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PR00483

Identifier
BACPHPHTASE  [View Relations]  [View Alignment]  
Accession
PR00483
No. of Motifs
5
Creation Date
18-FEB-1996  (UPDATE 06-JUN-1999)
Title
Bacterial acid phosphatase class A signature
Database References

PROSITE; PS01157 ACID_PHOSPH_CL_A
BLOCKS; BL01157
PFAM; PF01178 Bac_acid_phos_I
INTERPRO; IPR001011
Literature References
1. THALLER, M.C., BERLUTTI, F., SCHIPPA, S. ,LOMBARDI, G.
AND ROSSOLINI, G.M.
Characterization and sequence of phoC, the principal phosphate-
irrepressible acid phosphatase of Morganella morganii.
MICROBIOLOGY 140 1341-1350 (1994).
 
2. KASAHARA, M., NAKATA, A. AND SHINAGAWA, H.
Molecular analysis of the Salmonella typhimurium phoN gene, which encodes 
nonspecific acid phosphatase.
J.BACTERIOL. 173 6760-6765 (1991).

Documentation
Class A bacterial acid phosphatases are a group of related proteins that
catalyse the conversion of an orthophosphoric monoester and water to an
alcohol and orthophosphate [1,2]. This reaction is non-specific and occurs
in the periplasm [1,2]. The enzyme is found as a homotetramer in Proteus
morganii [1] and possibly as a homodimer in Salmonella typhimurium, where 
it is regulated by a 2-component regulatory system formed by the products
of the phoP and phoQ genes [2].
 
BACPHPHTASE is a 5-element fingerprint that provides a signature for class A
bacterial acid phosphatases. The fingerprint was derived from an initial
alignment of 4 sequences: the motifs were drawn from conserved regions
spanning virtually the full alignment length, motif 3 including the central
area encoded by PROSITE pattern ACID_PHOSPH_CL_A (PS01157). Two iterations
on OWL27.0 were required to reach convergence, at which point a true set
comprising 5 sequences was identified.
 
An update on SPTR37_9f identified a true set of 7 sequences.
Summary Information
7 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
577777
400000
300000
200000
12345
True Positives
O50542        O87188        PHOC_MORMO    PHON_PROST    
PHON_SALTY PPA_ZYMMO Q59909
Sequence Titles
O50542      NONSPECIFIC PHOSPHATASE PRECURSOR - SHIGELLA FLEXNERI. 
O87188 ACID PHOSPHATASE (PIACP) PRECURSOR (PIACP) - PREVOTELLA INTERMEDIA.
PHOC_MORMO MAJOR PHOSPHATE-IRREPRESSIBLE ACID PHOSPHATASE PRECURSOR (EC 3.1.3.2) (HPAP) - MORGANELLA MORGANII (PROTEUS MORGANII).
PHON_PROST NONSPECIFIC ACID PHOSPHATASE PRECURSOR (EC 3.1.3.2) (NSAP) - PROVIDENCIA STUARTII.
PHON_SALTY NONSPECIFIC ACID PHOSPHATASE PRECURSOR (EC 3.1.3.2) (NSAP) - SALMONELLA TYPHIMURIUM.
PPA_ZYMMO ACID PHOSPHATASE PRECURSOR (EC 3.1.3.2) - ZYMOMONAS MOBILIS.
Q59909 ATP DIPHOSPHOHYDROLASE, APYRASE PRECURSOR (EC 3.6.1.5) (ATP-DIPHOSPHATASE) (ADENOSINE DIPHOSPHATASE) (ADPASE) - SHIGELLA FLEXNERI.
Scan History
OWL27_0    2  100  NSINGLE    
SPTR37_9f 2 7 NSINGLE
Initial Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
YEKGRLLRNTERGEQAAKDA PHON_PROST 67 67 -
YEKGRMLRNTERGKQAQADA PHOC_MORMO 67 67 -
YFKGYAIKGSPRWKQAAEDA PHON_SALTY 57 57 -
FNSTRQLKGSTRWALATQDA PPA_ZYMMO 67 67 -

Motif 2 width=19
Element Seqn Id St Int Rpt
AKEKYMRIRPFAFYGVATC PHON_PROST 132 45 -
AKEHYMRIRPFAFYGTETC PHOC_MORMO 132 45 -
AKKYYMRTRPFVLFNHSTC PHON_SALTY 122 45 -
AKNNWNRKRPFVDTDQPIC PPA_ZYMMO 131 44 -

Motif 3 width=27
Element Seqn Id St Int Rpt
LSKNGSYPSGHTAIGWASALVLSEINP PHON_PROST 158 7 -
LSTNGSYPSGHTSIGWATALVLAEVNP PHOC_MORMO 158 7 -
LRKNGSYPSGHTAYGTLLALVLSEARP PHON_SALTY 148 7 -
LGKQGSYPSGHTTIGWSVALILAELIP PPA_ZYMMO 157 7 -

Motif 4 width=25
Element Seqn Id St Int Rpt
ILKRGYELGQSRVICGYHWQSDVDA PHON_PROST 190 5 -
ILERGYQLGQSRVICGYHWQSDVDA PHOC_MORMO 190 5 -
LARRGWEFGQSRVICGAHWQSDVDA PHON_SALTY 180 5 -
ILQRGQIFGTSRIVCGAHWFSDVQA PPA_ZYMMO 189 5 -

Motif 5 width=19
Element Seqn Id St Int Rpt
ATLHSNPEFQKQLQKAKDE PHON_PROST 224 9 -
ATLHSDPAFQAQLAKAKQE PHOC_MORMO 224 9 -
ARLQTIPAFQKSLAKVREE PHON_SALTY 214 9 -
AALHGDADFRRDMELARKE PPA_ZYMMO 223 9 -
Final Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
YEKGRLLRNTERGKLAAEDA O50542 67 67 -
YEKGRLLRNTERGEQAAKDA PHON_PROST 67 67 -
YEKGRMLRNTERGKQAQADA PHOC_MORMO 67 67 -
YQWGKMQRNTPRGDQAVADA O87188 69 69 -
YEFGRSLRDANRVRLASEDA Q59909 60 60 -
YFKGYAIKGSPRWKQAAEDA PHON_SALTY 57 57 -
FNSTRQLKGSTRWALATQDA PPA_ZYMMO 67 67 -

Motif 2 width=19
Element Seqn Id St Int Rpt
AKEYYMRIRPFAFYGVSTC O50542 132 45 -
AKEKYMRIRPFAFYGVATC PHON_PROST 132 45 -
AKEHYMRIRPFAFYGTETC PHOC_MORMO 132 45 -
AKDHYMRVRPFAFYNEMTC O87188 134 45 -
AKEYYKRVRPFVIYKDATC Q59909 123 43 -
AKKYYMRTRPFVLFNHSTC PHON_SALTY 122 45 -
AKNNWNRKRPFVDTDQPIC PPA_ZYMMO 131 44 -

Motif 3 width=27
Element Seqn Id St Int Rpt
LSRNGSYPSGHTSIGWATALVLSEINP O50542 158 7 -
LSKNGSYPSGHTAIGWASALVLSEINP PHON_PROST 158 7 -
LSTNGSYPSGHTSIGWATALVLAEVNP PHOC_MORMO 158 7 -
LSTNGSYPSGHTAIGWATALVLSEINI O87188 160 7 -
MAITGSYPSGHASFGWAVALILAEINP Q59909 149 7 -
LRKNGSYPSGHTAYGTLLALVLSEARP PHON_SALTY 148 7 -
LGKQGSYPSGHTTIGWSVALILAELIP PPA_ZYMMO 157 7 -

Motif 4 width=25
Element Seqn Id St Int Rpt
ILKRGYELGDSRVICGYHWQSDVDA O50542 190 5 -
ILKRGYELGQSRVICGYHWQSDVDA PHON_PROST 190 5 -
ILERGYQLGQSRVICGYHWQSDVDA PHOC_MORMO 190 5 -
ILERGYQMGQSRVICGYHWQSDVDA O87188 192 5 -
ILRRGYEFGESRVICGAHWQSDVEA Q59909 181 5 -
LARRGWEFGQSRVICGAHWQSDVDA PHON_SALTY 180 5 -
ILQRGQIFGTSRIVCGAHWFSDVQA PPA_ZYMMO 189 5 -

Motif 5 width=19
Element Seqn Id St Int Rpt
ATLHSNPVFQAQLQKAKDE O50542 224 9 -
ATLHSNPEFQKQLQKAKDE PHON_PROST 224 9 -
ATLHSDPAFQAQLAKAKQE PHOC_MORMO 224 9 -
ARLHADPAFQAQLEKAKKE O87188 226 9 -
AVLHNTPEFTKSLSEAKKE Q59909 215 9 -
ARLQTIPAFQKSLAKVREE PHON_SALTY 214 9 -
AALHGDADFRRDMELARKE PPA_ZYMMO 223 9 -