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PR00523

Identifier
CANABINOID2R  [View Relations]  [View Alignment]  
Accession
PR00523
No. of Motifs
5
Creation Date
19-MAY-1996  (UPDATE 06-JUN-1999)
Title
Cannabinoid receptor type 2 signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR00362 CANNABINOIDR
INTERPRO; IPR001551
GCRDB; GCR_0645
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Cannabinoid.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.80-83.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
The ability of marijuana to activate the cannabinoid receptor provides a
molecular explanation for its psychoactive effects and other CNS actions
(which include hallucinations, memory deficits, altered time and space
perception, CNS depression and appetite stimulation) [6]. The endogenous
ligand at the cannabinoid receptor is unknown, although it may be a
derivative of arachidonic acid. The cannabinoid receptor is widespread
throughout the CNS, high levels occurring in the dendate gyrus, hippocampus
and cerebral cortex, with more moderate levels in the hypothalamus and
amygdala. It is also present in various cell lines, and in the periphery
it is found in the testis and vas deferens [6].
 
CANABINOID2R is a 5-element fingerprint that provides a signature for type
2 cannabinoid receptors. The fingerprint was derived from an initial
alignment of 3 sequences: the motifs were drawn from conserved sections   
within either loop or N- and C-terminal regions, focusing on those areas
of the alignment that characterise type 2 cannabinoid receptors but
distinguish them from the rest of the cannabinoid receptor family - motif 1
lies in the N-terminal region immediately preceding TM domain 1; motif 2
spans the third cytoplasmic loop; motif 3 spans the third external loop;
and motifs 4 and 5 lie in the C-terminal region following TM domain 7. A
single iteration on OWL27.1 was required to reach convergence, no further
sequences being identified beyond the starting set.
 
An update on SPTR37_9f identified a true set of 2 sequences.
Summary Information
2 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
522222
400000
300000
200000
12345
True Positives
CB2R_HUMAN    CB2R_MOUSE    
Sequence Titles
CB2R_HUMAN  CANNABINOID RECEPTOR 2 (CB2) (CB-2) (CX5) - HOMO SAPIENS (HUMAN). 
CB2R_MOUSE CANNABINOID RECEPTOR 2 (CB2) (CB-2) - MUS MUSCULUS (MOUSE).
Scan History
OWL27_1    1  100  NSINGLE    
SPTR37_9f 2 3 NSINGLE
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
TEVTNGSNGGLEFNPMKEY MMU21681 7 7 -
TEVTNGSNGGLEFNPMKEY S54163 7 7 -
TEIANGSKDGLDSNPMKDY CB2R_HUMAN 7 7 -

Motif 2 width=18
Element Seqn Id St Int Rpt
VASLSGHQDRQVPGMARM CB2R_HUMAN 220 194 -
VATLAEHQDRQVPGIARM MMU21681 220 194 -
VATLAEHQDRQVPGIARM S54163 220 194 -

Motif 3 width=17
Element Seqn Id St Int Rpt
LMAHSLATTLSDQVKKA CB2R_HUMAN 264 26 -
LMGHSLVTTLSDQVKEA S54163 264 26 -
LMGHSLVTMLSDQVKEA MMU21681 264 26 -

Motif 4 width=18
Element Seqn Id St Int Rpt
RSAAQHCLIGWKKYLQGL MMU21681 307 26 -
RSAAQHCLIGWKKYLQGL S54163 307 26 -
RSSAHHCLAHWKKCVRGL CB2R_HUMAN 307 26 -

Motif 5 width=19
Element Seqn Id St Int Rpt
GPEGKEEGPRSSVTETEAD S54163 325 0 -
GSEAKEEAPRSSVTETEAD CB2R_HUMAN 325 0 -
GPEGKEEAPRSSVTETEAD MMU21681 325 0 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
TEIANGSKDGLDSNPMKDY CB2R_HUMAN 7 7 -
TEVTNGSNGGLEFNPMKEY CB2R_MOUSE 7 7 -

Motif 2 width=18
Element Seqn Id St Int Rpt
VASLSGHQDRQVPGMARM CB2R_HUMAN 220 194 -
VATLAEHQDRQVPGIARM CB2R_MOUSE 220 194 -

Motif 3 width=17
Element Seqn Id St Int Rpt
LMAHSLATTLSDQVKKA CB2R_HUMAN 264 26 -
LMGHSLVTTLSDQVKEA CB2R_MOUSE 264 26 -

Motif 4 width=18
Element Seqn Id St Int Rpt
RSSAHHCLAHWKKCVRGL CB2R_HUMAN 307 26 -
RSAAQHCLIGWKKYLQGL CB2R_MOUSE 307 26 -

Motif 5 width=19
Element Seqn Id St Int Rpt
GSEAKEEAPRSSVTETEAD CB2R_HUMAN 325 0 -
GPEGKEEGPRSSVTETEAD CB2R_MOUSE 325 0 -