SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR00496

Identifier
NAPIN  [View Relations]  [View Alignment]  
Accession
PR00496
No. of Motifs
5
Creation Date
03-MAR-1996  (UPDATE 07-JUN-1999)
Title
Napin signature
Database References

INTERPRO; IPR000617
Literature References
1. ERICSON, M.L., RODIN, J., LENMAN, M., GLIMELIUS, K., JOSEFSSON, L.G.
AND RASK, L.
Structure of the rapeseed 1.7S storage protein, napin, and its precursor.
J.BIOL.CHEM. 261 14576-14581 (1986).
 
2. CROUCH, M.L., TENBARGE, K.M., SIMON, A.E. AND FERL, R.
cDNA clones for Brassica napus seed storage proteins: evidence from
nucleotide sequence analysis that both subunits of napin are cleaved
from a precursor polypeptide.
J.MOL.APPL.GENET. 2 273-283 (1983).

Documentation
Napins are low-molecular weight, basic storage proteins synthesised in
rape-seed embryos during seed maturation [1,2]. Sequence comparisons have
revealed that napin belongs to a diverse protein family, which includes
major allergens, trypsin inhibitors and natural anti-fungal proteins.
 
Napin comprises 2 polypeptide chains (MW 9000 and 4000) held together by
disulphide bonds. The protein is initially synthesised as a precursor of
178 residues, which is proteolytically cleaved to generate mature napin
chains, with 86 and 29 residues respectively.
 
NAPIN is a 5-element fingerprint that provides a signature for the napin
family of seed storage proteins. The fingerprint was derived from an
initial alignment of 8 sequences: the motifs were drawn from short
conserved regions spanning the full alignment length. Two iterations on
OWL27.0 were required to reach convergence, at which point a true set
comprising 33 sequences was identified. Five partial matches were also
found: these include the related mabinlins, which fail to match motifs 3
and/or 4; and a grain-softness protein, which matches motifs 2 and 4. 
 
An update on SPTR37_9f identified a true set of 20 sequences, and 5
partial matches.
Summary Information
  20 codes involving  5 elements
4 codes involving 4 elements
1 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
52020202020
444044
311001
200000
12345
True Positives
2SS1_ARATH    2SS2_ARATH    2SS2_BRANA    2SS3_ARATH    
2SS3_BRANA 2SS4_ARATH 2SSB_BRANA 2SSE_BRANA
2SSI_BRANA ALL1_BRAJU ALL1_SINAL ITRY_SINAR
Q39344 Q42413 Q42444 Q42469
Q42473 Q42490 Q42491 Q96339
True Positive Partials
Codes involving 4 elements
2SS2_CAPMA 2SS3_CAPMA 2SS4_CAPMA O04774
Codes involving 3 elements
2SS1_CAPMA
Sequence Titles
2SS1_ARATH  2S SEED STORAGE PROTEIN 1 PRECURSOR (2S ALBUMIN STORAGE PROTEIN) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS). 
2SS2_ARATH 2S SEED STORAGE PROTEIN 2 PRECURSOR (2S ALBUMIN STORAGE PROTEIN) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
2SS2_BRANA NAPIN 2 PRECURSOR (1.7S SEED STORAGE PROTEIN) - BRASSICA NAPUS (RAPE).
2SS3_ARATH 2S SEED STORAGE PROTEIN 3 PRECURSOR (2S ALBUMIN STORAGE PROTEIN) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
2SS3_BRANA NAPIN PRECURSOR (1.7S SEED STORAGE PROTEIN) - BRASSICA NAPUS (RAPE).
2SS4_ARATH 2S SEED STORAGE PROTEIN 4 PRECURSOR (2S ALBUMIN STORAGE PROTEIN) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
2SSB_BRANA NAPIN B PRECURSOR (1.7S SEED STORAGE PROTEIN) - BRASSICA NAPUS (RAPE).
2SSE_BRANA NAPIN EMBRYO SPECIFIC PRECURSOR (1.7S SEED STORAGE PROTEIN) - BRASSICA NAPUS (RAPE).
2SSI_BRANA NAPIN IA AND IB SMALL CHAIN AND LARGE CHAINS - BRASSICA NAPUS (RAPE).
ALL1_BRAJU ALLERGEN BRA J 1-E, SMALL AND LARGE CHAINS (BRA J I) - BRASSICA JUNCEA (LEAF MUSTARD) (INDIAN MUSTARD).
ALL1_SINAL ALLERGEN SIN A 1, SMALL AND LARGE CHAINS (SIN A I) - SINAPIS ALBA (WHITE MUSTARD) (BRASSICA HIRTA).
ITRY_SINAR TRYPSIN INHIBITOR (TISA) - SINAPIS ARVENSIS (CHARLOCK).
Q39344 NAPB NAPIN - BRASSICA NAPUS (RAPE).
Q42413 2S STORAGE PROTEIN - BRASSICA JUNCEA (LEAF MUSTARD) (INDIAN MUSTARD).
Q42444 NAPIN - BRASSICA CAMPESTRIS (FIELD MUSTARD).
Q42469 NAPIN - BRASSICA NAPUS (RAPE).
Q42473 2S STORAGE PROTEIN - BRASSICA CAMPESTRIS (FIELD MUSTARD).
Q42490 2S STORAGE PROTEIN - BRASSICA OLERACEA (CAULIFLOWER).
Q42491 2S STORAGE PROTEIN - BRASSICA NIGRA (BLACK MUSTARD).
Q96339 2S STORAGE PREPROPEPTIDE PRECURSOR - BRASSICA CARINATA.

2SS2_CAPMA MABINLIN II, A AND B CHAINS (SWEET PROTEIN) - CAPPARIS MASAIKAI (MABINLANG).
2SS3_CAPMA MABINLIN III, A AND B CHAINS (SWEET PROTEIN) - CAPPARIS MASAIKAI (MABINLANG).
2SS4_CAPMA MABINLIN IV, A AND B CHAINS (SWEET PROTEIN) - CAPPARIS MASAIKAI (MABINLANG).
O04774 MABINLIN PRECURSOR - CAPPARIS MASAIKAI (MABINLANG).

2SS1_CAPMA MABINLIN I-1, A AND B CHAINS (SWEET PROTEIN) - CAPPARIS MASAIKAI (MABINLANG).
Scan History
OWL27_0    2  70   NSINGLE    
SPTR37_9f 2 54 NSINGLE
Initial Motifs
Motif 1  width=22
Element Seqn Id St Int Rpt
KCRKEFQQAQHLRACQQWLHKQ 2SSE_BRANA 46 46 -
KCRKEFQQAQHLKACQQWLHKQ 2SSB_BRANA 46 46 -
KCRKEFQQAQHLKACQQWLHKQ 2SS3_BRANA 46 46 -
KCRKEFQQAQHLRACQQWLHKQ 2SS2_BRANA 46 46 -
KCRKEFQQAQHLKACQQWLHKQ BNANAPINA 46 46 -
KCRKEFQQAQHLKACQQWLHKQ 2SS1_BRANA 2 2 -
KCRKEFQQAQHLRACQQWLHKQ BNASSPB 46 46 -
KCQREFQQEQHLRACQQWIRQQ 2SSI_BRANA 4 4 -

Motif 2 width=18
Element Seqn Id St Int Rpt
QCCNELHQEEPLCVCPTL 2SS2_BRANA 104 36 -
QCCNELHQEEPLCVCPTL BNASSPB 104 36 -
QYCNELQQEEPLCVCPTL 2SS1_BRANA 59 35 -
QCCNELHQEEPLCVCPTL BNANAPINA 105 37 -
QCCNELHQEEPLCVCPTL 2SS3_BRANA 104 36 -
QCCNELHQEEPLCVCPTL 2SSB_BRANA 104 36 -
QCCNELHQEEPLCVCPTL 2SSE_BRANA 107 39 -
QCCNELYQEDQVCVCPTL 2SSI_BRANA 44 18 -

Motif 3 width=14
Element Seqn Id St Int Rpt
LKGASKAVKQQVRQ 2SS3_BRANA 121 -1 -
LKGASKAVKQQIQQ 2SS2_BRANA 121 -1 -
LKGASKAVKQQIRQ BNANAPINA 122 -1 -
LRGASKAVKQQIQQ 2SS1_BRANA 76 -1 -
LKGASKAVKQQIQQ BNASSPB 121 -1 -
LKQAAKSVRVQGQH 2SSI_BRANA 61 -1 -
LKGASKAVRQQVRQ 2SSE_BRANA 124 -1 -
LKGASKAVKQQIQQ 2SSB_BRANA 121 -1 -

Motif 4 width=15
Element Seqn Id St Int Rpt
ISRIYQTATHLPRAC BNANAPINA 150 14 -
VSRIYQTATHLPKVC BNASSPB 145 10 -
VSRIYQTATHLPKVC 2SS2_BRANA 145 10 -
ISRIYQTATHLPKVC 2SS3_BRANA 147 12 -
VSRIYQTATHLPKVC 2SSB_BRANA 145 10 -
ISRVYQTATHLPRVC 2SSE_BRANA 152 14 -
STRIYQIAKNLPNVC 2SSI_BRANA 79 4 -
VNRIYQTATHLPKVC 2SS1_BRANA 100 10 -

Motif 5 width=13
Element Seqn Id St Int Rpt
CNIPQVSVCPFQK BNASSPB 159 -1 -
CNIPQVSVCPFQK 2SS1_BRANA 114 -1 -
CNMKQIGTCPFIA 2SSI_BRANA 93 -1 -
CNIRQVSICPFQK 2SSE_BRANA 166 -1 -
CKIPQVSVCPFQK 2SSB_BRANA 159 -1 -
CNIPQVSVCPFQK 2SS3_BRANA 161 -1 -
CNIRQVSICPFQK BNANAPINA 164 -1 -
CNIPQVSVCPFQK 2SS2_BRANA 159 -1 -
Final Motifs
Motif 1  width=22
Element Seqn Id St Int Rpt
KCRKEFQQAQHLRACQQWLHKQ 2SS2_BRANA 46 46 -
KCRKEFQQAQHLRACQQWLHKQ Q42469 46 46 -
KCRKEFQQAQHLKACQQWLHKQ 2SS3_BRANA 46 46 -
KCRKEFQQAQHLRACQQWLHKQ Q42490 46 46 -
KCRKEFQQAQHLRACQQELHKQ Q96339 44 44 -
KCRKEFQQAQHLRVCQQWLHKQ Q42413 46 46 -
KCRKEFQQAQHLRACQQWLHKQ ALL1_SINAL 9 9 -
KCRKEFQQAQHLKACQQWLHKQ 2SSB_BRANA 46 46 -
KCRKEFQQAQHLRACQQWLHKQ Q39344 46 46 -
RCRKEFQQAQHLRACQQWLHKQ ALL1_BRAJU 8 8 -
KCRKEFQQAQHLKACQQWLHKQ Q42444 46 46 -
KCRKEFQQAQHLKACQQWLHKQ Q42473 46 46 -
RCRKEFQQAQHLRACQQWLHKQ ITRY_SINAR 9 9 -
KCRKEFQQAQHLRACQQWLHKQ 2SSE_BRANA 46 46 -
RCRKEFRQAQHLRACQQWLHRQ Q42491 46 46 -
KCRKEFQKEQHLRACQQLMLQQ 2SS1_ARATH 45 45 -
KCQKEFQQDQHLRACQRWMRKQ 2SS4_ARATH 44 44 -
RCQKEFQQSQHLRACQRWMSKQ 2SS3_ARATH 44 44 -
KCQKEFQQSQHLRACQKLMRMQ 2SS2_ARATH 44 44 -
KCQREFQQEQHLRACQQWIRQQ 2SSI_BRANA 4 4 -

Motif 2 width=18
Element Seqn Id St Int Rpt
QCCNELHQEEPLCVCPTL 2SS2_BRANA 104 36 -
QCCNELHQEEPLCVCPTL Q42469 104 36 -
QCCNELHQEEPLCVCPTL 2SS3_BRANA 104 36 -
QCCNELDQEEPLCVCPTL Q42490 104 36 -
QCCNELHQEEPLCVCPTL Q96339 102 36 -
QCCNELHQEEPLCVCPTL Q42413 104 36 -
QCCNELHQEEPLCVCPTL ALL1_SINAL 52 21 -
QCCNELHQEEPLCVCPTL 2SSB_BRANA 104 36 -
QCCNELHQEEPLCVCPTL Q39344 104 36 -
QCCNELHQEEPLCVCPTL ALL1_BRAJU 51 21 -
QCCNELHQEEPLCVCPTL Q42444 105 37 -
QCCNELHQEEPLCVCPTL Q42473 104 36 -
QCCNELHQEEPLCVCPTL ITRY_SINAR 52 21 -
QCCNELHQEEPLCVCPTL 2SSE_BRANA 107 39 -
QCCNELHQEEALCVCPTL Q42491 104 36 -
QCCNELRQEEPDCVCPTL 2SS1_ARATH 96 29 -
KCCSELRQEEPVCVCPTL 2SS4_ARATH 97 31 -
QCCNELRQEEPVCVCPTL 2SS3_ARATH 94 28 -
QCCSELRQEEPVCVCPTL 2SS2_ARATH 101 35 -
QCCNELYQEDQVCVCPTL 2SSI_BRANA 44 18 -

Motif 3 width=14
Element Seqn Id St Int Rpt
LKGASKAVKQQIQQ 2SS2_BRANA 121 -1 -
LKGASKAVKQQVRQ Q42469 121 -1 -
LKGASKAVKQQVRQ 2SS3_BRANA 121 -1 -
LKGASKAVKQQIQQ Q42490 121 -1 -
LKGASKAVKQQVRQ Q96339 119 -1 -
LKGASKAVKQQIQQ Q42413 121 -1 -
LKGASKAVKQQVRQ ALL1_SINAL 69 -1 -
LKGASKAVKQQIQQ 2SSB_BRANA 121 -1 -
LKGASKAVKQQIQQ Q39344 121 -1 -
LKGASKAVKQQIRQ ALL1_BRAJU 68 -1 -
LKGASKAVKQQIRQ Q42444 122 -1 -
LKGASKAVKQQVRQ Q42473 121 -1 -
LKGAAKAVKQQIQQ ITRY_SINAR 69 -1 -
LKGASKAVRQQVRQ 2SSE_BRANA 124 -1 -
LKGASKAVRQQVRQ Q42491 121 -1 -
LKQAAKAVRLQGQH 2SS1_ARATH 113 -1 -
LRQAAKAVRFQGQQ 2SS4_ARATH 114 -1 -
LKQAARAVSLQGQH 2SS3_ARATH 111 -1 -
LRQAARAVSLQGQH 2SS2_ARATH 118 -1 -
LKQAAKSVRVQGQH 2SSI_BRANA 61 -1 -

Motif 4 width=15
Element Seqn Id St Int Rpt
VSRIYQTATHLPKVC 2SS2_BRANA 145 10 -
ISRIYQTATHLPKVC Q42469 147 12 -
ISRIYQTATHLPKVC 2SS3_BRANA 147 12 -
VSRIYQTATHLPKVC Q42490 145 10 -
ISRIYQTATHLPKVC Q96339 145 12 -
VSRIYQTATHLPKVC Q42413 145 10 -
ISRIYQTATHLPKVC ALL1_SINAL 95 12 -
VSRIYQTATHLPKVC 2SSB_BRANA 145 10 -
VSRIYQTRTNLPKVC Q39344 145 10 -
ISRIYQTATHLPRVC ALL1_BRAJU 97 15 -
ISRIYQTATHLPRAC Q42444 150 14 -
ISRIYQTSTHLPRVC Q42473 145 10 -
IRRIYQTATHLPKVC ITRY_SINAR 98 15 -
ISRVYQTATHLPRVC 2SSE_BRANA 152 14 -
ISRIYQTATHLPRVC Q42491 145 10 -
VRKIYQTAKHLPNVC 2SS1_ARATH 131 4 -
VRKIYQAAKYLPNIC 2SS4_ARATH 133 5 -
SRKIYQSAKYLPNIC 2SS3_ARATH 129 4 -
SRKIYKTAKYLPNIC 2SS2_ARATH 136 4 -
STRIYQIAKNLPNVC 2SSI_BRANA 79 4 -

Motif 5 width=13
Element Seqn Id St Int Rpt
CNIPQVSVCPFQK 2SS2_BRANA 159 -1 -
CNIPQVSVCPFQK Q42469 161 -1 -
CNIPQVSVCPFQK 2SS3_BRANA 161 -1 -
CNIPQVSVCPFQK Q42490 159 -1 -
CNIPQVSVCPFQK Q96339 159 -1 -
CNIPQVSVCPFQK Q42413 159 -1 -
CNIPQVSVCPFKK ALL1_SINAL 109 -1 -
CKIPQVSVCPFQK 2SSB_BRANA 159 -1 -
CNIPQVSVCPFQK Q39344 159 -1 -
CNIPRVSICPFQK ALL1_BRAJU 111 -1 -
CNIRQVSICPFQK Q42444 164 -1 -
CNIRQVSICPFQK Q42473 159 -1 -
CNIPQVQVCPFNK ITRY_SINAR 112 -1 -
CNIRQVSICPFQK 2SSE_BRANA 166 -1 -
CNIPQVSVCPFQK Q42491 159 -1 -
CDIPQVDVCPFNI 2SS1_ARATH 145 -1 -
CKIQQVGVCPFQI 2SS4_ARATH 147 -1 -
CKIQQVGECPFQT 2SS3_ARATH 143 -1 -
CKIQQVGECPFQT 2SS2_ARATH 150 -1 -
CNMKQIGTCPFIA 2SSI_BRANA 93 -1 -