| Identifier | DELTAOPIOIDR [View Relations] [View Alignment]
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| Accession | PR00525 |
| No. of Motifs | 6 |
| Creation Date | 19-MAY-1996 (UPDATE 07-JUN-1999) |
| Title | Delta opioid receptor signature |
| Database References | PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR00384 OPIOIDR
INTERPRO; IPR000321
GCRDB; GCR_0229; GCR_0493; GCR_0634; GCR_0842 |
| Literature References | 1. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
2. ATTWOOD, T.K. AND FINDLAY, J.B.C.
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
5. ATTWOOD, T.K. AND FINDLAY, J.B.C.
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
6. WATSON, S. AND ARKINSTALL, S.
Opioid peptides.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.205-213.
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| Documentation | G protein-coupled receptors (GPCRs) constitute a vast protein family that
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the
sequence level, on the basis of which they can be separated into distinct
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship,
but between which there is no statistically significant similarity in
sequence [1]. The currently known clan members include the rhodopsin-like
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
The rhodopsin-like GPCRs themselves represent a widespread protein family
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary
widely in structure and character, the amino acid sequences of the
receptors are very similar and are believed to adopt a common structural
framework comprising 7 transmembrane (TM) helices [3-5].
The term opioid refers to a class of substance that produces its effects
via the major classes of opioid receptor, termed mu, delta and kappa [6].
The delta opioid receptor has a more discrete distribution in the CNS
relative to the mu and kappa opioid receptors: it is found in the cerebral
cortex, amygdala, nucleus accumbens, olfactory tubercle and pontine
nucleus. It is also found in certain smooth muscles, e.g. hamster vas
deferens, and in cell lines [6]. Delta-receptors mediate analgesia.
DELTAOPIOIDR is a 6-element fingerprint that provides a signature for the
delta opioid receptors. The fingerprint was derived from an initial
alignment of 3 sequences: the motifs were drawn from conserved sections
within either loop or N- and C-terminal regions, focusing on those areas
of the alignment that characterise the delta opioid receptors but
distinguish them from the rest of the opioid receptor family - motifs 1
and 2 were drawn from the N-terminal region preceding TM domain 1; motifs
3 and 4 lie in the second and third external loops; and motifs 5 and 6 were
drawn from the C-terminal region following TM domain 7. Two iterations on
OWL27.1 were required to reach convergence, at which point a true set
comprising 4 sequences was identified.
An update on SPTR37_9f identified a true set of 3 sequences.
|
| Summary Information | 3 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
|
| Composite Feature Index | | 6 | 3 | 3 | 3 | 3 | 3 | 3 | | 5 | 0 | 0 | 0 | 0 | 0 | 0 | | 4 | 0 | 0 | 0 | 0 | 0 | 0 | | 3 | 0 | 0 | 0 | 0 | 0 | 0 | | 2 | 0 | 0 | 0 | 0 | 0 | 0 | | 1 | 2 | 3 | 4 | 5 | 6 |
|
| True Positives | OPRD_HUMAN OPRD_MOUSE OPRD_RAT |
| Sequence Titles | OPRD_HUMAN Delta-type opioid receptor (DOR-1) - Homo sapiens (Human).
OPRD_MOUSE Delta-type opioid receptor (DOR-1) (K56) (MSL-2) - Mus musculus (Mouse).
OPRD_RAT Delta-type opioid receptor (DOR-1) (Opioid receptor A) - Rattus norvegicus (Rat).
|
| Scan History | OWL27_1 2 50 NSINGLE
SPTR37_9f 2 4 NSINGLE
|
| Initial Motifs | Motif 1 width=14
Element Seqn Id St Int Rpt
NLSDAFPSAFPSAG OPRD_MOUSE 18 18 -
NVSDTFPSAFPSAS OPRD_RAT 18 18 -
NASDAYPSAFPSAG OPRD_HUMAN 18 18 -
Motif 2 width=15
Element Seqn Id St Int Rpt
ANASGSPGARSASSL OPRD_MOUSE 32 0 -
ANASGSPGARSASSL OPRD_RAT 32 0 -
ANASGPPGPGSASSL OPRD_HUMAN 32 0 -
Motif 3 width=13
Element Seqn Id St Int Rpt
AVTQPRDGAVVCM OPRD_MOUSE 187 140 -
AVTQPRDGAVVCT OPRD_RAT 187 140 -
AVTRPRDGAVVCM OPRD_HUMAN 187 140 -
Motif 4 width=14
Element Seqn Id St Int Rpt
WTLVDINRRDPLVV OPRD_MOUSE 284 84 -
WTLVDINRRDPLVV OPRD_RAT 284 84 -
WTLVDIDRRDPLVV OPRD_HUMAN 284 84 -
Motif 5 width=13
Element Seqn Id St Int Rpt
RTPCGRQEPGSLR OPRD_MOUSE 334 36 -
RAPCGGQEPGSLR OPRD_RAT 334 36 -
RKPCGRPDPSSFS OPRD_HUMAN 334 36 -
Motif 6 width=19
Element Seqn Id St Int Rpt
ATTRERVTACTPSDGPGGG OPRD_MOUSE 351 4 -
ATARERVTACTPSDGPGGG OPRD_RAT 351 4 -
ATARERVTACTPSDGPGGG OPRD_HUMAN 351 4 -
|
| Final Motifs | Motif 1 width=14
Element Seqn Id St Int Rpt
NLSDAFPSAFPSAG OPRD_MOUSE 18 18 -
NVSDTFPSAFPSAS OPRD_RAT 18 18 -
NASDAYPSAFPSAG OPRD_HUMAN 18 18 -
Motif 2 width=15
Element Seqn Id St Int Rpt
ANASGSPGARSASSL OPRD_MOUSE 32 0 -
ANASGSPGARSASSL OPRD_RAT 32 0 -
ANASGPPGPGSASSL OPRD_HUMAN 32 0 -
Motif 3 width=13
Element Seqn Id St Int Rpt
AVTQPRDGAVVCM OPRD_MOUSE 187 140 -
AVTQPRDGAVVCT OPRD_RAT 187 140 -
AVTRPRDGAVVCM OPRD_HUMAN 187 140 -
Motif 4 width=14
Element Seqn Id St Int Rpt
WTLVDINRRDPLVV OPRD_MOUSE 284 84 -
WTLVDINRRDPLVV OPRD_RAT 284 84 -
WTLVDIDRRDPLVV OPRD_HUMAN 284 84 -
Motif 5 width=13
Element Seqn Id St Int Rpt
RTPCGRQEPGSLR OPRD_MOUSE 334 36 -
RAPCGGQEPGSLR OPRD_RAT 334 36 -
RKPCGRPDPSSFS OPRD_HUMAN 334 36 -
Motif 6 width=19
Element Seqn Id St Int Rpt
ATTRERVTACTPSDGPGGG OPRD_MOUSE 351 4 -
ATARERVTACTPSDGPGGG OPRD_RAT 351 4 -
ATARERVTACTPSDGPGGG OPRD_HUMAN 351 4 -
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