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PR00547

Identifier
XOPIOIDR  [View Relations]  [View Alignment]  
Accession
PR00547
No. of Motifs
7
Creation Date
19-MAY-1996  (UPDATE 07-JUN-1999)
Title
X opioid receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR00384 OPIOIDR
INTERPRO; IPR001420
GCRDB; GCR_0987
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Opioid peptides.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.205-213.
 
7. MOLLEREAU, C., PARMENTIER, M., MAILLEUX, P., BUTOUR, J.L., MOISAND, C.,
CHALON, P.L., CAPUT, D., VASSART, G. AND MEUNIER, J.C.
ORL1, a novel member of the opioid receptor family. Cloning, functional
expression and localization.
FEBS LETT. 341 33-38 (1994).

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
The term opioid refers to a class of substance that produces its effects
via the major classes of opioid receptor, termed mu, delta and kappa [6].
The receptors are found in the CNS and certain smooth muscles: mu-opioid
receptors are believed to mediate analgesia, hypothermia, respiratory
depression, miosis, bradycardia, nausea, euphoria and physical dependence,
beta-endorphin being the most potent endogenous ligand; delta-receptors
mediate analgesia; and kappa-opioid receptors are believed to mediate
analgesia, sedation, miosis and diuresis, dynorphin being the most potent
endogenous ligand.
 
The X-receptor is closely related to opioid receptors, on grounds of both
sequence and function, although it is not a typical opioid receptor [7].
X-receptors are found in many regions of the brain and spinal cord,
particularly limbic and hypothalamic structures. They are believed to
represent a new class of opioid receptors, with a potential role in
modulating various brain functions, including instinctive behaviours and
emotions [7].
 
XOPIOIDR is a 7-element fingerprint that provides a signature for the 
X opioid receptors. The fingerprint was derived from an initial alignment
of 3 sequences: the motifs were drawn from conserved sections within either
loop or N- and C-terminal regions, focusing on those areas of the alignment
that characterise the X opioid receptors but distinguish them from the rest
of the opioid receptor family - motifs 1 and 2 lie at the N-terminus; motif
3 spans the C-terminus of TM domain 2 leading into the first external loop;
motifs 4 and 5 lie in the second and third external loops; and motifs 6 and
7 lie at the C-terminus. Two iterations on OWL27.1 were required to reach
convergence, at which point a true set comprising 7 sequences was identified.
 
An update on SPTR37_9f identified a true set of 5 sequences.
Summary Information
5 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
75555555
60000000
50000000
40000000
30000000
20000000
1234567
True Positives
OPRX_CAVPO    OPRX_HUMAN    OPRX_MOUSE    OPRX_PIG      
OPRX_RAT
Sequence Titles
OPRX_CAVPO  Nociceptin receptor (Orphanin FQ receptor) (Kappa-type 3 opioid receptor) (KOR-3) (XOR) - Cavia porcellus (Guinea pig). 
OPRX_HUMAN Nociceptin receptor (Orphanin FQ receptor) (Kappa-type 3 opioid receptor) (KOR-3) - Homo sapiens (Human).
OPRX_MOUSE Nociceptin receptor (Orphanin FQ receptor) (Kappa-type 3 opioid receptor) (KOR-3) (ORGC) (K3 opiate receptor) - Mus musculus (Mouse).
OPRX_PIG Nociceptin receptor (Orphanin FQ receptor) (Kappa-type 3 opioid receptor) (KOR-3) (ORGC) (K3 opiate receptor) - Sus scrofa (Pig).
OPRX_RAT Nociceptin receptor (Orphanin FQ receptor) (Kappa-type 3 opioid receptor) (KOR-3) (ROR-C) (XOR1) - Rattus norvegicus (Rat).
Scan History
OWL27_1    2  50   NSINGLE    
SPTR37_9f 2 6 NSINGLE
Initial Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
APFWEVLYGSHFQ OPRX_MOUSE 7 7 -
APYWEVLYGSHFQ OPRX_RAT 7 7 -
APFWEVIYGSHLQ OPRX_HUMAN 7 7 -

Motif 2 width=19
Element Seqn Id St Int Rpt
PHHLLLNASHSAFLPLGLK OPRX_MOUSE 30 10 -
PHHLLLNASHSAFLPLGLK OPRX_RAT 30 10 -
PPHLLLNASHGAFLPLGLK OPRX_HUMAN 33 13 -

Motif 3 width=9
Element Seqn Id St Int Rpt
QGTDILLGF OPRX_MOUSE 104 55 -
QGTDILLGF OPRX_RAT 104 55 -
QGTDILLGF OPRX_HUMAN 107 55 -

Motif 4 width=19
Element Seqn Id St Int Rpt
IMGSAQVEDEEIECLVEIP OPRX_MOUSE 184 71 -
IMGSAQVEDEEIECLVEIP OPRX_RAT 184 71 -
IMGSAQVEDEEIECLVEIP OPRX_HUMAN 187 71 -

Motif 5 width=18
Element Seqn Id St Int Rpt
QGLGVQPGSETAVAILRF OPRX_MOUSE 283 80 -
QGLGVQPGSETAVAILRF OPRX_RAT 283 80 -
QGLGVQPSSETAVAILRF OPRX_HUMAN 286 80 -

Motif 6 width=13
Element Seqn Id St Int Rpt
CASALHREMQVSD OPRX_MOUSE 332 31 -
CASSLHREMQVSD OPRX_RAT 332 31 -
CASALRRDVQVSD OPRX_HUMAN 335 31 -

Motif 7 width=18
Element Seqn Id St Int Rpt
SIAKDVGLGCKTSETVPR OPRX_MOUSE 348 3 -
SIAKDVGLGCKTSETVPR OPRX_RAT 348 3 -
SIAKDVALACKTSETVPR OPRX_HUMAN 351 3 -
Final Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
APFWEVLYGSHFQ OPRX_MOUSE 7 7 -
APYWEVLYGSHFQ OPRX_RAT 7 7 -
APFWEVLYGSHLQ OPRX_CAVPO 7 7 -
APFWEVIYGSHLQ OPRX_HUMAN 7 7 -
APFWEVLYGSPLQ OPRX_PIG 7 7 -

Motif 2 width=19
Element Seqn Id St Int Rpt
PHHLLLNASHSAFLPLGLK OPRX_MOUSE 30 10 -
PHHLLLNASHSAFLPLGLK OPRX_RAT 30 10 -
PPHLLLNASHSAFLPLGLK OPRX_CAVPO 33 13 -
PPHLLLNASHGAFLPLGLK OPRX_HUMAN 33 13 -
PPHLLLNASHGAFLPLGLK OPRX_PIG 33 13 -

Motif 3 width=9
Element Seqn Id St Int Rpt
QGTDILLGF OPRX_MOUSE 104 55 -
QGTDILLGF OPRX_RAT 104 55 -
QATDILLGF OPRX_CAVPO 107 55 -
QGTDILLGF OPRX_HUMAN 107 55 -
QGTDVLLGF OPRX_PIG 107 55 -

Motif 4 width=19
Element Seqn Id St Int Rpt
IMGSAQVEDEEIECLVEIP OPRX_MOUSE 184 71 -
IMGSAQVEDEEIECLVEIP OPRX_RAT 184 71 -
IMGSAQVEDEEIECLVEIP OPRX_CAVPO 187 71 -
IMGSAQVEDEEIECLVEIP OPRX_HUMAN 187 71 -
IMGSAQVEDEEIECLVEIP OPRX_PIG 187 71 -

Motif 5 width=18
Element Seqn Id St Int Rpt
QGLGVQPGSETAVAILRF OPRX_MOUSE 283 80 -
QGLGVQPGSETAVAILRF OPRX_RAT 283 80 -
QGLGVQPGSETTVAILRF OPRX_CAVPO 286 80 -
QGLGVQPSSETAVAILRF OPRX_HUMAN 286 80 -
QGLGVQPGSETAVAVLRF OPRX_PIG 286 80 -

Motif 6 width=13
Element Seqn Id St Int Rpt
CASALHREMQVSD OPRX_MOUSE 332 31 -
CASSLHREMQVSD OPRX_RAT 332 31 -
CASALHREMQVSD OPRX_CAVPO 335 31 -
CASALRRDVQVSD OPRX_HUMAN 335 31 -
CAPTRRREMQVSD OPRX_PIG 335 31 -

Motif 7 width=18
Element Seqn Id St Int Rpt
SIAKDVGLGCKTSETVPR OPRX_MOUSE 348 3 -
SIAKDVGLGCKTSETVPR OPRX_RAT 348 3 -
SIAKDVALGCKTTETVPR OPRX_CAVPO 351 3 -
SIAKDVALACKTSETVPR OPRX_HUMAN 351 3 -
SIAKDVALACKTSETVPR OPRX_PIG 351 3 -