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PR00525

Identifier
DELTAOPIOIDR  [View Relations]  [View Alignment]  
Accession
PR00525
No. of Motifs
6
Creation Date
19-MAY-1996  (UPDATE 07-JUN-1999)
Title
Delta opioid receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR00384 OPIOIDR
INTERPRO; IPR000321
GCRDB; GCR_0229; GCR_0493; GCR_0634; GCR_0842
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Opioid peptides.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.205-213.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
The term opioid refers to a class of substance that produces its effects
via the major classes of opioid receptor, termed mu, delta and kappa [6].
The delta opioid receptor has a more discrete distribution in the CNS
relative to the mu and kappa opioid receptors: it is found in the cerebral
cortex, amygdala, nucleus accumbens, olfactory tubercle and pontine
nucleus. It is also found in certain smooth muscles, e.g. hamster vas
deferens, and in cell lines [6]. Delta-receptors mediate analgesia.
 
DELTAOPIOIDR is a 6-element fingerprint that provides a signature for the 
delta opioid receptors. The fingerprint was derived from an initial
alignment of 3 sequences: the motifs were drawn from conserved sections
within either loop or N- and C-terminal regions, focusing on those areas
of the alignment that characterise the delta opioid receptors but
distinguish them from the rest of the opioid receptor family - motifs 1
and 2 were drawn from the N-terminal region preceding TM domain 1; motifs
3 and 4 lie in the second and third external loops; and motifs 5 and 6 were
drawn from the C-terminal region following TM domain 7. Two iterations on
OWL27.1 were required to reach convergence, at which point a true set
comprising 4 sequences was identified.
 
An update on SPTR37_9f identified a true set of 3 sequences.
Summary Information
3 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6333333
5000000
4000000
3000000
2000000
123456
True Positives
OPRD_HUMAN    OPRD_MOUSE    OPRD_RAT      
Sequence Titles
OPRD_HUMAN  Delta-type opioid receptor (DOR-1) - Homo sapiens (Human). 
OPRD_MOUSE Delta-type opioid receptor (DOR-1) (K56) (MSL-2) - Mus musculus (Mouse).
OPRD_RAT Delta-type opioid receptor (DOR-1) (Opioid receptor A) - Rattus norvegicus (Rat).
Scan History
OWL27_1    2  50   NSINGLE    
SPTR37_9f 2 4 NSINGLE
Initial Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
NLSDAFPSAFPSAG OPRD_MOUSE 18 18 -
NVSDTFPSAFPSAS OPRD_RAT 18 18 -
NASDAYPSAFPSAG OPRD_HUMAN 18 18 -

Motif 2 width=15
Element Seqn Id St Int Rpt
ANASGSPGARSASSL OPRD_MOUSE 32 0 -
ANASGSPGARSASSL OPRD_RAT 32 0 -
ANASGPPGPGSASSL OPRD_HUMAN 32 0 -

Motif 3 width=13
Element Seqn Id St Int Rpt
AVTQPRDGAVVCM OPRD_MOUSE 187 140 -
AVTQPRDGAVVCT OPRD_RAT 187 140 -
AVTRPRDGAVVCM OPRD_HUMAN 187 140 -

Motif 4 width=14
Element Seqn Id St Int Rpt
WTLVDINRRDPLVV OPRD_MOUSE 284 84 -
WTLVDINRRDPLVV OPRD_RAT 284 84 -
WTLVDIDRRDPLVV OPRD_HUMAN 284 84 -

Motif 5 width=13
Element Seqn Id St Int Rpt
RTPCGRQEPGSLR OPRD_MOUSE 334 36 -
RAPCGGQEPGSLR OPRD_RAT 334 36 -
RKPCGRPDPSSFS OPRD_HUMAN 334 36 -

Motif 6 width=19
Element Seqn Id St Int Rpt
ATTRERVTACTPSDGPGGG OPRD_MOUSE 351 4 -
ATARERVTACTPSDGPGGG OPRD_RAT 351 4 -
ATARERVTACTPSDGPGGG OPRD_HUMAN 351 4 -
Final Motifs
Motif 1  width=14
Element Seqn Id St Int Rpt
NLSDAFPSAFPSAG OPRD_MOUSE 18 18 -
NVSDTFPSAFPSAS OPRD_RAT 18 18 -
NASDAYPSAFPSAG OPRD_HUMAN 18 18 -

Motif 2 width=15
Element Seqn Id St Int Rpt
ANASGSPGARSASSL OPRD_MOUSE 32 0 -
ANASGSPGARSASSL OPRD_RAT 32 0 -
ANASGPPGPGSASSL OPRD_HUMAN 32 0 -

Motif 3 width=13
Element Seqn Id St Int Rpt
AVTQPRDGAVVCM OPRD_MOUSE 187 140 -
AVTQPRDGAVVCT OPRD_RAT 187 140 -
AVTRPRDGAVVCM OPRD_HUMAN 187 140 -

Motif 4 width=14
Element Seqn Id St Int Rpt
WTLVDINRRDPLVV OPRD_MOUSE 284 84 -
WTLVDINRRDPLVV OPRD_RAT 284 84 -
WTLVDIDRRDPLVV OPRD_HUMAN 284 84 -

Motif 5 width=13
Element Seqn Id St Int Rpt
RTPCGRQEPGSLR OPRD_MOUSE 334 36 -
RAPCGGQEPGSLR OPRD_RAT 334 36 -
RKPCGRPDPSSFS OPRD_HUMAN 334 36 -

Motif 6 width=19
Element Seqn Id St Int Rpt
ATTRERVTACTPSDGPGGG OPRD_MOUSE 351 4 -
ATARERVTACTPSDGPGGG OPRD_RAT 351 4 -
ATARERVTACTPSDGPGGG OPRD_HUMAN 351 4 -