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PR00498

Identifier
P47PHOX  [View Relations]  [View Alignment]  
Accession
PR00498
No. of Motifs
6
Creation Date
10-MAR-1996  (UPDATE 07-JUN-1999)
Title
Neutrophil cytosol factor 1 signature
Database References

INTERPRO; IPR001655
Literature References
1. SEGAL, A.W.
The NADPH oxidase and chronic granulomatous disease.
MOL.MED.TODAY 2(3) 129-135 (1996).

Documentation
Phagocytes form the first line of defence against invasion by micro-
organisms. Engulfing of bacteria by neutrophils is accompanied by the
consumption of large amounts of oxygen - a so-called respiratory burst.
Defects in phagocytosis involving the lack of a respiratory burst give
rise to chronic granulomatous disease (CGD), in which patients are pre-
disposed to infection, often from otherwise non-pathogenic bacteria [1].
 
Regulation of the respiratory burst takes place at the phagocytic vacuole.
The process is mediated by NADPH oxidase, which transports electrons across
the plasma membrane to form superoxide (an oxygen molecule with an extra
electron that is toxic to normal cells) in the vacuole interior. The
electrons are carried across the membrane by a short electron transport
chain in the form of an unusual flavocytochrome b [1].
 
To conserve NADPH and avoid the toxic effects of superoxide, the oxidase 
remains inactive until it receives an appropriate stimulus. Activation
involves the participation of a number of cytosolic proteins, some of 
which attach to the flavocytochrome. P47phox, p67phox and p40phox are
specialised components of phagocytic cells, all of which contain SH3
domains, by means of which they attach to proline-rich regions of other
proteins. Upon activation, p47phox and p67phox are phosphorylated and,
with p40phox, translocate to the region of the plasma membrane forming
the phagocytic vacuole, where they associates with hydrophilic regions
of the flavocytochrome [1].
 
P47PHOX is a 6-element fingerprint that provides a signature for neutrophil
cytosol factor 1 (p47phox). The fingerprint was derived from an initial
alignment of 2 sequences: the motifs were drawn from short conserved
regions spanning the full alignment length. A single iteration on OWL27.0
was required to reach convergence, no further sequences being identified
beyond the starting set. A single partial match was also found, A54067,
a fragment lacking the portion of sequence bearing the last 4 motifs.
 
An update on SPTR37_9f identified a true set of 5 sequences.
Summary Information
5 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6555555
5000000
4000000
3000000
2000000
123456
True Positives
NCF1_HUMAN    NCF1_MOUSE    O43842        O70144        
O77774
Sequence Titles
NCF1_HUMAN  NEUTROPHIL CYTOSOL FACTOR 2 (NCF-1) (NEUTROPHIL NADPH OXIDASE FACTOR 1) (P47-PHOX) (NCF-47K) (47 KD AUTOSOMAL CHRONIC GRANULOMATOUS DISEASE PROTEIN) - HOMO SAPIENS (HUMAN). 
NCF1_MOUSE NEUTROPHIL CYTOSOL FACTOR 2 (NCF-1) (NEUTROPHIL NADPH OXIDASE FACTOR 1) (P47-PHOX) (NCF-47K) - MUS MUSCULUS (MOUSE).
O43842 P47-PHOX - HOMO SAPIENS (HUMAN).
O70144 P47PHOX - MUS MUSCULUS (MOUSE).
O77774 NADPH OXIDASE P47-PHOX - BOS TAURUS (BOVINE).
Scan History
OWL27_0    1  100  NSINGLE    
SPTR37_9f 2 6 NSINGLE
Initial Motifs
Motif 1  width=9
Element Seqn Id St Int Rpt
RHIALLGFE NCF1_HUMAN 7 7 -
RHIALLGFE NCF1_MOUSE 7 7 -

Motif 2 width=10
Element Seqn Id St Int Rpt
RFTEIYEFHK NCF1_HUMAN 43 27 -
KFTEIYEFHK NCF1_MOUSE 43 27 -

Motif 3 width=20
Element Seqn Id St Int Rpt
KISRCPHLLDFFKVRPDDLK NCF1_HUMAN 107 54 -
KISRCPHLLDFFKVRPDDLK NCF1_MOUSE 107 54 -

Motif 4 width=22
Element Seqn Id St Int Rpt
KKPETYLMPKDGKSTATDITGP NCF1_HUMAN 134 7 -
KKPETYLVPKDGKNNVADITGP NCF1_MOUSE 134 7 -

Motif 5 width=20
Element Seqn Id St Int Rpt
EKSESGWWFCQMKAKRGWIP NCF1_HUMAN 187 31 -
EKSESGWWFCQMKTKRGWVP NCF1_MOUSE 187 31 -

Motif 6 width=20
Element Seqn Id St Int Rpt
PPRPSADLILNRCSESTKRK NCF1_HUMAN 366 159 -
PPRPSSDLILHRCTESTKRK NCF1_MOUSE 366 159 -
Final Motifs
Motif 1  width=9
Element Seqn Id St Int Rpt
RHIALLGFE NCF1_HUMAN 7 7 -
RHIALLGFE O43842 7 7 -
RHIALLGFE O77774 7 7 -
RHIALLGFE NCF1_MOUSE 7 7 -
RHIALLGFE O70144 7 7 -

Motif 2 width=10
Element Seqn Id St Int Rpt
RFTEIYEFHK NCF1_HUMAN 43 27 -
RFTEIYEFHK O43842 43 27 -
RFTEIYEFHK O77774 43 27 -
KFTEIYEFHK NCF1_MOUSE 43 27 -
KFTEIYEFHK O70144 43 27 -

Motif 3 width=20
Element Seqn Id St Int Rpt
KISRCPHLLDFFKVRPDDLK NCF1_HUMAN 107 54 -
KISRCPHLLDFFKVRPDDLK O43842 107 54 -
KISRCPHLLNFFKVRPDDLK O77774 107 54 -
KISRCPHLLDFFKVRPDDLK NCF1_MOUSE 107 54 -
KISRCPHLLDFFKVRPDDLK O70144 107 54 -

Motif 4 width=22
Element Seqn Id St Int Rpt
KKPETYLMPKDGKSTATDITGP NCF1_HUMAN 134 7 -
KKPETYLMPKDGKSTATDITGP O43842 134 7 -
KKPETYLMPKDGKNNAADITGP O77774 134 7 -
KKPETYLVPKDGKNNVADITGP NCF1_MOUSE 134 7 -
KKPETYLVPKDGKNNVADITGP O70144 134 7 -

Motif 5 width=20
Element Seqn Id St Int Rpt
EKSESGWWFCQMKAKRGWIP NCF1_HUMAN 187 31 -
EKSESGWWFCQMKAKRGWIP O43842 187 31 -
EKNESGWWFCQMKTKRGWVP O77774 187 31 -
EKSESGWWFCQMKTKRGWVP NCF1_MOUSE 187 31 -
EKSESGWWFCQMKTKRGWVP O70144 187 31 -

Motif 6 width=20
Element Seqn Id St Int Rpt
PPRPSADLILNRCSESTKRK NCF1_HUMAN 366 159 -
PPRPSADLILNRCSESTKRK O43842 366 159 -
PPRPSADLILHRCSESTKRK O77774 368 161 -
PPRPSSDLILHRCTESTKRK NCF1_MOUSE 366 159 -
PPRPSSDLILHRCTESTKRK O70144 366 159 -