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PR00472

Identifier
CASNKINASEII  [View Relations]  [View Alignment]  
Accession
PR00472
No. of Motifs
6
Creation Date
22-FEB-1996  (UPDATE 06-JUN-1999)
Title
Casein kinase II regulatory subunit family signature
Database References

PROSITE; PS01101 CK2_BETA
BLOCKS; BL01101
PFAM; PF01214 CK_II_beta
INTERPRO; IPR000704
Literature References
1. JAKOBI, R., VOSS, H. AND PYERIN, W.
Human phosvitin/casein kinase type II. Molecular cloning and sequencing of
full-length cDNA encoding subunit beta.
EUR.J.BIOCHEMISTRY 183 227-233 (1989).
 
2. VOSS, A., WIRKNER, U., JAKOBI, R., HEWITT, N., SCHWAGER, C., 
ZIMMERMANN., J., ANSORGE, W. AND PYERIN, W.
Structure of the gene encoding human casein kinase II subunit beta.
J.BIOL.CHEM. 266 13706-13711 (1991).
 
3. BIDWAI, A.P., REED, J.C. AND GLOVER, C.V.C.
Cloning and disruption of CKB1, the gene encoding the 38kDa beta subunit
of Saccharomyces cerevisiae caesin kinase II (CKII). Deletion of CKII
regulatory subunits elicits a salt-sensitive phenotype.
J.BIOL.CHEM. 270 10395-10404 (1995).

Documentation
Casein kinase, a ubiquitous, well-conserved protein kinase involved in
cell metabolism and differentiation [1,2], is characterised by its 
preference for Ser or Thr in acidic stretches of amino acids [1,2]. The
enzyme is a tetramer of 2 alpha- and 2 beta-subunits [1,2]. However, some
species (e.g., mammals) possess 2 related forms of the alpha-subunit (alpha
and alpha'), while others (e.g., fungi) possess 2 related beta-subunits
(beta and beta') [3]. 
 
The alpha-subunit is the catalytic unit and contains regions characteristic
of serine/threonine protein kinases [1,2]. The beta-subunit is believed to
be regulatory, possessing an N-terminal auto-phosphorylation site, an
internal acidic domain, and a potential metal-binding motif [3]. The
mammalian beta-subunit gene promoter shares common features with those of
other mammalian protein kinases and is closely related to the promoter of
the regulatory subunit of cAMP-dependent protein kinase [3].
 
CASNKINASEII is a 6-element fingerprint that provides a signature for
the casein kinase II regulatory subunit. The fingerprint was derived from 
an initial alignment of 7 sequences: the motifs were drawn from conserved
regions spanning virtually the full alignment length, motifs 4 and 5
including the region encoded by PROSITE pattern CK2_BETA (PS01101), a
Cys-rich region that may be involved in binding a metal ion such as zinc.
Two iterations on OWL27.0 were required to reach convergence, at which point
a true set comprising 15 sequences was identified. A single partial match
(DROSUSTEB) was also found, a Drosophila stellate supressor protein fragment
lacking the portion of sequence bearing the last 3 motifs.
 
An update on SPTR37_9f identified a true set of 16 sequences.
Summary Information
16 codes involving  6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
6161616161616
5000000
4000000
3000000
2000000
123456
True Positives
KC2B_ARATH    KC2B_BRARE    KC2B_CAEEL    KC2B_CANAL    
KC2B_DROME KC2B_HUMAN KC2B_SCHPO KC2B_SPOFR
KC2B_XENLA KC2B_YEAST KC2C_ARATH KC2C_YEAST
KC2D_ARATH KC2E_ARATH SSL_DROME STEL_DROME
Sequence Titles
KC2B_ARATH  CASEIN KINASE II BETA CHAIN (CK II) (EC 2.7.1.37) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS). 
KC2B_BRARE CASEIN KINASE II, BETA CHAIN (CK II) (EC 2.7.1.37) - BRACHYDANIO RERIO (ZEBRAFISH) (ZEBRA DANIO).
KC2B_CAEEL CASEIN KINASE II, BETA CHAIN (CK II) (EC 2.7.1.37) - CAENORHABDITIS ELEGANS.
KC2B_CANAL CASEIN KINASE II BETA CHAIN (CK II) (EC 2.7.1.37) - CANDIDA ALBICANS (YEAST).
KC2B_DROME CASEIN KINASE II, BETA CHAIN (CK II) (EC 2.7.1.37) - DROSOPHILA MELANOGASTER (FRUIT FLY).
KC2B_HUMAN CASEIN KINASE II, BETA CHAIN (CK II) (EC 2.7.1.37) (PHOSVITIN) - HOMO SAPIENS (HUMAN), MUS MUSCULUS (MOUSE), RATTUS NORVEGICUS (RAT), BOS TAURUS (BOVINE), SUS SCROFA (PIG), ORYCTOLAGUS CUNICULUS (RABBIT), AND GALLUS GALLUS (CHICKEN).
KC2B_SCHPO CASEIN KINASE II BETA CHAIN (CK II) (EC 2.7.1.37) - SCHIZOSACCHAROMYCES POMBE (FISSION YEAST).
KC2B_SPOFR CASEIN KINASE II, BETA CHAIN (CK II) (EC 2.7.1.37) - SPODOPTERA FRUGIPERDA (FALL ARMYWORM).
KC2B_XENLA CASEIN KINASE II, BETA CHAIN (CK II) (EC 2.7.1.37) (PHOSVITIN) - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
KC2B_YEAST CASEIN KINASE II BETA CHAIN (CK II) (EC 2.7.1.37) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
KC2C_ARATH CASEIN KINASE II BETA' CHAIN (CK II) (EC 2.7.1.37) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
KC2C_YEAST CASEIN KINASE II BETA' CHAIN (CK II) (EC 2.7.1.37) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
KC2D_ARATH CASEIN KINASE II BETA-3 CHAIN (CK II) (EC 2.7.1.37) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
KC2E_ARATH PUTATIVE CASEIN KINASE II BETA-4 CHAIN (CK II) (EC 2.7.1.37) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
SSL_DROME SUSTE-LIKE PROTEIN (SU(STE)-LIKE) - DROSOPHILA MELANOGASTER (FRUIT FLY).
STEL_DROME STELLATE PROTEIN - DROSOPHILA MELANOGASTER (FRUIT FLY).
Scan History
OWL27_0    2  100  NSINGLE    
SPTR37_9f 2 17 NSINGLE
Initial Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
SWVTWFCGLRGNEFFCE KC2B_DROME 8 8 -
SWISWFCNLRGNEFFCE KC2C_ARATH 95 95 -
SWITWFCGLRGNEFFCE KC2B_CAEEL 8 8 -
YWVDWFLGLKGNEFFCE KC2B_SCHPO 15 15 -
SWIDWFLGIKGNQFLCR STEL_DROME 10 10 -
SWISWFCGLRGNEFFCE KC2B_BOVIN 4 4 -
SWISWFCGLRGNEFFCE KC2B_XENLA 8 8 -

Motif 2 width=15
Element Seqn Id St Int Rpt
VDEDYIQDKFNLTGL KC2B_DROME 25 0 -
VDEEYIQDRFNLTGL KC2B_CAEEL 25 0 -
VDEDYIQDKFNLTGL KC2B_BOVIN 21 0 -
VPTDYVQDTFNQMGL STEL_DROME 27 0 -
VDEDFIQDRFNLTGL KC2B_SCHPO 32 0 -
VDEDYIQDKFNLTGL KC2B_XENLA 25 0 -
VDDDYIQDDFNLCGL KC2C_ARATH 112 0 -

Motif 3 width=22
Element Seqn Id St Int Rpt
YGLIHARYILTNRGISQMVEKW KC2B_CAEEL 79 39 -
YGLIHARYILTNRGIAQMLEKY KC2B_BOVIN 76 40 -
YGLIHARYILTNRGIAQMLEKY KC2B_XENLA 80 40 -
YGLIHARYILTNRGIAQMIEKY KC2B_DROME 80 40 -
YGMIHARFILTSKGLASMLDKY KC2C_ARATH 167 40 -
YGLIHARYILTAQGLYKMLEKY KC2B_SCHPO 84 37 -
YGMIHARYIRSERGLIAMHRKY STEL_DROME 71 29 -

Motif 4 width=22
Element Seqn Id St Int Rpt
DFGHCPRVLCNGQPMLPVGLSD KC2B_SCHPO 109 3 -
DFGYCPRVYCENQPMLPIGLSD KC2B_BOVIN 101 3 -
DFGSCPNISCDRQNTLPVGLSA STEL_DROME 96 3 -
DFGYCPRVYCENQPMLPIGLSD KC2B_XENLA 105 3 -
DFGHCPRVYCESQPMLPLGLSD KC2B_DROME 105 3 -
DFGRCPRVYCCGQPCLPVGQSD KC2C_ARATH 192 3 -
DFGVCPRVYCENQPMLPIGLSD KC2B_CAEEL 104 3 -

Motif 5 width=22
Element Seqn Id St Int Rpt
IPGEAMVKTYCPKCIDVYTPKS KC2B_DROME 127 0 -
IPGEAMVKLYCPKCMDVYTPKS KC2B_XENLA 127 0 -
IPRASTVKIYCPKCEDVYYPRS KC2C_ARATH 214 0 -
VPGEAMVKLYCPRCNMVFVPRS KC2B_CAEEL 126 0 -
IAHAKSVKLYCPRCEDVYTPKS KC2B_SCHPO 131 0 -
VWGKSTVKIHCPRCKSNFHPKS STEL_DROME 118 0 -
IPGEAMVKLYCPKCMDVYTPKS KC2B_BOVIN 123 0 -

Motif 6 width=18
Element Seqn Id St Int Rpt
DGAYFGTTFPHLFLMTYG KC2C_ARATH 242 6 -
DGAMFGPSFPDIFFSLLP STEL_DROME 144 4 -
DGAYFGTGFPHMLFMVHP KC2B_XENLA 155 6 -
DGAYFGTSFPHMLFQVYP KC2B_SCHPO 159 6 -
DGAYFGTGFPHMLFMVHP KC2B_BOVIN 151 6 -
DGSYFGTGFPHMLFFVHP KC2B_CAEEL 154 6 -
DGAYFGTGFPHMLFMVHP KC2B_DROME 155 6 -
Final Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
SWISWFCGLRGNEFFCE KC2B_BRARE 8 8 -
SWISWFCGLRGNEFFCE KC2B_HUMAN 8 8 -
SWISWFCGLRGNEFFCE KC2B_XENLA 8 8 -
SWVTWFCGLRGNEFFCE KC2B_DROME 8 8 -
SWISWFCGLRGNEFFCE KC2B_SPOFR 8 8 -
SWISWFCNLRGNEFFCE KC2B_ARATH 100 100 -
SWISWFCNLRGNEFFCE KC2E_ARATH 95 95 -
SWISWFCNLRGNEFFCE KC2C_ARATH 95 95 -
SWITWFCGLRGNEFFCE KC2B_CAEEL 8 8 -
SWISWFCNLRGNDFFCE KC2D_ARATH 89 89 -
YWVDWFLGLKGNEFFCE KC2B_SCHPO 15 15 -
MWIDLFLGRKGHEYFCD KC2C_YEAST 37 37 -
SWIDWFLGIKGHEFSCR SSL_DROME 13 13 -
SWIDWFLGIKGNQFLCR STEL_DROME 10 10 -
PWIQQLCELFGHDYFVQ KC2B_CANAL 11 11 -
EWIPSFCSRFGHEYFCQ KC2B_YEAST 25 25 -

Motif 2 width=15
Element Seqn Id St Int Rpt
VDEDYIQDKFNLTGL KC2B_BRARE 25 0 -
VDEDYIQDKFNLTGL KC2B_HUMAN 25 0 -
VDEDYIQDKFNLTGL KC2B_XENLA 25 0 -
VDEDYIQDKFNLTGL KC2B_DROME 25 0 -
VDEDYINDKFNLTGL KC2B_SPOFR 25 0 -
VDDDYIQDDFNLCGL KC2B_ARATH 117 0 -
VDEDYIQDDFNLCGL KC2E_ARATH 112 0 -
VDDDYIQDDFNLCGL KC2C_ARATH 112 0 -
VDEEYIQDRFNLTGL KC2B_CAEEL 25 0 -
VDEDYIQDDFNLCGL KC2D_ARATH 106 0 -
VDEDFIQDRFNLTGL KC2B_SCHPO 32 0 -
VDPEYITDRFNLMNL KC2C_YEAST 54 0 -
VPNEYIQDKFNLTGL SSL_DROME 30 0 -
VPTDYVQDTFNQMGL STEL_DROME 27 0 -
VSQDFIEDDFNLTGL KC2B_CANAL 28 0 -
VPTEFIEDDFNMTSL KC2B_YEAST 42 0 -

Motif 3 width=22
Element Seqn Id St Int Rpt
YGLIHARYILTNRGIAQMLEKY KC2B_BRARE 80 40 -
YGLIHARYILTNRGIAQMLEKY KC2B_HUMAN 80 40 -
YGLIHARYILTNRGIAQMLEKY KC2B_XENLA 80 40 -
YGLIHARYILTNRGIAQMIEKY KC2B_DROME 80 40 -
YGLIHARYILTNRGIGQMLEKF KC2B_SPOFR 80 40 -
YGLIHARYILTSKGLAAMLDKY KC2B_ARATH 172 40 -
YGLIHVRYILTTKGMAAMMEKY KC2E_ARATH 167 40 -
YGMIHARFILTSKGLASMLDKY KC2C_ARATH 167 40 -
YGLIHARYILTNRGISQMVEKW KC2B_CAEEL 79 39 -
YGLIHVRYILTTKGMAAMTEKY KC2D_ARATH 161 40 -
YGLIHARYILTAQGLYKMLEKY KC2B_SCHPO 84 37 -
YGLIHARYIITIKGLQKMYAKY KC2C_YEAST 110 41 -
YGMIHARYIVSPRGIEDMRLKY SSL_DROME 73 28 -
YGMIHARYIRSERGLIAMHRKY STEL_DROME 71 29 -
YGLIHARYIVSKQGLTAMASKF KC2B_CANAL 108 65 -
YGLIHARFILTKPGLQAMAEKF KC2B_YEAST 127 70 -

Motif 4 width=22
Element Seqn Id St Int Rpt
DFGYCPRVYCENQPMLPIGLSD KC2B_BRARE 105 3 -
DFGYCPRVYCENQPMLPIGLSD KC2B_HUMAN 105 3 -
DFGYCPRVYCENQPMLPIGLSD KC2B_XENLA 105 3 -
DFGHCPRVYCESQPMLPLGLSD KC2B_DROME 105 3 -
DFGHCPRVYCECQPMLPLGLSD KC2B_SPOFR 105 3 -
DFGRCPRVYCCGQPCLPVGQSD KC2B_ARATH 197 3 -
DFGRCPRVFCCGQSCLPVGQSD KC2E_ARATH 192 3 -
DFGRCPRVYCCGQPCLPVGQSD KC2C_ARATH 192 3 -
DFGVCPRVYCENQPMLPIGLSD KC2B_CAEEL 104 3 -
DFGRCPRVFCCGQSCLPVGQSD KC2D_ARATH 186 3 -
DFGHCPRVLCNGQPMLPVGLSD KC2B_SCHPO 109 3 -
DFGRCPRVYCNLQQLLPVGLHD KC2C_YEAST 135 3 -
DFGSCPRVFCKRQKVLPVGLHD SSL_DROME 98 3 -
DFGSCPNISCDRQNTLPVGLSA STEL_DROME 96 3 -
DFGSCPRYFCDGMHLIPVGSTD KC2B_CANAL 133 3 -
EFGTCPRYYCNGMQLLPCGLSD KC2B_YEAST 152 3 -

Motif 5 width=22
Element Seqn Id St Int Rpt
IPGEAMVKLYCPKCMDVYTPKS KC2B_BRARE 127 0 -
IPGEAMVKLYCPKCMDVYTPKS KC2B_HUMAN 127 0 -
IPGEAMVKLYCPKCMDVYTPKS KC2B_XENLA 127 0 -
IPGEAMVKTYCPKCIDVYTPKS KC2B_DROME 127 0 -
VPGEAMVKLYCPRCMDVYTPKS KC2B_SPOFR 127 0 -
LPRSSTVKIYCPKCEDIYYPRS KC2B_ARATH 219 0 -
IPRSSTVKIYCPKCEDIYYPRS KC2E_ARATH 214 0 -
IPRASTVKIYCPKCEDVYYPRS KC2C_ARATH 214 0 -
VPGEAMVKLYCPRCNMVFVPRS KC2B_CAEEL 126 0 -
IPRSSTVKIYCPKCEDISYPRS KC2D_ARATH 208 0 -
IAHAKSVKLYCPRCEDVYTPKS KC2B_SCHPO 131 0 -
IPGIDCVKLYCPSCEDLYIPKS KC2C_YEAST 157 0 -
VWDKAQVKIYCPSCNNVYIPLP SSL_DROME 120 0 -
VWGKSTVKIHCPRCKSNFHPKS STEL_DROME 118 0 -
VPGQETVRLFCPCCNDIYIPSS KC2B_CANAL 155 0 -
TVGKHTVRLYCPSCQDLYLPQS KC2B_YEAST 174 0 -

Motif 6 width=18
Element Seqn Id St Int Rpt
DGAYFGTGFPHMLFMVHP KC2B_BRARE 155 6 -
DGAYFGTGFPHMLFMVHP KC2B_HUMAN 155 6 -
DGAYFGTGFPHMLFMVHP KC2B_XENLA 155 6 -
DGAYFGTGFPHMLFMVHP KC2B_DROME 155 6 -
DGAYFGTGFPHMVFMVHP KC2B_SPOFR 155 6 -
DGAYFGTTFPHLFLMTYG KC2B_ARATH 247 6 -
DGAYFGTTFPHLFLMAYG KC2E_ARATH 242 6 -
DGAYFGTTFPHLFLMTYG KC2C_ARATH 242 6 -
DGSYFGTGFPHMLFFVHP KC2B_CAEEL 154 6 -
DGAYFGTTFPHLFLMTYG KC2D_ARATH 236 6 -
DGAYFGTSFPHMLFQVYP KC2B_SCHPO 159 6 -
DGAYFGTSFPGMFLQAFP KC2C_YEAST 185 6 -
DGAMFGTSFPHMFFMQLP SSL_DROME 147 5 -
DGAMFGPSFPDIFFSLLP STEL_DROME 144 4 -
DGAFFGTTFPGLLVKMFP KC2B_CANAL 183 6 -
EGAFWGTSFPGVFLKHFK KC2B_YEAST 202 6 -