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PR00543

Identifier
OESTROGENR  [View Relations]  [View Alignment]  
Accession
PR00543
No. of Motifs
8
Creation Date
08-JUN-1996  (UPDATE 07-JUN-1999)
Title
Oestrogen receptor (3A nuclear receptor) signature
Database References
PRINTS; PR00398 STRDHORMONER
INTERPRO; IPR001292
Literature References
1. NUCLEAR RECEPTORS NOMENCLATURE COMMITTEE
A unified nomenclature system for the nuclear receptor superfamily.
CELL 97 161-163 (1999).
 
2. NISHIKAWA, J-I., KITAURA, M., IMAGAWA, M. AND NISHIHARA, T.
Vitamin D receptor contains multiple dimerisation interfaces that
are functionally different.
NUCLEIC ACIDS RES. 23(4) 606-611 (1995).
 
3. DE VOS, P., SCHMITT, J., VERHOEVEN, G. AND STUNNENBERG, G.
Human androgen receptor expressed in HeLa cells activates transcription
in vitro.
NUCLEIC ACIDS RES. 22(7) 1161-1166 (1994).
 
4. DANIELIAN, P.S., WHITE, R., LEES, J.A. AND PARKER, M.G.
Identification of a conserved region required for hormone dependent
transcriptional activation by steroid hormone receptors.
EMBO J. 11 1025-1033 (1992).

Documentation
Steroid or nuclear hormone receptors (NRs) constitute an important super-
family of transcription regulators that are involved in widely diverse 
physiological functions, including control of embryonic development, cell
differentiation and homeostasis [1]. Members of the superfamily include the
steroid hormone receptors and receptors for thyroid hormone, retinoids, 
1,25-dihydroxy-vitamin D3 and a variety of other ligands. The proteins 
function as dimeric molecules in nuclei to regulate the transcription of 
target genes in a ligand-responsive manner [2,3]. In addition to C-terminal
ligand-binding domains, these nuclear receptors contain a highly-conserved,
N-terminal zinc-finger that mediates specific binding to target DNA 
sequences, termed ligand-responsive elements. In the absence of ligand,
steroid hormone receptors are thought to be weakly associated with nuclear
components; hormone binding greatly increases receptor affinity.
 
NRs are extremely important in medical research, a large number of them
being implicated in diseases such as cancer, diabetes, hormone resistance
syndromes, etc. [1]. While several NRs act as ligand-inducible transcription
factors, many do not yet have a defined ligand and are accordingly termed 
"orphan" receptors. During the last decade, more than 300 NRs have been
described, many of which are orphans, which cannot easily be named due to 
current nomenclature confusions in the literature. However, a new system 
has recently been introduced in an attempt to rationalise the increasingly 
complex set of names used to describe superfamily members [1].
 
The oestrogen receptor consists of 3 functional and structural domains:
an N-terminal (modulatory) domain; a DNA binding domain that mediates
specific binding to target DNA sequences, termed ligand-responsive
elements; and a hormone binding domain. The N-terminal domain is unique to
the oestrogen receptors and spans approximately the first 180 residues; the
highly-conserved DNA-binding domain is smaller (around 65 residues) and
occupies the central portion of the protein; and the hormone binding
domain lies at the receptor C-terminus. 
 
The oestrogen receptor [4] stimulates transcription via 2 distinct
transcriptional activation domains: TAF-1 in the N-terminal domain, and
TAF-2 in the hormone-binding domain. TAF-2 activity requires a region in
the C-terminus of the hormone-binding domain (between residues 538-552 in
the mouse oestrogen receptor) that is conserved in many nuclear hormone
receptors. It is therefore suggested that this region may be essential
for ligand-dependent transcriptional activation by other members of the
nuclear receptor family [3].
 
OESTROGENR is an 8-element fingerprint that provides a signature for the
oestrogen receptors. The fingerprint was derived from an initial alignment
of 4 sequences: the motifs span the N-terminal modulatory domain preceding
the highly-conserved zinc-binding region shared by the steroid hormone
receptor family. Two iterations on OWL28.0 were required to reach
convergence, at which point a true set comprising 8 sequences was
identified. A single partial match was also found (ORZMER), an oestrogen
receptor from the killifish that matches motifs 5 and 6.
 
An update on SPTR57.15_40.15f identified a true set of 46 sequences,
together with numerous partial matches, all of which are family members
that fail to match one or more motifs.
Summary Information
10 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
81010101010101010
700000000
600000000
500000000
400000000
300000000
200000000
12345678
True Positives
ESTR_CHICK    ESTR_HUMAN    ESTR_MOUSE    ESTR_PIG      
ESTR_RAT P81559 Q13511 Q14267
Q14268 Q91250
Sequence Titles
ESTR_CHICK  ESTROGEN RECEPTOR (ER) (ESTRADIOL RECEPTOR) - GALLUS GALLUS (CHICKEN). 
ESTR_HUMAN ESTROGEN RECEPTOR (ER) (ESTRADIOL RECEPTOR) - HOMO SAPIENS (HUMAN).
ESTR_MOUSE ESTROGEN RECEPTOR (ER) (ESTRADIOL RECEPTOR) - MUS MUSCULUS (MOUSE).
ESTR_PIG ESTROGEN RECEPTOR (ER) (ESTRADIOL RECEPTOR) - SUS SCROFA (PIG).
ESTR_RAT ESTROGEN RECEPTOR (ER) (ESTRADIOL RECEPTOR) - RATTUS NORVEGICUS (RAT).
P81559 ESTROGEN RECEPTOR (ER) (ESTRADIOL RECEPTOR) - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
Q13511 80 KDA ESTROGEN RECEPTOR - HOMO SAPIENS (HUMAN).
Q14267 ESTROGEN RECEPTOR-RELATED PROTEIN - HOMO SAPIENS (HUMAN).
Q14268 ESTROGEN RECEPTOR-RELATED PROTEIN - HOMO SAPIENS (HUMAN).
Q91250 ESTROGEN RECEPTOR (ER) (ESTRADIOL RECEPTOR) - POEPHILA GUTTATA (ZEBRA FINCH) (TAENIOPYGIA GUTTATA).
Scan History
OWL28_0    2  100  NSINGLE    
SPTR37_9f 2 11 NSINGLE
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
HTKASGMALLHQIQGNELE ESTR_HUMAN 6 6 -
HTKASGMALLHQIQGNELE ESTR_MOUSE 6 6 -
HTKASGMALLHQIQGNELE ESTR_RAT 6 6 -
HTKASGVTLLHQIQGTELE ESTR_CHICK 6 6 -

Motif 2 width=18
Element Seqn Id St Int Rpt
LNRPQLKIPLERPLGEVY ESTR_HUMAN 26 1 -
LNRPQLKMPMERALGEVY ESTR_MOUSE 26 1 -
LNRPQLKMPMERALGEVY ESTR_RAT 26 1 -
LSRPQLKIPLERSLSDMY ESTR_CHICK 26 1 -

Motif 3 width=18
Element Seqn Id St Int Rpt
DSSKPAVYNYPEGAAYEF ESTR_HUMAN 45 1 -
DNSKPTVFNYPEGAAYEF ESTR_MOUSE 45 1 -
DNSKPAVFNYPEGAAYEF ESTR_RAT 45 1 -
ESNKTGVFNYPEGATYDF ESTR_CHICK 45 1 -

Motif 4 width=16
Element Seqn Id St Int Rpt
AQVYGQTGLPYGPGSE ESTR_HUMAN 70 7 -
APVYGQSGIAYGPGSE ESTR_MOUSE 74 11 -
APVYGQSSITYGPGSE ESTR_RAT 75 12 -
APVYGSTTLSYAPTSE ESTR_CHICK 66 3 -

Motif 5 width=15
Element Seqn Id St Int Rpt
FPPLNSVSPSPLMLL ESTR_HUMAN 97 11 -
FPQLNSVSPSPLMLL ESTR_MOUSE 101 11 -
FPQLNSVSPSPLMLL ESTR_RAT 102 11 -
FHSLNNVPPSPVVFL ESTR_CHICK 91 9 -

Motif 6 width=17
Element Seqn Id St Int Rpt
PQLSPFLQPHGQQVPYY ESTR_HUMAN 115 3 -
PQLSPFLHPHGQQVPYY ESTR_MOUSE 119 3 -
PHVSPFLHPHGHQVPYY ESTR_RAT 120 3 -
PQLSPFIHHHSQQVPYY ESTR_CHICK 109 3 -

Motif 7 width=20
Element Seqn Id St Int Rpt
LENEPSGYTVREAGPPAFYR ESTR_HUMAN 132 0 -
LENEPSAYAVRDTGPPAFYR ESTR_MOUSE 136 0 -
LENEPSAYAVRDTGPPAFYR ESTR_RAT 137 0 -
LENEQGSFGMREAAPPAFYR ESTR_CHICK 126 0 -

Motif 8 width=20
Element Seqn Id St Int Rpt
NSDNRRQGGRERLASTNDKG ESTR_HUMAN 153 1 -
NSDNRRQNGRERLSSSNEKG ESTR_MOUSE 157 1 -
NSDNRRQNGRERLSSSSEKG ESTR_RAT 158 1 -
SSDNRRHSIRERMSSTNEKG ESTR_CHICK 147 1 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
HTKASGMALLHQIQGNELE ESTR_HUMAN 6 6 -
HTKASGMALLHQIQGNELE Q14268 6 6 -
HTKASGMALLHQIQGNELE Q14267 6 6 -
HTKASGMALLHQIQGNELE Q13511 6 6 -
HTKASGMALLHQIQANELE ESTR_PIG 6 6 -
HTKASGMALLHQIQGNELE ESTR_MOUSE 6 6 -
HTKASGMALLHQIQGNELE ESTR_RAT 6 6 -
HTKASGVTLLHQIQGTELE ESTR_CHICK 6 6 -
HTKTSGVTLLHQIQGTELE Q91250 4 4 -
PNKTTGVTFLHQIQSSELE P81559 6 6 -

Motif 2 width=18
Element Seqn Id St Int Rpt
LNRPQLKIPLERPLGEVY ESTR_HUMAN 26 1 -
LNRPQLKIPLERPLGEVY Q14268 26 1 -
LNRPQLKIPLERPLGEVY Q14267 26 1 -
LNRPQLKIPLERPLGEVY Q13511 26 1 -
LNRPQLKIPLERPLGEVY ESTR_PIG 26 1 -
LNRPQLKMPMERALGEVY ESTR_MOUSE 26 1 -
LNRPQLKMPMERALGEVY ESTR_RAT 26 1 -
LSRPQLKIPLERSLSDMY ESTR_CHICK 26 1 -
LSRPQLKIPLERSLSDMY Q91250 24 1 -
LTRPPLKISLERPLGEMY P81559 26 1 -

Motif 3 width=18
Element Seqn Id St Int Rpt
DSSKPAVYNYPEGAAYEF ESTR_HUMAN 45 1 -
DSSKPAVYNYPEGAAYEF Q14268 45 1 -
DSSKPAVYNYPEGAAYEF Q14267 45 1 -
DSSKPAVYNYPEGAAYEF Q13511 45 1 -
DSSKPAVYNYPEGAAYDF ESTR_PIG 45 1 -
DNSKPTVFNYPEGAAYEF ESTR_MOUSE 45 1 -
DNSKPAVFNYPEGAAYEF ESTR_RAT 45 1 -
ESNKTGVFNYPEGATYDF ESTR_CHICK 45 1 -
ETNKTGVFNYPEGATYDF Q91250 43 1 -
ENNRTGIFNYPEGTTYDF P81559 45 1 -

Motif 4 width=16
Element Seqn Id St Int Rpt
AQVYGQTGLPYGPGSE ESTR_HUMAN 70 7 -
AQVYGQTGLPYGPGSE Q14268 70 7 -
AQVYGQTGLPYGPGSE Q14267 70 7 -
AQVYGQTGLPYGPGSE Q13511 70 7 -
APVYGQSGLAYGPGSE ESTR_PIG 70 7 -
APVYGQSGIAYGPGSE ESTR_MOUSE 74 11 -
APVYGQSSITYGPGSE ESTR_RAT 75 12 -
APVYGSTTLSYAPTSE ESTR_CHICK 66 3 -
APVYSSTTLSYAPTSE Q91250 64 3 -
APVYSSASLSYAASSE P81559 67 4 -

Motif 5 width=15
Element Seqn Id St Int Rpt
FPPLNSVSPSPLMLL ESTR_HUMAN 97 11 -
FPPLNSVSPSPLMLL Q14268 97 11 -
FPPLNSVSPSPLMLL Q14267 97 11 -
FPPLNSVSPSPLMLL Q13511 97 11 -
FQPLNSVSPSPLVLL ESTR_PIG 97 11 -
FPQLNSVSPSPLMLL ESTR_MOUSE 101 11 -
FPQLNSVSPSPLMLL ESTR_RAT 102 11 -
FHSLNNVPPSPVVFL ESTR_CHICK 91 9 -
FHSLNSVPPSPVVFL Q91250 89 9 -
LHTLNNVPPSPVVFL P81559 92 9 -

Motif 6 width=17
Element Seqn Id St Int Rpt
PQLSPFLQPHGQQVPYY ESTR_HUMAN 115 3 -
PQLSPFLQPHGQQVPYY Q14268 115 3 -
PQLSPFLQPHGQQVPYY Q14267 115 3 -
PQLSPFLQPHGQQVPYY Q13511 115 3 -
PQLSPFLHPHGQQVPYY ESTR_PIG 115 3 -
PQLSPFLHPHGQQVPYY ESTR_MOUSE 119 3 -
PHVSPFLHPHGHQVPYY ESTR_RAT 120 3 -
PQLSPFIHHHSQQVPYY ESTR_CHICK 109 3 -
PHWSPFIHHHSQQVPYY Q91250 107 3 -
PQLSPFIHHHGQQVPYY P81559 110 3 -

Motif 7 width=20
Element Seqn Id St Int Rpt
LENEPSGYTVREAGPPAFYR ESTR_HUMAN 132 0 -
LENEPSGYTVREAGPPAFYR Q14268 132 0 -
LENEPSGYTVREAGPPAFYR Q14267 132 0 -
LENEPSGYTVREAGPPAFYR Q13511 132 0 -
LENEPSGYAVREAGPPAFYR ESTR_PIG 132 0 -
LENEPSAYAVRDTGPPAFYR ESTR_MOUSE 136 0 -
LENEPSAYAVRDTGPPAFYR ESTR_RAT 137 0 -
LENEQGSFGMREAAPPAFYR ESTR_CHICK 126 0 -
LENDQGSFGMREAAPPAFYR Q91250 124 0 -
LESEQGTFAVREAAPPTFYR P81559 127 0 -

Motif 8 width=20
Element Seqn Id St Int Rpt
NSDNRRQGGRERLASTNDKG ESTR_HUMAN 153 1 -
NSDNRRQGGRERLASTNDKG Q14268 153 1 -
NSDNRRQGGRERLASTNDKG Q14267 153 1 -
NSDNRRQGGRERLASTNDKG Q13511 153 1 -
NSDNRRQGGRERLASTSDKG ESTR_PIG 153 1 -
NSDNRRQNGRERLSSSNEKG ESTR_MOUSE 157 1 -
NSDNRRQNGRERLSSSSEKG ESTR_RAT 158 1 -
SSDNRRHSIRERMSSTNEKG ESTR_CHICK 147 1 -
NSDNRRHSIRERMSSANEKG Q91250 145 1 -
SSDNRRQSGRERMSSANDKG P81559 148 1 -