SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR00566

Identifier
DOPAMINED1BR  [View Relations]  [View Alignment]  
Accession
PR00566
No. of Motifs
5
Creation Date
17-AUG-1996  (UPDATE 07-JUN-1999)
Title
Dopamine 1B receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR00242 DOPAMINER
INTERPRO; IPR000497
GCRDB; GCR_0072; GCR_0169; GCR_0131
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. GRANDY, D.K., MARCHIONNI, M.A., MAKAM, H., STOFKO, R.E., ALFANO, M.,
FROTHINGHAM, L., FISCHER, J.B., BURKE-HOWIE, K.J., BUNZOW, J.R.,
SERVER, A.C. AND CIVELLI, O.
Cloning of the cDNA and gene for a human D2 dopamine receptor. 
PROC.NATL.ACAD.SCI.U.S.A. 86 9762-9766 (1989).
 
7. WATSON, S. AND ARKINSTALL, S.
Dopamine.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, pp96-110.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Dopamine neurons in the vertebrate central nervous system are involved in
the initiation and execution of movement, the maintenance of emotional
stability, and the regulation of pituitary function [6]. Various human
neurological diseases (e.g., Parkinson disease and schizophrenia), are
believed to be manifestations of dopamine and dopamine receptor imbalance.
The receptors have been divided into several different subtypes (designated
D1-D5), which may be distinguished by their G protein coupling, ligand
specificity, anatomical distribution and physiological effects.
 
D1 receptors are found in greatest abundance in the caudate-putamen,
nucleus accumbens and olfactory tubercle, with lower levels in the frontal
cortex, habenula, amygdala, hypothalamus and thalamus [7]. In the periphery,
binding sites are found in the kidney, heart, liver and parathyroid gland
[7]. The receptors stimulate adenylyl cyclase through Gs; they may also be
able to stimulate phosphoinositide metabolism [7]. 
 
DOPAMINED1BR is a 5-element fingerprint that provides a signature for the
D1B family of dopamine receptors. The fingerprint was derived from an
initial alignment of 4 sequences: the motifs were drawn from conserved
sections within either loop or N- and C-terminal regions, focusing on
those areas of the alignment that characterise the D1B receptors but
distinguish them from the rest of the rhodopsin-like superfamily - motif 1
lies at the N-terminus leading into the N-terminal portion of TM domain 1;
motif 2 lies in the third cytoplasmic loop; motif 3 lies in the third
external loop; and motifs 4 and 5 span the C-terminus. A single iteration
on OWL28.1 was required to reach convergence, no further sequences being
identified beyond the starting set. 
 
An update on SPTR37_9f identified a true set of 4 sequences.
Summary Information
4 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
544444
400000
300000
200000
12345
True Positives
DBDR_HUMAN    DBDR_RAT      DBDR_XENLA    Q98843        
Sequence Titles
DBDR_HUMAN  D(1B) DOPAMINE RECEPTOR (D(5) DOPAMINE RECEPTOR) - HOMO SAPIENS (HUMAN). 
DBDR_RAT D(1B) DOPAMINE RECEPTOR (D(5) DOPAMINE RECEPTOR) - RATTUS NORVEGICUS (RAT).
DBDR_XENLA D(1B) DOPAMINE RECEPTOR (D(5) DOPAMINE RECEPTOR) - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
Q98843 DOPAMINE D1B RECEPTOR - ANGUILLA ANGUILLA (EUROPEAN FRESHWATER EEL).
Scan History
OWL28_1    1  100  NSINGLE    
SPTR37_9f 2 4 NSINGLE
Initial Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
QIVTGSLLLLLIFW DBDR_XENLA 41 41 -
QVVTACLLTLLIIW DBDR_HUMAN 40 40 -
QVAAGSLLALLILW B55886 29 29 -
QVVTAGLLTLLIVW DBDR_RAT 38 38 -

Motif 2 width=10
Element Seqn Id St Int Rpt
AEHAQSCRSR DBDR_RAT 260 208 -
AEHAQSCRCN B55886 284 241 -
AEHAQSCRSS DBDR_HUMAN 266 212 -
AEHAQSCRSN DBDR_XENLA 248 193 -

Motif 3 width=11
Element Seqn Id St Int Rpt
PGHPQAGLPCV DBDR_XENLA 312 54 -
AEGPKTGFPCV DBDR_RAT 321 51 -
PSDPRAGLPCV B55886 345 51 -
PEGPPAGFPCV DBDR_HUMAN 326 50 -

Motif 4 width=9
Element Seqn Id St Int Rpt
TPVETVNIS B55886 401 45 -
TPVETVNIS DBDR_HUMAN 382 45 -
TPVETVNIS DBDR_XENLA 368 45 -
TPVQTVNIS DBDR_RAT 377 45 -

Motif 5 width=17
Element Seqn Id St Int Rpt
FHKDIVTAYVNMIPNVV DBDR_XENLA 388 11 -
FHKEIATAYVHMIPNAV DBDR_RAT 397 11 -
FHKEIAAAYIHMMPNAV DBDR_HUMAN 402 11 -
FHKEIVTAYVNMIPNVV B55886 420 10 -
Final Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
QVVTACLLTLLIIW DBDR_HUMAN 40 40 -
QIVTGSLLLLLIFW DBDR_XENLA 41 41 -
QVVTAGLLTLLIVW DBDR_RAT 38 38 -
RTVTGCLLSLLILW Q98843 43 43 -

Motif 2 width=10
Element Seqn Id St Int Rpt
AEHAQSCRSS DBDR_HUMAN 266 212 -
AEHAQSCRSN DBDR_XENLA 248 193 -
AEHAQSCRSR DBDR_RAT 260 208 -
AEHAQSCRTN Q98843 254 197 -

Motif 3 width=11
Element Seqn Id St Int Rpt
PEGPPAGFPCV DBDR_HUMAN 326 50 -
PGHPQAGLPCV DBDR_XENLA 312 54 -
AEGPKTGFPCV DBDR_RAT 321 51 -
PTDHTAGLPCV Q98843 315 51 -

Motif 4 width=9
Element Seqn Id St Int Rpt
TPVETVNIS DBDR_HUMAN 382 45 -
TPVETVNIS DBDR_XENLA 368 45 -
TPVQTVNIS DBDR_RAT 377 45 -
TPVETVNIS Q98843 371 45 -

Motif 5 width=17
Element Seqn Id St Int Rpt
FHKEIAAAYIHMMPNAV DBDR_HUMAN 402 11 -
FHKDIVTAYVNMIPNVV DBDR_XENLA 388 11 -
FHKEIATAYVHMIPNAV DBDR_RAT 397 11 -
FHKEIVTAYVNMIPNVV Q98843 391 11 -