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PR00510

Identifier
NEBULIN  [View Relations]  [View Alignment]  
Accession
PR00510
No. of Motifs
6
Creation Date
01-JUN-1996  (UPDATE 07-JUN-1999)
Title
Nebulin signature
Database References

PFAM; PF00880 Nebulin_repeat
INTERPRO; IPR000900
Literature References
1. PFUHL, M., WINDER, S.J., CASTIGLIONE MORELLI, M.A., LABEIT, S. 
AND PASTORE, A.
Correlation between conformational and binding properties of nebulin
repeats.
J.MOL.BIOL. 257 367-384 (1996). 

Documentation
Nebulin is a 600-800kDa protein found in the thin filaments of striated
vertebrate muscle. It is presumed to play a role in binding and stabilising
F-actin [1], essentially by providing a template for actin polymerisation 
(i.e., acting as an "actin zipper"). The amino acid sequence shows a
uniform repeating pattern along its length, a repeated 35-residue motif
constituting up to 97% of the polypeptide. Analysis of individual repeats
reveals a progressive N- to C-terminal divergence, coupled with an
increasing alpha-helix propensity [1]. This correlates with a higher
binding affinity for F-actin at the C-terminus. Thus, it is postulated
that once the repeats have formed an initiation complex, the whole length
of the nebulin molecule may then associate in a highly co-operative process
with the thin filament, in a manner similar to the closing of a zipper [1].
 
NEBULIN is a 6-element fingerprint that provides a signature for nebulins.
The fingerprint was derived from an initial alignment of 3 sequences: the
motifs were drawn from short conserved regions spanning 3 repeats in the
central portion of the alignment. Two iterations on OWL28.0 were required
to reach convergence, at which point a true set comprising 7 sequences was
identified. Two partial matches were also found (OCNBLNP and BOVNBLNA), 
both of which are fragments. 
 
An update on SPTR37_9f identified a true set of 2 sequences.
Summary Information
2 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6222222
5000000
4000000
3000000
2000000
123456
True Positives
O35884        Q15346        
Sequence Titles
O35884      NEBULIN-RELATED ANCHORING PROTEIN (N-RAP) - MUS MUSCULUS (MOUSE). 
Q15346 NEBULIN - HOMO SAPIENS (HUMAN).
Scan History
OWL28_0    2  50   NSINGLE    
SPTR37_9f 2 18 NSINGLE
Initial Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
SRNIASDCKYKEAYEKAKGK HSNBLNP 167 167 -
SRDIASDYKYKEAYEKQKGH S55025 27 27 -
SRDIASDYKYKKAYEQAKGK NEBU_HUMAN 92 92 -

Motif 2 width=20
Element Seqn Id St Int Rpt
DDPKLVHYMNVAKIQSDREY HSNBLNP 195 8 -
DDPKIMCAIHAEKIQSEREY S55025 55 8 -
DDPKLVHFMQVAKMQSDREY NEBU_HUMAN 120 8 -

Motif 3 width=20
Element Seqn Id St Int Rpt
KKGYEKSKTSFHTPVDMLSV HSNBLNP 459 244 -
KKEFQKWKTKFSSPVDMLSI S55025 75 0 -
KKGYEKSKTSFHTPVDMLSV NEBU_HUMAN 140 0 -

Motif 4 width=16
Element Seqn Id St Int Rpt
AAKKSQEVATNANYRN HSNBLNP 480 1 -
LAKKCQTLVTDIYYRN S55025 96 1 -
AAKKSQEVATNANYRN NEBU_HUMAN 161 1 -

Motif 5 width=19
Element Seqn Id St Int Rpt
HTYNMLPDAMSFELAKNMM HSNBLNP 498 2 -
HEWTCMPDQNDIIQAKKAY S55025 114 2 -
HTYNMLPDAMSFELAKNMM NEBU_HUMAN 179 2 -

Motif 6 width=17
Element Seqn Id St Int Rpt
FMKGIGWLPLGSLEAEK HSNBLNP 531 14 -
WLRGIGWMPQGSPEVLR S55025 146 13 -
FMKGIGWLLWLPGSREK NEBU_HUMAN 212 14 -
Final Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
SRDIASDYKYKEGYRKQLGH Q15346 3514 3514 R6
SRDIASEFKYKEGYRKQLGH Q15346 2785 2785 R3
SRDVISDYKYKDGYRKQLGH Q15346 3028 3028 R4
SREIASEYKYKEGFRKQLGH Q15346 2542 2542 R2
SRDIASDYKYKQGYRKQLGH Q15346 2299 2299 R1
SRDIASDYKYKEAYRKQLGH Q15346 3271 3271 R5
SRDIASDYKYKEAYEKQKGH Q15346 3757 3757 R7
SGELASSVKYKEEYEKMKGR O35884 534 534 -
SREIASDYKYKLDHEKQKGH Q15346 3999 3999 R8
CNDVQSELKYKAEHVKQKGH Q15346 4241 4241 R9

Motif 2 width=20
Element Seqn Id St Int Rpt
DDPKMMWSIHVAKIQSDREY Q15346 3542 8 R6
DDPKMMWSMHVAKIQSDREY Q15346 2813 8 R3
DDPKMMWSMHVAKIQSDREY Q15346 3056 8 R4
DDPKMMWSMHVAKIQSDREY Q15346 2570 8 R2
DDPKLVLSMNVAKMQSEREY Q15346 2327 8 R1
DDPKIMWSLHIAKVQSDREY Q15346 3299 8 R5
DDPKIMCAIHAEKIQSEREY Q15346 3785 8 R7
TDSKLLHSLQVAKMSSEVEY O35884 558 4 -
DDNKIRWALIADKLQNEREY Q15346 4027 8 R8
DDPKLVWFEHAGQIQNERLY Q15346 4269 8 R9

Motif 3 width=20
Element Seqn Id St Int Rpt
KKEFEKWKTKFSSPVDMLGV Q15346 3562 0 R6
KKDFEKWKTKFSSPVDMLGV Q15346 2833 0 R3
KKDFEKWKTKFSSPVDMLGV Q15346 3076 0 R4
KKDFEKWKTKFSSPVDMLGV Q15346 2590 0 R2
KKDFEKWKTKFSSPVDMLGV Q15346 2347 0 R1
KKDFEKYKTRYSSPVDMLGI Q15346 3319 0 R5
KKEFQKWKTKFSSPVDMLSI Q15346 3805 0 R7
KKGFEESKTHFNLPMDMVNL O35884 578 0 -
RLDWAKWKAKIQSPVDMLSI Q15346 4047 0 R8
KEDYHKTKAKINIPADMVSV Q15346 4289 0 R9

Motif 4 width=16
Element Seqn Id St Int Rpt
LAKKCQILVSDIDYKH Q15346 3583 1 R6
LAKKCQTLVSDVDYKN Q15346 2854 1 R3
LAKKCQTLVSDVDYKN Q15346 3097 1 R4
LAYKCQTLVSDVDYKN Q15346 2611 1 R2
LAKKCQELVSDVDYKN Q15346 2368 1 R1
LAKKCQTLVSDVDYKH Q15346 3340 1 R5
LAKKCQTLVTDIYYRN Q15346 3826 1 R7
HAKKAQALASDLDYRK O35884 599 1 -
HSKNSQALVSDMDYRN Q15346 4068 1 R8
AAKQGQTLVSDIDYRN Q15346 4310 1 R9

Motif 5 width=19
Element Seqn Id St Int Rpt
HEWTCLPDQNDVIQARKAY Q15346 3601 2 R6
HQWTCLPDQSDVIHARQAY Q15346 2872 2 R3
HEWTCLPDQNDVIHARQAY Q15346 3115 2 R4
HQWTCLPDQSDVIHARQAY Q15346 2629 2 R2
HQWTCLPDQNDVVQAKKVY Q15346 2386 2 R1
HECICLPDQNDIIHARKAY Q15346 3358 2 R5
HEWTCMPDQNDIIQAKKAY Q15346 3844 2 R7
HCFTALPTDMKAEWAKKAY O35884 860 245 -
HQWTCMPDQNDVIQAKKAY Q15346 4086 2 R8
HQWMCHPDQNDVIQARKAY Q15346 4328 2 R9

Motif 6 width=17
Element Seqn Id St Int Rpt
WLRGIGWVPIGSVEVEK Q15346 3633 13 R6
WMRGIGWVSIGSLDVEK Q15346 2904 13 R3
WLRGIGWVPIGSMDVVK Q15346 3147 13 R4
WLKGIGWMTSGSLEDEK Q15346 2661 13 R2
WLRGIGWSPLGSLEAEK Q15346 2418 13 R1
WMKGIGWVPIDSLEVVR Q15346 3390 13 R5
WLRGIGWMPQGSPEVLR Q15346 3876 13 R7
WMKGTGWVATGSLHVEQ O35884 892 13 -
WLRGIGWMPNDSVSVNH Q15346 4118 13 R8
WLRGIGWIPLDSVDHVR Q15346 4360 13 R9