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PR00459

Identifier
ASPEROXIDASE  [View Relations]  [View Alignment]  
Accession
PR00459
No. of Motifs
11
Creation Date
03-APR-1996  (UPDATE 10-JUN-1999)
Title
Plant ascorbate peroxidase signature
Database References
PRINTS; PR00458 PEROXIDASE
INTERPRO; IPR002207
PDB; 1APX; 1CCP
SCOP; 1APX; 1CCP
CATH; 1APX; 1CCP
Literature References
1. LI, H. AND POULOS, T.L.
Structural variation in heme enzymes: a comparative analysis of
peroxidase and P450 crystal structures.
STRUCTURE 2 461-464 (1994).
 
2. WELINDER, K.G.
Superfamily of plant, fungal and bacterial peroxidases.
CURR.OPIN.STRUCT.BIOL. 2 388-393 (1992).
 
3. DALTON, D.A.
Ascorbate peroxidase.
IN PEROXIDASES IN CHEMISTRY AND BIOLOGY, EVERSE, J., EVERSE, K.E. AND
GRISHAM, M.B., EDS. VOL.II PP.139-153 (1991). CRC PRESS, BOCA RATON.
 
4. PATTERSON, W.R. AND POULOS, T.L.
Crystal structure of recombinant pea cytosolic ascorbate peroxidase.
BIOCHEMISTRY 34 4331-4341 (1995).

Documentation
Peroxidases are haem-containing enzymes that use hydrogen peroxide as
the electron acceptor to catalyse a number of oxidative reactions.
Most haem peroxidases follow the reaction scheme:
 
Fe(3+) + H2O2            --> [Fe(4+)=O]R' (Compound I) + H2O
[Fe(4+)=O]R' + substrate --> [Fe(4+)=O]R (Compound II) + oxidised substrate
[Fe(4+)=O]R  + substrate -->  Fe(3+) + H2O + oxidised substrate
 
In this mechanism, the enzyme reacts with one equivalent of H2O2 to give 
[Fe(4+)=O]R' (compound I). This is a two-electron oxidation/reduction 
reaction where H2O2 is reduced to water and the enzyme is oxidised. One 
oxidising equivalent resides on iron, giving the oxyferryl [Fe(4+)=O] 
intermediate, while in many peroxidases the porphyrin (R) is oxidised to 
the porphyrin pi-cation radical (R'). Compound I then oxidises an organic 
substrate to give a substrate radical [1].
 
Peroxidases are found in bacteria, fungi, plants and animals. On the basis
of sequence similarity, fungal, plant and bacterial peroxidases can be
viewed as members of a superfamily consisting of 3 major classes [2]. Class
I, the intracellular peroxidases, includes yeast cytochrome c peroxidase
(CCP), ascorbate peroxidase (AP) and bacterial catalase-peroxidases.
 
In chloroplasts of higher plants, oxygen consumption in the absence of
electron acceptors is accompanied by production of H2O2 and activated
forms of oxygen. Chloroplasts contain several protective systems (such as
superoxide dismutase (SOD), alpha-tocopherol and carotenoids), which are
effective against various forms of activated oxygen. However, they lack
catalase, and the disposal of H2O2 is accomplished by other means [3].
 
Ascorbic acid is a strong antioxidant that is effective in scavenging 
superoxide (O2-'), hydroxyl (OH') radicals and singlet oxygen. It can
also remove H2O2 in the following reaction:
       Ascorbate + H2O2  --> dehydroascorbate + 2 H2O
Ascorbate peroxidase (AP) is the main enzyme responsible for hydrogen
peroxide removal in the chloroplasts and cytosol of higher plants [3].
 
The 3D structure of pea cytosolic ascorbate peroxidase has an overall fold
virtually identical to that of CCP [4]. The protein consists of 2 all-alpha
domains, between which is embedded the haem group. The most pronounced
difference between the AP and CCP structures is the absence of an anti-
parallel beta-hairpin between the G and H helices in the AP molecule.
 
ASPEROXIDASE is an 11-element fingerprint that provides a signature for 
ascorbate peroxidases. The fingerprint was derived from an initial 
alignment of 8 sequences: the motifs were drawn from conserved regions 
spanning virtually the full alignment length - motif 2 encodes the B helix
and contains invariant active site residues (Arg, Phe and the "distal" His)
that form a ligand pocket for hydrogen peroxide (cf. motif 1 of signature
PEROXIDASE and PROSITE pattern PEROXIDASE_2 (PS00436)); motifs 4 and 5 
encode the C and D helices, respectively; motifs 5 and 6 correspond to
motifs 2 and 3 of PRINTS signature PEROXIDASE; motif 8 contains the
invariant "proximal" His, which serves the axial ligand of the haem iron
(cf. PROSITE pattern PEROXIDASE_1 (PS00435) and motif 4 of signature
PEROXIDASE); motif 9 corresponds to motif 5 of signature PEROXIDASE; and 
motifs 10 and 11 span the H, I and J helices. Two iterations on OWL27.1
were required to reach convergence, at which point a true set comprising 13
sequences was identified. Several partial matches were also found: the most
similar sequence is yeast CCP, which lacks motifs 1 and 3; the remaining
sequences, i.e. those matching 3 or 4 motifs and most of those matching 
just 2, belong to the fungal, plant and bacterial peroxidase superfamily.
 
An update on SPTR37_9f identified a true set of 26 sequences, and 16
partial matches.
Summary Information
  26 codes involving 11 elements
0 codes involving 10 elements
1 codes involving 9 elements
0 codes involving 8 elements
3 codes involving 7 elements
3 codes involving 6 elements
2 codes involving 5 elements
1 codes involving 4 elements
2 codes involving 3 elements
4 codes involving 2 elements
Composite Feature Index
112626262626262626262626
1000000000000
901111011111
800000000000
703030303333
602030203233
502020002022
400010010101
302002000200
201002101102
1234567891011
True Positives
APX1_ARATH    APX1_PEA      O48919        O49122        
O49159 O65161 O65634 O81603
O81604 O81810 P93404 P93657
Q39006 Q39780 Q39843 Q40589
Q41712 Q41772 Q42459 Q42564
Q42661 Q42909 Q42941 Q43758
Q43824 Q96399
True Positive Partials
Codes involving 9 elements
Q41371
Codes involving 7 elements
O46921 O46922 Q37274
Codes involving 6 elements
O49822 Q42592 Q42593
Codes involving 5 elements
O23886 O81333
Codes involving 4 elements
CCPR_YEAST
Codes involving 3 elements
CATA_BACST O59651
Codes involving 2 elements
O28050 O48677 O73955 O81772
Sequence Titles
APX1_ARATH  L-ASCORBATE PEROXIDASE, CYTOSOLIC (EC 1.11.1.11) (AP) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS). 
APX1_PEA L-ASCORBATE PEROXIDASE, CYTOSOLIC (EC 1.11.1.11) (AP) - PISUM SATIVUM (GARDEN PEA).
O48919 CYTOSOLIC ASCORBATE PEROXIDASE (EC 1.11.1.11) (L-ASCORBATE PEROXIDASE) - FRAGARIA ANANASSA (STRAWBERRY).
O49122 ASCORBATE PEROXIDASE - BRASSICA JUNCEA (LEAF MUSTARD) (INDIAN MUSTARD).
O49159 CYTOSOLIC ASCORBATE PEROXIDASE (EC 1.11.1.11) (L-ASCORBATE PEROXIDASE) - FRAGARIA ANANASSA (STRAWBERRY).
O65161 CYTOSOLIC ASCORBATE PEROXIDASE (EC 1.11.1.11) - ZANTEDESCHIA AETHIOPICA (WHITE CALLA LILY).
O65634 PUTATIVE ASCORBATE PEROXIDASE - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O81603 ASCORBATE PEROXIDASE - MESEMBRYANTHEMUM CRYSTALLINUM (COMMON ICE PLANT).
O81604 ASCORBATE PEROXIDASE - MESEMBRYANTHEMUM CRYSTALLINUM (COMMON ICE PLANT).
O81810 ASCORBATE PEROXIDASE - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
P93404 ASCORBATE PEROXIDASE (EC 1.11.1.11) - ORYZA SATIVA (RICE).
P93657 L-ASCORBATE PEROXIDASE (EC 1.11.1.11) - BRASSICA NAPUS (RAPE).
Q39006 ASCORBATE PEROXIDASE - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
Q39780 ASCORBATE PEROXIDASE - GOSSYPIUM HIRSUTUM (UPLAND COTTON).
Q39843 ASCORBATE PEROXIDASE 2 - GLYCINE MAX (SOYBEAN).
Q40589 CYTOSOLIC ASCORBATE PEROXIDASE - NICOTIANA TABACUM (COMMON TOBACCO).
Q41712 ASCORBATE PEROXIDASE - VIGNA UNGUICULATA (COWPEA).
Q41772 CYTOSOLIC ASCORBATE PEROXIDASE - ZEA MAYS (MAIZE).
Q42459 ASCORBATE PEROXIDASE - SPINACIA OLERACEA (SPINACH).
Q42564 ASCORBATE PEROXIDASE (EC 1.11.1.11) (L-ASCORBATE PEROXIDASE) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
Q42661 L-ASCORBATE PEROXIDASE (EC 1.11.1.11) - CAPSICUM ANNUUM (BELL PEPPER).
Q42909 ASCORBATE PEROXIDASE (EC 1.11.1.11) (L-ASCORBATE PEROXIDASE) - MESEMBRYANTHEMUM CRYSTALLINUM (COMMON ICE PLANT).
Q42941 ASCORBATE PEROXIDASE (EC 1.11.1.11) (L-ASCORBATE PEROXIDASE) - NICOTIANA TABACUM (COMMON TOBACCO).
Q43758 ASCORBATE PEROXIDASE (EC 1.11.1.11) (L-ASCORBATE PEROXIDASE) - GLYCINE MAX (SOYBEAN).
Q43824 L-ASCORBATE PEROXIDASE (EC 1.11.1.11) - RAPHANUS SATIVUS (RADISH).
Q96399 CYTOSOLIC ASCORBATE PEROXIDASE - CUCUMIS SATIVUS (CUCUMBER).

Q41371 ASCORBATE PEROXIDASE (EC 1.11.1.11) - SPINACIA OLERACEA (SPINACH).

O46921 THYLAKOID-BOUND ASCORBATE PEROXIDASE - SPINACIA OLERACEA (SPINACH).
O46922 STROMAL ASCORBATE PEROXIDASE - SPINACIA OLERACEA (SPINACH).
Q37274 THYLAKOID-BOUND ASCORBATE PEROXIDASE PRECURSOR (EC 1.11.1.11) (L-ASCORBATE PEROXIDASE) - SPINACIA OLERACEA (SPINACH).

O49822 ASCORBATE PEROXIDASE (EC 1.11.1.11) (L-ASCORBATE PEROXIDASE) - CHLAMYDOMONAS REINHARDTII.
Q42592 STROMAL ASCORBATE PEROXIDASE (EC 1.11.1.11) (L-ASCORBATE PEROXIDASE) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
Q42593 THYLAKOID-BOUND ASCORBATE PEROXIDASE (EC 1.11.1.11) (L-ASCORBATE PEROXIDASE) - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).

O23886 STROMAL ASCORBATE PEROXIDASE PRECURSOR - CUCURBITA MAXIMA (PUMPKIN) (WINTER SQUASH).
O81333 THYLAKOID-BOUND L-ASCORBATE PEROXIDASE PRECURSOR (EC 1.11.1.11) - MESEMBRYANTHEMUM CRYSTALLINUM (COMMON ICE PLANT).

CCPR_YEAST CYTOCHROME C PEROXIDASE PRECURSOR (EC 1.11.1.5) (CCP) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).

CATA_BACST PEROXIDASE/CATALASE (EC 1.11.1.6) - BACILLUS STEAROTHERMOPHILUS.
O59651 PEROXIDASE/CATALASE (EC 1.11.1.6) - HALOARCULA MARISMORTUI (HALOBACTERIUM MARISMORTUI).

O28050 PEROXIDASE / CATALASE (PERA) - ARCHAEOGLOBUS FULGIDUS.
O48677 PUTATIVE PEROXIDASE - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O73955 HYDROPEROXIDASE - HALOBACTERIUM SALINARIUM.
O81772 PEROXIDASE - LIKE PROTEIN - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
Scan History
OWL27_1    2  500  NSINGLE    
SPTR37_9f 2 38 NSINGLE
Initial Motifs
Motif 1  width=21
Element Seqn Id St Int Rpt
YKKAVEKCRRKLRGLIAEKNC APX1_ARATH 11 11 -
YQKAVEKAKKKLRGFIAEKRC SOYASCPER 12 12 -
YQKEIEKCKRKLRGLIAEKNC S43157 12 12 -
YQKAIEKAKRKLRGFIAEKKC A45116 12 12 -
RVIEAARRDLHRSLVQDNNNS SPIAP 13 13 -
YLKAVDKCKRKLRGLIAEKNC NTU15933 12 12 -
YMEAIEGARRDLVALVQSKNC MCU43561 6 6 -
YLKEIDKARRDLRALIALKNC GHU37060 10 10 -

Motif 2 width=16
Element Seqn Id St Int Rpt
APIFLRLAFHDAANFN MCU43561 27 0 -
APIMVRLAWHSAGTFD APX1_ARATH 32 0 -
APIMVRLAWHSAGTFD S43157 33 0 -
APLMLRLAWHSAGTYD NTU15933 33 0 -
APLILRLAWHSAGTFD A45116 33 0 -
APLMLRLAWHSAGTFD SOYASCPER 33 0 -
APIMLRLAWHDAGTYD GHU37060 31 0 -
APILLRLSFHDAVDYD SPIAP 34 0 -

Motif 3 width=11
Element Seqn Id St Int Rpt
TKTGGPFGTIK A45116 51 2 -
TKTGGPFGTIK SOYASCPER 51 2 -
TKTGGPNGSIR GHU37060 49 2 -
DKTGGVNGSLR MCU43561 45 2 -
TKRGGANGSVR SPIAP 52 2 -
SKTGGPFGTMR NTU15933 51 2 -
SRTGGPFGTMR S43157 51 2 -
SRTGGPFGTMR APX1_ARATH 50 2 -

Motif 4 width=25
Element Seqn Id St Int Rpt
ELAHSANNGLDIAVRLLEPLKAEFP SOYASCPER 65 3 -
EQAHGANSGIHIALRLLDPIREQFP APX1_ARATH 64 3 -
ELAHGANNGLHIALRLLEPIREQFP S43157 65 3 -
EQGHGANNGIDIAIRLLEPIKEQFP NTU15933 65 3 -
ELAHGANNGLDIAVRLLEPIKEQFP A45116 65 3 -
EFTHGANSGLKIAIDFCEEVKAKHP GHU37060 63 3 -
ELGQPPNGGIKVGIDLIEEVKKKHP MCU43561 59 3 -
ELNRTPNKGIETAVRFCEPIKRRHP SPIAP 66 3 -

Motif 5 width=19
Element Seqn Id St Int Rpt
DITYADLYQLAGIVAVEVT SPIAP 91 0 -
TVSYADLYQLAGVVAVGAS MCU43561 84 0 -
ILSYADFYQLAGVVAVEVT SOYASCPER 90 0 -
IVSYADFYQLAGVVAVEIT A45116 90 0 -
ILSYGDFYQLAGVVAVEVT NTU15933 90 0 -
TISHADFHQLAGVVAVEVT S43157 90 0 -
TISFADFHQLAGVVAVEVT APX1_ARATH 89 0 -
KITYADLYQLAGVVAVEVT GHU37060 88 0 -

Motif 6 width=13
Element Seqn Id St Int Rpt
GGPDIPFHPGRED APX1_ARATH 108 0 -
GGPEIPFHPGRED S43157 109 0 -
GGPDVPFHPGRED NTU15933 109 0 -
GGPEVPFHPGRED A45116 109 0 -
GGPEVPFHPGRED SOYASCPER 109 0 -
GGPTIDFVPGRKD GHU37060 107 0 -
GGPAIFFVPGRKD MCU43561 103 0 -
GGPAIDADVADQD SPIAP 110 0 -

Motif 7 width=18
Element Seqn Id St Int Rpt
RLPDATKGSDHLRDVFGK A45116 130 8 -
RLPDATKACDHLRQVFLK S43157 130 8 -
RLPDATKGSDHLRDVFVK NTU15933 130 8 -
RLPDATKGSDHLRDVFGK SOYASCPER 130 8 -
RLPDAKRGAPHLRDIFYR GHU37060 128 8 -
NIPNPNGGADHLRTVFHQ MCU43561 123 7 -
NIPNPRRGADHLRTVFYR SPIAP 123 0 -
RLPDATKGCDHLRDVFAK APX1_ARATH 129 8 -

Motif 8 width=22
Element Seqn Id St Int Rpt
MGLSDKDIVALSGAHTLGRCHK APX1_ARATH 148 1 -
MGLTDQDIVALSGAHTLGRCHK S43157 149 1 -
MGLSDKDIVALSGGHTLGRCHK NTU15933 149 1 -
MGLSDQDIVALSGGHTIGAAHK A45116 149 1 -
MGLTDQDIVALSGGHTIGAAHK SOYASCPER 149 1 -
MGLSDKDIVALSGGHTLGRAHP GHU37060 146 0 -
MGLVDKDIVTLSGAHTLGRAHS MCU43561 141 0 -
MGLNDKDIVVLSGAHALGGAHK SPIAP 141 0 -

Motif 9 width=25
Element Seqn Id St Int Rpt
DRSGFEGAWTSNPLIFDNSYFKELL S43157 171 0 -
ERSGFEGPWTTNPLIFDNSYFTELL NTU15933 171 0 -
ERSGFEGPWTSNPLIFDNSYFTELL A45116 171 0 -
ERSGFEGPWTSNPLIFDNSYFTELL SOYASCPER 171 0 -
ERSGFDGPWTNEPLKFDNSYFLELL GHU37060 168 0 -
NISGFDGPFTREPLKFDNSYYVELL MCU43561 163 0 -
DRSGFDGDFTRNPLTFDNSYFVELL SPIAP 163 0 -
DRSGFEGAWTSNPLIFDNSYFKELL APX1_ARATH 170 0 -

Motif 10 width=26
Element Seqn Id St Int Rpt
GESEGLLKLPTDKALLDDPEFRKYVE GHU37060 194 1 -
GEKEGLLQLPSDKALLSDPVFRPLVD SOYASCPER 197 1 -
GEKDGLLQLPSDKALLTDSVFRPLVE A45116 197 1 -
GDTPGLVKFPTDKALLTDPRFRPFVD SPIAP 189 1 -
GEKEGLLQLPSDKALLSDPAFRPLVE NTU15933 197 1 -
GEKEGLLQLPSDKALLDDPVFRPLVE S43157 197 1 -
GEKEGLLQLVSDKALLDDPVFRPLVE APX1_ARATH 196 1 -
GDTEGLVKFPTDKVLLQDDVFRPLVE MCU43561 189 1 -

Motif 11 width=25
Element Seqn Id St Int Rpt
KYAADEDAFFADYAEAHLKLSELGF NTU15933 223 0 -
KYAADEDAFFADYAEAHQKLSELGF SOYASCPER 223 0 -
LYAKDEDAFFRDYAESHKKLSELGF GHU37060 220 0 -
IYAKHQDAFFRDYAESHKKMSELGF MCU43561 215 0 -
LYARDQRAFFRDYAESHKKMSLLGL SPIAP 215 0 -
KYAADEEAFFADYAEAHLKLSELGF S43157 223 0 -
KYAADEDAFFADYAEAHMKLSELGF APX1_ARATH 222 0 -
KYAADEDVFFADYAEAHLKLSELGF A45116 223 0 -
Final Motifs
Motif 1  width=21
Element Seqn Id St Int Rpt
YQKAIEKAKRKLRGFIAEKNC Q96399 12 12 -
YLKAVDKCKRKLRGLIAEKNC Q40589 12 12 -
YLKAVDKCKRKLRGLIAEKNC Q42941 12 12 -
YLKAVDKCKRKLRGLIAEKNC Q42661 12 12 -
YKKAIDKAKRKLRGLIAEKNC O48919 12 12 -
YKKAIDKAKRKLRGLIAEKNC O49159 12 12 -
YQKAIEKAKKKLRGFIAEKRC Q41712 12 12 -
YQKAVEKAKKKLRGFIAEKRC Q39843 12 12 -
YQKAVEKAKKKLRGFIAEKRC Q43758 12 12 -
YQTAVGKAKRKLRALIAEKNC O65161 12 12 -
YQKAIEKAKRKLRGFIAEKKC APX1_PEA 11 11 -
YLKAVDKAKRKLRGLIAEKNC Q41772 12 12 -
YQKSIEKARRKLRGLIAEKQC Q42459 12 12 -
YQKEIEKCKRKLRGLIAEKNC Q43824 12 12 -
YKKAVEKCRRKLRGLIAEKNC APX1_ARATH 11 11 -
YQEAVEKARQKLRALIAEKSC P93404 12 12 -
YQKAIEKCKRKLRGLIAEKNC P93657 12 12 -
YKKAVQRCKRKLRGLIAEKHC Q39006 13 13 -
YQKVYEKCKRKLRGLIAEKNC O49122 12 12 -
YLKEITKARRELRSLIANKNC Q42564 10 10 -
YLKEIDKARRDLRALIALKNC Q39780 10 10 -
YLKEITKARRELRSLIANKNC O81810 10 10 -
YLKEIEKTRRDLRALISSRNC O65634 9 9 -
YLKHIEGARKELRTIISTKQC O81603 10 10 -
YMEAIEGARRDLVALVQSKNC Q42909 6 6 -
YLKDLEGARRDLASIIQRKNA O81604 11 11 -

Motif 2 width=16
Element Seqn Id St Int Rpt
APLMLRLAWHSAGTFC Q96399 33 0 -
APLMLRLAWHSAGTYD Q40589 33 0 -
APLMLRLAWHSAGTYD Q42941 33 0 -
APLMLRLAWHSAGTYD Q42661 33 0 -
APLMLRLAWHSAGTYD O48919 33 0 -
APLMLRLAWHSAGTYD O49159 33 0 -
APLMLRLAWHSAGTFD Q41712 33 0 -
APLMLRLAWHSAGTYD Q39843 33 0 -
APLMLRLAWHSAGTFD Q43758 33 0 -
APLMLRLAWHSAGTYD O65161 33 0 -
APLILRLAWHSAGTFD APX1_PEA 32 0 -
APLMLRLAWHSVGTFD Q41772 33 0 -
APLMLRLAWHSAGTFD Q42459 33 0 -
APIMVRLAWHSAGTFD Q43824 33 0 -
APIMVRLAWHSAGTFD APX1_ARATH 32 0 -
APLMLRLAWHSAGTFD P93404 33 0 -
APIMVRLAWHSAGTFD P93657 33 0 -
APIVLRLAWHSAGTFD Q39006 34 0 -
APIMVRLAWHSAGTFD O49122 33 0 -
APIMLRLAWHDAGTYD Q42564 31 0 -
APIMLRLAWHDAGTYD Q39780 31 0 -
APIMLRLAWHDAGTYD O81810 31 0 -
APIMLRLAWHDAGTYD O65634 30 0 -
APLMLRLSFHDAGTYD O81603 31 0 -
APIFLRLAFHDAANFN Q42909 27 0 -
APVLLRLAFHDAANYN O81604 32 0 -

Motif 3 width=11
Element Seqn Id St Int Rpt
SKTGGPFGTMR Q96399 51 2 -
SKTGGPFGTMR Q40589 51 2 -
SKTGGPFGTMR Q42941 51 2 -
SKTGGPFGTMR Q42661 51 2 -
TKTGGPFGTMK O48919 51 2 -
TKTGGPFGTMK O49159 51 2 -
TKTGGPFGTIK Q41712 51 2 -
SKTGGPFGTIK Q39843 51 2 -
TKTGGPFGTIK Q43758 51 2 -
TRTGGPFGTMR O65161 51 2 -
TKTGGPFGTIK APX1_PEA 50 2 -
TKTGGPFGTMK Q41772 51 2 -
SKTGGPFGTMK Q42459 51 2 -
SRTGGPFGTMR Q43824 51 2 -
SRTGGPFGTMR APX1_ARATH 50 2 -
SKTGGPFGTMK P93404 51 2 -
SRTGVPFGTMR P93657 51 2 -
TKTGGPFGTIR Q39006 52 2 -
SRTGVPFGTMR O49122 51 2 -
SKTGGPNGSIR Q42564 49 2 -
TKTGGPNGSIR Q39780 49 2 -
SKTGGPNGSIR O81810 49 2 -
KKTGGANGSIR O65634 48 2 -
TKKGGPNGTVR O81603 49 2 -
DKTGGVNGSLR Q42909 45 2 -
NNTGGVNGSVR O81604 50 2 -

Motif 4 width=25
Element Seqn Id St Int Rpt
ELAHGANNGLDIAVRLLEPIKEQFP Q96399 65 3 -
EQGHGANNGIDIAIRLLEPIKEQFP Q40589 65 3 -
EQGHGANNGIDIAIRLLEPIKEQFP Q42941 65 3 -
EQSHGANNGIDIALRLLEPLGEQFP Q42661 65 3 -
ELAHGANNGLDIAVRLLEPIKEQFP O48919 65 3 -
ELAHGANNGLDIAVRLLEPIKEQFP O49159 65 3 -
ELAHGANNGLDIAVRLLEPIKAEFP Q41712 65 3 -
ELAHGANNGLDIAVRLLEPLKAEFP Q39843 65 3 -
ELAHSANNGLDIAVRLLEPLKAEFP Q43758 65 3 -
ELAHGANNGIDIAVRLLEPIKEQFP O65161 65 3 -
ELAHGANNGLDIAVRLLEPIKEQFP APX1_PEA 64 3 -
EQAHGANAGLEIAIRLLEPIKEQFP Q41772 65 3 -
ELAHGANNGLVIAVRLLEPIKEQFP Q42459 65 3 -
ELAHGANNGLHIALRLLEPIREQFP Q43824 65 3 -
EQAHGANSGIHIALRLLDPIREQFP APX1_ARATH 64 3 -
ELSHAANAGLDIAVRMLEPIKEEIP P93404 65 3 -
ELAHGANSGLHIALRLLEPIREQFP P93657 65 3 -
ELAHDANNGLDIAVRLLDPIKELFP Q39006 66 3 -
ELAHGANSGLHIALRLLEPIREQFP O49122 65 3 -
EHTHGANSGLKIALDLCEGVKAKHP Q42564 63 3 -
EFTHGANSGLKIAIDFCEEVKAKHP Q39780 63 3 -
EHTHGANSGLKIALDLCEGVKAKHP O81810 63 3 -
ELNRPHNKGLEKAVAFCEEVKAKHP O65634 62 3 -
ELNNPANNGIKTAVDLVEQVKVKHP O81603 61 1 -
ELGQPPNGGIKVGIDLIEEVKKKHP Q42909 59 3 -
ELSQPPNKGIEDGVKFCEEVKKKHP O81604 64 3 -

Motif 5 width=19
Element Seqn Id St Int Rpt
ILSYADFYQLAGVVAVEVT Q96399 90 0 -
ILSYGDFYQLAGVVAVEVT Q40589 90 0 -
ILSYGDFYQLAGVVAVEVT Q42941 90 0 -
ILSYADFYQLAGVVAVEVT Q42661 90 0 -
ILSYADFYQLAGVVAVEVT O48919 90 0 -
ILSYADFYQLAGVVAVEVT O49159 90 0 -
ILSYADFYQLAGVVAVEVT Q41712 90 0 -
ILTYADFYQLAGVVAVEVT Q39843 90 0 -
ILSYADFYQLAGVVAVEVT Q43758 90 0 -
ILSYADFYQLAGVVAVEVT O65161 90 0 -
IVSYADFYQLAGVVAVEIT APX1_PEA 89 0 -
ILSYADFYQLAGVVAVEVT Q41772 90 0 -
EITYADFYQLAEFVAVEVT Q42459 90 0 -
TISHADFHQLAGVVAVEVT Q43824 90 0 -
TISFADFHQLAGVVAVEVT APX1_ARATH 89 0 -
TISYADFYQLAGVVAVEVS P93404 90 0 -
TISHADFHQLAGVVAVEVT P93657 90 0 -
ILSYADFYQLAGVVAVEIT Q39006 91 0 -
TISFADFHQLAGVVAVEVT O49122 90 0 -
KITYADLYQLAGVVAVEVT Q42564 88 0 -
KITYADLYQLAGVVAVEVT Q39780 88 0 -
KITYADLYQLAGVVAVEVT O81810 88 0 -
RVSYADLYQLAGVVAVEVT O65634 87 0 -
KVTYADLYQLAGVVAVEVT O81603 86 0 -
TVSYADLYQLAGVVAVGAS Q42909 84 0 -
RVTYADIIQLAGVLAVELS O81604 89 0 -

Motif 6 width=13
Element Seqn Id St Int Rpt
GGPDVPFHPGRED Q96399 109 0 -
GGPDVPFHPGRED Q40589 109 0 -
GGPDVPFHPGRED Q42941 109 0 -
GGPDVPFHPGRED Q42661 109 0 -
GGPDVPFHPGRED O48919 109 0 -
GGPDVPFHPGRED O49159 109 0 -
GGPEVPFHPGRED Q41712 109 0 -
GGPEVPFHPGRED Q39843 109 0 -
GGPEVPFHPGRED Q43758 109 0 -
GGPEIPFHPGRED O65161 109 0 -
GGPEVPFHPGRED APX1_PEA 108 0 -
GGPDVPFHPGRQD Q41772 109 0 -
GGPEVPFHPGRED Q42459 109 0 -
GGPEIPFHPGRED Q43824 109 0 -
GGPDIPFHPGRED APX1_ARATH 108 0 -
GGPAVPFHPGRED P93404 109 0 -
GGPEIPFHPGRED P93657 109 0 -
GGPEIPFHPGRLD Q39006 110 0 -
GGPDIPFHPGRED O49122 109 0 -
GGPDIVFVPGRKD Q42564 107 0 -
GGPTIDFVPGRKD Q39780 107 0 -
GGPDIVFVPGRKD O81810 107 0 -
GGPAIPFTPGRKD O65634 106 0 -
GGPVINFVPGRPD O81603 105 0 -
GGPAIFFVPGRKD Q42909 103 0 -
GGPCIDFVPGRMD O81604 108 0 -

Motif 7 width=18
Element Seqn Id St Int Rpt
RLPDATKGSDHLRDVFYT Q96399 130 8 -
RLPDATKGSDHLRDVFVK Q40589 130 8 -
RLPDATKGSDHLRDVFVK Q42941 130 8 -
RLPDATKGSDHLRDVFVK Q42661 130 8 -
RLPDAGKGSDHLREVFGK O48919 130 8 -
RLPDAGKGSDHLREVFGK O49159 130 8 -
RLPDATKGSDHLRDVFGK Q41712 130 8 -
RLPDATKGSDHLRDVFGK Q39843 130 8 -
RLPDATKGSDHLRDVFGK Q43758 130 8 -
RLPDATKGSDHLRQVFSQ O65161 130 8 -
RLPDATKGSDHLRDVFGK APX1_PEA 129 8 -
RLPDATQGSDHLRQVFST Q41772 130 8 -
RLPDATKGCDHLRDVFIK Q42459 130 8 -
RLPDATKACDHLRQVFLK Q43824 130 8 -
RLPDATKGCDHLRDVFAK APX1_ARATH 129 8 -
RLPDATKGSDHLRQVFGA P93404 130 8 -
RLPDATKACDHLRQVFLK P93657 130 8 -
RLPQATKGVDHLRDVFGR Q39006 131 8 -
RLPDATKGCDHLRQVLLK O49122 130 8 -
RLPDAKQGFQHLRDVFYR Q42564 128 8 -
RLPDAKRGAPHLRDIFYR Q39780 128 8 -
RLPDAKQGFQHLRDVFYR O81810 128 8 -
ELPNPNEGASHLRTLFSR O65634 126 7 -
SLPLPSGDANHLREVFHR O81603 125 7 -
NIPNPNGGADHLRTVFHQ Q42909 123 7 -
NIPNPRGGADHLRRTFYQ O81604 128 7 -

Motif 8 width=22
Element Seqn Id St Int Rpt
MGLSDQDIVALSGGHTLGRAHK Q96399 148 0 -
MGLSDKDIVALSGGHTLGRCHK Q40589 149 1 -
MGLSDKDIVALSGGHTLGRCHK Q42941 149 1 -
MGLSDQDIVALSGGHTLGRCHK Q42661 149 1 -
MGLSDQDIVALSGGHTLGRAHK O48919 149 1 -
MGLSDQDIVALSGGHTLGRAHK O49159 149 1 -
MGLSDQDIVALSGGHTIGAAHK Q41712 149 1 -
MGLSDRDIVALSGGHTIGAAHK Q39843 149 1 -
MGLTDQDIVALSGGHTIGAAHK Q43758 149 1 -
MGLNDQDIVALSGAHTLGRCHK O65161 149 1 -
MGLSDQDIVALSGGHTIGAAHK APX1_PEA 148 1 -
MGLSDQDIVALSGGHTLGRCHK Q41772 149 1 -
MGLTDQDIVALSGGHTLGRCHK Q42459 149 1 -
MGLTDQDIVALSGAHTLGRCHK Q43824 149 1 -
MGLSDKDIVALSGAHTLGRCHK APX1_ARATH 148 1 -
MGLSDQDIVALSGGHTLGRCHK P93404 149 1 -
MVLTDQDIVALSGAHTLGRCHK P93657 149 1 -
MGLNDKDIVALSGGHTLGRCHK Q39006 149 0 -
MVLTDQDIVALSGAHTLGRYRA O49122 149 1 -
MGLSDKDIVALSGGHTLGRAHP Q42564 146 0 -
MGLSDKDIVALSGGHTLGRAHP Q39780 146 0 -
MGLSDKDIVALSGGHTLGRAHP O81810 146 0 -
MGLLDRDIVALSGGHTLGRAHK O65634 144 0 -
MGLSDRDIVVLSGAHTLGRANR O81603 143 0 -
MGLVDKDIVTLSGAHTLGRAHS Q42909 141 0 -
MGLSDKDIVVLSGAHTLGRARK O81604 146 0 -

Motif 9 width=25
Element Seqn Id St Int Rpt
ERSGFEGPWTTNPLIFDKSYFTELL Q96399 170 0 -
ERSGFEGPWTTNPLIFDNSYFTELL Q40589 171 0 -
ERSGFEGPWTTNPLIFDNSYFTELL Q42941 171 0 -
ERSGFEGPWTANPLIFDNSYFKELL Q42661 171 0 -
ERSGFEGPWTPNPLIFDNSYFTVLL O48919 171 0 -
ERSGFEGPWTPNPLIFDNSYFTVLL O49159 171 0 -
ERSGFEGPWTSNPLIFDNSYFKELL Q41712 171 0 -
ERSGFEGPWTSNPLIFDNSYFKELL Q39843 171 0 -
ERSGFEGPWTSNPLIFDNSYFTELL Q43758 171 0 -
ERSGFEGAWTTNPLIFDNSYFKELL O65161 171 0 -
ERSGFEGPWTSNPLIFDNSYFTELL APX1_PEA 170 0 -
DRSGFEGAWTSNPLIFDNSYFKELL Q41772 171 0 -
DRSGFEGAWTTNPLVFDNTYFKELL Q42459 171 0 -
DRSGFEGAWTSNPLIFDNSYFKELL Q43824 171 0 -
DRSGFEGAWTSNPLIFDNSYFKELL APX1_ARATH 170 0 -
ERSGFEGPWTRNPLQFDNSYFTELL P93404 171 0 -
DRSGFEGAWTSNPLIFDNSYFKELL P93657 171 0 -
ERSGFEGAWTPNPLIFDNSYFKEIL Q39006 171 0 -
APSGFEGAWTSNPLIFDNSYFKELL O49122 171 0 -
ERSGFDGPWTQEPLKFDNSYFVELL Q42564 168 0 -
ERSGFDGPWTNEPLKFDNSYFLELL Q39780 168 0 -
ERSGFDGPWTQEPLNFDNSYFVREL O81810 168 0 -
ERSDFEGPWTQDPLKFDNSYFVELL O65634 166 0 -
DRSGVDGPFTKNPLKFDNSYYVELL O81603 165 0 -
NISGFDGPFTREPLKFDNSYYVELL Q42909 163 0 -
ENSGFNGPFTRNTLKFDNSYFVELM O81604 168 0 -

Motif 10 width=26
Element Seqn Id St Int Rpt
GEKEGLLQLASDKALLSDPVFRPLVE Q96399 196 1 -
GEKEGLLQLPSDKALLSDPAFRPLVE Q40589 197 1 -
GEKEGLLQLPSDKALLSDPAFRPLVE Q42941 197 1 -
GEKEGLLQLPSDKALLSDPAFRPLVE Q42661 197 1 -
GEKEGLLQLPTDKALLSDPVFRPLVE O48919 197 1 -
GEKEGLLQLPTDKALLSDPVFRPLVE O49159 197 1 -
GEKEGLLQLPSDKALLSDPVFRPLVE Q41712 197 1 -
GEKEGLLQLPSDKALLSDPVFRPLVE Q39843 197 1 -
GEKEGLLQLPSDKALLSDPVFRPLVD Q43758 197 1 -
GEKEDLLQLPSDKALLSDPVFRPLVE O65161 197 1 -
GEKDGLLQLPSDKALLTDSVFRPLVE APX1_PEA 196 1 -
GEKEGLLQLPSDKALLSDPSFRPLVD Q41772 197 1 -
GEKEGLLQLPSDKALLSDPVFRPLVE Q42459 197 1 -
GEKEGLLQLPSDKALLDDPVFRPLVE Q43824 197 1 -
GEKEGLLQLVSDKALLDDPVFRPLVE APX1_ARATH 196 1 -
GDKEGLLQLPSDKALLSDPAFRPLVE P93404 197 1 -
GEKEGLLQLPSDKALLDEPVFRPLVE P93657 197 1 -
GEKEGLLQLPTDKALLDDPLFLPFVE Q39006 197 1 -
GEKEGLLQLVSDKALLDDPVFRPLVE O49122 197 1 -
GESEGLLKLPTDKTLLEDPEFRRLVE Q42564 194 1 -
GESEGLLKLPTDKALLDDPEFRKYVE Q39780 194 1 -
GESEGLLKLPTDKTLLEDPEFRRLVE O81810 195 2 -
GETPGLLQLKTDKALLDDPKFHPFVK O65634 192 1 -
GDTPELVKFNTDKVLLQDPTFRKYVQ O81603 191 1 -
GDTEGLVKFPTDKVLLQDDVFRPLVE Q42909 189 1 -
GETPGLVKFPTDKALVQDPVFRPLVE O81604 194 1 -

Motif 11 width=25
Element Seqn Id St Int Rpt
KYAADEDAFFADYAEAHQKLSELGF Q96399 222 0 -
KYAADEDAFFADYAEAHLKLSELGF Q40589 223 0 -
KYAADEDAFFADYAEAHLKLSELGF Q42941 223 0 -
KYAADEDAFFADYAEAHLKLSELGF Q42661 223 0 -
KYAADEDAFFADYALAHQRLSELGF O48919 223 0 -
KYAADEDAFFSDYALAHQRLSELGF O49159 223 0 -
KYAADEDAFFADYAVAHQKLSELGF Q41712 223 0 -
KYASDEDAFFADYAEAHQKLSELGF Q39843 223 0 -
KYAADEDAFFADYAEAHQKLSELGF Q43758 223 0 -
KYAADEDAFFADYTEAHLKLSELGF O65161 223 0 -
KYAADEDVFFADYAEAHLKLSELGF APX1_PEA 222 0 -
KYAADEDAFFADYAEAHLKLSELGF Q41772 223 0 -
KYAADEDAFFADYAEAHLKLSELGF Q42459 223 0 -
KYAADEEAFFADYAEAHLKLSELGF Q43824 223 0 -
KYAADEDAFFADYAEAHMKLSELGF APX1_ARATH 222 0 -
KYAADEKAFFEDYKEAHLKLSELGF P93404 223 0 -
KYAADEEAFFADYAEAHLKLSELGF P93657 223 0 -
KYAADEDASFEDYTEAHLKLSELGF Q39006 223 0 -
KYAADEEAFFADYAEAHLKLSELGF O49122 223 0 -
LYAKDEDAFFRDYAESHKKLSELGF Q42564 220 0 -
LYAKDEDAFFRDYAESHKKLSELGF Q39780 220 0 -
ELYADEDAFFRDYAESHKKLSELGF O81810 220 -1 -
LYAKDEDMFFKAYAISHKKLSELGF O65634 218 0 -
LYAKDEKAFLTHYAESHKKMSELGK O81603 217 0 -
IYAKHQDAFFRDYAESHKKMSELGF Q42909 215 0 -
LYARHEGAFFRDYAESHKKLSELGF O81604 220 0 -