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PR00430

Identifier
INTERLEUKIN3  [View Relations]  [View Alignment]  
Accession
PR00430
No. of Motifs
3
Creation Date
27-NOV-1995  (UPDATE 10-JUN-1999)
Title
Interleukin-3 signature
Database References

INTERPRO; IPR002183
PDB; 1BGC
SCOP; 1BGC
Literature References
1. DORSSERS, L., BURGER, H., BOT, F., DELWEL, R., GEURTS VAN KESSEL, A.H.,
LOWENBERG, B. AND WAGEMAKER, G.
Characterization of a human multilineage-colony-stimulating factor cDNA
clone identified by a conserved noncoding sequence in mouse interleukin-3.
GENE 55 115-124 (1987).
 
2. YMER, S., TUCKER, W.Q., SANDERSON, C.J., HALPEL, A.J., CAMPBELL, H.D.
AND YOUNG, I.G.
Constitutive synthesis of interleukin-3 by leukaemia cell line WEHI-3B is
due to retroviral insertion near the gene.
NATURE 317 255-258 (1985).

Documentation
Interleukin-3 (IL3) is a cytokine that regulates blood-cell production by 
controlling the production, differentiation and function of granulocytes
and macrophages [1,2]. The protein, which exists in vivo as a monomer, is
produced in activated T-cells and mast cells [1,2], and is activated by the
cleavage of an N-terminal signal sequence [2].
 
IL3 is produced by T-lymphocytes and T-lymphomas only after stimulation
with antigens, mitogens, or chemical activators such as phorbol esters.
However, IL3 is constitutively expressed in the myelomonocytic leukaemia
cell line WEHI-3B [2]. It is thought that the genetic change of the cell
line to constitutive production of IL3 is the key event in development of
this leukaemia [2].
 
INTERLEUKIN3 is a 3-element fingerprint that provides a signature for
the interleukin-3 cytokines. The fingerprint was derived from an initial
alignment of 5 sequences: the motifs were drawn from conserved regions
spanning virtually the full alignment length. Two iterations on OWL26.3
were required to reach convergence, at which point a true set comprising
12 sequences was identified.
 
An update on SPTR37_9f identified a true set of 11 sequences.
Summary Information
11 codes involving  3 elements
0 codes involving 2 elements
Composite Feature Index
3111111
2000
123
True Positives
IL3_BOVIN     IL3_CALJA     IL3_HUMAN     IL3_HYLLA     
IL3_MACMU IL3_MOUSE IL3_PANTR IL3_RAT
IL3_SAGOE IL3_SHEEP P97688
Sequence Titles
IL3_BOVIN   INTERLEUKIN-3 PRECURSOR (IL-3) (MULTIPOTENTIAL COLONY-STIMULATING FACTOR) (HEMATOPOIETIC GROWTH FACTOR) (P-CELL STIMULATING FACTOR) (MAST-CELL GROWTH FACTOR) (MCGF) - BOS TAURUS (BOVINE). 
IL3_CALJA INTERLEUKIN-3 PRECURSOR (IL-3) (MULTIPOTENTIAL COLONY-STIMULATING FACTOR) (HEMATOPOIETIC GROWTH FACTOR) (P-CELL STIMULATING FACTOR) (MAST-CELL GROWTH FACTOR) (MCGF) - CALLITHRIX JACCHUS (COMMON MARMOSET).
IL3_HUMAN INTERLEUKIN-3 PRECURSOR (IL-3) (MULTIPOTENTIAL COLONY-STIMULATING FACTOR) (HEMATOPOIETIC GROWTH FACTOR) (P-CELL STIMULATING FACTOR) (MAST-CELL GROWTH FACTOR) (MCGF) - HOMO SAPIENS (HUMAN).
IL3_HYLLA INTERLEUKIN-3 PRECURSOR (IL-3) (MULTIPOTENTIAL COLONY-STIMULATING FACTOR) (HEMATOPOIETIC GROWTH FACTOR) (P-CELL STIMULATING FACTOR) (MAST-CELL GROWTH FACTOR) (MCGF) - HYLOBATES LAR (COMMON GIBBON).
IL3_MACMU INTERLEUKIN-3 PRECURSOR (IL-3) (MULTIPOTENTIAL COLONY-STIMULATING FACTOR) (HEMATOPOIETIC GROWTH FACTOR) (P-CELL STIMULATING FACTOR) (MAST-CELL GROWTH FACTOR) (MCGF) - MACACA MULATTA (RHESUS MACAQUE).
IL3_MOUSE INTERLEUKIN-3 PRECURSOR (IL-3) (MULTIPOTENTIAL COLONY-STIMULATING FACTOR) (HEMATOPOIETIC GROWTH FACTOR) (P-CELL STIMULATING FACTOR) (MAST-CELL GROWTH FACTOR) (MCGF) - MUS MUSCULUS (MOUSE).
IL3_PANTR INTERLEUKIN-3 PRECURSOR (IL-3) (MULTIPOTENTIAL COLONY-STIMULATING FACTOR) (HEMATOPOIETIC GROWTH FACTOR) (P-CELL STIMULATING FACTOR) (MAST-CELL GROWTH FACTOR) (MCGF) - PAN TROGLODYTES (CHIMPANZEE).
IL3_RAT INTERLEUKIN-3 PRECURSOR (IL-3) (MULTIPOTENTIAL COLONY-STIMULATING FACTOR) (HEMATOPOIETIC GROWTH FACTOR) (P-CELL STIMULATING FACTOR) (MAST-CELL GROWTH FACTOR) (MCGF) - RATTUS NORVEGICUS (RAT).
IL3_SAGOE INTERLEUKIN-3 PRECURSOR (IL-3) (MULTIPOTENTIAL COLONY-STIMULATING FACTOR) (HEMATOPOIETIC GROWTH FACTOR) (P-CELL STIMULATING FACTOR) (MAST-CELL GROWTH FACTOR) (MCGF) - SAGUINUS OEDIPUS (COTTON-TOP TAMARIN).
IL3_SHEEP INTERLEUKIN-3 PRECURSOR (IL-3) (MULTIPOTENTIAL COLONY-STIMULATING FACTOR) (HEMATOPOIETIC GROWTH FACTOR) (P-CELL STIMULATING FACTOR) (MAST-CELL GROWTH FACTOR) (MCGF) - OVIS ARIES (SHEEP).
P97688 INTERLEUKIN-3 BETA - RATTUS NORVEGICUS (RAT).
Scan History
OWL26_3    2  100  NSINGLE    
SPTR37_9f 2 13 NSINGLE
Initial Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
CSNMIDEIITHL IL3_HYLLA 35 35 -
CSNMIDEIITHL IL3_HUMAN 35 35 -
CSNMIDEIITHL IL3_MACMU 35 35 -
YSSLMEEIMDDL IL3_SHEEP 33 33 -
CSSIVKEIIGKL IL3_MOUSE 43 43 -

Motif 2 width=19
Element Seqn Id St Int Rpt
EDQDILMENNLRRPNLEAF IL3_HYLLA 62 15 -
EDQDILMENNLRRPNLEAF IL3_HUMAN 62 15 -
EDQTILVEKNLRRSNLEAF IL3_MACMU 62 15 -
DEKNILANKSLLQANLKAF IL3_SHEEP 58 13 -
DEGPSLRNKSFRRVNLSKF IL3_MOUSE 63 8 -

Motif 3 width=13
Element Seqn Id St Int Rpt
EFRRKLKFYLKTL IL3_HYLLA 125 44 -
EFRRKLTFYLKTL IL3_HUMAN 125 44 -
DFRRKLKFYLKTL IL3_MACMU 125 44 -
DFRMKLEEYLATI IL3_SHEEP 120 43 -
DFRKKLRFYMVHL IL3_MOUSE 126 44 -
Final Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
CSNMIDEIITHL IL3_HYLLA 35 35 -
CSNMIDEIITHL IL3_HUMAN 35 35 -
CSNMIDEIITHL IL3_PANTR 35 35 -
CSNMIDEIITHL IL3_MACMU 35 35 -
CSNLREEIVTLL IL3_SAGOE 34 34 -
CSNLREEIVTLL IL3_CALJA 34 34 -
YSSLMEEIMDDL IL3_SHEEP 33 33 -
CSSIVKEIIGKL IL3_MOUSE 43 43 -
YSMLMKEIMDDL IL3_BOVIN 33 33 -
CRTIALEILVKL IL3_RAT 43 43 -
CRTIALEILVKL P97688 43 43 -

Motif 2 width=19
Element Seqn Id St Int Rpt
EDQDILMENNLRRPNLEAF IL3_HYLLA 62 15 -
EDQDILMENNLRRPNLEAF IL3_HUMAN 62 15 -
EDQDILMENNLRRPNLEAF IL3_PANTR 62 15 -
EDQTILVEKNLRRSNLEAF IL3_MACMU 62 15 -
EDQRILMKPNLRRPNLEAF IL3_SAGOE 61 15 -
EDQRILMKPNLRKPNLEAF IL3_CALJA 61 15 -
DEKNILANKSLLQANLKAF IL3_SHEEP 58 13 -
DEGPSLRNKSFRRVNLSKF IL3_MOUSE 63 8 -
DEKNFLTKESLLQANLKVF IL3_BOVIN 58 13 -
DDKANLRNSTLRRVNLDEF IL3_RAT 63 8 -
DDKANLRNSTLRRVNLDEF P97688 66 11 -

Motif 3 width=13
Element Seqn Id St Int Rpt
EFRRKLKFYLKTL IL3_HYLLA 125 44 -
EFRRKLTFYLKTL IL3_HUMAN 125 44 -
EFRRKLTFYLKTL IL3_PANTR 125 44 -
DFRRKLKFYLKTL IL3_MACMU 125 44 -
DFRMKLKFYLKTL IL3_SAGOE 124 44 -
DFQMKLKFYLKTL IL3_CALJA 124 44 -
DFRMKLEEYLATI IL3_SHEEP 120 43 -
DFRKKLRFYMVHL IL3_MOUSE 126 44 -
DFRMKLEEYLVII IL3_BOVIN 120 43 -
DFKKKLRFYVIHL IL3_RAT 126 44 -
DFKKKLRFYVIHL P97688 129 44 -