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PR00530

Identifier
HISTAMINEH1R  [View Relations]  [View Alignment]  
Accession
PR00530
No. of Motifs
9
Creation Date
21-MAY-1996  (UPDATE 07-JUN-1999)
Title
Histamine H1 receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN

INTERPRO; IPR000921
GCRDB; GCR_0879; GCR_0722; GCR_0541; GCR_0278
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Histamine.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.152-158.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Histamine is distributed within mast cells in all peripheral tissues and
is a well-characterised mediator of inflammation and allergy [6]. It also
regulates release of gastric acid from parietal cells in the gastric
mucosa. H1 receptors mediate increase in vascular permeability induced
by histamine at sites of inflammation. H1 antagonists are used clinically
in the treatment of allergic and anaphylactic reactions, various
inflammatory conditions, and motion sickness. The receptor is distributed
widely in the periphery, notably smooth muscle, where it stimulates
contraction, and also in the adrenal medulla, vascular endothelium and
heart. It is found throughout the CNS, including the cerebral cortex, 
spinal cord and cerebellum.
 
HISTAMINEH1R is a 9-element fingerprint that provides a signature for the
histamine H1 receptors. The fingerprint was derived from an initial
alignment of 4 sequences: the motifs were drawn from conserved sections
within either loop or C-terminal regions, focusing on those areas of the
alignment that characterise the H1 receptors but distinguish them from the
H2 receptors - motif 1 spans the C-terminus of TM domain 4 and part of the
second external loop; motif 2 spans part of the second external loop and
the N-terminus of TM domain 5; motifs 3-7 lie in the third cytoplasmic loop
motif 8 spans the C-terminus of TM domain 6 and part of the third external
loop; and motif 9 lies in the C-terminal region following TM domain 7. A
single iteration on OWL27.1 was required to reach convergence, no further
sequences being identified beyond the starting set. 
 
An update on SPTR37_9f identified a true set of 5 sequences.
Summary Information
5 codes involving  9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
9555555555
8000000000
7000000000
6000000000
5000000000
4000000000
3000000000
2000000000
123456789
True Positives
HH1R_BOVIN    HH1R_CAVPO    HH1R_HUMAN    HH1R_MOUSE    
HH1R_RAT
Sequence Titles
HH1R_BOVIN  HISTAMINE H1 RECEPTOR - BOS TAURUS (BOVINE).  
HH1R_CAVPO HISTAMINE H1 RECEPTOR - CAVIA PORCELLUS (GUINEA PIG).
HH1R_HUMAN HISTAMINE H1 RECEPTOR - HOMO SAPIENS (HUMAN).
HH1R_MOUSE HISTAMINE H1 RECEPTOR - MUS MUSCULUS (MOUSE).
HH1R_RAT HISTAMINE H1 RECEPTOR - RATTUS NORVEGICUS (RAT).
Scan History
OWL27_1    1  50   NSINGLE    
SPTR37_9f 2 6 NSINGLE
Initial Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
SFLWVIPILGWHHF HH1R_RAT 155 155 -
SFLWVIPILGWNHF HH1R_HUMAN 155 155 -
SFLWIIPILGWRHF HH1R_BOVIN 156 156 -
SFLWVIPILGWHHF HH1R_CAVPO 164 164 -

Motif 2 width=19
Element Seqn Id St Int Rpt
CETDFYNVTWFKIMTAIIN HH1R_RAT 180 11 -
CETDFYDVTWFKVMTAIIN HH1R_HUMAN 180 11 -
CETDFYNVTWFKVMTAIIN HH1R_BOVIN 181 11 -
CETDFYDVTWFKVMTAIIN HH1R_CAVPO 189 11 -

Motif 3 width=19
Element Seqn Id St Int Rpt
HCQHRQLTNGSLPSFSELK HH1R_RAT 220 21 -
HCQHRELINRSLPSFSEIK HH1R_HUMAN 220 21 -
HCQHRELINGSFPSFSDMK HH1R_BOVIN 221 21 -
HCQHRQLINSSLPSFSEMK HH1R_CAVPO 229 21 -

Motif 4 width=18
Element Seqn Id St Int Rpt
KKPGRESPWGVLKRPSRD HH1R_RAT 249 10 -
KKPGKESPWEVLKRKPKD HH1R_HUMAN 249 10 -
KKPGKESPWEVLKRKPKD HH1R_BOVIN 250 10 -
RRMGKESPWEDPKRCSKD HH1R_CAVPO 258 10 -

Motif 5 width=14
Element Seqn Id St Int Rpt
EDQTLVDQQSFSRT HH1R_RAT 346 79 -
EDQMLGDSQSFSRT HH1R_HUMAN 348 81 -
EDQILGDSQSFSRT HH1R_BOVIN 352 84 -
EEWTVVDGQSFPIT HH1R_CAVPO 349 73 -

Motif 6 width=15
Element Seqn Id St Int Rpt
RSGSNSGLDYIKITW HH1R_RAT 376 16 -
RSGSNTGLDYIKFTW HH1R_HUMAN 377 15 -
RSESSTGLEYIKFTW HH1R_BOVIN 381 15 -
RSGSNSGLDYIKFTW HH1R_CAVPO 378 15 -

Motif 7 width=18
Element Seqn Id St Int Rpt
WKRLRSHSRQYVSGLHLN HH1R_RAT 390 -1 -
WKRLRSHSRQYVSGLHMN HH1R_HUMAN 391 -1 -
WKRLRSHSRQYVSGLHMN HH1R_BOVIN 395 -1 -
WRRLRSHSRQYTSGLHLN HH1R_CAVPO 392 -1 -

Motif 8 width=12
Element Seqn Id St Int Rpt
FFMVIAFCKSCC HH1R_RAT 433 25 -
FFMVIAFCKNCC HH1R_HUMAN 434 25 -
FFMVIAFCESCC HH1R_BOVIN 438 25 -
FFMVIAFCKSCS HH1R_CAVPO 435 25 -

Motif 9 width=15
Element Seqn Id St Int Rpt
PLCNENFKKTFKKIL HH1R_RAT 468 23 -
PLCNENFKKTFKRIL HH1R_HUMAN 469 23 -
PLCNENFKKTFKKIL HH1R_BOVIN 473 23 -
PLCNENFRKTFKRIL HH1R_CAVPO 470 23 -
Final Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
SFLWVIPILGWHHF HH1R_RAT 155 155 -
SFLWVIPILGWNHF HH1R_HUMAN 155 155 -
SFLWVIPILGWHHF HH1R_MOUSE 155 155 -
SFLWIIPILGWRHF HH1R_BOVIN 156 156 -
SFLWVIPILGWHHF HH1R_CAVPO 164 164 -

Motif 2 width=19
Element Seqn Id St Int Rpt
CETDFYNVTWFKIMTAIIN HH1R_RAT 180 11 -
CETDFYDVTWFKVMTAIIN HH1R_HUMAN 180 11 -
CETDFYNVTWFKIMTAIIN HH1R_MOUSE 180 11 -
CETDFYNVTWFKVMTAIIN HH1R_BOVIN 181 11 -
CETDFYDVTWFKVMTAIIN HH1R_CAVPO 189 11 -

Motif 3 width=19
Element Seqn Id St Int Rpt
HCQHRQLTNGSLPSFSELK HH1R_RAT 220 21 -
HCQHRELINRSLPSFSEIK HH1R_HUMAN 220 21 -
HCQHPQLTNGSLPTFLEIK HH1R_MOUSE 220 21 -
HCQHRELINGSFPSFSDMK HH1R_BOVIN 221 21 -
HCQHRQLINSSLPSFSEMK HH1R_CAVPO 229 21 -

Motif 4 width=18
Element Seqn Id St Int Rpt
KKPGRESPWGVLKRPSRD HH1R_RAT 249 10 -
KKPGKESPWEVLKRKPKD HH1R_HUMAN 249 10 -
KKPGKESPWGVQTRPSRD HH1R_MOUSE 249 10 -
KKPGKESPWEVLKRKPKD HH1R_BOVIN 250 10 -
RRMGKESPWEDPKRCSKD HH1R_CAVPO 258 10 -

Motif 5 width=14
Element Seqn Id St Int Rpt
EDQTLVDQQSFSRT HH1R_RAT 346 79 -
EDQMLGDSQSFSRT HH1R_HUMAN 348 81 -
EDQTLVDRQSFSRT HH1R_MOUSE 348 81 -
EDQILGDSQSFSRT HH1R_BOVIN 352 84 -
EEWTVVDGQSFPIT HH1R_CAVPO 349 73 -

Motif 6 width=15
Element Seqn Id St Int Rpt
RSGSNSGLDYIKITW HH1R_RAT 376 16 -
RSGSNTGLDYIKFTW HH1R_HUMAN 377 15 -
RRRSNSGLDYIKVTW HH1R_MOUSE 378 16 -
RSESSTGLEYIKFTW HH1R_BOVIN 381 15 -
RSGSNSGLDYIKFTW HH1R_CAVPO 378 15 -

Motif 7 width=18
Element Seqn Id St Int Rpt
WKRLRSHSRQYVSGLHLN HH1R_RAT 390 -1 -
WKRLRSHSRQYVSGLHMN HH1R_HUMAN 391 -1 -
WKRLRSHSRQYVSGLHLN HH1R_MOUSE 392 -1 -
WKRLRSHSRQYVSGLHMN HH1R_BOVIN 395 -1 -
WRRLRSHSRQYTSGLHLN HH1R_CAVPO 392 -1 -

Motif 8 width=12
Element Seqn Id St Int Rpt
FFMVIAFCKSCC HH1R_RAT 433 25 -
FFMVIAFCKNCC HH1R_HUMAN 434 25 -
FFMVIAFCNSCC HH1R_MOUSE 435 25 -
FFMVIAFCESCC HH1R_BOVIN 438 25 -
FFMVIAFCKSCS HH1R_CAVPO 435 25 -

Motif 9 width=15
Element Seqn Id St Int Rpt
PLCNENFKKTFKKIL HH1R_RAT 468 23 -
PLCNENFKKTFKRIL HH1R_HUMAN 469 23 -
PLCNENFKKTFKKIL HH1R_MOUSE 470 23 -
PLCNENFKKTFKKIL HH1R_BOVIN 473 23 -
PLCNENFRKTFKRIL HH1R_CAVPO 470 23 -