| Literature References | 1. BANKS, R.D., BLAKE, C.C., EVANS, P.R., HASER, R., RICE, D.W.,
HARDY, G.W., MERRETT, M. AND PHILLIPS, A.W.
Sequence, structure and activity of phosphoglycerate kinase: a possible
hinge-bending enzyme.
NATURE 179 773-777 (1979).
2. WATSON, H.C., WALKER, N.P.C., SHAW, P.J., BRYANT, T.N., WENDELL, P.L.,
FOTHERGILL, L.A., PERKINS, R.E., CONROY, S.C., DOBSON, M.J., TUITE, M.F.,
KINGSMAN, A.J. AND KINGSMAN, S.M.
Sequence and structure of yeast phosphoglycerate kinase.
EMBO J. 1 1635-1640 (1982).
3. FUJII, H., KRIETSCH, W.K. AND YOSHIDA, A.
A single amino acid substitution (Asp leads to Asn) in a phosphoglycerate
kinase variant (PGK Munchen) associated with enzyme deficiency.
J.BIOL.CHEM. 255 6421-6423 (1980).
|
| Documentation | Phosphoglycerate kinase (PGK) catalyses the second step of glycolysis, the
reversible conversion of 1,3-diphospho-D-glycerate and ADP to 3-phospho-D-
glycerate and ATP [1-3]. This is an important reaction in the generation
of ATP required for the glycolytic pathways of aerobes and anaerobes, and
for carbon fixation in plants [1].
The enzyme exists as a monomer split into 2 almost equally-sized domains,
which essentially correspond to the N- and C-halves of the protein (except
for the last 15 residues, which loop back into the N-terminal domain [1,2]).
This extended 2-domain structure is probably associated with large-scale
`hinge-bending' conformational changes, similar to those found in
hexokinase [1,2].
At the core of each domain is a 6-stranded parallel beta-sheet surrounded by
alpha-helices [1,2]. The structures of the domains are highly similar, with
only a slight change in the order of the strands, yet they share no sequence
similarity [1,2]. As with other glycolytic enzymes, the secondary structure
elements tend to adopt repeating beta-alpha units, PGK containing 12 such
units, 6 in each domain [1,2].
Mutations in the PGK gene may result in chronic diseases (such as hemolytic
anaemia), which can be accompanied by either mental retardation or muscular
disease (rhabdomyolysis) [3].
PHGLYCKINASE is a 9-element fingerprint that provides a signature for the
phosphoglycerate kinase family. The fingerprint was derived from an initial
alignment of 14 sequences: motifs 1-4 lie in the N-terminal domain; part
of motif 5 corresponds to the interdomain hinge; and motifs 6-9 lie in the
C-terminal domain, motif 7 spanning conserved residues in the substrate-
binding cleft encoded by PROSITE pattern PGLYCERATE_KINASE (PS0111). Two
iterations on OWL27.0 were required to reach convergence, at which point a
true set comprising 70 sequences was identified. Seven partial matches were
also found, all of which are PGK fragments.
An update on SPTR37_9f identified a true set of 91 sequences.
|
| Sequence Titles | O74233 PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - GLOMUS MOSSEAE.
O81394 PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) PRECURSOR - SOLANUM TUBEROSUM (POTATO).
O82158 CYTOSOLIC PHOSPHOGLYCERATE KINASE 1 - POPULUS NIGRA (LOMBARDY POPLAR).
O82159 PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - POPULUS NIGRA (LOMBARDY POPLAR).
O82160 PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - POPULUS NIGRA (LOMBARDY POPLAR).
O84699 PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - CHLAMYDIA TRACHOMATIS.
PGK1_HUMAN PHOSPHOGLYCERATE KINASE 1 (EC 2.7.2.3) (PRIMER RECOGNITION PROTEIN 2) (PRP 2) - HOMO SAPIENS (HUMAN).
PGK1_MACEU PHOSPHOGLYCERATE KINASE 1 (EC 2.7.2.3) - MACROPUS EUGENII (TAMMAR WALLABY).
PGK1_MOUSE PHOSPHOGLYCERATE KINASE 1 (EC 2.7.2.3) - MUS MUSCULUS (MOUSE).
PGK1_RHINI PHOSPHOGLYCERATE KINASE 1 (EC 2.7.2.3) - RHIZOPUS NIVEUS.
PGK1_TRYCO PHOSPHOGLYCERATE KINASE, CYTOSOLIC (EC 2.7.2.3) - TRYPANOSOMA CONGOLENSE.
PGK2_HUMAN PHOSPHOGLYCERATE KINASE, TESTIS SPECIFIC (EC 2.7.2.3) - HOMO SAPIENS (HUMAN).
PGK2_MOUSE PHOSPHOGLYCERATE KINASE, TESTIS SPECIFIC (EC 2.7.2.3) - MUS MUSCULUS (MOUSE).
PGK2_RAT PHOSPHOGLYCERATE KINASE, TESTIS SPECIFIC (EC 2.7.2.3) - RATTUS NORVEGICUS (RAT).
PGK2_RHINI PHOSPHOGLYCERATE KINASE 2 (EC 2.7.2.3) - RHIZOPUS NIVEUS.
PGKA_CRIFA PHOSPHOGLYCERATE KINASE, GLYCOSOMAL (A) (EC 2.7.2.3) - CRITHIDIA FASCICULATA.
PGKA_TRYBB PHOSPHOGLYCERATE KINASE, A (EC 2.7.2.3) (ALLELE 2) - TRYPANOSOMA BRUCEI BRUCEI.
PGKB_CRIFA PHOSPHOGLYCERATE KINASE, CYTOSOLIC (B) (EC 2.7.2.3) - CRITHIDIA FASCICULATA.
PGKB_LEIMA PHOSPHOGLYCERATE KINASE, CYTOSOLIC (B) (EC 2.7.2.3) - LEISHMANIA MAJOR.
PGKB_LEIME PHOSPHOGLYCERATE KINASE, CYTOSOLIC (B) (EC 2.7.2.3) - LEISHMANIA MEXICANA.
PGKB_TRYBB PHOSPHOGLYCERATE KINASE, CYTOSOLIC (B) (EC 2.7.2.3) (ALLELE 2) - TRYPANOSOMA BRUCEI BRUCEI.
PGKC_ALCEU PHOSPHOGLYCERATE KINASE, CHROMOSOMAL (EC 2.7.2.3) - ALCALIGENES EUTROPHUS.
PGKC_CRIFA PHOSPHOGLYCERATE KINASE, GLYCOSOMAL (C) (EC 2.7.2.3) - CRITHIDIA FASCICULATA.
PGKC_LEIMA PHOSPHOGLYCERATE KINASE, GLYCOSOMAL (C) (EC 2.7.2.3) - LEISHMANIA MAJOR.
PGKC_LEIME PHOSPHOGLYCERATE KINASE, GLYCOSOMAL (C) (EC 2.7.2.3) - LEISHMANIA MEXICANA.
PGKC_TRYBB PHOSPHOGLYCERATE KINASE, GLYCOSOMAL (C) (EC 2.7.2.3) - TRYPANOSOMA BRUCEI BRUCEI.
PGKD_TRYBB PHOSPHOGLYCERATE KINASE, A (EC 2.7.2.3) (ALLELE 4) - TRYPANOSOMA BRUCEI BRUCEI.
PGKE_TRYBB PHOSPHOGLYCERATE KINASE, CYTOSOLIC (B) (EC 2.7.2.3) (ALLELE 4) - TRYPANOSOMA BRUCEI BRUCEI.
PGKG_TRYCO PHOSPHOGLYCERATE KINASE, GLYCOSOMAL (EC 2.7.2.3) - TRYPANOSOMA CONGOLENSE.
PGKH_CHLRE PHOSPHOGLYCERATE KINASE, CHLOROPLAST PRECURSOR (EC 2.7.2.3) - CHLAMYDOMONAS REINHARDTII.
PGKH_TOBAC PHOSPHOGLYCERATE KINASE, CHLOROPLAST PRECURSOR (EC 2.7.2.3) - NICOTIANA TABACUM (COMMON TOBACCO).
PGKH_WHEAT PHOSPHOGLYCERATE KINASE, CHLOROPLAST PRECURSOR (EC 2.7.2.3) - TRITICUM AESTIVUM (WHEAT).
PGKP_ALCEU PHOSPHOGLYCERATE KINASE, PLASMID (EC 2.7.2.3) - ALCALIGENES EUTROPHUS.
PGKY_TOBAC PHOSPHOGLYCERATE KINASE, CYTOSOLIC (EC 2.7.2.3) - NICOTIANA TABACUM (COMMON TOBACCO).
PGKY_WHEAT PHOSPHOGLYCERATE KINASE, CYTOSOLIC (EC 2.7.2.3) - TRITICUM AESTIVUM (WHEAT).
PGK_APLCA PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - APLYSIA CALIFORNICA (CALIFORNIA SEA HARE).
PGK_AQUAE PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - AQUIFEX AEOLICUS.
PGK_ARCFU PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - ARCHAEOGLOBUS FULGIDUS.
PGK_ASPOR PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - ASPERGILLUS ORYZAE.
PGK_BACME PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - BACILLUS MEGATERIUM.
PGK_BACST PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - BACILLUS STEAROTHERMOPHILUS.
PGK_BACSU PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - BACILLUS SUBTILIS.
PGK_BORBU PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - BORRELIA BURGDORFERI (LYME DISEASE SPIROCHETE).
PGK_CAEEL PROBABLE PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - CAENORHABDITIS ELEGANS.
PGK_CANAL PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - CANDIDA ALBICANS (YEAST).
PGK_CANMA PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - CANDIDA MALTOSA (YEAST).
PGK_CHICK PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - GALLUS GALLUS (CHICKEN).
PGK_CHLTR PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - CHLAMYDIA TRACHOMATIS.
PGK_CLOAB PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - CLOSTRIDIUM ACETOBUTYLICUM.
PGK_CORGL PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - CORYNEBACTERIUM GLUTAMICUM (BREVIBACTERIUM FLAVUM).
PGK_CRIGR PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - CRICETULUS GRISEUS (CHINESE HAMSTER).
PGK_DROME PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - DROSOPHILA MELANOGASTER (FRUIT FLY).
PGK_ECOLI PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - ESCHERICHIA COLI.
PGK_EMENI PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - EMERICELLA NIDULANS (ASPERGILLUS NIDULANS).
PGK_EUPCR PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - EUPLOTES CRASSUS.
PGK_HAEIN PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - HAEMOPHILUS INFLUENZAE.
PGK_HALVA PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - HALOARCULA VALLISMORTIS.
PGK_HELPY PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - HELICOBACTER PYLORI (CAMPYLOBACTER PYLORI).
PGK_HORSE PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - EQUUS CABALLUS (HORSE).
PGK_KLULA PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - KLUYVEROMYCES LACTIS (YEAST).
PGK_LACDE PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - LACTOBACILLUS DELBRUECKII (SUBSP. BULGARICUS).
PGK_METJA PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - METHANOCOCCUS JANNASCHII.
PGK_METTH PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - METHANOBACTERIUM THERMOAUTOTROPHICUM.
PGK_MYCGE PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - MYCOPLASMA GENITALIUM.
PGK_MYCLE PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - MYCOBACTERIUM LEPRAE.
PGK_MYCPN PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - MYCOPLASMA PNEUMONIAE.
PGK_MYCTU PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - MYCOBACTERIUM TUBERCULOSIS.
PGK_NEUCR PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - NEUROSPORA CRASSA.
PGK_OXYNO PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - OXYTRICHA NOVA.
PGK_PENCH PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - PENICILLIUM CHRYSOGENUM.
PGK_PENCI PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - PENICILLIUM CITRINUM.
PGK_PLAFA PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - PLASMODIUM FALCIPARUM.
PGK_PYRHO PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - PYROCOCCUS HORIKOSHII.
PGK_PYRWO PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - PYROCOCCUS WOESEI.
PGK_SCHMA PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - SCHISTOSOMA MANSONI (BLOOD FLUKE).
PGK_SCHPO PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - SCHIZOSACCHAROMYCES POMBE (FISSION YEAST).
PGK_SULSO PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - SULFOLOBUS SOLFATARICUS.
PGK_SYNY3 PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
PGK_TETTH PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - TETRAHYMENA THERMOPHILA.
PGK_THEMA PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - THERMOTOGA MARITIMA.
PGK_THETH PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - THERMUS AQUATICUS (SUBSP. THERMOPHILUS).
PGK_TREPA PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - TREPONEMA PALLIDUM.
PGK_TRIRE PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - TRICHODERMA REESEI (HYPOCREA JECORINA).
PGK_TRIVI PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - TRICHODERMA VIRIDE.
PGK_XANFL PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - XANTHOBACTER FLAVUS.
PGK_YARLI PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - YARROWIA LIPOLYTICA (CANDIDA LIPOLYTICA).
PGK_YEAST PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
PGK_ZYMMO PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - ZYMOMONAS MOBILIS.
Q37743 PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - TRYPANOSOMA BRUCEI BRUCEI.
Q37744 PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - TRYPANOSOMA BRUCEI BRUCEI.
Q37745 PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) - TRYPANOSOMA BRUCEI BRUCEI.
|