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PR00442

Identifier
GPROTEINAQ  [View Relations]  [View Alignment]  
Accession
PR00442
No. of Motifs
5
Creation Date
12-JUN-1995  (UPDATE 17-DEC-1999)
Title
G-protein alpha subunit group Q signature
Database References
PRINTS; PR00318 GPROTEINA
INTERPRO; IPR000654
PDB; 1GIA
SCOP; 1GIA
Literature References
1. LINDER, M.E. AND GILMAN, A.G.
G proteins.
SCI.AM. 267 56 (1992).
 
2. KAZIRO, Y., ITOH, H., KOZASA, T., NAKAFUKU, M. AND SATOH, T.
Structure and function of signal-transducing GTP-binding proteins.
ANNU.REV.BIOCHEMISTRY 60 349-400 (1991).
 
3. SIMON, M.I., STRATHMANN, M.P. AND GAUTAM, N.
Diversity of G proteins in signal transduction.
SCIENCE 252 802-808 (1993).

Documentation
Guanine nucleotide binding proteins (G proteins) are a family of membrane-
associated proteins that couple extracellularly-activated integral-membrane
receptors to intracellular effectors, such as ion channels and enzymes that
vary the concentration of second messenger molecules [1-3]. G proteins are
composed of 3 subunits (alpha, beta and gamma) which, in the resting state,
associate as a trimer at the inner face of the plasma membrane. The alpha
subunit has a molecule of guanosine diphosphate (GDP) bound to it:
stimulation of the G protein by an activated receptor leads to its exchange
for GTP (guanosine triphosphate). This results in the separation of the
alpha from the beta and gamma subunits, which always remain tightly
associated as a dimer. Both the alpha and beta-gamma subunits are then able
to interact with effectors, either individually or in a cooperative manner.
The intrinsic GTPase activity of the alpha subunit hydrolyses the bound GTP
to GDP. This returns the alpha subunit to its inactive conformation and
allows it to reassociate with the beta-gamma subunit, thus restoring the
system to its resting state.
 
G protein alpha subunits are 350-400 amino acids in length and have
molecular weights in the range 40-45 kDa. Seventeen distinct types of alpha
subunit have been identified in mammals. These fall into 4 main  groups on
the basis of both sequence similarity and function: alpha-S, alpha-Q,
alpha-I and alpha-12 [3]. Many alpha subunits are substrates for
ADP-ribosylation by cholera or pertussis toxins. They are often N-terminally
acylated, usually with myristate and/or palmitoylate, and these fatty acid
modifications are probably important for membrane association and high-
affinity interactions with other proteins. The atomic structure of the 
alpha subunit of the G protein involved in mammalian vision, transducin,
has been elucidated in both GTP- and GDB-bound forms, and shows considerable
similarity in both primary and tertiary structure in the nucleotide-binding
regions to other G proteins, such as p21-ras and EF-Tu.
 
The G protein alpha-Q (G alpha-Q) class are widely expressed, toxin
insensitive proteins that couple receptors, such as the M1 acetylcholine
receptor, to the effector enzyme phospholipase C (PLC), causing subsequent
InsP3-mediated release of calcium from internal stores. Alpha-Q subunits
found in squid and Drosophila are believed to be involved in signal trans-
duction, which is thought to be an InsP3-mediated process in invertebrates.
 
GPROTEINAQ is a 5-element fingerprint that provides a signature for the
G protein alpha subunit group Q. The fingerprint was derived from an initial
alignment of 6 sequences: the motifs were drawn from conserved regions
spanning virtually the full alignment length, focusing on those sections 
that characterise group Q alpha subunits but distinguish them from other
G protein alpha subtypes. Two iterations on SPTR37_10f were required to 
reach convergence, at which point a true set comprising 22 sequences was
identified. Three partial matches were also found: GB15_MOUSE, GB15_HUMAN 
and O88302 are members of the G15 group of alpha-Q proteins, which show a
high degree of divergence from other family members.
Summary Information
  22 codes involving  5 elements
0 codes involving 4 elements
2 codes involving 3 elements
1 codes involving 2 elements
Composite Feature Index
52222222222
400000
322020
201010
12345
True Positives
GB11_BOVIN    GB11_HUMAN    GB11_MELGA    GB11_MOUSE    
GB11_XENLA GB14_BOVIN GB14_MOUSE GBQ1_DROME
GBQ3_DROME GBQ_CANFA GBQ_HOMAM GBQ_HUMAN
GBQ_LOLFO GBQ_LYMST GBQ_MOUSE GBQ_PATYE
GBQ_XENLA O73819 O95837 P91955
Q17386 Q61939
True Positive Partials
Codes involving 3 elements
GB15_MOUSE O88302
Codes involving 2 elements
GB15_HUMAN
Sequence Titles
GB11_BOVIN  GUANINE NUCLEOTIDE-BINDING PROTEIN, ALPHA-11 SUBUNIT (GL2) - BOS TAURUS (BOVINE) 
GB11_HUMAN GUANINE NUCLEOTIDE-BINDING PROTEIN G(Y), ALPHA SUBUNIT (ALPHA-11) - HOMO SAPIENS
GB11_MELGA GUANINE NUCLEOTIDE-BINDING PROTEIN, ALPHA-11 SUBUNIT - MELEAGRIS GALLOPAVO (COMM
GB11_MOUSE GUANINE NUCLEOTIDE-BINDING PROTEIN, ALPHA-11 SUBUNIT - MUS MUSCULUS (MOUSE).
GB11_XENLA GUANINE NUCLEOTIDE-BINDING PROTEIN, ALPHA-11 SUBUNIT - XENOPUS LAEVIS (AFRICAN C
GB14_BOVIN GUANINE NUCLEOTIDE-BINDING PROTEIN, ALPHA-14 SUBUNIT (GL1) - BOS TAURUS (BOVINE)
GB14_MOUSE GUANINE NUCLEOTIDE-BINDING PROTEIN, ALPHA-14 SUBUNIT - MUS MUSCULUS (MOUSE).
GBQ1_DROME GUANINE NUCLEOTIDE-BINDING PROTEIN G(Q), ALPHA SUBUNIT ISOFORM 1 (DGQALPHA-1) -
GBQ3_DROME GUANINE NUCLEOTIDE-BINDING PROTEIN G(Q), ALPHA SUBUNIT ISOFORM 3 (DGQALPHA-3) -
GBQ_CANFA GUANINE NUCLEOTIDE-BINDING PROTEIN G(Q), ALPHA SUBUNIT - CANIS FAMILIARIS (DOG).
GBQ_HOMAM GUANINE NUCLEOTIDE-BINDING PROTEIN G(Q), ALPHA SUBUNIT - HOMARUS AMERICANUS (AME
GBQ_HUMAN GUANINE NUCLEOTIDE-BINDING PROTEIN G(Q), ALPHA SUBUNIT - HOMO SAPIENS (HUMAN).
GBQ_LOLFO GUANINE NUCLEOTIDE-BINDING PROTEIN G(Q), ALPHA SUBUNIT - LOLIGO FORBESI (NORTHER
GBQ_LYMST GUANINE NUCLEOTIDE-BINDING PROTEIN G(Q), ALPHA SUBUNIT - LYMNAEA STAGNALIS (GREA
GBQ_MOUSE GUANINE NUCLEOTIDE-BINDING PROTEIN G(Q), ALPHA SUBUNIT - MUS MUSCULUS (MOUSE).
GBQ_PATYE GUANINE NUCLEOTIDE-BINDING PROTEIN G(Q), ALPHA SUBUNIT - PATINOPECTEN YESSOENSIS
GBQ_XENLA GUANINE NUCLEOTIDE-BINDING PROTEIN G(Q), ALPHA SUBUNIT - XENOPUS LAEVIS (AFRICAN
O73819 GUANINE NUCLEOTIDE-BINDING PROTEIN, ALPHA-14 SUBUNIT - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
O95837 G-PROTEIN ALPHA SUBUNIT 14 - HOMO SAPIENS (HUMAN).
P91955 GQ PROTEIN ALPHA SUBUNIT - LIMULUS POLYPHEMUS (ATLANTIC HORSESHOE CRAB).
Q17386 EGL-30 - CAENORHABDITIS ELEGANS.
Q61939 GUANINE NUCLEOTIDE BINDING PROTEIN ALPHA 11 - MUS MUSCULUS (MOUSE).

GB15_MOUSE GUANINE NUCLEOTIDE-BINDING PROTEIN, ALPHA-15 SUBUNIT - MUS MUSCULUS (MOUSE).
O88302 GTP BINDING PROTEIN ALPHA 15 - RATTUS NORVEGICUS (RAT).

GB15_HUMAN GUANINE NUCLEOTIDE-BINDING PROTEIN, ALPHA-15 SUBUNIT (ALPHA-16) - HOMO SAPIENS (
Scan History
SPTR37_10f 2  30   NSINGLE    
Initial Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
CCLSEEAKEARRIND GBQ_MOUSE 3 3 -
CCLSDEVKESKRINA GB11_MOUSE 9 9 -
CCLSEEVKESKRINA GB11_XENLA 9 9 -
CCLSAEEKESQRISA GB14_MOUSE 5 5 -
CCLSEEAKEQKRINQ GBQ_LOLFO 3 3 -
CCLSEEAKEQKRINQ GBQ1_DROME 3 3 -

Motif 2 width=11
Element Seqn Id St Int Rpt
QNIFTAMQAMI GBQ_MOUSE 75 57 -
QNIFTAMQAMV GB11_MOUSE 81 57 -
QNIFTAMQSMI GB11_XENLA 81 57 -
QNIFTAMQAMI GB14_MOUSE 77 57 -
QNIFSAIQTLI GBQ_LOLFO 75 57 -
QNIFMAMQSMI GBQ1_DROME 75 57 -

Motif 3 width=11
Element Seqn Id St Int Rpt
ITYPWFQNSSV GBQ_MOUSE 253 167 -
ITYPWFQNSSV GB11_MOUSE 259 167 -
ITYPWFQNSSV GB11_XENLA 259 167 -
ITYPWFLNSSV GB14_MOUSE 255 167 -
ITYPWFQNSSV GBQ_LOLFO 254 168 -
ITYPWFQNSSV GBQ1_DROME 253 167 -

Motif 4 width=11
Element Seqn Id St Int Rpt
GPQRDAQAARE GBQ_MOUSE 291 27 -
GPQRDAQAARE GB11_MOUSE 297 27 -
GPQRDAATARE GB11_XENLA 297 27 -
GPKQDVKAARD GB14_MOUSE 293 27 -
GPKCDYEAARE GBQ_LOLFO 292 27 -
GPKQDHAAAKQ GBQ1_DROME 291 27 -

Motif 5 width=12
Element Seqn Id St Int Rpt
KMFVDLNPDSDK GBQ_MOUSE 305 3 -
KMFVDLNPDSDK GB11_MOUSE 311 3 -
KMFVDLNPDSDK GB11_XENLA 311 3 -
KLYQDQNPDKEK GB14_MOUSE 307 3 -
DSYMDLNEDKEK GBQ_LOLFO 306 3 -
KKYLACNPDPER GBQ1_DROME 305 3 -
Final Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
CCLSDEVKESKRINA GB11_HUMAN 9 9 -
CCLSDEVKESKRINA GB11_MELGA 9 9 -
CCLSEEAKEARRIND GBQ_CANFA 3 3 -
CCLSEEAKEARRIND GBQ_MOUSE 3 3 -
CCLSEEAKEARRIND GBQ_HUMAN 3 3 -
CCLSDEVKESKRINA GB11_MOUSE 9 9 -
CCLSDEVKESKRINA Q61939 9 9 -
CCLSDEVKESKRINA GB11_BOVIN 9 9 -
CCLSEEVKESKRINA GB11_XENLA 9 9 -
CCLSEEAEEARRIND GBQ_XENLA 3 3 -
CCLSEEAKEQKRINQ GBQ3_DROME 3 3 -
CCLSEEAKEQKRINQ GBQ_HOMAM 3 3 -
CCLSEEAKEQKRINC GBQ_PATYE 3 3 -
CCLSEEAREQKRINQ Q17386 3 3 -
CCLSEEGKEQKRINQ P91955 3 3 -
CCLSAEEKESQRINA O73819 4 4 -
CCLSAEEKESQRISA GB14_BOVIN 5 5 -
CCLSAEEKESQRISA GB14_MOUSE 5 5 -
CCLSAEEKESQRISA O95837 5 5 -
CCIPDELKEQKRINQ GBQ_LYMST 3 3 -
CCLSEEAKEQKRINQ GBQ_LOLFO 3 3 -
CCLSEEAKEQKRINQ GBQ1_DROME 3 3 -

Motif 2 width=11
Element Seqn Id St Int Rpt
QNIFTAMQAMI GB11_HUMAN 81 57 -
QNIFTAMQSMI GB11_MELGA 81 57 -
QNIFTAMQAMI GBQ_CANFA 75 57 -
QNIFTAMQAMI GBQ_MOUSE 75 57 -
QNIFTAMQAMI GBQ_HUMAN 75 57 -
QNIFTAMQAMV GB11_MOUSE 81 57 -
QNIFTAMQAMV Q61939 81 57 -
QNIFTAMQAMI GB11_BOVIN 81 57 -
QNIFTAMQSMI GB11_XENLA 81 57 -
QNIFSAMQAMI GBQ_XENLA 75 57 -
QNIFMAMQSMI GBQ3_DROME 75 57 -
QNIFMAMQSMI GBQ_HOMAM 75 57 -
QNIFMAMHSMI GBQ_PATYE 75 57 -
QNVFMAIQSMI Q17386 75 57 -
QNIIMAMQSMN P91955 75 57 -
QNIFTSMQSMI O73819 76 57 -
QNIFTAMQAMI GB14_BOVIN 77 57 -
QNIFTAMQAMI GB14_MOUSE 77 57 -
QNIFTAMQAMI O95837 77 57 -
QNIFMAMHAMI GBQ_LYMST 75 57 -
QNIFSAIQTLI GBQ_LOLFO 75 57 -
QNIFMAMQSMI GBQ1_DROME 75 57 -

Motif 3 width=11
Element Seqn Id St Int Rpt
ITYPWFQNSSV GB11_HUMAN 259 167 -
ITYPWFQNSSV GB11_MELGA 259 167 -
ITYPWFQNSSV GBQ_CANFA 253 167 -
ITYPWFQNSSV GBQ_MOUSE 253 167 -
ITYPWFQNSSV GBQ_HUMAN 253 167 -
ITYPWFQNSSV GB11_MOUSE 259 167 -
ITYPWFQNSSV Q61939 259 167 -
VTYPWFQNSSV GB11_BOVIN 259 167 -
ITYPWFQNSSV GB11_XENLA 259 167 -
ITYPWFQNSSV GBQ_XENLA 253 167 -
ITYPWFQNSSV GBQ3_DROME 253 167 -
ITYPWFQHSSV GBQ_HOMAM 253 167 -
ITYPWFQNSSV GBQ_PATYE 253 167 -
ITYPWFTNSSV Q17386 255 169 -
ITYPWFLNSSV P91955 253 167 -
ITYPWFQNSSV O73819 254 167 -
ITYPWFLNSSV GB14_BOVIN 255 167 -
ITYPWFLNSSV GB14_MOUSE 255 167 -
ITYPWFLNSSV O95837 255 167 -
ITYPWFQNSSV GBQ_LYMST 253 167 -
ITYPWFQNSSV GBQ_LOLFO 254 168 -
ITYPWFQNSSV GBQ1_DROME 253 167 -

Motif 4 width=11
Element Seqn Id St Int Rpt
GPQRDAQAARE GB11_HUMAN 297 27 -
GPQRDAQAARE GB11_MELGA 297 27 -
GPQRDAQAARE GBQ_CANFA 291 27 -
GPQRDAQAARE GBQ_MOUSE 291 27 -
GPQRDAQAARE GBQ_HUMAN 291 27 -
GPQRDAQAARE GB11_MOUSE 297 27 -
GPQRDAQAARE Q61939 297 27 -
GPQRDAQAARE GB11_BOVIN 297 27 -
GPQRDAATARE GB11_XENLA 297 27 -
GPQRDAQAARE GBQ_XENLA 291 27 -
GPQRDAITARE GBQ3_DROME 291 27 -
GPRKDAIAARE GBQ_HOMAM 291 27 -
GQKKDAQGARE GBQ_PATYE 291 27 -
GPPRDPIAARE Q17386 293 27 -
GPKKDAVQGRE P91955 291 27 -
GPKQDSQAARD O73819 292 27 -
GPKQDVKAARD GB14_BOVIN 293 27 -
GPKQDVKAARD GB14_MOUSE 293 27 -
GPKQDVRAARD O95837 293 27 -
GPKKEASTARE GBQ_LYMST 291 27 -
GPKCDYEAARE GBQ_LOLFO 292 27 -
GPKQDHAAAKQ GBQ1_DROME 291 27 -

Motif 5 width=12
Element Seqn Id St Int Rpt
KMFVDLNPDSDK GB11_HUMAN 311 3 -
KMFVDLNPDSDK GB11_MELGA 311 3 -
KMFVDLNPDSDK GBQ_CANFA 305 3 -
KMFVDLNPDSDK GBQ_MOUSE 305 3 -
KMFVDLNPDSDK GBQ_HUMAN 305 3 -
KMFVDLNPDSDK GB11_MOUSE 311 3 -
KMFVDLNPDSDK Q61939 311 3 -
KMFVDLNPDSDK GB11_BOVIN 311 3 -
KMFVDLNPDSDK GB11_XENLA 311 3 -
KMFVDLNPDSDK GBQ_XENLA 305 3 -
RMFVDLNPDSEK GBQ3_DROME 305 3 -
RMFVELNPDPEK GBQ_HOMAM 305 3 -
RMFVDLNPDPDK GBQ_PATYE 305 3 -
KMFVDLNPDADK Q17386 307 3 -
KMFVDLNPDSEK P91955 305 3 -
KLYQDQNPDKEK O73819 306 3 -
KLYQDQNPDKEK GB14_BOVIN 307 3 -
KLYQDQNPDKEK GB14_MOUSE 307 3 -
KLYQDQNPDKEK O95837 307 3 -
KMFVELNPDPDK GBQ_LYMST 305 3 -
DSYMDLNEDKEK GBQ_LOLFO 306 3 -
KKYLACNPDPER GBQ1_DROME 305 3 -