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PR00548

Identifier
HYPRGLYCEMC1  [View Relations]  [View Alignment]  
Accession
PR00548
No. of Motifs
4
Creation Date
22-MAY-1996  (UPDATE 07-JUN-1999)
Title
Hyperglycemic hormone type 1 signature
Database References
PRINTS; PR00550 HYPRGLYCEMIC
INTERPRO; IPR000346
Literature References
1. KEGEL, G., REICHWEIN, B., WEESE, S., GAUS, G., PETER-KATALINIC, J. 
AND KELLER, R.
Amino acid sequence of the crustacean hyperglycemic hormone (CHH) from
the shore crab, Carcinus maenas. 
FEBS LETT. 255 10-14 (1989).
 
2. MARTIN, G., SOROKINE, O. AND VAN DORSSELAER, A.
Isolation and molecular characterization of a hyperglycemic neuropeptide
from the sinus gland of the terrestrial isopod Armadillidium vulgare
EUR.J.BIOCHEMISTRY 211 601-607 (1993).
 
3. CHANG, E.S., PRESTWICH, G.D. AND BRUCE, M.J.
Amino acid sequence of a peptide with both molt-inhibiting and
hyperglycemic activities in the lobster, Homarus.
BIOCHEM.BIOPHYS.RES.COMMUN. 171 818-826 (1990).

Documentation
Hyperglycemic hormone, which controls blood sugar levels, is an abundant
peptide in the sinus glands of isopods and decapods [1,2]. The peptide is a
potent secretagogue, releasing digestive enzymes from the hepatopancreas.
It may act as a stress hormone. The peptide contains around 70 amino acid
residues, and includes 6 cysteine residues involved in disulphide bond
formation [2]. The sequences show a high degree of similarity to the
American lobster molt-inhibiting hormone [3], which also shows hyper-
glycemic hormone activity.
 
Sequence analysis reveals at least 2 discrete sequence families,
characterised by distinctive N- and C-terminal signatures: broadly, the
families represent separate groups of the crustacean hyperglycemic-like
hormones and the molt-inhibiting-like hormones. The positions of 5 of the
6 cysteine residues are conserved between these families, the first Cys
being displaced by one residue position.
 
HYPRGLYCEMC1 is a 4-element fingerprint that provides a signature for the
hyperglycemic-like hormones (type 1). The fingerprint was derived from an
initial alignment of 5 sequences: motif 1 was drawn from the conserved
N-terminal region of the alignment; and motifs 2-4 were drawn from the
C-terminal portion. Two iterations on OWL27.1 were required to reach
convergence, at which point a true set comprising 8 sequences was
identified, including the lobster molt-inhibiting hormone MIH_HOMAM. Two
partial matches were also found, both of which are hyperglycemic hormones
that fail to match motif 4.
 
An update on SPTR37_9f identified a true set of 9 sequences, and 1
partial match.
Summary Information
   9 codes involving  4 elements
0 codes involving 3 elements
1 codes involving 2 elements
Composite Feature Index
49999
30000
21100
1234
True Positives
CHH1_MACRS    CHH1_ORCLI    CHH2_ORCLI    CHHA_HOMAM    
CHHB_HOMAM CHH_CANPG CHH_CARMA CHH_PROBO
O77220
True Positive Partials
Codes involving 2 elements
CHH_ARMVU
Sequence Titles
CHH1_MACRS  CRUSTACEAN HYPERGLYCEMIC HORMONE ISOFORM I (CHH I) (MAR-CHH-I) - MACROBRACHIUM ROSENBERGII (GIANT FRESH WATER PRAWN). 
CHH1_ORCLI CRUSTACEAN HYPERGLYCEMIC HORMONE A PRECURSOR (CHH A) - ORCONECTES LIMOSUS (SPINYCHEEK CRAYFISH).
CHH2_ORCLI CRUSTACEAN HYPERGLYCEMIC HORMONE A* PRECURSOR (CHH A*) - ORCONECTES LIMOSUS (SPINYCHEEK CRAYFISH).
CHHA_HOMAM CRUSTACEAN HYPERGLYCEMIC HORMONE ISOFORM A PRECURSOR (CHH-A) (MOLT- INHIBITING HORMONE) (MIH) - HOMARUS AMERICANUS (AMERICAN LOBSTER).
CHHB_HOMAM CRUSTACEAN HYPERGLYCEMIC HORMONE ISOFORM B PRECURSOR (CHH-B) - HOMARUS AMERICANUS (AMERICAN LOBSTER).
CHH_CANPG CRUSTACEAN HYPERGLYCEMIC HORMONE (CHH) - CANCER PAGURUS (ROCK CRAB).
CHH_CARMA CRUSTACEAN HYPERGLYCEMIC HORMONE PRECURSOR (CHH) - CARCINUS MAENAS (COMMON SHORE CRAB) (GREEN CRAB).
CHH_PROBO CRUSTACEAN HYPERGLYCEMIC HORMONE (CHH) - PROCAMBARUS BOUVIERI (MEXICAN CRAYFISH).
O77220 CRUSTACEAN HYPERGLYCEMIC HORMONE PRECURSOR (CHH) - MACROBRACHIUM LANCHESTERI (PRAWN).

CHH_ARMVU CRUSTACEAN HYPERGLYCEMIC HORMONE (CHH) - ARMADILLIDIUM VULGARE (WOODLICE) (PILLBUGS).
Scan History
OWL27_1    2  100  NSINGLE    
SPTR37_9f 2 10 NSINGLE
Initial Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
VFDQACKGVYDRNLF MIH_HOMAM 2 2 -
VFDQACKGIYDRAIF S48141 63 63 -
IYDTSCKGVYDRALF CHH_CARMA 68 68 -
VFDQACKGVYDRNLF S17651 2 2 -
VFDQACKGVYDRNLF S52117 62 62 -

Motif 2 width=11
Element Seqn Id St Int Rpt
LYRKPFIVTTC S17651 29 12 -
LYRKPFVATTC S52117 89 12 -
LYRTSYVASAC CHH_CARMA 95 12 -
LYRKPYVATTC S48141 90 12 -
LYRKPFVATTC MIH_HOMAM 29 12 -

Motif 3 width=13
Element Seqn Id St Int Rpt
YSNWVFRQCLDDL S52117 104 4 -
YSNWVFRQCLDDL MIH_HOMAM 44 4 -
YSNRVFRQCLDDL S17651 44 4 -
YANSVFRQCLDDL S48141 105 4 -
YSNLVFRQCMDDL CHH_CARMA 110 4 -

Motif 4 width=15
Element Seqn Id St Int Rpt
LLLIDVLDEYISGVQ S48141 117 -1 -
LLMIDVIDEYVSNVQ S17651 56 -1 -
LLLSNVIDEYVSNVQ MIH_HOMAM 56 -1 -
LLMMDEFDQYARKVQ CHH_CARMA 122 -1 -
LLLSDVIDEYVSNVQ S52117 116 -1 -
Final Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
VFDQACKGIYDRAIF CHH1_ORCLI 63 63 -
VFDQACKGIYDRAIF CHH2_ORCLI 63 63 -
VFDQACKGIYDRAIF CHH_PROBO 2 2 -
VFDQACKGVYDRNLF CHHA_HOMAM 62 62 -
VFDQACKGVYDRNLF CHHB_HOMAM 62 62 -
ILDQSCKGIFDRELF CHH1_MACRS 2 2 -
ILDQSCKGIFDRELF O77220 63 63 -
IYDTSCKGVYDRALF CHH_CARMA 68 68 -
IYDTSCKGVYDRGLF CHH_CANPG 2 2 -

Motif 2 width=11
Element Seqn Id St Int Rpt
LYRKPYVATTC CHH1_ORCLI 90 12 -
LYRKPYVATTC CHH2_ORCLI 90 12 -
LYRKPYVATTC CHH_PROBO 29 12 -
LYRKPFVATTC CHHA_HOMAM 89 12 -
LYRKPFIVTTC CHHB_HOMAM 89 12 -
LYRKPYVAIDC CHH1_MACRS 29 12 -
LYRKPYVAIDC O77220 90 12 -
LYRTSYVASAC CHH_CARMA 95 12 -
LYRNSYVASAC CHH_CANPG 29 12 -

Motif 3 width=13
Element Seqn Id St Int Rpt
YANSVFRQCLDDL CHH1_ORCLI 105 4 -
YANSVFRQCLDDL CHH2_ORCLI 105 4 -
YANSVFRQCLDDL CHH_PROBO 44 4 -
YSNWVFRQCLDDL CHHA_HOMAM 104 4 -
YSNRVFRQCLDDL CHHB_HOMAM 104 4 -
YQNLVFRQCIQDL CHH1_MACRS 44 4 -
YQNLVFRQCIQDL O77220 105 4 -
YSNLVFRQCMDDL CHH_CARMA 110 4 -
YSNVVFRQCMEEL CHH_CANPG 44 4 -

Motif 4 width=15
Element Seqn Id St Int Rpt
LLLIDVLDEYISGVQ CHH1_ORCLI 117 -1 -
LLLIDVLDEYISGVQ CHH2_ORCLI 117 -1 -
LLLIDVVDEYISGVQ CHH_PROBO 56 -1 -
LLLSDVIDEYVSNVQ CHHA_HOMAM 116 -1 -
LLLSDVIDEYVSNVQ CHHB_HOMAM 116 -1 -
LQLMDDLDEYANAVQ CHH1_MACRS 56 -1 -
LQLMDQLDEYANAVQ O77220 117 -1 -
LLMMDEFDQYARKVQ CHH_CARMA 122 -1 -
LLLMDEFDKYARAVQ CHH_CANPG 56 -1 -