SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR00531

Identifier
HISTAMINEH2R  [View Relations]  [View Alignment]  
Accession
PR00531
No. of Motifs
7
Creation Date
21-MAY-1996  (UPDATE 07-JUN-1999)
Title
Histamine H2 receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN

INTERPRO; IPR000503
GCRDB; GCR_0176; GCR_0014; GCR_0308
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Histamine.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.152-158.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Histamine is distributed within mast cells in all peripheral tissues and
is a well-characterised mediator of inflammation and allergy [6]. It also
regulates release of gastric acid from parietal cells in the gastric
mucosa. H2 receptors are found in high levels in the stomach and heart,
and have limited distribution in smooth muscle and cells of the immune
system. H2 antagonists are used clinically in the treatment of peptic
ulceration [6].
 
HISTAMINEH2R is a 7-element fingerprint that provides a signature for the
histamine H2 receptors. The fingerprint was derived from an initial
alignment of 4 sequences: the motifs were drawn from conserved sections
within either loop or N- and C-terminal regions, focusing on those areas
of the alignment that characterise the H2 receptors but distinguish them
from the H1 receptors - motif 1 lies in the N-terminal region immediately
preceding TM domain 1; motif 2 spans part of the second external loop
and the N-terminus of TM domain 5; motif 3 lies in the third cytoplasmic
loop; motif 4 spans the third external loop; and motifs 5-7 lie in the
C-terminal region following TM domain 7. A single iteration on OWL27.1 was
required to reach convergence, no further sequences being identified beyond
the starting set. 
 
An update on SPTR37_9f identified a true set of 5 sequences.
Summary Information
5 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
75555555
60000000
50000000
40000000
30000000
20000000
1234567
True Positives
HH2R_CANFA    HH2R_CAVPO    HH2R_HUMAN    HH2R_MOUSE    
HH2R_RAT
Sequence Titles
HH2R_CANFA  HISTAMINE H2 RECEPTOR (GASTRIC RECEPTOR I) - CANIS FAMILIARIS (DOG). 
HH2R_CAVPO HISTAMINE H2 RECEPTOR (GASTRIC RECEPTOR I) - CAVIA PORCELLUS (GUINEA PIG).
HH2R_HUMAN HISTAMINE H2 RECEPTOR (GASTRIC RECEPTOR I) - HOMO SAPIENS (HUMAN).
HH2R_MOUSE HISTAMINE H2 RECEPTOR (GASTRIC RECEPTOR I) - MUS MUSCULUS (MOUSE).
HH2R_RAT HISTAMINE H2 RECEPTOR (GASTRIC RECEPTOR I) - RATTUS NORVEGICUS (RAT).
Scan History
OWL27_1    1  50   NSINGLE    
SPTR37_9f 2 6 NSINGLE
Initial Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
NGTASSFCLDSTACKIT HH2R_HUMAN 4 4 -
NGTGSSFCLDSPPCRIT HH2R_CANFA 4 4 -
NGTVPSFCMDFTVYKVT JC4120 4 4 -
NGTVHSCCLDSMALKVT HH2R_RAT 4 4 -

Motif 2 width=16
Element Seqn Id St Int Rpt
KVQVNLVYGLVDGLVT HH2R_CANFA 175 154 -
KVQVNEVYGLVDGLVT HH2R_HUMAN 175 154 -
KVQVNEVYGLVDGLVT JC4120 175 154 -
KVQVNEVYGLVDGLVT HH2R_RAT 174 153 -

Motif 3 width=17
Element Seqn Id St Int Rpt
DQAKRINHISSWKAATI HH2R_HUMAN 211 20 -
EQARRINHIGSWKAATI JC4120 211 20 -
EQAKRINHISSWKAATI HH2R_RAT 210 20 -
DQAKRIHHMGSWKAATI HH2R_CANFA 211 20 -

Motif 4 width=16
Element Seqn Id St Int Rpt
VYRGLRGDDAINEAVE HH2R_RAT 254 27 -
VYRGLKGDDAINEAFE HH2R_CANFA 255 27 -
VYRGLKGDDAVNEVFE JC4120 255 27 -
VYRGLRGDDAINEVLE HH2R_HUMAN 255 27 -

Motif 5 width=19
Element Seqn Id St Int Rpt
RDFRTAYHQLFCCRLASHN JC4120 293 22 -
RDFRTAYQQLFHCKFASHN HH2R_RAT 292 22 -
RDFRTGYQQLFCCRLANRN HH2R_HUMAN 293 22 -
RDFRTAYQQLFRCRPASHN HH2R_CANFA 293 22 -

Motif 6 width=18
Element Seqn Id St Int Rpt
TSLRSNSSQLARNQSREP HH2R_CANFA 315 3 -
TSLRLNNSQLNRSQCQEP JC4120 315 3 -
TSLRLNNSLLPRSQSREG HH2R_RAT 314 3 -
TSLRSNASQLSRTQSREP HH2R_HUMAN 315 3 -

Motif 7 width=19
Element Seqn Id St Int Rpt
QEEKPLKLQVWSGTELTHP HH2R_RAT 334 2 -
QEEKPLKLQVWSGTEVTAP HH2R_CANFA 335 2 -
QEEKPLKLQVWSGTEVTAP HH2R_HUMAN 335 2 -
QEDKPLNLQVWSGTEVTAP JC4120 335 2 -
Final Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
NGTVHSCCLDSMALKVT HH2R_RAT 4 4 -
NGTVHSCCLDSIALKVT HH2R_MOUSE 4 4 -
NGTASSFCLDSTACKIT HH2R_HUMAN 4 4 -
NGTGSSFCLDSPPCRIT HH2R_CANFA 4 4 -
NGTVPSFCMDFTVYKVT HH2R_CAVPO 4 4 -

Motif 2 width=16
Element Seqn Id St Int Rpt
KVQVNEVYGLVDGLVT HH2R_RAT 174 153 -
KVQVNEVYGLVDGMVT HH2R_MOUSE 174 153 -
KVQVNEVYGLVDGLVT HH2R_HUMAN 175 154 -
KVQVNLVYGLVDGLVT HH2R_CANFA 175 154 -
KVQVNEVYGLVDGLVT HH2R_CAVPO 175 154 -

Motif 3 width=17
Element Seqn Id St Int Rpt
EQAKRINHISSWKAATI HH2R_RAT 210 20 -
EQAKRINHISSWKAATI HH2R_MOUSE 210 20 -
DQAKRINHISSWKAATI HH2R_HUMAN 211 20 -
DQAKRIHHMGSWKAATI HH2R_CANFA 211 20 -
EQARRINHIGSWKAATI HH2R_CAVPO 211 20 -

Motif 4 width=16
Element Seqn Id St Int Rpt
VYRGLRGDDAINEAVE HH2R_RAT 254 27 -
VYRGLRGDDPVNEVVE HH2R_MOUSE 254 27 -
VYRGLRGDDAINEVLE HH2R_HUMAN 255 27 -
VYRGLKGDDAINEAFE HH2R_CANFA 255 27 -
VYRGLKGDDAVNEVFE HH2R_CAVPO 255 27 -

Motif 5 width=19
Element Seqn Id St Int Rpt
RDFRTAYQQLFHCKFASHN HH2R_RAT 292 22 -
RDFRMAYQQLFHCKLASHN HH2R_MOUSE 292 22 -
RDFRTGYQQLFCCRLANRN HH2R_HUMAN 293 22 -
RDFRTAYQQLFRCRPASHN HH2R_CANFA 293 22 -
RDFRTAYHQLFCCRLASHN HH2R_CAVPO 293 22 -

Motif 6 width=18
Element Seqn Id St Int Rpt
TSLRLNNSLLPRSQSREG HH2R_RAT 314 3 -
TSLRLNNSLLSRSQSREG HH2R_MOUSE 314 3 -
TSLRSNASQLSRTQSREP HH2R_HUMAN 315 3 -
TSLRSNSSQLARNQSREP HH2R_CANFA 315 3 -
TSLRLNNSQLNRSQCQEP HH2R_CAVPO 315 3 -

Motif 7 width=19
Element Seqn Id St Int Rpt
QEEKPLKLQVWSGTELTHP HH2R_RAT 334 2 -
QEEKPLKLQVWSGTELTHP HH2R_MOUSE 334 2 -
QEEKPLKLQVWSGTEVTAP HH2R_HUMAN 335 2 -
QEEKPLKLQVWSGTEVTAP HH2R_CANFA 335 2 -
QEDKPLNLQVWSGTEVTAP HH2R_CAVPO 335 2 -