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PR00550

Identifier
HYPRGLYCEMIC  [View Relations]  [View Alignment]  
Accession
PR00550
No. of Motifs
3
Creation Date
22-MAY-1996  (UPDATE 27-JUN-1999)
Title
Hyperglycemic hormone signature
Database References
PRINTS; PR00548 HYPRGLYCEMC1; PR00549 HYPRGLYCEMC2
PROSITE; PS01250 CHH_MIH_GIH
PFAM; PF01147 Crust_neurohorm
INTERPRO; IPR001166
Literature References
1. KEGEL, G., REICHWEIN, B., WEESE, S., GAUS, G., PETER-KATALINIC, J. 
AND KELLER, R.
Amino acid sequence of the crustacean hyperglycemic hormone (CHH) from
the shore crab, Carcinus maenas. 
FEBS LETT. 255 10-14 (1989).
 
2. MARTIN, G., SOROKINE, O. AND VAN DORSSELAER, A.
Isolation and molecular characterization of a hyperglycemic neuropeptide
from the sinus gland of the terrestrial isopod Armadillidium vulgare
EUR.J.BIOCHEMISTRY 211 601-607 (1993).
 
3. CHANG, E.S., PRESTWICH, G.D. AND BRUCE, M.J.
Amino acid sequence of a peptide with both molt-inhibiting and
hyperglycemic activities in the lobster, Homarus.
BIOCHEM.BIOPHYS.RES.COMMUN. 171 818-826 (1990).

Documentation
Hyperglycemic hormone, which controls blood sugar levels, is an abundant
peptide in the sinus glands of isopods and decapods [1,2]. The peptide is a
potent secretagogue, releasing digestive enzymes from the hepatopancreas.
It may act as a stress hormone. The peptide contains around 70 amino acid
residues, and includes 6 cysteine residues involved in disulphide bond
formation [2]. The sequences show a high degree of similarity to the
American lobster molt-inhibiting hormone [3], which also shows hyper-
glycemic hormone activity.
 
Sequence analysis reveals at least 2 discrete sequence families,
characterised by distinctive N- and C-terminal signatures: broadly, the
families represent separate groups of the crustacean hyperglycemic-like
hormones and the molt-inhibiting-like hormones. The positions of 5 of the
6 cysteine residues are conserved between these families, the first Cys
being displaced by one residue position.
 
HYPRGLYCEMIC is a 3-element fingerprint that provides a signature for the
hyperglycemic hormone family. The fingerprint was derived from an initial
alignment of 7 sequences: the motifs were drawn from conserved regions
spanning the central portion of the alignment and encode 5 of 6 conserved
cysteine residues involved in disulphide bond formation. Two iterations on
OWL27.1 were required to reach convergence, at which point a true set
comprising 14 sequences was identified.
 
An update on SPTR37_9f identified a true set of 27 sequences.
Summary Information
27 codes involving  3 elements
0 codes involving 2 elements
Composite Feature Index
3272727
2000
123
True Positives
CHH1_MACRS    CHH1_ORCLI    CHH2_ORCLI    CHHA_HOMAM    
CHHB_HOMAM CHH_ARMVU CHH_CANPG CHH_CARMA
CHH_PENJP CHH_PROBO GIH_HOMAM MIH_CALSI
MIH_CANPG MIH_CARMA MIH_PENJP MIH_PROCL
MOH1_CANPG MOH2_CANPG O02379 O15980
O15981 O15982 O61389 O76534
O77220 Q26491 Q26492
Sequence Titles
CHH1_MACRS  CRUSTACEAN HYPERGLYCEMIC HORMONE ISOFORM I (CHH I) (MAR-CHH-I) - MACROBRACHIUM ROSENBERGII (GIANT FRESH WATER PRAWN). 
CHH1_ORCLI CRUSTACEAN HYPERGLYCEMIC HORMONE A PRECURSOR (CHH A) - ORCONECTES LIMOSUS (SPINYCHEEK CRAYFISH).
CHH2_ORCLI CRUSTACEAN HYPERGLYCEMIC HORMONE A* PRECURSOR (CHH A*) - ORCONECTES LIMOSUS (SPINYCHEEK CRAYFISH).
CHHA_HOMAM CRUSTACEAN HYPERGLYCEMIC HORMONE ISOFORM A PRECURSOR (CHH-A) (MOLT- INHIBITING HORMONE) (MIH) - HOMARUS AMERICANUS (AMERICAN LOBSTER).
CHHB_HOMAM CRUSTACEAN HYPERGLYCEMIC HORMONE ISOFORM B PRECURSOR (CHH-B) - HOMARUS AMERICANUS (AMERICAN LOBSTER).
CHH_ARMVU CRUSTACEAN HYPERGLYCEMIC HORMONE (CHH) - ARMADILLIDIUM VULGARE (WOODLICE) (PILLBUGS).
CHH_CANPG CRUSTACEAN HYPERGLYCEMIC HORMONE (CHH) - CANCER PAGURUS (ROCK CRAB).
CHH_CARMA CRUSTACEAN HYPERGLYCEMIC HORMONE PRECURSOR (CHH) - CARCINUS MAENAS (COMMON SHORE CRAB) (GREEN CRAB).
CHH_PENJP CRUSTACEAN HYPOGLYCEMIC HORMONE 3 PRECURSOR (CHH) (PEN-SGP-III) - PENAEUS JAPONICUS (KURUMA PRAWN).
CHH_PROBO CRUSTACEAN HYPERGLYCEMIC HORMONE (CHH) - PROCAMBARUS BOUVIERI (MEXICAN CRAYFISH).
GIH_HOMAM GONAD-INHIBITING HORMONE PRECURSOR (GIH) (VITELLOGENESIS-INHIBITING HORMONE) (VIH) - HOMARUS AMERICANUS (AMERICAN LOBSTER).
MIH_CALSI MOLT-INHIBITING HORMONE PRECURSOR (MIH) - CALLINECTES SAPIDUS (BLUE CRAB).
MIH_CANPG MOLT-INHIBITING HORMONE (MIH) - CANCER PAGURUS (ROCK CRAB).
MIH_CARMA MOLT-INHIBITING HORMONE PRECURSOR (MIH) - CARCINUS MAENAS (COMMON SHORE CRAB) (GREEN CRAB).
MIH_PENJP MOLT-INHIBITING HORMONE PRECURSOR (MIH) (PEJ-SGP-IV) - PENAEUS JAPONICUS (KURUMA PRAWN).
MIH_PROCL MOLT-INHIBITING HORMONE (MIH) - PROCAMBARUS CLARKII (RED SWAMP CRAYFISH).
MOH1_CANPG MANDIBULAR ORGAN-INHIBITING HORMONE-1 (MOIH-1) - CANCER PAGURUS (ROCK CRAB).
MOH2_CANPG MANDIBULAR ORGAN-INHIBITING HORMONE-2 (MOIH-2) - CANCER PAGURUS (ROCK CRAB).
O02379 MOLT-INHIBITING HORMONE PRECURSOR (MIH) (PEJ-SGP-IV) - PENAEUS JAPONICUS (KURUMA PRAWN).
O15980 CRUSTACEAN HYPERGLYCEMIC HORMONE 1 PRECURSOR (CHH) (PEJ-SGP-I) - PENAEUS JAPONICUS (KURUMA PRAWN).
O15981 CRUSTACEAN HYPERGLYCEMIC 5 HORMONE PRECURSOR (PEJ-SGP-V) - PENAEUS JAPONICUS (KURUMA PRAWN).
O15982 CRUSTACEAN HYPERGLYCEMIC HORMONE 7 PRECURSOR (PEJ-SGP-VII) - PENAEUS JAPONICUS (KURUMA PRAWN).
O61389 MOLT-INHIBITING HORMONE PRECURSOR (MIH) - CANCER MAGISTER (DUNGENESS CRAB).
O76534 PROBABLE MOLT-INHIBITING HORMONE PRECURSOR (MEE-MIH) - METAPENAEUS ENSIS (GREASYBACK SHRIMP) (SAND SHRIMP).
O77220 CRUSTACEAN HYPERGLYCEMIC HORMONE PRECURSOR (CHH) - MACROBRACHIUM LANCHESTERI (PRAWN).
Q26491 ION TRANSPORT PEPTIDE PRECURSOR (ITP) - SCHISTOCERCA GREGARIA (DESERT LOCUST).
Q26492 ION TRANSPORT PEPTIDE-LIKE PRECURSOR - SCHISTOCERCA GREGARIA (DESERT LOCUST).
Scan History
OWL27_1    2  100  NSINGLE    
SPTR37_9f 3 100 NSINGLE
Initial Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
DRNLFKKLNRVCEDCYN S52118 72 72 -
NRDLYKKVEWICEDCSN S39031 48 48 -
NRDLYEKVAWVCNDCAN A45586 15 15 -
DRNLFKKLDRVCEDCYN MIH_HOMAM 12 12 -
NRDLYKKVEWICDDCAN JC4143 48 48 -
DRALFNDLEHVCDDCYN CHH_CARMA 78 78 -
DRNLFKKLNRVCEDCYN S17651 12 12 -

Motif 2 width=16
Element Seqn Id St Int Rpt
NIFRNNDVGVMCKKDC A45586 31 -1 -
NLYRKPFVATTCRENC MIH_HOMAM 28 -1 -
NLYRKPFIVTTCRENC S17651 28 -1 -
NLYRKPFIVTTCRENC S52118 88 -1 -
NIFRKTGMASLCRRNC S39031 64 -1 -
NIYRSTGMASLCRKDC JC4143 64 -1 -
NLYRTSYVASACRSNC CHH_CARMA 94 -1 -

Motif 3 width=11
Element Seqn Id St Int Rpt
CYSNRVFRQCL S52118 103 -1 -
CFFNEDFVWCV S39031 79 -1 -
CFHTMWFLWCV A45586 46 -1 -
CFFNEDFLWCV JC4143 79 -1 -
CYSNWVFRQCL MIH_HOMAM 43 -1 -
CYSNLVFRQCM CHH_CARMA 109 -1 -
CYSNRVFRQCL S17651 43 -1 -
Final Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
DRAIFKKLDRVCEDCYN CHH_PROBO 12 12 -
DRAIFKKLDRVCEDCYN CHH2_ORCLI 73 73 -
DRAIFKKLDRVCEDCYN CHH1_ORCLI 73 73 -
DRNLFKKLDRVCEDCYN CHHA_HOMAM 72 72 -
DRELFKKLDRVCDDCYN O77220 73 73 -
DRELFKKLDRVCDDCYN CHH1_MACRS 12 12 -
DRNLFKKLNRVCEDCYN CHHB_HOMAM 72 72 -
DRVLLGKLNRLCDDCYN O15981 63 63 -
NRDLYKKVEWICEDCSN MIH_CANPG 13 13 -
DRQLLRRLGRVCDDCFN O15980 58 58 -
DRQLLRKLGRLCDDCYN CHH_PENJP 55 55 -
DRALFNDLEHVCDDCYN CHH_CARMA 78 78 -
NRDLYKKVEWICEDCSN MIH_CARMA 48 48 -
NRDLYKRVEWICEDCSN O61389 48 48 -
DRELLGRLSRLCDDCYN O15982 60 60 -
DRGLFSDLEHVCDDCYN CHH_CANPG 12 12 -
NRDIYKKVVRVCEDCTN O02379 41 41 -
NRDIYKKVVRVCEDCTN O76534 41 41 -
NRDLYKKVEWICDDCAN MIH_CALSI 48 48 -
NRDYNKKVVRVCEDCTN MIH_PENJP 13 13 -
DRGLFAQLDRVCEDCYN CHH_ARMVU 12 12 -
NRAMYEKVDWICKDCAN MOH1_CANPG 13 13 -
NRAMYEKVDWICKDCAN MOH2_CANPG 13 13 -
DKSIFARLDRICEDCYN Q26491 67 67 -
NRAVHGKVTRVCEDCYN MIH_PROCL 13 13 -
DKSIFARLDRICEDCYN Q26492 67 67 -
NRDLYEKVAWVCNDCAN GIH_HOMAM 47 47 -

Motif 2 width=16
Element Seqn Id St Int Rpt
NLYRKPYVATTCRQNC CHH_PROBO 28 -1 -
NLYRKPYVATTCRQNC CHH2_ORCLI 89 -1 -
NLYRKPYVATTCRQNC CHH1_ORCLI 89 -1 -
NLYRKPFVATTCRENC CHHA_HOMAM 88 -1 -
NLYRKPYVAIDCREGC O77220 89 -1 -
NLYRKPYVAIDCRRGC CHH1_MACRS 28 -1 -
NLYRKPFIVTTCRENC CHHB_HOMAM 88 -1 -
NVFREPNVATECRSNC O15981 79 -1 -
NIFRNTGMATLCRKNC MIH_CANPG 29 -1 -
NVFREPNVATECRSNC O15980 74 -1 -
NVFREPKVATGCRSNC CHH_PENJP 71 -1 -
NLYRTSYVASACRSNC CHH_CARMA 94 -1 -
NIFRKTGMASLCRRNC MIH_CARMA 64 -1 -
NIFRNTGMATLCRKNC O61389 64 -1 -
NVFREPKVAMECRSNC O15982 76 -1 -
NLYRNSYVASACRSNC CHH_CANPG 28 -1 -
NIFRLPGLDGMCRNRC O02379 57 -1 -
NIFRLPGLDGMCRDRC O76534 57 -1 -
NIYRSTGMASLCRKDC MIH_CALSI 64 -1 -
NIFRLPGLDGMCRNRC MIH_PENJP 29 -1 -
NLYRKPHVAAECRRDC CHH_ARMVU 28 -1 -
NIFRKDGLLNNCRSNC MOH1_CANPG 29 -1 -
NIFRQDGLLNNCRSNC MOH2_CANPG 29 -1 -
NLFREPQLHSLCRSDC Q26491 83 -1 -
NVFRDTDVLAGCRKGC MIH_PROCL 29 -1 -
NLFREPQLHSLCRKDC Q26492 83 -1 -
NIFRNNDVGVMCKKDC GIH_HOMAM 63 -1 -

Motif 3 width=11
Element Seqn Id St Int Rpt
CYANSVFRQCL CHH_PROBO 43 -1 -
CYANSVFRQCL CHH2_ORCLI 104 -1 -
CYANSVFRQCL CHH1_ORCLI 104 -1 -
CYSNWVFRQCL CHHA_HOMAM 103 -1 -
CYQNLVFRQCI O77220 104 -1 -
CYQNLVFRQCI CHH1_MACRS 43 -1 -
CYSNRVFRQCL CHHB_HOMAM 103 -1 -
CFYNLAFVQCL O15981 94 -1 -
CFFNEDFLWCV MIH_CANPG 44 -1 -
CYNNPVFRQCM O15980 89 -1 -
CYHNLIFLDCL CHH_PENJP 86 -1 -
CYSNLVFRQCM CHH_CARMA 109 -1 -
CFFNEDFVWCV MIH_CARMA 79 -1 -
CFFNEDFLWCV O61389 79 -1 -
CFFNPAFVQCL O15982 91 -1 -
CYSNVVFRQCM CHH_CANPG 43 -1 -
CFYNEWFLICL O02379 72 -1 -
CFNNEWFLVCL O76534 72 -1 -
CFFNEDFLWCV MIH_CALSI 79 -1 -
CFYNEWFLICL MIH_PENJP 44 -1 -
CYTTEVFESCL CHH_ARMVU 43 -1 -
CFYNTEFLWCI MOH1_CANPG 44 -1 -
CFYNTEFLWCI MOH2_CANPG 44 -1 -
CFKSPYFKGCL Q26491 98 -1 -
CFSSEMFKLCL MIH_PROCL 44 -1 -
CFTSDYFKGCI Q26492 98 -1 -
CFHTMDFLWCV GIH_HOMAM 78 -1 -