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PR00527

Identifier
GASTRINR  [View Relations]  [View Alignment]  
Accession
PR00527
No. of Motifs
9
Creation Date
03-JUN-1996  (UPDATE 10-JUN-1999)
Title
Gastrin receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN

INTERPRO; IPR000314
GCRDB; GCR_0441; GCR_0532; GCR_0769; GCR_0847; GCR_0459
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Cholecystokinin (CCK) and gastrin.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.89-95.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Gastrins and cholecystokinins (CCKs) are naturally-occurring peptides that
share a common C-terminal sequence, GWMDF; full biological activity resides
in this region [6]. The principal physiological role of gastrin is to 
stimulate acid secretion in the stomach; it also has trophic effects on
gastric mucosa [6]. Gastrin is produced from a single gene transcript, and
is found predominantly in the stomach and intestine, but also in vagal
nerves. The CCKB receptor has a widespread distribution in the CNS and
has been implicated in the pathogenesis of panic-anxiety attacks caused
by CCK-related peptides [6]. It has a more limited distribution in the
periphery, where it is found in smooth muscle and secretory glands.
 
GASTRINR is a 9-element fingerprint that provides a signature for the
gastrin (CCKB) receptors. The fingerprint was derived from an initial
alignment of 5 sequences: the motifs were drawn from conserved sections
within either loop or N- and C-terminal regions, focusing on those areas
of the alignment that characterise the gastrin receptors but distinguish
them from the rest of the rhodopsin-like superfamily - motifs 1 and 2 lie
at the N-terminus; motif 3 spans the first external loop; motif 4 spans the
second cytoplasmic loop; motifs 5 and 6 span the second external loop;
motifs 7 and 8 spans the third cytoplasmic loop; and motif 9 lies at the
C-terminus. Two iterations on OWL28.0 were required to reach convergence,
at which point a true set comprising 7 sequences was identified. Several
partial matches were also found, all of which are either gastrin fragments,
or members of the cholecystokinin type A receptor family.
 
An update on SPTR37_9f identified a true set of 7 sequences, and 6
partial matches.
Summary Information
   7 codes involving  9 elements
0 codes involving 8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
1 codes involving 4 elements
4 codes involving 3 elements
1 codes involving 2 elements
Composite Feature Index
9777777777
8000000000
7000000000
6000000000
5000000000
4001101100
3114300030
2000100010
123456789
True Positives
GASR_BOVIN    GASR_CANFA    GASR_HUMAN    GASR_MOUSE    
GASR_PRANA GASR_RABIT GASR_RAT
True Positive Partials
Codes involving 4 elements
CCKR_XENLA
Codes involving 3 elements
CCKR_HUMAN CCKR_RAT O08786 Q63096
Codes involving 2 elements
CCKR_CAVPO
Sequence Titles
GASR_BOVIN  Gastrin/cholecystokinin type B receptor (CCK-B receptor) (CCK-BR) (Cholecystokinin-2 receptor) (CCK2-R) - Bos taurus (Bovine). 
GASR_CANFA Gastrin/cholecystokinin type B receptor (CCK-B receptor) (CCK-BR) (Cholecystokinin-2 receptor) (CCK2-R) - Canis familiaris (Dog).
GASR_HUMAN Gastrin/cholecystokinin type B receptor (CCK-B receptor) (CCK-BR) (Cholecystokinin-2 receptor) (CCK2-R) - Homo sapiens (Human).
GASR_MOUSE Gastrin/cholecystokinin type B receptor (CCK-B receptor) (CCK-BR) (Cholecystokinin-2 receptor) (CCK2-R) - Mus musculus (Mouse).
GASR_PRANA Gastrin/cholecystokinin type B receptor (CCK-B receptor) (CCK-BR) (Cholecystokinin-2 receptor) (CCK2-R) - Praomys natalensis (African soft-furred rat) (Mastomys natalensis).
GASR_RABIT Gastrin/cholecystokinin type B receptor (CCK-B receptor) (CCK-BR) (Cholecystokinin-2 receptor) (CCK2-R) - Oryctolagus cuniculus (Rabbit).
GASR_RAT Gastrin/cholecystokinin type B receptor (CCK-B receptor) (CCK-BR) (Cholecystokinin-2 receptor) (CCK2-R) - Rattus norvegicus (Rat).

CCKR_XENLA Cholecystokinin receptor (CCK-XLR) - Xenopus laevis (African clawed frog).

CCKR_HUMAN Cholecystokinin type A receptor (CCK-A receptor) (CCK-AR) - Homo sapiens (Human).
CCKR_RAT Cholecystokinin type A receptor (CCK-A receptor) (CCK-AR) - Rattus norvegicus (Rat).
O08786 CHOLECYSTOKININ A RECEPTOR (CHOLECYSTOKININ TYPE-A RECEPTOR) - MUS MUSCULUS (MOUSE).
Q63096 CCKB - RATTUS NORVEGICUS (RAT).

CCKR_CAVPO Cholecystokinin type A receptor (CCK-A receptor) (CCK-AR) - Cavia porcellus (Guinea pig).
Scan History
OWL28_0    2  50   NSINGLE    
SPTR37_9f 2 37 NSINGLE
Initial Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
SLCRPGGPLLNNSGTGN GASR_RABIT 18 18 -
SLCHPGVSLLNSSSAGN GASR_PRANA 20 20 -
SLCRPGAPLLNSSSVGN GASR_HUMAN 20 20 -
SLCRAGGALLNSSGAGN GASR_CANFA 20 20 -
SLCRPGVSLLNSSSAGN GASR_RAT 20 20 -

Motif 2 width=17
Element Seqn Id St Int Rpt
LSCEPPRIRGAGTRELE GASR_RABIT 35 0 -
LSCEPPRIRGTGTRELE GASR_PRANA 37 0 -
LSCEPPRIRGAGTRELE GASR_HUMAN 37 0 -
LSCEPPRLRGAGTRELE GASR_CANFA 37 0 -
LSCDPPRIRGTGTRELE GASR_RAT 37 0 -

Motif 3 width=16
Element Seqn Id St Int Rpt
FTLLPNLMGTFIFGTV GASR_RABIT 108 56 -
FTLLPNLMGTFIFGTV GASR_PRANA 110 56 -
FTLLPNLMGTFIFGTV GASR_HUMAN 110 56 -
FTLLPNLMGTFIFGTV GASR_CANFA 110 56 -
FTLLPNLMGTFIFGTV GASR_RAT 110 56 -

Motif 4 width=15
Element Seqn Id St Int Rpt
PLQARVWQTRSHAAR GASR_RABIT 157 33 -
PLQARVWQTRSHAAR GASR_PRANA 159 33 -
PLQARVWQTRSHAAR GASR_HUMAN 159 33 -
PLQARVWQTRSHAAR GASR_CANFA 159 33 -
PLQARVWQTRSHAAR GASR_RAT 159 33 -

Motif 5 width=15
Element Seqn Id St Int Rpt
MVPYPVYTAVQPVGP GASR_RABIT 184 12 -
MVPYPVYTVVQPVGP GASR_PRANA 186 12 -
MVPYPVYTVVQPVGP GASR_HUMAN 186 12 -
MVPYPVYTAVQPAGG GASR_CANFA 186 12 -
MVPYPVYTMVQPVGP GASR_RAT 186 12 -

Motif 6 width=19
Element Seqn Id St Int Rpt
RVLQCVHRWPSARVRQTWS GASR_RABIT 199 0 -
RVLQCMHRWPSARVRQTWS GASR_PRANA 201 0 -
RVLQCVHRWPSARVRQTWS GASR_HUMAN 201 0 -
RALQCVHRWPSARVRQTWS GASR_CANFA 202 1 -
RVLQCMHRWPSARVQQTWS GASR_RAT 201 0 -

Motif 7 width=18
Element Seqn Id St Int Rpt
ISRELYLGLRFDSDSDSE GASR_RABIT 239 21 -
ISRELYLGLRFDGDNDSD GASR_PRANA 241 21 -
ISRELYLGLRFDGDSDSD GASR_HUMAN 241 21 -
ISRELYLGLRFDEDSDSE GASR_CANFA 242 21 -
ISRELYLGLHFDGENDSE GASR_RAT 241 21 -

Motif 8 width=17
Element Seqn Id St Int Rpt
LAGEDGDGCYVQLPRSR GASR_RABIT 288 31 -
VAGEDNDGCYVQLPRSR GASR_PRANA 289 30 -
AVGEDSDGCYVQLPRSR GASR_HUMAN 285 26 -
LAGEDGDGCYVQLPRSR GASR_CANFA 289 29 -
VAGEDSDGCCVQLPRSR GASR_RAT 290 31 -

Motif 9 width=20
Element Seqn Id St Int Rpt
RCCPRPPRARPRPLPDEDPP GASR_RABIT 412 107 -
RCCPRPPRARPRPLPDEDPP GASR_PRANA 410 104 -
RCCPRPPRARPRALPDEDPP GASR_HUMAN 407 105 -
RCCPRPPRARPRPLPDEDPP GASR_CANFA 413 107 -
RCCPRPPRARPQPLPDEDPP GASR_RAT 412 105 -
Final Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
SLCRPGGPLLNNSGTGN GASR_RABIT 18 18 -
SLCHPGVSLLNSSSAGN GASR_PRANA 20 20 -
SLCRSGGPLLNGSGTGN GASR_BOVIN 20 20 -
SLCRPGAPLLNSSSVGN GASR_HUMAN 20 20 -
SLCRAGGALLNSSGAGN GASR_CANFA 20 20 -
SLCRPGVSLLNSSSAGN GASR_MOUSE 20 20 -
SLCRPGVSLLNSSSAGN GASR_RAT 20 20 -

Motif 2 width=17
Element Seqn Id St Int Rpt
LSCEPPRIRGAGTRELE GASR_RABIT 35 0 -
LSCEPPRIRGTGTRELE GASR_PRANA 37 0 -
LSCEPPRIRGAGTRELE GASR_BOVIN 37 0 -
LSCEPPRIRGAGTRELE GASR_HUMAN 37 0 -
LSCEPPRLRGAGTRELE GASR_CANFA 37 0 -
LSCETPRIRGTGTRELE GASR_MOUSE 37 0 -
LSCDPPRIRGTGTRELE GASR_RAT 37 0 -

Motif 3 width=16
Element Seqn Id St Int Rpt
FTLLPNLMGTFIFGTV GASR_RABIT 108 56 -
FTLLPNLMGTFIFGTV GASR_PRANA 110 56 -
FTLLPNLMGTFIFGTV GASR_BOVIN 110 56 -
FTLLPNLMGTFIFGTV GASR_HUMAN 110 56 -
FTLLPNLMGTFIFGTV GASR_CANFA 110 56 -
FTLLPNLMGTFIFGTV GASR_MOUSE 110 56 -
FTLLPNLMGTFIFGTV GASR_RAT 110 56 -

Motif 4 width=15
Element Seqn Id St Int Rpt
PLQARVWQTRSHAAR GASR_RABIT 157 33 -
PLQARVWQTRSHAAR GASR_PRANA 159 33 -
PLQARVWQTRSHAAR GASR_BOVIN 159 33 -
PLQARVWQTRSHAAR GASR_HUMAN 159 33 -
PLQARVWQTRSHAAR GASR_CANFA 159 33 -
PLQARVWQTRSHAAR GASR_MOUSE 159 33 -
PLQARVWQTRSHAAR GASR_RAT 159 33 -

Motif 5 width=15
Element Seqn Id St Int Rpt
MVPYPVYTAVQPVGP GASR_RABIT 184 12 -
MVPYPVYTVVQPVGP GASR_PRANA 186 12 -
MVPYPVYTAVQPAGP GASR_BOVIN 186 12 -
MVPYPVYTVVQPVGP GASR_HUMAN 186 12 -
MVPYPVYTAVQPAGG GASR_CANFA 186 12 -
MVPYPVYTVVQPVGP GASR_MOUSE 186 12 -
MVPYPVYTMVQPVGP GASR_RAT 186 12 -

Motif 6 width=19
Element Seqn Id St Int Rpt
RVLQCVHRWPSARVRQTWS GASR_RABIT 199 0 -
RVLQCMHRWPSARVRQTWS GASR_PRANA 201 0 -
RVLQCMHRWPSARVRQTWS GASR_BOVIN 201 0 -
RVLQCVHRWPSARVRQTWS GASR_HUMAN 201 0 -
RALQCVHRWPSARVRQTWS GASR_CANFA 202 1 -
RILQCMHLWPSERVQQMWS GASR_MOUSE 201 0 -
RVLQCMHRWPSARVQQTWS GASR_RAT 201 0 -

Motif 7 width=18
Element Seqn Id St Int Rpt
ISRELYLGLRFDSDSDSE GASR_RABIT 239 21 -
ISRELYLGLRFDGDNDSD GASR_PRANA 241 21 -
ISRELYLGLRFDGDSDSE GASR_BOVIN 241 21 -
ISRELYLGLRFDGDSDSD GASR_HUMAN 241 21 -
ISRELYLGLRFDEDSDSE GASR_CANFA 242 21 -
ISRELYLGLRFDGDNDSE GASR_MOUSE 241 21 -
ISRELYLGLHFDGENDSE GASR_RAT 241 21 -

Motif 8 width=17
Element Seqn Id St Int Rpt
LAGEDGDGCYVQLPRSR GASR_RABIT 288 31 -
VAGEDNDGCYVQLPRSR GASR_PRANA 289 30 -
LAGEDGDGCYVQLPRSR GASR_BOVIN 290 31 -
AVGEDSDGCYVQLPRSR GASR_HUMAN 285 26 -
LAGEDGDGCYVQLPRSR GASR_CANFA 289 29 -
LTGEDSDGCYVQLPRSR GASR_MOUSE 291 32 -
VAGEDSDGCCVQLPRSR GASR_RAT 290 31 -

Motif 9 width=20
Element Seqn Id St Int Rpt
RCCPRPPRARPRPLPDEDPP GASR_RABIT 412 107 -
RCCPRPPRARPRPLPDEDPP GASR_PRANA 410 104 -
RCCPRPPRARPRPLPDEDPP GASR_BOVIN 414 107 -
RCCPRPPRARPRALPDEDPP GASR_HUMAN 407 105 -
RCCPRPPRARPRPLPDEDPP GASR_CANFA 413 107 -
RCCPRPPRARPRPLPDEDPP GASR_MOUSE 413 105 -
RCCPRPPRARPQPLPDEDPP GASR_RAT 412 105 -