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PR00484

Identifier
PBPGOBP  [View Relations]  [View Alignment]  
Accession
PR00484
No. of Motifs
6
Creation Date
02-APR-1996  (UPDATE 07-JUN-1999)
Title
Pheromone/general odorant binding protein signature
Database References

INTERPRO; IPR000746
Literature References
1. MCKENNA, M.P., HEKMAT-SCAFE, D.S., GAINES, P. AND CARLSON, J.R.
Putative Drosophila pheromone-binding proteins expressed in a subregion
of the olfactory system.
J.BIOL.CHEM. 269(23) 16340-16347 (1994).

Documentation
Pheromone binding proteins (PBPs) are abundant, secreted antennal proteins 
present in olfactory sensilla - specifically, they lie in the lymph that
surrounds the dendrites of olfactory receptor neurons [1]. They are thought
to transport hydrophobic pheromones across this aqueous fluid to receptor
molecules in the dendritic membranes. Another group of proteins, the
so-called general odorant-binding proteins (GOBPs) have also been found
in moth antennae. They share a high level of sequence similarity with the
PBPs and are believed to carry odorants rather than pheromones. This
family is distinct from the vertebrate odorant-binding proteins (OBPs).
 
PBPGOBP is a 6-element fingerprint that provides a signature for the
PBP/GOBP family. The fingerprint was derived from an initial alignment of
7 sequences: the motifs were drawn from short conserved regions spanning
virtually the full alignment length. Two iterations on OWL27.1 were
required to reach convergence, at which point a true set comprising 14
sequences was identified.
 
An update on SPTR37_9f identified a true set of 17 sequences, and 1
partial match.
Summary Information
  17 codes involving  6 elements
1 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6171717171717
5011111
4000000
3000000
2000000
123456
True Positives
O16105        O16106        O77227        OBP1_ANTPE    
OBP1_BOMMO OBP1_HELVI OBP1_MANSE OBP2_ANTPE
OBP2_BOMMO OBP2_HELVI OBP2_MANSE PBP1_ANTPE
PBP2_ANTPE PBP_ANTPO PBP_BOMMO PBP_HELVI
PBP_MANSE
True Positive Partials
Codes involving 5 elements
O61561
Sequence Titles
O16105      PHEROMONE BINDING PROTEIN 1 PRECURSOR - LYMANTRIA DISPAR (GYPSY MOTH). 
O16106 PHEROMONE BINDING PROTEIN 2 - LYMANTRIA DISPAR (GYPSY MOTH).
O77227 PHEROMONE BINDING PROTEIN - HELIOTHIS ZEA (CORN EARWORM) (BOLLWORM).
OBP1_ANTPE GENERAL ODORANT-BINDING PROTEIN 1 PRECURSOR (GOBP 1) - ANTHERAEA PERNYI (CHINESE OAK SILK MOTH).
OBP1_BOMMO GENERAL ODORANT-BINDING PROTEIN 1 PRECURSOR (GOBP1) (GOBP2) - BOMBYX MORI (SILK MOTH).
OBP1_HELVI GENERAL ODORANT-BINDING PROTEIN 1 PRECURSOR (GOBP 1) - HELIOTHIS VIRESCENS (NOCTUID MOTH) (OWLET MOTH).
OBP1_MANSE GENERAL ODORANT-BINDING PROTEIN 1 PRECURSOR (GOBP 1) - MANDUCA SEXTA (TOBACCO HAWKMOTH) (TOBACCO HORNWORM).
OBP2_ANTPE GENERAL ODORANT-BINDING PROTEIN 2 PRECURSOR (GOBP 2) (PHEROMONE- BINDING PROTEIN 10) (APR-10) - ANTHERAEA PERNYI (CHINESE OAK SILK MOTH).
OBP2_BOMMO GENERAL ODORANT-BINDING PROTEIN 2 PRECURSOR (GOBP2) (GOBP1) - BOMBYX MORI (SILK MOTH).
OBP2_HELVI GENERAL ODORANT-BINDING PROTEIN 2 PRECURSOR (GOBP 2) - HELIOTHIS VIRESCENS (NOCTUID MOTH) (OWLET MOTH).
OBP2_MANSE GENERAL ODORANT-BINDING PROTEIN 2 PRECURSOR (GOBP 2) - MANDUCA SEXTA (TOBACCO HAWKMOTH) (TOBACCO HORNWORM).
PBP1_ANTPE PHEROMONE-BINDING PROTEIN 1 PRECURSOR (PBP 1) (APR-1) - ANTHERAEA PERNYI (CHINESE OAK SILK MOTH).
PBP2_ANTPE PHEROMONE-BINDING PROTEIN 2 PRECURSOR (PBP 2) (APR-2) - ANTHERAEA PERNYI (CHINESE OAK SILK MOTH).
PBP_ANTPO PHEROMONE-BINDING PROTEIN PRECURSOR (PBP) - ANTHERAEA POLYPHEMUS (POLYPHEMUS MOTH).
PBP_BOMMO PHEROMONE-BINDING PROTEIN PRECURSOR (PBP) - BOMBYX MORI (SILK MOTH).
PBP_HELVI PHEROMONE-BINDING PROTEIN PRECURSOR (PBP) - HELIOTHIS VIRESCENS (NOCTUID MOTH) (OWLET MOTH).
PBP_MANSE PHEROMONE-BINDING PROTEIN PRECURSOR (PBP) - MANDUCA SEXTA (TOBACCO HAWKMOTH) (TOBACCO HORNWORM).

O61561 PHEROMONE BINDING PROTEIN 2 PRECURSOR - MAMESTRA BRASSICAE.
Scan History
OWL27_1    2  100  NSINGLE    
SPTR37_9f 2 23 NSINGLE
Initial Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
EVMSHVTAHFGKALEECREESGL OBP2_MANSE 23 23 -
EVMKNLCLNFGKALDECKAEMNL MOTPBPB 29 29 -
EIIKNLSQNFCKAMDQCKQELNI S14269 24 24 -
EVMKNLCMNYGKAMDQCKQELNL S14448 25 25 -
EVMSHVTAHFGKALEECRDESGL A60292 22 22 -
QVMKDVTLGFGQALEQCREESQL OBP1_MANSE 25 25 -
EIMKNLSNNFGKAMDQCKDELSL PBP_ANTPO 24 24 -

Motif 2 width=17
Element Seqn Id St Int Rpt
VADLYNFWKDDYVMTDR PBP_ANTPO 51 4 -
MEEFFHFWREDFKFEHR OBP1_MANSE 52 4 -
LNEFKHFWSEDFDVVHR A60292 49 4 -
IADLYNFWKDDYVMTDR S14448 52 4 -
IADLYNFWKDDYVMTDR S14269 51 4 -
KDDFANFWVEGYEVSNR MOTPBPB 56 4 -
MDEFKHFWREDFEVVHR OBP2_MANSE 50 4 -

Motif 3 width=16
Element Seqn Id St Int Rpt
LAGCAINCMATKLDVV S14269 68 0 -
LAGCAINCLSTKLDIV S14448 69 0 -
ELGCAIICMSNKFSLL A60292 66 0 -
ELGCALQCMSRHFNLL OBP1_MANSE 69 0 -
LAGCAINCLATKLDVV PBP_ANTPO 68 0 -
ELGCAIICMSNKFELL OBP2_MANSE 67 0 -
DTGCAILCLSKKLDMI MOTPBPB 73 0 -

Motif 4 width=15
Element Seqn Id St Int Rpt
LSAKMVNLIHNCEKQ A60292 105 23 -
MAQQLVDIIHGCEKS S14269 107 23 -
LSKTMVELIHNCELQ OBP1_MANSE 108 23 -
MAQQLVDIIHGCEKS PBP_ANTPO 107 23 -
LSEKMVQLIHNCEKQ OBP2_MANSE 106 23 -
MAKQLLDIVHNCENS MOTPBPB 112 23 -
MAHELVDIIHGCEKS S14448 108 23 -

Motif 5 width=19
Element Seqn Id St Int Rpt
DDCDRVVKVAACFKKDAKK OBP2_MANSE 126 5 -
DACLKTLDIAKCFKKEIHK MOTPBPB 132 5 -
DECDRVVKVAACFKVDAKK A60292 125 5 -
DKCIKTMDIAMCFKKEIHK S14448 128 5 -
DKCMKTIDVAMCFKKEIHK S14269 127 5 -
DKCMKTIDVAMCFKKEIHK PBP_ANTPO 127 5 -
DHCWRILRVAECFKISCTK OBP1_MANSE 128 5 -

Motif 6 width=10
Element Seqn Id St Int Rpt
EVAMIEAVIE A60292 149 5 -
DLVVGEVLAE MOTPBPB 158 7 -
EVAMIEAVIE OBP2_MANSE 150 5 -
DLVIGEVLAE PBP_ANTPO 153 7 -
EVMMAEFIME OBP1_MANSE 154 7 -
DVVLGEVLAE S14269 153 7 -
DLVVGEVLAE S14448 154 7 -
Final Motifs
Motif 1  width=23
Element Seqn Id St Int Rpt
EVMKNLSLNFGKALDECKKEMTL PBP_BOMMO 25 25 -
DVIKNLSMNFAKPLEDCKKEMDL O77227 23 23 -
DVMKNLSMNFAKPLEDCKKEMDL PBP_HELVI 23 23 -
EVMSHVTAHFGKALEECREESGL OBP2_HELVI 24 24 -
EVMSHVTAHFGKALEECRDESGL OBP2_ANTPE 22 22 -
EIMKNLSNNFGKAMDQCKDELSL PBP_ANTPO 24 24 -
EVMKNLCMNYGKAMDQCKQELNL PBP2_ANTPE 25 25 -
EVMSHVTAHFGKTLEECREESGL OBP1_BOMMO 22 22 -
DVMKNLCLNFGKALDECKAEMNL PBP_MANSE 29 29 -
NVMKDVTLGFGQALEKCREESQL OBP1_ANTPE 25 25 -
QVMKDVTLGFGQALEQCREESQL OBP1_MANSE 25 25 -
EIIKNLSQNFCKAMDQCKQELNI PBP1_ANTPE 24 24 -
EVMSHVTAHFGKALEECREESGL OBP2_MANSE 23 23 -
YVMKDVTLGFGQALEQCREESQL OBP2_BOMMO 21 21 -
NVMKDVTLGFGQALDKCREESQL OBP1_HELVI 22 22 -
EVMKQMTINFAKPMEACKQELNV O16105 22 22 -
DVMHQMALKFGKPIKLCQQELGA O16106 23 23 -

Motif 2 width=17
Element Seqn Id St Int Rpt
MDEFKHFWREDFEVVHR OBP2_MANSE 50 4 -
NEDFYNFWKEGYEIKNR PBP_BOMMO 52 4 -
TTDFYNFWKEGYEFTNR O77227 50 4 -
TTDFYNFWKEGYEFTNR PBP_HELVI 50 4 -
LEEFQHFWREDFEVVHR OBP2_HELVI 51 4 -
LNEFKHFWSEDFDVVHR OBP2_ANTPE 49 4 -
VADLYNFWKDDYVMTDR PBP_ANTPO 51 4 -
IADLYNFWKDDYVMTDR PBP2_ANTPE 52 4 -
LDEFKHFWSDDFDVVHR OBP1_BOMMO 49 4 -
KDDFANFWVEGYEVSNR PBP_MANSE 56 4 -
MEEFFHFWSEDFKFEHR OBP1_ANTPE 52 4 -
MEEFFHFWREDFKFEHR OBP1_MANSE 52 4 -
IADLYNFWKDDYVMTDR PBP1_ANTPE 51 4 -
MEEFFHFWNDDFKFEHR OBP2_BOMMO 48 4 -
MEEFFHFWRDDFKFEHR OBP1_HELVI 49 4 -
MQDFFNFWKEGYQITNR O16105 49 4 -
VKEFLDFWKDGYVMKDR O16106 50 4 -

Motif 3 width=16
Element Seqn Id St Int Rpt
ELGCAIICMSNKFELL OBP2_MANSE 67 0 -
ETGCAIMCLSTKLNML PBP_BOMMO 69 0 -
QTGCAILCLSSKLELL O77227 67 0 -
HTGCASLCLSSKLELL PBP_HELVI 67 0 -
ELGCAIICMSNKFSLL OBP2_HELVI 68 0 -
ELGCAIICMSNKFSLL OBP2_ANTPE 66 0 -
LAGCAINCLATKLDVV PBP_ANTPO 68 0 -
LAGCAINCLSTKLDIV PBP2_ANTPE 69 0 -
ELGCAIICMSNKFSLM OBP1_BOMMO 66 0 -
DTGCAILCLSKKLDMI PBP_MANSE 73 0 -
ELGCAILCMSRHFNLL OBP1_ANTPE 69 0 -
ELGCALQCMSRHFNLL OBP1_MANSE 69 0 -
LAGCAINCMATKLDVV PBP1_ANTPE 68 0 -
ELGCAIQCMSRHFNLL OBP2_BOMMO 65 0 -
ELGCAIQCMSRHFNLL OBP1_HELVI 66 0 -
EAGCVILCLAKKLELL O16105 66 0 -
QTGCMLICMAMKLELL O16106 67 0 -

Motif 4 width=15
Element Seqn Id St Int Rpt
MAQQLVDIIHGCEKS PBP1_ANTPE 107 23 -
LSEKMVQLIHNCEKQ OBP2_MANSE 106 23 -
MAQQLIDIVHGCEKS PBP_BOMMO 108 23 -
MAKQLVDLIHGCAQS O77227 106 23 -
MAKQLVDMIHGCSQS PBP_HELVI 106 23 -
LSEKLVELIHNCEKK OBP2_HELVI 107 23 -
LSAKMVNLIHNCEKQ OBP2_ANTPE 105 23 -
MAQQLVDIIHGCEKS PBP_ANTPO 107 23 -
MAHELVDIIHGCEKS PBP2_ANTPE 108 23 -
LAEKMVKLIHNCEKQ OBP1_BOMMO 105 23 -
MAKQLLDIIHNCENS PBP_MANSE 112 23 -
LSKHMVNLIHSCEQQ OBP1_ANTPE 108 23 -
LSKTMVELIHNCELQ OBP1_MANSE 108 23 -
LSQKMIDMIHTCEKT OBP2_BOMMO 104 23 -
LARQMVELIHSCEKQ OBP1_HELVI 105 23 -
MAKQLLDIKHSCEKV O16105 105 23 -
MAQQIIDIVHGCTTT O16106 106 23 -

Motif 5 width=19
Element Seqn Id St Int Rpt
DDCDRVVKVAACFKKDAKK OBP2_MANSE 126 5 -
DKCIWTLGVATCFKAEIHK PBP_BOMMO 128 5 -
DPCMKTLNVAKCFKAKIHE O77227 127 6 -
DPCMKALNVAKCFKAKIHE PBP_HELVI 127 6 -
DDCDRVVKVAACFKVDAKA OBP2_HELVI 127 5 -
DECDRVVKVAACFKVDAKK OBP2_ANTPE 125 5 -
DKCMKTIDVAMCFKKEIHK PBP_ANTPO 127 5 -
DKCIKTMDIAMCFKKEIHK PBP2_ANTPE 128 5 -
DDCTRVVKVAACFKKDSRK OBP1_BOMMO 125 5 -
DACLKTLDIAKCFKKEIHK PBP_MANSE 132 5 -
DHCWRILRVAECFKRSCQE OBP1_ANTPE 128 5 -
DHCWRILRVAECFKISCTK OBP1_MANSE 128 5 -
DKCMKTIDVAMCFKKEIHK PBP1_ANTPE 127 5 -
DHCWRILRVAECFKDACNK OBP2_BOMMO 124 5 -
DHCWRISHLADCFKSSCVQ OBP1_HELVI 125 5 -
DPCQTMLNLAMCFKAEIHK O16105 126 6 -
DPCQRAVNVAMCFKAHVHK O16106 128 7 -

Motif 6 width=10
Element Seqn Id St Int Rpt
ELLVADFLAE O16106 154 7 -
EVAMIEAVIE OBP2_MANSE 150 5 -
DVAVGEILAE PBP_BOMMO 154 7 -
DLVVGEVLAE O77227 153 7 -
ELVVGEVLAE PBP_HELVI 153 7 -
EVTMIEAVME OBP2_HELVI 151 5 -
EVAMIEAVIE OBP2_ANTPE 149 5 -
DLVIGEVLAE PBP_ANTPO 153 7 -
DLVVGEVLAE PBP2_ANTPE 154 7 -
EVAMIEAVIE OBP1_BOMMO 149 5 -
DLVVGEVLAE PBP_MANSE 158 7 -
ELLMAEFIME OBP1_ANTPE 154 7 -
EVMMAEFIME OBP1_MANSE 154 7 -
DVVLGEVLAE PBP1_ANTPE 153 7 -
ELILAEFIME OBP2_BOMMO 150 7 -
ELMMTEFIME OBP1_HELVI 151 7 -
DVAVGELLAD O16105 152 7 -