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PR00544

Identifier
PROGESTRONER  [View Relations]  [View Alignment]  
Accession
PR00544
No. of Motifs
8
Creation Date
09-JUN-1996  (UPDATE 23-JUN-1999)
Title
Progesterone receptor (3C nuclear receptor) signature
Database References
PRINTS; PR00398 STRDHORMONER
INTERPRO; IPR000128
Literature References
1. NUCLEAR RECEPTORS NOMENCLATURE COMMITTEE
A unified nomenclature system for the nuclear receptor superfamily.
CELL 97 161-163 (1999).
 
2. NISHIKAWA, J-I., KITAURA, M., IMAGAWA, M. AND NISHIHARA, T.
Vitamin D receptor contains multiple dimerisation interfaces that
are functionally different.
NUCLEIC ACIDS RES. 23(4) 606-611 (1995).
 
3. DE VOS, P., SCHMITT, J., VERHOEVEN, G. AND STUNNENBERG, G.
Human androgen receptor expressed in HeLa cells activates transcription
in vitro.
NUCLEIC ACIDS RES. 22(7) 1161-1166 (1994).

Documentation
Steroid or nuclear hormone receptors (NRs) constitute an important super-
family of transcription regulators that are involved in widely diverse 
physiological functions, including control of embryonic development, cell
differentiation and homeostasis [1]. Members of the superfamily include the
steroid hormone receptors and receptors for thyroid hormone, retinoids, 
1,25-dihydroxy-vitamin D3 and a variety of other ligands. The proteins 
function as dimeric molecules in nuclei to regulate the transcription of 
target genes in a ligand-responsive manner [2,3]. In addition to C-terminal
ligand-binding domains, these nuclear receptors contain a highly-conserved,
N-terminal zinc-finger that mediates specific binding to target DNA 
sequences, termed ligand-responsive elements. In the absence of ligand,
steroid hormone receptors are thought to be weakly associated with nuclear
components; hormone binding greatly increases receptor affinity.
 
NRs are extremely important in medical research, a large number of them
being implicated in diseases such as cancer, diabetes, hormone resistance
syndromes, etc. [1]. While several NRs act as ligand-inducible transcription
factors, many do not yet have a defined ligand and are accordingly termed 
"orphan" receptors. During the last decade, more than 300 NRs have been
described, many of which are orphans, which cannot easily be named due to 
current nomenclature confusions in the literature. However, a new system 
has recently been introduced in an attempt to rationalise the increasingly 
complex set of names used to describe superfamily members [1].
 
The progesterone receptor consists of 3 functional and structural domains:
an N-terminal (modulatory) domain; a DNA binding domain that mediates
specific binding to target DNA sequences (ligand-responsive elements);
and a hormone binding domain. The N-terminal domain is unique to the 
progesterone receptors and spans approximately the first 500 residues;
the highly-conserved DNA-binding domain is smaller (around 65 residues)
and occupies the central portion of the protein; and the hormone binding
domain lies at the receptor C-terminus.
 
PROGESTRONER is an 8-element fingerprint that provides a signature for the
progesterone receptors. The fingerprint was derived from an initial
alignment of 4 sequences: the motifs span the N-terminal modulatory domain
preceding the highly-conserved zinc-binding region shared by the steroid
hormone receptor family. Two iterations on OWL28.0 were required to reach
convergence, at which point a true set comprising 7 sequences was
identified. Three partial matches were also found (RNU06637, CHKPGRE1E11
and CHKPGRRA1), all of which are androgen receptor fragments.
 
An update on SPTR37_9f identified a true set of 5 sequences, and 1
partial match.
Summary Information
   5 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
1 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
855555555
700000000
600000000
500011111
400000000
300000000
200000000
12345678
True Positives
PRGR_CHICK    PRGR_HUMAN    PRGR_MOUSE    PRGR_RABIT    
Q63449
True Positive Partials
Codes involving 5 elements
Q90946
Sequence Titles
PRGR_CHICK  PROGESTERONE RECEPTOR (PR) - GALLUS GALLUS (CHICKEN). 
PRGR_HUMAN PROGESTERONE RECEPTOR (PR) - HOMO SAPIENS (HUMAN).
PRGR_MOUSE PROGESTERONE RECEPTOR (PR) - MUS MUSCULUS (MOUSE).
PRGR_RABIT PROGESTERONE RECEPTOR (PR) - ORYCTOLAGUS CUNICULUS (RABBIT).
Q63449 PROGESTERONE RECEPTOR - RATTUS NORVEGICUS (RAT).

Q90946 PROGESTERONE RECEPTOR - GALLUS GALLUS (CHICKEN).
Scan History
OWL28_0    2  25   NSINGLE    
SPTR37_9f 1 50 NSINGLE
Initial Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
VSAIPISLDGLLFPRPC PRGR_HUMAN 48 48 -
VSAIPISLDGLLFPRPC PRGR_RABIT 49 49 -
VSPIPISLDGLLFPRSC PRGR_MOUSE 49 49 -
AEGIDVALDGLLYPRSS PRGR_CHICK 31 31 -

Motif 2 width=18
Element Seqn Id St Int Rpt
PEKDSGLLDSVLDTLLAP PRGR_HUMAN 104 39 -
PEKDSGLLDSVLDTLLAP PRGR_RABIT 105 39 -
PEKDSRLLDSVLDSLLTP PRGR_MOUSE 105 39 -
GSLSKDCLDSVLDTFLAP PRGR_CHICK 89 41 -

Motif 3 width=15
Element Seqn Id St Int Rpt
TSSWCLFGPELPEDP PRGR_HUMAN 137 15 -
ISPWCLFGPDLPEDP PRGR_RABIT 138 15 -
ITSWCLFGPELPEDP PRGR_MOUSE 138 15 -
AAPWSLFGPEVPEVP PRGR_CHICK 110 3 -

Motif 4 width=23
Element Seqn Id St Int Rpt
DFIHVPILPLNHALLAARTRQLL PRGR_HUMAN 302 150 -
DFIHVPILPLNHAFLATRTRQLL PRGR_RABIT 301 148 -
DFIHVPILPLNHALLAARTRQLL PRGR_MOUSE 302 149 -
DYLHVPILPLNSAFLASRTRQLL PRGR_CHICK 221 96 -

Motif 5 width=17
Element Seqn Id St Int Rpt
SSTPVAVGDFPDCAYPP PRGR_HUMAN 349 24 -
SSTPVAGGDFPDCTYPP PRGR_RABIT 348 24 -
PSTPVPRGDFPDCTYPL PRGR_MOUSE 347 22 -
SSPSVPAADLAEYGYPP PRGR_CHICK 258 14 -

Motif 6 width=17
Element Seqn Id St Int Rpt
GAAPALYPALGLNGLPQ PRGR_HUMAN 506 140 -
GAAPALYPTLGLNGLPQ PRGR_RABIT 507 142 -
AAAPAIYQPLGLNGLPQ PRGR_MOUSE 496 132 -
AAPPGLYSPLGLNGHHQ PRGR_CHICK 359 84 -

Motif 7 width=20
Element Seqn Id St Int Rpt
LGYQAAVLKEGLPQVYPPYL PRGR_HUMAN 523 0 -
LGYQAAVLKEGLPQVYTPYL PRGR_RABIT 524 0 -
LGYQAAVLKDSLPQVYPPYL PRGR_MOUSE 513 0 -
LGFPAAVLKEGLPQLCPPYL PRGR_CHICK 377 1 -

Motif 8 width=20
Element Seqn Id St Int Rpt
YLRPDSEASQSPQYSFESLP PRGR_HUMAN 544 1 -
YLRPDSEASQSPQYSFESLP PRGR_RABIT 545 1 -
YLRPDSEASQSPQYGFDSLP PRGR_MOUSE 534 1 -
YVRPDTETSQSSQYSFESLP PRGR_CHICK 398 1 -
Final Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
VSAIPISLDGLLFPRPC PRGR_HUMAN 48 48 -
VSAIPISLDGLLFPRPC PRGR_RABIT 49 49 -
VSPIPISLDRLLFSRSC Q63449 49 49 -
VSPIPISLDGLLFPRSC PRGR_MOUSE 49 49 -
AEGIDVALDGLLYPRSS PRGR_CHICK 31 31 -

Motif 2 width=18
Element Seqn Id St Int Rpt
PEKDSGLLDSVLDTLLAP PRGR_HUMAN 104 39 -
PEKDSGLLDSVLDTLLAP PRGR_RABIT 105 39 -
PEKDSRLLDSVLDTLLAP Q63449 104 38 -
PEKDSRLLDSVLDSLLTP PRGR_MOUSE 105 39 -
GSLSKDCLDSVLDTFLAP PRGR_CHICK 89 41 -

Motif 3 width=15
Element Seqn Id St Int Rpt
TSSWCLFGPELPEDP PRGR_HUMAN 137 15 -
ISPWCLFGPDLPEDP PRGR_RABIT 138 15 -
ITSWCLFGPELPEDP Q63449 137 15 -
ITSWCLFGPELPEDP PRGR_MOUSE 138 15 -
AAPWSLFGPEVPEVP PRGR_CHICK 110 3 -

Motif 4 width=23
Element Seqn Id St Int Rpt
DFIHVPILPLNHALLAARTRQLL PRGR_HUMAN 302 150 -
DFIHVPILPLNHAFLATRTRQLL PRGR_RABIT 301 148 -
DFIHVPILPLNHALLAARTRQLL Q63449 301 149 -
DFIHVPILPLNHALLAARTRQLL PRGR_MOUSE 302 149 -
DYLHVPILPLNSAFLASRTRQLL PRGR_CHICK 221 96 -

Motif 5 width=17
Element Seqn Id St Int Rpt
SSTPVAVGDFPDCAYPP PRGR_HUMAN 349 24 -
SSTPVAGGDFPDCTYPP PRGR_RABIT 348 24 -
PSPPVPCGDFPDCTYPP Q63449 348 24 -
PSTPVPRGDFPDCTYPL PRGR_MOUSE 347 22 -
SSPSVPAADLAEYGYPP PRGR_CHICK 258 14 -

Motif 6 width=17
Element Seqn Id St Int Rpt
GAAPALYPALGLNGLPQ PRGR_HUMAN 506 140 -
GAAPALYPTLGLNGLPQ PRGR_RABIT 507 142 -
SAAPAIYPPLGLNGLPQ Q63449 496 131 -
AAAPAIYQPLGLNGLPQ PRGR_MOUSE 496 132 -
AAPPGLYSPLGLNGHHQ PRGR_CHICK 359 84 -

Motif 7 width=20
Element Seqn Id St Int Rpt
LGYQAAVLKEGLPQVYPPYL PRGR_HUMAN 523 0 -
LGYQAAVLKEGLPQVYTPYL PRGR_RABIT 524 0 -
LGYQAAVLKDSLPQVYPPYL Q63449 513 0 -
LGYQAAVLKDSLPQVYPPYL PRGR_MOUSE 513 0 -
LGFPAAVLKEGLPQLCPPYL PRGR_CHICK 377 1 -

Motif 8 width=20
Element Seqn Id St Int Rpt
YLRPDSEASQSPQYSFESLP PRGR_HUMAN 544 1 -
YLRPDSEASQSPQYSFESLP PRGR_RABIT 545 1 -
YLRPDSEASQSPQYGFDSLP Q63449 534 1 -
YLRPDSEASQSPQYGFDSLP PRGR_MOUSE 534 1 -
YVRPDTETSQSSQYSFESLP PRGR_CHICK 398 1 -