQLEPPASMHYM OPS4_DROPS 41 0 -
PEHWLIYPEPPESMNYL OPS3_DROPS 43 0 -
PEHWLTQLEPPASMHYM OPS4_DROME 40 0 -

Motif 3 width=12
Element Seqn Id St Int Rpt
IYNSFHRGFALG OPS4_DROVI 115 54 -
IYNSFHQGYALG OPS3_DROME 115 54 -
IYNSFHRGFALG OPS4_DROPS 112 54 -
IYNSFHQGYALG OPS3_DROPS 114 54 -
IFNSFHRGFAIY OPS4_DROME 109 52 -

Motif 4 width=15
Element Seqn Id St Int Rpt
YLTDNFDTRLFVGTI OPS4_DROVI 211 84 -
YLTDNFDTRLFVACI OPS3_DROME 211 84 -
YLSDNFDTRLFVGTI OPS4_DROPS 208 84 -
YLTDNFDTRLFVACI OPS3_DROPS 210 84 -
YLSDNFDTRLFVGTI OPS4_DROME 207 86 -

Motif 5 width=18
Element Seqn Id St Int Rpt
TLMIIYYYSQIVGHVFSH OPS4_DROVI 234 8 -
TTMITYYYSQIVGHVFSH OPS3_DROME 234 8 -
TLMILYYYSQIVGHVFNH OPS4_DROPS 231 8 -
TTMITYYYSQIVGHVFSH OPS3_DROPS 233 8 -
TLMILYYYSQIVGHVFSH OPS4_DROME 230 8 -

Motif 6 width=15
Element Seqn Id St Int Rpt
SNVDKSKDTAEIRIA OPS4_DROVI 269 17 -
SNVDKNKETAEIRIA OPS3_DROME 269 17 -
SNVDKSKETAEIRIA OPS4_DROPS 266 17 -
SNVDKSKEAAEIRIA OPS3_DROPS 268 17 -
SNVDKSKETAEIRIA OPS4_DROME 265 17 -

Motif 7 width=19
Element Seqn Id St Int Rpt
RMELQKRCPWLAIDEKAPE OPS4_DROVI 346 62 -
RMELQKRCPWLALNEKAPE OPS3_DROME 346 62 -
RMELQKRCPWLGVNEKSGE OPS4_DROPS 343 62 -
RMELQKRCPWLAISEKAPE OPS3_DROPS 345 62 -
RLELQKRCPWLGVNEKSGE OPS4_DROME 342 62 -
Final Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
DLQFLGWNVPPDQIQHI OPS4_DROVI 27 27 -
ETRLLGWNVPPEELRHI OPS3_DROME 27 27 -
ELQFLGWNVPPDQIQYI OPS4_DROPS 24 24 -
ESRLLGWNVPPDELRHI OPS3_DROPS 26 26 -
DLQFLGWNVPPDQIQYI OPS4_DROME 23 23 -
PPRLLGWNVPAEELVHI O61474 17 17 -
PPRLLGWNVPAEELVHI O61473 18 18 -
PPRLLGWNVPAEELIHI OPSV_APIME 17 17 -

Motif 2 width=17
Element Seqn Id St Int Rpt
PEHWLTQLEPPASMHYM OPS4_DROVI 44 0 -
PEHWLTYPEPPESMNYL OPS3_DROME 44 0 -
PEHWLTQLEPPASMHYM OPS4_DROPS 41 0 -
PEHWLIYPEPPESMNYL OPS3_DROPS 43 0 -
PEHWLTQLEPPASMHYM OPS4_DROME 40 0 -
PEHXLVYPEPNPSLHYL O61474 34 0 -
PEHWLVYPEPNPSLHYL O61473 35 0 -
PEHWLVYPEPNPSLHYL OPSV_APIME 34 0 -

Motif 3 width=12
Element Seqn Id St Int Rpt
IYNSFHRGFALG OPS4_DROVI 115 54 -
IYNSFHQGYALG OPS3_DROME 115 54 -
IYNSFHRGFALG OPS4_DROPS 112 54 -
IYNSFHQGYALG OPS3_DROPS 114 54 -
IFNSFHRGFAIY OPS4_DROME 109 52 -
IYNSFHTGFATG O61474 105 54 -
IYNSFNTGFATG O61473 106 54 -
IYNSFNTGFALG OPSV_APIME 105 54 -

Motif 4 width=15
Element Seqn Id St Int Rpt
YLTDNFDTRLFVGTI OPS4_DROVI 211 84 -
YLTDNFDTRLFVACI OPS3_DROME 211 84 -
YLSDNFDTRLFVGTI OPS4_DROPS 208 84 -
YLTDNFDTRLFVACI OPS3_DROPS 210 84 -
YLSDNFDTRLFVGTI OPS4_DROME 207 86 -
YLTDSQEIRYFVPTI O61474 201 84 -
YLTDTPEIRYFVATI O61473 202 84 -
YLTDTNEIRIFVATI OPSV_APIME 201 84 -

Motif 5 width=18
Element Seqn Id St Int Rpt
TLMIIYYYSQIVGHVFSH OPS4_DROVI 234 8 -
TTMITYYYSQIVGHVFSH OPS3_DROME 234 8 -
TLMILYYYSQIVGHVFNH OPS4_DROPS 231 8 -
TTMITYYYSQIVGHVFSH OPS3_DROPS 233 8 -
TLMILYYYSQIVGHVFSH OPS4_DROME 230 8 -
MLLIIYYYSQIVGHVVSH O61474 224 8 -
MSLIIYYYSQIVSHVVNH O61473 225 8 -
MILIIYYYSQIVSHVVNH OPSV_APIME 224 8 -

Motif 6 width=15
Element Seqn Id St Int Rpt
SNVDKSKDTAEIRIA OPS4_DROVI 269 17 -
SNVDKNKETAEIRIA OPS3_DROME 269 17 -
SNVDKSKETAEIRIA OPS4_DROPS 266 17 -
SNVDKSKEAAEIRIA OPS3_DROPS 268 17 -
SNVDKSKETAEIRIA OPS4_DROME 265 17 -
SNVNTNAQSAEIRIA O61474 259 17 -
SNTNTNAQSAEIRIA O61473 260 17 -
SNANTSSQSAEIRIA OPSV_APIME 259 17 -

Motif 7 width=19
Element Seqn Id St Int Rpt
RMELQKRCPWLAIDEKAPE OPS4_DROVI 346 62 -
RMELQKRCPWLALNEKAPE OPS3_DROME 346 62 -
RMELQKRCPWLGVNEKSGE OPS4_DROPS 343 62 -
RMELQKRCPWLAISEKAPE OPS3_DROPS 345 62 -
RLELQKRCPWLGVNEKSGE OPS4_DROME 342 62 -
RLELQKRLPWLELQEKPVA O61474 336 62 -
RLELQKRLPWLELQEKPIE O61473 337 62 -
RLELQKRLPWLELQEKPIS OPSV_APIME 336 62 -