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PR00571

Identifier
ENDOTHELINBR  [View Relations]  [View Alignment]  
Accession
PR00571
No. of Motifs
8
Creation Date
10-SEP-1996  (UPDATE 07-JUN-1999)
Title
Endothelin-B receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR00366 ENDOTHELINR
INTERPRO; IPR001112
GCRDB; GCR_0237; GCR_0937; GCR_0068; GCR_0402; GCR_0474; GCR_0615

GCRDB; GCR_0701
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Endothelin.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, pp111-116.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Endothelins play an important role in the regulation of the cardiovascular
system [6]. They are the most potent vasoconstrictors identified, they
stimulate cardiac contraction, regulate release of vasoactive substances,
and stimulate mitogenesis in blood vessels in primary culture. They also
stimulate contraction in almost all other smooth muscles (e.g., uterus,
bronchus, vas deferens, stomach) and stimulate secretion in several tissues
(e.g., kidney, liver and adrenals) [6]. Endothelin receptors have also been
found in the brain (e.g., in the cerebral cortex, cerebellum and glial
cells). Endothelins have been implicated in a variety of pathophysiological
conditions associated with stress [6].
 
The ETB receptors are thought to play a significant role in endothelium-
dependent vasodilation and a lesser role in vasoconstriction [6]. In the CNS,
the receptor has been found in the cerebral cortex, hippocampus, cerebellum
and astrocytes. ETB receptors activate the phosphoinositide pathway through
a pertussis-toxin-insensitive G protein, though some actions are pertussis-
sensitive [6].
 
ENDOTHELINBR is an 8-element fingerprint that provides a signature for
the endothelin-B receptors. The fingerprint was derived from an initial
alignment of 5 sequences: the motifs were drawn from conserved sections   
within either loop or N- and C-terminal regions, focusing on those areas
of the alignment that characterise the endothelin-B receptors but
distinguish them from the rest of the rhodopsin-like superfamily - motifs
1-4 span the N-terminus; motifs 5 and 6 span the first and second external
loops res.; and motifs 7 and 8 lie at the C-terminus. A single iteration on 
OWL28.1 was required to reach convergence, no further sequences being
identified beyond the starting set.
 
An update on SPTR37_9f identified a true set of 6 sequences, and 1
partial match.
Summary Information
   6 codes involving  8 elements
0 codes involving 7 elements
1 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
866666666
700000000
611111100
500000000
400000000
300000000
200000000
12345678
True Positives
ETBR_BOVIN    ETBR_HUMAN    ETBR_MOUSE    ETBR_PIG      
ETBR_RAT O77508
True Positive Partials
Codes involving 6 elements
O15343
Sequence Titles
ETBR_BOVIN  ENDOTHELIN B RECEPTOR PRECURSOR (ET-B) (ENDOTHELIN RECEPTOR NON-SELECTIVE TYPE) - BOS TAURUS (BOVINE). 
ETBR_HUMAN ENDOTHELIN B RECEPTOR PRECURSOR (ET-B) (ENDOTHELIN RECEPTOR NON-SELECTIVE TYPE) - HOMO SAPIENS (HUMAN).
ETBR_MOUSE ENDOTHELIN B RECEPTOR PRECURSOR (ET-B) (ENDOTHELIN RECEPTOR NON-SELECTIVE TYPE) - MUS MUSCULUS (MOUSE).
ETBR_PIG ENDOTHELIN B RECEPTOR PRECURSOR (ET-B) (ENDOTHELIN RECEPTOR NON-SELECTIVE TYPE) - SUS SCROFA (PIG).
ETBR_RAT ENDOTHELIN B RECEPTOR PRECURSOR (ET-B) (ENDOTHELIN RECEPTOR NON-SELECTIVE TYPE) - RATTUS NORVEGICUS (RAT).
O77508 ENDOTHELIN-B RECEPTOR - EQUUS CABALLUS (HORSE).

O15343 ET-B RECEPTOR - HOMO SAPIENS (HUMAN).
Scan History
OWL28_1    1  50   NSINGLE    
SPTR37_9f 2 7 NSINGLE
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
QSSASRCGRALVALLLACG ETBR_RAT 2 2 -
QSPASRCGRALVALLLACG ETBR_MOUSE 2 2 -
QPPPSLCGRALVALVLACG ETBR_HUMAN 2 2 -
QPLRSLCGRALVALIFACG ETBR_PIG 2 2 -
QPLPSLCGRALVALILACG ETBR_BOVIN 2 2 -

Motif 2 width=18
Element Seqn Id St Int Rpt
GLLGVWGEKRGFPPAQAT ETBR_RAT 20 -1 -
GFLGVWGEKRGFPPAQAT ETBR_MOUSE 20 -1 -
GLSRIWGEERGFPPDRAT ETBR_HUMAN 20 -1 -
GVAGVQSEERGFPPAGAT ETBR_PIG 20 -1 -
GVAGIQAEEREFPPAGAT ETBR_BOVIN 20 -1 -

Motif 3 width=17
Element Seqn Id St Int Rpt
SLLGTKEVMTPPTKTSW ETBR_RAT 39 1 -
SLLGTKEVMTPPTKTSW ETBR_MOUSE 39 1 -
PLLQTAEIMTPPTKTLW ETBR_HUMAN 38 0 -
PALRTGEIVAPPTKTFW ETBR_PIG 39 1 -
PLPGTGEMMETPTETSW ETBR_BOVIN 39 1 -

Motif 4 width=21
Element Seqn Id St Int Rpt
RSSAPAEVTKGGRVAGVPPRS ETBR_RAT 65 9 -
RSSAPAEVTKGGRGAGVPPRS ETBR_MOUSE 65 9 -
RSLAPAEVPKGDRTAGSPPRT ETBR_HUMAN 64 9 -
RSSSPPQMPKGGRMAGPPART ETBR_PIG 65 9 -
RSSATPQIPRGGRMAGIPPRT ETBR_BOVIN 65 9 -

Motif 5 width=12
Element Seqn Id St Int Rpt
DWPFGAEMCKLV ETBR_RAT 166 80 -
DWPFGAEMCKLV ETBR_MOUSE 166 80 -
DWPFGAEMCKLV ETBR_HUMAN 166 81 -
DWPFGVEMCKLV ETBR_PIG 167 81 -
DWPFGVEMCKLV ETBR_BOVIN 165 79 -

Motif 6 width=15
Element Seqn Id St Int Rpt
NPFQKTAFMQFYKTA ETBR_RAT 258 80 -
NPFQKTAFMQFYKTA ETBR_MOUSE 258 80 -
HPVQKTAFMQFYKTA ETBR_HUMAN 258 80 -
HPTQKTAFMQFYKTA ETBR_PIG 259 80 -
HPTQKTAFMQFYKTA ETBR_BOVIN 257 80 -

Motif 7 width=19
Element Seqn Id St Int Rpt
FEEKQSLEEKQSCLKFKAN ETBR_RAT 408 135 -
FEEKQSLEEKQSCLKFKAN ETBR_MOUSE 408 135 -
FEEKQSLEEKQSCLKFKAN ETBR_HUMAN 408 135 -
FEEKQSLEEKQSCLKFKAN ETBR_PIG 409 135 -
FEEKQSLEEKQSCLKFKAN ETBR_BOVIN 407 135 -

Motif 8 width=15
Element Seqn Id St Int Rpt
DHGYDNFRSSNKYSS ETBR_RAT 427 0 -
DHGYDNFRSSNKYSS ETBR_MOUSE 427 0 -
DHGYDNFRSSNKYSS ETBR_HUMAN 427 0 -
DHGYDNFRSSNKYSS ETBR_PIG 428 0 -
DHGYDNFRSSNKYSS ETBR_BOVIN 426 0 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
QSSASRCGRALVALLLACG ETBR_RAT 2 2 -
QSPASRCGRALVALLLACG ETBR_MOUSE 2 2 -
QPPPSLCGRALVALVLACG ETBR_HUMAN 2 2 -
QPLRSLCGRALVALIFACG ETBR_PIG 2 2 -
QPLPSLCGRALVALILACG ETBR_BOVIN 2 2 -
QPLPTLCGRVLVALILACG O77508 2 2 -

Motif 2 width=18
Element Seqn Id St Int Rpt
GLLGVWGEKRGFPPAQAT ETBR_RAT 20 -1 -
GFLGVWGEKRGFPPAQAT ETBR_MOUSE 20 -1 -
GLSRIWGEERGFPPDRAT ETBR_HUMAN 20 -1 -
GVAGVQSEERGFPPAGAT ETBR_PIG 20 -1 -
GVAGIQAEEREFPPAGAT ETBR_BOVIN 20 -1 -
GVAGVQGEERRFPPARAT O77508 20 -1 -

Motif 3 width=17
Element Seqn Id St Int Rpt
SLLGTKEVMTPPTKTSW ETBR_RAT 39 1 -
SLLGTKEVMTPPTKTSW ETBR_MOUSE 39 1 -
PLLQTAEIMTPPTKTLW ETBR_HUMAN 38 0 -
PALRTGEIVAPPTKTFW ETBR_PIG 39 1 -
PLPGTGEMMETPTETSW ETBR_BOVIN 39 1 -
PLLGFEEIMTPPTKTSW O77508 39 1 -

Motif 4 width=21
Element Seqn Id St Int Rpt
RSSAPAEVTKGGRVAGVPPRS ETBR_RAT 65 9 -
RSSAPAEVTKGGRGAGVPPRS ETBR_MOUSE 65 9 -
RSLAPAEVPKGDRTAGSPPRT ETBR_HUMAN 64 9 -
RSSSPPQMPKGGRMAGPPART ETBR_PIG 65 9 -
RSSATPQIPRGGRMAGIPPRT ETBR_BOVIN 65 9 -
RLSAPPQMPKAGRTAGAQRRT O77508 65 9 -

Motif 5 width=12
Element Seqn Id St Int Rpt
DWPFGAEMCKLV ETBR_RAT 166 80 -
DWPFGAEMCKLV ETBR_MOUSE 166 80 -
DWPFGAEMCKLV ETBR_HUMAN 166 81 -
DWPFGVEMCKLV ETBR_PIG 167 81 -
DWPFGVEMCKLV ETBR_BOVIN 165 79 -
DWPFGVEMCKLV O77508 167 81 -

Motif 6 width=15
Element Seqn Id St Int Rpt
NPFQKTAFMQFYKTA ETBR_RAT 258 80 -
NPFQKTAFMQFYKTA ETBR_MOUSE 258 80 -
HPVQKTAFMQFYKTA ETBR_HUMAN 258 80 -
HPTQKTAFMQFYKTA ETBR_PIG 259 80 -
HPTQKTAFMQFYKTA ETBR_BOVIN 257 80 -
HPTQKTAFMQFYKNA O77508 259 80 -

Motif 7 width=19
Element Seqn Id St Int Rpt
FEEKQSLEEKQSCLKFKAN ETBR_RAT 408 135 -
FEEKQSLEEKQSCLKFKAN ETBR_MOUSE 408 135 -
FEEKQSLEEKQSCLKFKAN ETBR_HUMAN 408 135 -
FEEKQSLEEKQSCLKFKAN ETBR_PIG 409 135 -
FEEKQSLEEKQSCLKFKAN ETBR_BOVIN 407 135 -
FEEKQSLEDKQSCLKFKAN O77508 409 135 -

Motif 8 width=15
Element Seqn Id St Int Rpt
DHGYDNFRSSNKYSS ETBR_RAT 427 0 -
DHGYDNFRSSNKYSS ETBR_MOUSE 427 0 -
DHGYDNFRSSNKYSS ETBR_HUMAN 427 0 -
DHGYDNFRSSNKYSS ETBR_PIG 428 0 -
DHGYDNFRSSNKYSS ETBR_BOVIN 426 0 -
DHGYDNFRSSNKYSS O77508 428 0 -