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PR00448

Identifier
NSFATTACHMNT  [View Relations]  [View Alignment]  
Accession
PR00448
No. of Motifs
7
Creation Date
14-DEC-1995  (UPDATE 22-JUN-1999)
Title
NSF attachment protein signature
Database References

INTERPRO; IPR000744
Literature References
1. ORDWAY, R.W., PALLANCK, L. AND GANETZKY, B.
A TPR domain in the SNAP secretory proteins.
TRENDS BIOCHEM.SCI. 19 530-531 (1994).

Documentation
The `secretory complex' comprises a family of conserved proteins, members
of which appear to function at all sites of constitutive and regulated
secretion in eukaryotes [1]. Among them are 2 types of cytosolic protein:
NSF (N-ethyl-maleimide-sensitive protein) and the SNAPs (alpha-, beta- and
gamma-soluble NSF attachment proteins). The yeast vesicular fusion protein,
sec17, a cytoplasmic peripheral membrane protein involved in vesicular
transport between the endoplasmic reticulum and the golgi apparatus, shows
a high degree of sequence similarity to the alpha-SNAP family.
 
NSFATTACHMNT is a 7-element fingerprint that provides a signature for NSF
and sec17 proteins. The fingerprint was derived from an initial alignment
of 6 sequences: the motifs were drawn from conserved regions spanning the
full alignment length. Two iterations on OWL26.3 were required to reach
convergence, at which point a true set comprising 7 sequences was
identified. Three partial matches were also found: BI47_MOUSE is a
fragment lacking the first motif; and DHYC_ANTCR and DHYC_TRIGR are 
dynein beta chains.  
 
An update on SPTR37_9f identified a true set of 7 sequences, and 4
partial matches.
Summary Information
   7 codes involving  7 elements
2 codes involving 6 elements
0 codes involving 5 elements
2 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
77777777
61212222
50000000
41012202
30000000
20000000
1234567
True Positives
Q23983        Q25391        SC17_YEAST    SNAA_BOVIN    
SNAA_HUMAN SNAA_RAT SNAB_BOVIN
True Positive Partials
Codes involving 6 elements
P93798 Q18921
Codes involving 4 elements
O90758 P78603
Sequence Titles
Q23983      SOLUBLE NSF ATTACHMENT PROTEIN - DROSOPHILA MELANOGASTER (FRUIT FLY). 
Q25391 SNAP-TYPE PROTEIN - LOLIGO PEALEII (LONGFIN SQUID).
SC17_YEAST VESICULAR-FUSION PROTEIN SEC17 - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
SNAA_BOVIN ALPHA-SOLUBLE NSF ATTACHMENT PROTEIN (SNAP-ALPHA) - BOS TAURUS (BOVINE).
SNAA_HUMAN ALPHA-SOLUBLE NSF ATTACHMENT PROTEIN (SNAP-ALPHA) - HOMO SAPIENS (HUMAN).
SNAA_RAT ALPHA-SOLUBLE NSF ATTACHMENT PROTEIN (SNAP-ALPHA) - RATTUS NORVEGICUS (RAT).
SNAB_BOVIN BETA-SOLUBLE NSF ATTACHMENT PROTEIN (SNAP-BETA) - BOS TAURUS (BOVINE).

P93798 SOLUBLE NSF ATTACHMENT PROTEIN HOMOLOGUE, COMPLETE CDS - VITIS VINIFERA (GRAPE).
Q18921 SIMILAR TO ALPHA-SNAP PROTEIN - CAENORHABDITIS ELEGANS.

O90758 SNAP - FOWLPOX VIRUS.
P78603 SEC17-LIKE PROTEIN - COPRINUS CINEREUS (INKY CAP FUNGUS).
Scan History
OWL26_3    2  50   NSINGLE    
SPTR37_9f 1 50 NSINGLE
Initial Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
KFEEAADLCVQAATIYRLRK SCIIL26O13 32 32 -
KFEEAADLCVQAATIYRLRK SC17_YEAST 32 32 -
KIEEACEIYARAANMFKMAK RNSNAPGEN 37 37 -
KIEEACEIYARAANMFKMAK S32367 37 37 -
RIEEACEMYTRAANMFKMAK S32368 37 37 -
KVEDAIECYQRAGNMFKMSK DMU09374 35 35 -

Motif 2 width=18
Element Seqn Id St Int Rpt
AINCLMRAIEIYTDMGRF RNSNAPGEN 100 43 -
AINCLNAAIDIYTDMGRF S32368 100 43 -
AVNCLMKSIDIYTDMGRF DMU09374 98 43 -
AVDSLENAIQIFTHRGQF SCIIL26O13 96 44 -
AVDSLENPIQIFTHRGQF SC17_YEAST 96 44 -
AINCLMRAIEIYTDMGRF S32367 100 43 -

Motif 3 width=18
Element Seqn Id St Int Rpt
EIYETELVDIEKAIAHYE S32368 129 11 -
EMYESDPNNLAKSIQHYE DMU09374 127 11 -
EILENDLHDYAKAIDCYE SCIIL26O13 125 11 -
EILENDLHDYAKAIDCYE SC17_YEAST 125 11 -
EIYETELVDIEKAIAHYE S32367 129 11 -
EIYETELVDVEKAIAHYE RNSNAPGEN 129 11 -

Motif 4 width=24
Element Seqn Id St Int Rpt
NKCMLKVAQYAAQLEDYEKAISIY DMU09374 160 15 -
NKCFIKCADLKALDGQYIEASDIY SC17_YEAST 158 15 -
NKCFIKCADLKALDGQYIEASDIY SCIIL26O13 158 15 -
NKCLLKVAGYAAQLEQYQKAIDIY S32367 162 15 -
NKCLLKVAGYAAQLEQYQKAIDIY RNSNAPGEN 162 15 -
NKCLLKVAAYAAQLEQYQKAIEIF S32368 162 15 -

Motif 5 width=20
Element Seqn Id St Int Rpt
MDSPLLKYSAKDYFFKAALX RNSNAPGEN 193 7 -
MDSPLLKYSAKDYFFKAALC S32367 193 7 -
MDNPLLKYSAKDYFFKAALC S32368 193 7 -
LESSLLKYSAKEYFFRAALC DMU09374 191 7 -
MGNRLSQWSLKDYFLKKGLC SCIIL26O13 189 7 -
MGNRLSQWSLKDYFLKKGLC SC17_YEAST 189 7 -

Motif 6 width=21
Element Seqn Id St Int Rpt
PNFADSRESNFLKSLIDAVNE SCIIL26O13 230 21 -
PAFQDSREFKLIKVLCENLEE DMU09374 231 20 -
PAFTDSRECKLLKKLLEAHEE S32368 233 20 -
PAFSDSRECKLMKKLLEAHEE RNSNAPGEN 233 20 -
PAFSDSRECKRIKKLLEAHEE S32367 233 20 -
PNFADSRESNFLKSLIDAVNE SC17_YEAST 229 20 -

Motif 7 width=21
Element Seqn Id St Int Rpt
KEYDSISRLDQWLTTMLLRIK S32367 264 10 -
KEYDSISRLDQWLTTMLLRIK RNSNAPGEN 264 10 -
KEFDSISRLDQWLTTMLLRIK S32368 264 10 -
KDYDSISRLDQWYTTILLRIK DMU09374 262 10 -
KEFDNFMRLDKWKITILNKIK SCIIL26O13 261 10 -
KEFDNFMRLDKWKITILNKIK SC17_YEAST 260 10 -
Final Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
KIEEACEIYARAANMFKMAK SNAA_BOVIN 37 37 -
KIEEACEIYARAANMFKMAK SNAA_RAT 37 37 -
RIEEACEMYTRAANMFKMAK SNAB_BOVIN 37 37 -
KIEEACEIYARAANMFKMAK SNAA_HUMAN 37 37 -
KTEEAAELFVRAANTFKMAK Q25391 35 35 -
KVEDAIECYQRAGNMFKMSK Q23983 35 35 -
KFEEAADLCVQAATIYRLRK SC17_YEAST 32 32 -

Motif 2 width=18
Element Seqn Id St Int Rpt
AINCLMRAIEIYTDMGRF SNAA_BOVIN 100 43 -
AINCLMRAIEIYTDMGRF SNAA_RAT 100 43 -
AINCLNAAIDIYTDMGRF SNAB_BOVIN 100 43 -
AINCLMRAIEIYTDMGRF SNAA_HUMAN 100 43 -
SINCLLKAVEIYTDMGRF Q25391 98 43 -
AVNCLMKSIDIYTDMGRF Q23983 98 43 -
AVDSLENPIQIFTHRGQF SC17_YEAST 96 44 -

Motif 3 width=18
Element Seqn Id St Int Rpt
EIYETELVDIEKAIAHYE SNAA_BOVIN 129 11 -
EIYETELVDVEKAIAHYE SNAA_RAT 129 11 -
EIYETELVDIEKAIAHYE SNAB_BOVIN 129 11 -
EIYEIELVDIEKAIAHYE SNAA_HUMAN 129 11 -
EIYENELADIEQAMSHYE Q25391 127 11 -
EMYESDPNNLAKSIQHYE Q23983 127 11 -
EILENDLHDYAKAIDCYE SC17_YEAST 125 11 -

Motif 4 width=24
Element Seqn Id St Int Rpt
NKCLLKVAGYAAQLEQYQKAIDIY SNAA_BOVIN 162 15 -
NKCLLKVAGYAAQLEQYQKAIDIY SNAA_RAT 162 15 -
NKCLLKVAAYAAQLEQYQKAIEIF SNAB_BOVIN 162 15 -
NKCLLKVAGYAALLEQYQKAIDIY SNAA_HUMAN 162 15 -
NKCFLKVAQYSAQLEQYQKAIDIY Q25391 160 15 -
NKCMLKVAQYAAQLEDYEKAISIY Q23983 160 15 -
NKCFIKCADLKALDGQYIEASDIY SC17_YEAST 158 15 -

Motif 5 width=20
Element Seqn Id St Int Rpt
MDSPLLKYSAKDYFFKAALC SNAA_BOVIN 193 7 -
MDSPLLKYSAKDYFFKAALX SNAA_RAT 193 7 -
MDNPLLKYSAKDYFFKAALC SNAB_BOVIN 193 7 -
MDTPLLKYSAKDYFFKAALC SNAA_HUMAN 193 7 -
MENALLKYSAKDHFFRAALC Q25391 191 7 -
LESSLLKYSAKEYFFRAALC Q23983 191 7 -
MGNRLSQWSLKDYFLKKGLC SC17_YEAST 189 7 -

Motif 6 width=21
Element Seqn Id St Int Rpt
PAFSDSRECKRIKKLLEAHEE SNAA_BOVIN 233 20 -
PAFSDSRECKLMKKLLEAHEE SNAA_RAT 233 20 -
PAFTDSRECKLLKKLLEAHEE SNAB_BOVIN 233 20 -
PAFSDSRECKLMKKLLEAHEE SNAA_HUMAN 233 20 -
PAFSDSRECNLLKSVLDSMDN Q25391 231 20 -
PAFQDSREFKLIKVLCENLEE Q23983 231 20 -
PNFADSRESNFLKSLIDAVNE SC17_YEAST 229 20 -

Motif 7 width=21
Element Seqn Id St Int Rpt
KEYDSISRLDQWLTTMLLRIK SNAA_BOVIN 264 10 -
KEYDSISRLDQWLTTMLLRIK SNAA_RAT 264 10 -
KEFDSISRLDQWLTTMLLRIK SNAB_BOVIN 264 10 -
KEYDSISRLDQWLTTMLLRIK SNAA_HUMAN 264 10 -
ADYDSISRLDQWLTMMFLRIK Q25391 260 8 -
KDYDSISRLDQWYTTILLRIK Q23983 262 10 -
KEFDNFMRLDKWKITILNKIK SC17_YEAST 260 10 -