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PR00522

Identifier
CANABINOID1R  [View Relations]  [View Alignment]  
Accession
PR00522
No. of Motifs
7
Creation Date
19-MAY-1996  (UPDATE 06-JUN-1999)
Title
Cannabinoid receptor type 1 signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR00362 CANNABINOIDR
INTERPRO; IPR000810
GCRDB; GCR_0290; GCR_0055
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Cannabinoid.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.80-83.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
The ability of marijuana to activate the cannabinoid receptor provides a
molecular explanation for its psychoactive effects and other CNS actions
(which include hallucinations, memory deficits, altered time and space
perception, CNS depression and appetite stimulation) [6]. The endogenous
ligand at the cannabinoid receptor is unknown, although it may be a
derivative of arachidonic acid. The cannabinoid receptor is widespread
throughout the CNS, high levels occurring in the dendate gyrus, hippocampus
and cerebral cortex, with more moderate levels in the hypothalamus and
amygdala. It is also present in various cell lines, and in the periphery
it is found in the testis and vas deferens [6].
 
CANABINOID1R is a 7-element fingerprint that provides a signature for type
1 cannabinoid receptors. The fingerprint was derived from an initial
alignment of 3 sequences: the motifs were drawn from conserved sections   
within either loop or N- and C-terminal regions, focusing on those areas
of the alignment that characterise the type 1 cannabinoid receptors but
distinguish them from the rest of the cannabinoid receptor family - motifs
1-3 lie in the N-terminal region immediately preceding TM domain 1; motif
4 lies in the third cytoplasmic loop; motif 5 spans the third external
loop; and motifs 6 and 7 lie in the C-terminal region following TM domain
7. A single iteration on OWL27.1 was required to reach convergence, no
further sequences being identified beyond the starting set. 
 
An update on SPTR37_9f identified a true set of 4 sequences, and 3
partial matches.
Summary Information
   4 codes involving  7 elements
0 codes involving 6 elements
1 codes involving 5 elements
2 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
74444444
60000000
50011111
42001221
30000000
20000000
1234567
True Positives
CB1R_FELCA    CB1R_HUMAN    CB1R_MOUSE    CB1R_RAT      
True Positive Partials
Codes involving 5 elements
Q13949
Codes involving 4 elements
CB1A_FUGRU CB1B_FUGRU
Sequence Titles
CB1R_FELCA  CANNABINOID RECEPTOR 1 (CB1) (CB-R) - FELIS SILVESTRIS CATUS (CAT). 
CB1R_HUMAN CANNABINOID RECEPTOR 1 (CB1) (CB-R) (CANN6) - HOMO SAPIENS (HUMAN).
CB1R_MOUSE CANNABINOID RECEPTOR 1 (CB1) (CB-R) (BRAIN-TYPE CANNABINOID RECEPTOR) - MUS MUSCULUS (MOUSE).
CB1R_RAT CANNABINOID RECEPTOR 1 (CB1) (CB-R) (BRAIN-TYPE CANNABINOID RECEPTOR) - RATTUS NORVEGICUS (RAT).

Q13949 CENTRAL CANNABINOID RECEPTOR - HOMO SAPIENS (HUMAN).

CB1A_FUGRU CANNABINOID RECEPTOR TYPE 1A - Fugu rubripes (Japanese pufferfish) (Takifugu rubripes).
CB1B_FUGRU CANNABINOID RECEPTOR TYPE 1B - FUGU RUBRIPES (JAPANESE PUFFERFISH) (TAKIFUGU RUBRIPES).
Scan History
OWL27_1    1  100  NSINGLE    
SPTR37_9f 2 23 NSINGLE
Initial Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
FRTITTDLLYVGSNDIQYED CB1R_HUMAN 13 13 -
FRTITTDLLYVGSNDIQYED CB1R_RAT 13 13 -
FRTITTDLLYVGSNDIQYED MMU17985 13 13 -

Motif 2 width=23
Element Seqn Id St Int Rpt
DMASKLGYFPQKFPLTSFRGSPF CB1R_HUMAN 36 3 -
DMASKLGYFPQKFPLTSFRGSPF MMU17985 36 3 -
DMASKLGYFPQKFPLTSFRGSPF CB1R_RAT 36 3 -

Motif 3 width=20
Element Seqn Id St Int Rpt
TEFYNKSLSSFKENEENIQC CB1R_RAT 80 21 -
TEFYNKSLSSFKENEDNIQC MMU17985 80 21 -
TEFYNKSLSSFKENEENIQC CB1R_HUMAN 79 20 -

Motif 4 width=20
Element Seqn Id St Int Rpt
RMIQRGTQKSIIIHTSEDGK CB1R_HUMAN 307 208 -
RMIQRGTQKSIIIHTSEDGK MMU17985 308 208 -
RMIQRGTQKSIIIHTSEDGK CB1R_RAT 308 208 -

Motif 5 width=12
Element Seqn Id St Int Rpt
VYDVFGKMNKLI MMU17985 365 37 -
VYDVFGKMNKLI CB1R_RAT 365 37 -
VYDVFGKMNKLI CB1R_HUMAN 364 37 -

Motif 6 width=18
Element Seqn Id St Int Rpt
HAFRSMFPSCEGTAQPLD MMU17985 407 30 -
HAFRSMFPSCEGTAQPLD CB1R_RAT 407 30 -
HAFRSMFPSCEGTAQPLD CB1R_HUMAN 406 30 -

Motif 7 width=20
Element Seqn Id St Int Rpt
HKHANNTASMHRAAESCIKS MMU17985 434 9 -
HKHANNAASVHRAAESCIKS CB1R_HUMAN 433 9 -
HKHANNTASMHRAAESCIKS CB1R_RAT 434 9 -
Final Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
FRTITTDLLYVGSNDIQYED CB1R_RAT 13 13 -
FRTITTDLLYVGSNDIQYED CB1R_MOUSE 13 13 -
FRTITTDLLYVGSNDIQYED CB1R_HUMAN 13 13 -
FRTITTDLLYVGSNDIQYED CB1R_FELCA 13 13 -

Motif 2 width=23
Element Seqn Id St Int Rpt
DMASKLGYFPQKFPLTSFRGSPF CB1R_RAT 36 3 -
DMASKLGYFPQKFPLTSFRGSPF CB1R_MOUSE 36 3 -
DMASKLGYFPQKFPLTSFRGSPF CB1R_HUMAN 36 3 -
DMASKLGYFPQKFPLTSFRGSPF CB1R_FELCA 36 3 -

Motif 3 width=20
Element Seqn Id St Int Rpt
TEFYNKSLSSFKENEENIQC CB1R_RAT 80 21 -
TEFYNKSLSSFKENEDNIQC CB1R_MOUSE 80 21 -
TEFYNKSLSSFKENEENIQC CB1R_HUMAN 79 20 -
TEFYNKSLSSYKENEENIQC CB1R_FELCA 79 20 -

Motif 4 width=20
Element Seqn Id St Int Rpt
RMIQRGTQKSIIIHTSEDGK CB1R_RAT 308 208 -
RMIQRGTQKSIIIHTSEDGK CB1R_MOUSE 308 208 -
RMIQRGTQKSIIIHTSEDGK CB1R_HUMAN 307 208 -
RMIQRGTQKSIIIHTSEDGK CB1R_FELCA 307 208 -

Motif 5 width=12
Element Seqn Id St Int Rpt
VYDVFGKMNKLI CB1R_RAT 365 37 -
VYDVFGKMNKLI CB1R_MOUSE 365 37 -
VYDVFGKMNKLI CB1R_HUMAN 364 37 -
VYDVFGKMNKLI CB1R_FELCA 364 37 -

Motif 6 width=18
Element Seqn Id St Int Rpt
HAFRSMFPSCEGTAQPLD CB1R_RAT 407 30 -
HAFRSMFPSCEGTAQPLD CB1R_MOUSE 407 30 -
HAFRSMFPSCEGTAQPLD CB1R_HUMAN 406 30 -
HAFRSMFPSCEGTAQPLD CB1R_FELCA 406 30 -

Motif 7 width=20
Element Seqn Id St Int Rpt
HKHANNTASMHRAAESCIKS CB1R_RAT 434 9 -
HKHANNTASMHRAAESCIKS CB1R_MOUSE 434 9 -
HKHANNAASVHRAAESCIKS CB1R_HUMAN 433 9 -
HKHANNTANVHRAAENCIKN CB1R_FELCA 433 9 -