SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR00526

Identifier
FMETLEUPHER  [View Relations]  [View Alignment]  
Accession
PR00526
No. of Motifs
8
Creation Date
21-MAY-1996  (UPDATE 28-JUL-1999)
Title
Formyl-methionyl peptide receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN

INTERPRO; IPR000826
GCRDB; GCR_0069; GCR_0070; GCR_0605
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Formyl-methionyl peptides.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.117-121.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Accumulation of phagocytic cells at sites of injury or infection is
regulated by substances that stimulate chemotaxis, granule secretion,
superoxide generation and up-regulation of cell surface adhesion molecules
in cells of the immune system [6]. The chemoattractant substances include
bacterial N-formyl-methionyl peptides (typified by fMet-Leu-Phe (fMLP)),
many of which participate in pathophysiological conditions, such as
anaphylactic and septic shock [6]. Three fMLP receptors have been
identified: fMLP selectively activates the FPR1 receptor relative to
the FPR2 receptor; its action on FPR3 is unknown. The FPR1 receptor is
found in differentiated myeloid cells (i.e., neutrophils and monocytes),
and in related cell lines; the FPR2 receptor is found only in neutrophils
and monocytes (it binds fMLP at >1000 times higher concentrations than 
at the FPR1 receptor, and may bind other N-formyl-methionyl peptides
with higher affinity) [6].
 
FMETLEUPHER is an 8-element fingerprint that provides a signature for the
fMLP receptors. The fingerprint was derived from an initial alignment of
4 sequences: the motifs were drawn from conserved sections within either
loop or TM regions, focusing on those areas of the alignment that
characterise the fMLP receptors but distinguish them from the rest of the
rhodopsin-like superfamily - motif 1 spans the first cytoplasmic loop;
motif 2 lies in the C-terminal half of TM domain 2; motif 3 lies in the
N-terminal half of TM domain 3; motif 4 spans the second cytoplasmic loop;
motif 5 lies in the second external loop; motif 6 was drawn from TM domain
5; motif 7 spans the third cytoplasmic loop; and motif 8 lies in the
C-terminal region following TM domain 7. Two iterations on OWL27.1 were
required to reach convergence, at which point a true set comprising 8
sequences was identfied. 
 
An update on SPTR37_9f identified a true set of 10 sequences, and 13
partial matches.
Summary Information
  10 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
6 codes involving 3 elements
7 codes involving 2 elements
Composite Feature Index
81010101010101010
700000000
600000000
500000000
400000000
360560001
250060012
12345678
True Positives
FML1_HUMAN    FML1_MOUSE    FML2_HUMAN    FMLR_HUMAN    
FMLR_MOUSE FMLR_RABIT O88535 O88536
O88537 O88538
True Positive Partials
Codes involving 3 elements
C3AR_HUMAN C3AR_MOUSE O43771 O55197
O75388 O88680
Codes involving 2 elements
C5AR_RAT DEZ_HUMAN DEZ_MOUSE DEZ_RAT
MRG_HUMAN O75748 O88416
Sequence Titles
FML1_HUMAN  FMLP-RELATED RECEPTOR I (FMLP-R-I) (RFP) (HM63) (LIPOXIN A4 RECEPTOR) - HOMO SAPIENS (HUMAN). 
FML1_MOUSE FMLP-RELATED RECEPTOR I (FMLP-R-I) (LIPOXIN A4 RECEPTOR) - MUS MUSCULUS (MOUSE).
FML2_HUMAN FMLP-RELATED RECEPTOR II (FMLP-R-II) - HOMO SAPIENS (HUMAN).
FMLR_HUMAN FMET-LEU-PHE RECEPTOR (FMLP RECEPTOR) (N-FORMYL PEPTIDE RECEPTOR) (FPR) - HOMO SAPIENS (HUMAN).
FMLR_MOUSE FMET-LEU-PHE RECEPTOR (FMLP RECEPTOR) (N-FORMYL PEPTIDE RECEPTOR) (FPR) - MUS MUSCULUS (MOUSE).
FMLR_RABIT FMET-LEU-PHE RECEPTOR (FMLP RECEPTOR) (N-FORMYL PEPTIDE RECEPTOR) (FPR) - ORYCTOLAGUS CUNICULUS (RABBIT).
O88535 N-FORMYLPEPTIDE RECEPTOR-LIKE 1 - MUS MUSCULUS (MOUSE).
O88536 N-FORMYLPEPTIDE RECEPTOR-LIKE 2 - MUS MUSCULUS (MOUSE).
O88537 N-FORMYLPEPTIDE RECEPTOR-LIKE 3 - MUS MUSCULUS (MOUSE).
O88538 N-FORMYLPEPTIDE RECEPTOR-LIKE 4 - MUS MUSCULUS (MOUSE).

C3AR_HUMAN C3a anaphylatoxin chemotactic receptor (C3a-R) (C3AR) - Homo sapiens (Human).
C3AR_MOUSE C3a anaphylatoxin chemotactic receptor (C3a-R) (C3AR) (Complement component 3a receptor 1) - Mus musculus (Mouse).
O43771 C3A ANAPHYLATOXIN RECEPTOR - HOMO SAPIENS (HUMAN).
O55197 ANAPHYLATOXIN C3A RECEPTOR - RATTUS NORVEGICUS (RAT).
O75388 ORPHAN G PROTEIN-COUPLED RECEPTOR - HOMO SAPIENS (HUMAN).
O88680 C3A RECEPTOR - CAVIA PORCELLUS (GUINEA PIG).

C5AR_RAT C5a anaphylatoxin chemotactic receptor (C5a-R) (C5aR) (CD88 antigen) - Rattus norvegicus (Rat).
DEZ_HUMAN PROBABLE G PROTEIN-COUPLED RECEPTOR DEZ - HOMO SAPIENS (HUMAN).
DEZ_MOUSE PROBABLE G PROTEIN-COUPLED RECEPTOR DEZ - MUS MUSCULUS (MOUSE).
DEZ_RAT PROBABLE G PROTEIN-COUPLED RECEPTOR DEZ (G-PROTEIN COUPLED CHEMOATTRACTANT-LIKE RECEPTOR) - RATTUS NORVEGICUS (RAT).
MRG_HUMAN MAS-RELATED G PROTEIN-COUPLED RECEPTOR MRG - HOMO SAPIENS (HUMAN).
O75748 ORPHAN G PROTEIN-COUPLED RECEPTOR CHEMR23 - HOMO SAPIENS (HUMAN).
O88416 ORPHAN G PROTEIN-COUPLED RECEPTOR - MUS MUSCULUS (MOUSE).
Scan History
OWL27_1    2  50   NSINGLE    
SPTR37_9f 3 55 NSINGLE
Initial Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
GFRMTRTVNTICYL FML1_HUMAN 52 52 -
GFRMTHTVTTISYL FMLR_HUMAN 52 52 -
GFRMTHTVTTISYL FMLR_RABIT 52 52 -
GFRMTRTVTTICYL FML2_HUMAN 52 52 -

Motif 2 width=17
Element Seqn Id St Int Rpt
FSFSAILPFRMVSVAMR FML1_HUMAN 72 6 -
FCFTSTLPFFMVRKAMG FMLR_HUMAN 72 6 -
FSFTSTLPFFIVTKALG FMLR_RABIT 72 6 -
FSFTATLPFLIVSMAMG FML2_HUMAN 72 6 -

Motif 3 width=18
Element Seqn Id St Int Rpt
KLVHVMIDINLFVSVYLI FML1_HUMAN 99 10 -
KFLFTIVDINLFGSVFLI FMLR_HUMAN 99 10 -
KFVFTIVDINLFGSVFLI FMLR_RABIT 99 10 -
KLIHIVVDINLFGSVFLI FML2_HUMAN 99 10 -

Motif 4 width=17
Element Seqn Id St Int Rpt
CICVLHPAWAQNHRTMS FML1_HUMAN 124 7 -
CVCVLHPVWTQNHRTVS FMLR_HUMAN 124 7 -
CICVLHPVWAQNHRNVS FMLR_RABIT 124 7 -
CICVLHPVWAQNHRTVS FML2_HUMAN 124 7 -

Motif 5 width=15
Element Seqn Id St Int Rpt
CIFNFAFWGDTAVER FML1_HUMAN 176 35 -
CTFNFSPWTNDPKER FMLR_HUMAN 176 35 -
CTFDWSPWTEDPAEK FMLR_RABIT 178 37 -
CTFNFASWGGTPEER FML2_HUMAN 176 35 -

Motif 6 width=14
Element Seqn Id St Int Rpt
KVFLILHFIIGFTV FML1_HUMAN 199 8 -
TVRGIIRFIIGFSA FMLR_HUMAN 199 8 -
MVRGIIRFIIGFST FMLR_RABIT 201 8 -
TARGIIRFVIGFSL FML2_HUMAN 199 8 -

Motif 7 width=18
Element Seqn Id St Int Rpt
IAAKIHRNHMIKSSRPLR FML1_HUMAN 224 11 -
IATKIHKQGLIKSSRPLR FMLR_HUMAN 224 11 -
IATKIHRQGLIKSSRPLR FMLR_RABIT 226 11 -
IAAKIHKKGMIKSSRPLR FML2_HUMAN 224 11 -

Motif 8 width=20
Element Seqn Id St Int Rpt
GRNFQERLIRSLPTSLERAL FML1_HUMAN 306 64 -
GQDFRERLIHALPASLERAL FMLR_HUMAN 305 63 -
GQDFRERLIHSLPASLERAL FMLR_RABIT 307 63 -
GQDFRERLIHSLPTSLERAL FML2_HUMAN 306 64 -
Final Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
GFRMTRTVNTICYL FML1_HUMAN 52 52 -
GFRMPHTVTTIWYL O88536 52 52 -
GFRMTHTVTTISYL FMLR_HUMAN 52 52 -
GFRMKHTVTTISYL FMLR_MOUSE 60 60 -
GFRMTHTVTTISYL FMLR_RABIT 52 52 -
GFRMPHTVTTIWYL FML1_MOUSE 52 52 -
GFRMPHTVTTIWYL O88535 52 52 -
GFRMAHTVTTICYL O88537 52 52 -
GFQMAHTVTTVSYL O88538 52 52 -
GFRMTRTVTTICYL FML2_HUMAN 52 52 -

Motif 2 width=17
Element Seqn Id St Int Rpt
FSFSAILPFRMVSVAMR FML1_HUMAN 72 6 -
FSFTATLPFLLVEMAMK O88536 72 6 -
FCFTSTLPFFMVRKAMG FMLR_HUMAN 72 6 -
FCFTSTLPFYIASMVMG FMLR_MOUSE 80 6 -
FSFTSTLPFFIVTKALG FMLR_RABIT 72 6 -
FSFTATLPFLLVEMAMK FML1_MOUSE 72 6 -
FSFTATLPFLLVEMAMK O88535 72 6 -
FSFMVTLPLHIISMVMK O88537 72 6 -
LSFMVTLPLHIISMVMR O88538 72 6 -
FSFTATLPFLIVSMAMG FML2_HUMAN 72 6 -

Motif 3 width=18
Element Seqn Id St Int Rpt
KLVHVMIDINLFVSVYLI FML1_HUMAN 99 10 -
KLVHIVVDVNLFGSVFLI O88536 99 10 -
KFLFTIVDINLFGSVFLI FMLR_HUMAN 99 10 -
KFIYTVIDINLFGSVFLI FMLR_MOUSE 107 10 -
KFVFTIVDINLFGSVFLI FMLR_RABIT 99 10 -
KLVHIAVDVNLFGSVFLI FML1_MOUSE 99 10 -
KLVHIAVDVNLFGSVFLI O88535 99 10 -
KFVLSIVHINLFVSVFLI O88537 99 10 -
KLVHIIANINLFVSIFLI O88538 99 10 -
KLIHIVVDINLFGSVFLI FML2_HUMAN 99 10 -

Motif 4 width=17
Element Seqn Id St Int Rpt
CICVLHPAWAQNHRTMS FML1_HUMAN 124 7 -
CICVLHPVWAQNHRTVS O88536 124 7 -
CVCVLHPVWTQNHRTVS FMLR_HUMAN 124 7 -
CICVLHPVWAQNHRTVS FMLR_MOUSE 132 7 -
CICVLHPVWAQNHRNVS FMLR_RABIT 124 7 -
CICVLHPVWAQNHRTVS FML1_MOUSE 124 7 -
CICVLHPVWAQNHRTVS O88535 124 7 -
CTCVLHPVWVQNHRTVS O88537 124 7 -
CICVLCPVWSQNHRTVS O88538 124 7 -
CICVLHPVWAQNHRTVS FML2_HUMAN 124 7 -

Motif 5 width=15
Element Seqn Id St Int Rpt
CIFNFAFWGDTAVER FML1_HUMAN 176 35 -
CTFNFGSWAQTDEEK O88536 176 35 -
CTFNFSPWTNDPKER FMLR_HUMAN 176 35 -
CTFDFSPWTKDPVEK FMLR_MOUSE 187 38 -
CTFDWSPWTEDPAEK FMLR_RABIT 178 37 -
CRLSFVSWGNSVEER FML1_MOUSE 176 35 -
CRLSFVSWGNSVEER O88535 172 31 -
CTCNFESVVANPEEQ O88537 176 35 -
CISKFESWVATSEEQ O88538 176 35 -
CTFNFASWGGTPEER FML2_HUMAN 176 35 -

Motif 6 width=14
Element Seqn Id St Int Rpt
KVFLILHFIIGFTV FML1_HUMAN 199 8 -
TTRGIIRFLIGFSM O88536 199 8 -
TVRGIIRFIIGFSA FMLR_HUMAN 199 8 -
TVRGIIRFIIGFST FMLR_MOUSE 210 8 -
MVRGIIRFIIGFST FMLR_RABIT 201 8 -
TTRGIIRFIVSFSL FML1_MOUSE 199 8 -
TTRGIIRFIVSFSL O88535 195 8 -
TATGIISFINCFSL O88537 199 8 -
TASGIINFIIGFSM O88538 199 8 -
TARGIIRFVIGFSL FML2_HUMAN 199 8 -

Motif 7 width=18
Element Seqn Id St Int Rpt
IAAKIHRNHMIKSSRPLR FML1_HUMAN 224 11 -
IAVKINRRNLVNSSRPLR O88536 224 11 -
IATKIHKQGLIKSSRPLR FMLR_HUMAN 224 11 -
ITTKIHRQGLIKSSRPLR FMLR_MOUSE 235 11 -
IATKIHRQGLIKSSRPLR FMLR_RABIT 226 11 -
ITTKIHKKAFVNSSRPFR FML1_MOUSE 224 11 -
ITYKIHKKAFVNSSRPSR O88535 220 11 -
MAAKICRKGFLNSSRPLR O88537 224 11 -
MAAKICRRGFVNSSRPLR O88538 224 11 -
IAAKIHKKGMIKSSRPLR FML2_HUMAN 224 11 -

Motif 8 width=20
Element Seqn Id St Int Rpt
GRNFQERLIRSLPTSLERAL FML1_HUMAN 306 64 -
GQDFRERFIHSLPYSLERAL O88536 306 64 -
GQDFRERLIHALPASLERAL FMLR_HUMAN 305 63 -
GQDFRERLIHSLPASLERAL FMLR_MOUSE 316 63 -
GQDFRERLIHSLPASLERAL FMLR_RABIT 307 63 -
GQDFQERLIHSLSSRLQRAL FML1_MOUSE 306 64 -
GQDFQERLIHSLSSRLPRAL O88535 302 64 -
GQEFREKLIYSLSASLERAL O88537 301 59 -
GQEFRDRLIYSLYASLERAL O88538 301 59 -
GQDFRERLIHSLPTSLERAL FML2_HUMAN 306 64 -