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PR00429

Identifier
THROMBOXANER  [View Relations]  [View Alignment]  
Accession
PR00429
No. of Motifs
7
Creation Date
20-NOV-1995  (UPDATE 10-JUN-1999)
Title
Thromboxane receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR01788 PROSTANOIDR
INTERPRO; IPR001105
GCRDB; GCR_0238
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL,
Prostanoids.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, pp239-251.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Prostanoids (prostaglandins (PG) and thromboxanes (TX)) mediate a wide
variety of actions and play important physiological roles in the cardio-
vascular and immune systems, and in pain sensation in peripheral systems
[6]. PGI2 and TXA2 have opposing actions, involving regulation of the
interaction of platelets with the vascular endothelium. To date, evidence
for at least 5 classes of prostanoid receptor has been obtained. However,
identification of subtypes and their distribution is hampered by expression
of more than one receptor within a tissue, coupled with poor selectivity of
available agonists and antagonists. Moreover, many endogenous prostanoids
undergo rapid metabolism, especially TXA2 [6]. 
 
THROMBOXANER is a 7-element fingerprint that provides a signature for the
thromboxane receptors. The fingerprint was derived from an initial
alignment of 4 sequences: the motifs were drawn from conserved sections
either within loop or TM regions, focusing on those areas of the alignment
that characterise the thromboxane receptors but distinguish them from the
rest of the rhodopsin-like superfamily - motif 1 lies in the N-terminus;
motifs 2, 3 and 5 were drawn from TM domains 2, 3 and 5; motifs 4 and 6
span the second external loop and third cytoplasmic loop res.; and motif 7
lies at the C-terminus. A single iteration on OWL26.2 was required to reach
convergence, no further sequences being identified beyond the starting set.
Several partial matches were also found, all of which are closely related
prostaglandin receptors matching motifs 2,3 and 4.
 
An update on SPTR37_9f identified a true set of 6 sequences, and 8
partial matches.
Summary Information
   6 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
8 codes involving 2 elements
Composite Feature Index
76666666
60000000
50000000
40000000
30000000
20583000
1234567
True Positives
O75228        TA2R_BOVIN    TA2R_CERAE    TA2R_HUMAN    
TA2R_MOUSE TA2R_RAT
True Positive Partials
Codes involving 2 elements
PE21_HUMAN PE21_MOUSE PE21_RAT PF2R_BOVIN
PF2R_HUMAN PF2R_MOUSE PF2R_RAT PF2R_SHEEP
Sequence Titles
O75228      TA2R_HUMAN, BETA ISOFORM - Homo sapiens (Human). 
TA2R_BOVIN Thromboxane A2 receptor (TXA2-R) (Prostanoid TP receptor) - Bos taurus (Bovine).
TA2R_CERAE Thromboxane A2 receptor (TXA2-R) (Prostanoid TP receptor) - Cercopithecus aethiops (Green monkey) (Grivet).
TA2R_HUMAN Thromboxane A2 receptor (TXA2-R) (Prostanoid TP receptor) - Homo sapiens (Human).
TA2R_MOUSE Thromboxane A2 receptor (TXA2-R) (Prostanoid TP receptor) - Mus musculus (Mouse).
TA2R_RAT Thromboxane A2 receptor (TXA2-R) (Prostanoid TP receptor) (TXR2) - Rattus norvegicus (Rat).

PE21_HUMAN Prostaglandin E2 receptor, EP1 subtype (Prostanoid EP1 receptor) (PGE receptor, EP1 subtype) - Homo sapiens (Human).
PE21_MOUSE Prostaglandin E2 receptor, EP1 subtype (Prostanoid EP1 receptor) (PGE receptor, EP1 subtype) - Mus musculus (Mouse).
PE21_RAT Prostaglandin E2 receptor, EP1 subtype (Prostanoid EP1 receptor) (PGE receptor, EP1 subtype) - Rattus norvegicus (Rat).
PF2R_BOVIN Prostaglandin F2-alpha receptor (Prostanoid FP receptor) (PGF receptor) (PGF2 alpha receptor) - Bos taurus (Bovine).
PF2R_HUMAN Prostaglandin F2-alpha receptor (Prostanoid FP receptor) (PGF receptor) (PGF2 alpha receptor) - Homo sapiens (Human).
PF2R_MOUSE Prostaglandin F2-alpha receptor (Prostanoid FP receptor) (PGF receptor) (PGF2 alpha receptor) - Mus musculus (Mouse).
PF2R_RAT Prostaglandin F2-alpha receptor (Prostanoid FP receptor) (PGF receptor) (PGF2 alpha receptor) - Rattus norvegicus (Rat).
PF2R_SHEEP Prostaglandin F2-alpha receptor (Prostanoid FP receptor) (PGF receptor) (PGF2 alpha receptor) - Ovis aries (Sheep).
Scan History
OWL26_2    1  50   NSINGLE    
SPTR37_9f 2 77 NSINGLE
Initial Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
FRPVNITLQERRAIASPWF TA2R_MOUSE 12 12 -
FRPVNITLQERRAIASPWF TA2R_RAT 12 12 -
FRPTNITLEERRLIASPWF A53959 12 12 -
FRPTNITLEERRLIASPWF TA2R_HUMAN 12 12 -

Motif 2 width=14
Element Seqn Id St Int Rpt
GLVLTDFLGLLVTG TA2R_RAT 68 37 -
GLVLTDFLGLLVTG TA2R_MOUSE 68 37 -
GLVLTDFLGLLVTG A53959 69 38 -
GLVLTDFLGLLVTG TA2R_HUMAN 69 38 -

Motif 3 width=16
Element Seqn Id St Int Rpt
MGVAMVFFGLCPLLLG TA2R_MOUSE 107 25 -
MGVVMIFFGLSPLLLG A53959 108 25 -
MGVVMIFFGLSPLLLG TA2R_HUMAN 108 25 -
MGAAMVFFGLCPLLLG TA2R_RAT 107 25 -

Motif 4 width=16
Element Seqn Id St Int Rpt
VGRYTVQYPGSWCFLT TA2R_HUMAN 171 47 -
LGRYSVQYPGSWCFLT TA2R_RAT 169 46 -
VGRYTVQYPGSWCFLT A53959 171 47 -
LGRYSVQYPGSWCFLT TA2R_MOUSE 169 46 -

Motif 5 width=18
Element Seqn Id St Int Rpt
GDVAFGLLFSMLGGLSVG A53959 192 5 -
GDVAFGLLFSMLGGLSVG TA2R_HUMAN 192 5 -
GDVVFGLIFALLGSASVG TA2R_MOUSE 190 5 -
GDVAFGLMFALLGSVSVG TA2R_RAT 190 5 -

Motif 6 width=12
Element Seqn Id St Int Rpt
ATQRPRDCEVEM TA2R_RAT 229 21 -
AQQRPRDSEVEM A53959 232 22 -
AQQRPRDSEVEM TA2R_HUMAN 232 22 -
ATQRPRDCEVEM TA2R_MOUSE 229 21 -

Motif 7 width=22
Element Seqn Id St Int Rpt
FRRAVLRRLQPRLSTRPRSLSL TA2R_HUMAN 311 67 -
FRRSVLRRLHPRFTSQLQAVSL TA2R_RAT 308 67 -
FRRSVLRRLHPRFSSQLQAVSL TA2R_MOUSE 308 67 -
FRRAVLRRLQPRLSTRPRRVSL A53959 311 67 -
Final Motifs
Motif 1  width=19
Element Seqn Id St Int Rpt
FRPTNITLEERRLIASPWF TA2R_HUMAN 12 12 -
FRPTNITLEERRLIASPWF TA2R_CERAE 12 12 -
FRPTNITLEERRLIASPWF O75228 12 12 -
FRPMNITLEERRLIASPWF TA2R_BOVIN 12 12 -
FRPVNITLQERRAIASPWF TA2R_MOUSE 12 12 -
FRPVNITLQERRAIASPWF TA2R_RAT 12 12 -

Motif 2 width=14
Element Seqn Id St Int Rpt
GLVLTDFLGLLVTG TA2R_HUMAN 69 38 -
GLVLTDFLGLLVTG TA2R_CERAE 69 38 -
GLVLTDFLGLLVTG O75228 69 38 -
GLVLTDFMGLLVTG TA2R_BOVIN 69 38 -
GLVLTDFLGLLVTG TA2R_MOUSE 68 37 -
GLVLTDFLGLLVTG TA2R_RAT 68 37 -

Motif 3 width=16
Element Seqn Id St Int Rpt
MGVVMIFFGLSPLLLG TA2R_HUMAN 108 25 -
MGVVMIFFGLSPLLLG TA2R_CERAE 108 25 -
MGVVMIFFGLSPLLLG O75228 108 25 -
MGVIMVFFGLCPLLLG TA2R_BOVIN 108 25 -
MGVAMVFFGLCPLLLG TA2R_MOUSE 107 25 -
MGAAMVFFGLCPLLLG TA2R_RAT 107 25 -

Motif 4 width=16
Element Seqn Id St Int Rpt
VGRYTVQYPGSWCFLT TA2R_HUMAN 171 47 -
LGRYTVQYPGSWCFLT TA2R_CERAE 171 47 -
VGRYTVQYPGSWCFLT O75228 171 47 -
VGHYTVQYPGSWCFLT TA2R_BOVIN 171 47 -
LGRYSVQYPGSWCFLT TA2R_MOUSE 169 46 -
LGRYSVQYPGSWCFLT TA2R_RAT 169 46 -

Motif 5 width=18
Element Seqn Id St Int Rpt
GDVAFGLLFSMLGGLSVG TA2R_HUMAN 192 5 -
GDVAFGLLFSMLGGLSVG TA2R_CERAE 192 5 -
GDVAFGLLFSMLGGLSVG O75228 192 5 -
GDVAFGLLFALLGSISVG TA2R_BOVIN 192 5 -
GDVVFGLIFALLGSASVG TA2R_MOUSE 190 5 -
GDVAFGLMFALLGSVSVG TA2R_RAT 190 5 -

Motif 6 width=12
Element Seqn Id St Int Rpt
AQQRPRDSEVEM TA2R_HUMAN 232 22 -
AQQRPRDSEVEM TA2R_CERAE 232 22 -
AQQRPRDSEVEM O75228 232 22 -
AQQRPRDCEVEM TA2R_BOVIN 232 22 -
ATQRPRDCEVEM TA2R_MOUSE 229 21 -
ATQRPRDCEVEM TA2R_RAT 229 21 -

Motif 7 width=22
Element Seqn Id St Int Rpt
FRRAVLRRLQPRLSTRPRSLSL TA2R_HUMAN 311 67 -
FRRAVLRRLQPRLSTRPRSLSL TA2R_CERAE 311 67 -
FRRAVLRRLQPRLSTRPRRSLT O75228 311 67 -
FRRAVIQRFYPRLSTRSRSLSL TA2R_BOVIN 311 67 -
FRRSVLRRLHPRFSSQLQAVSL TA2R_MOUSE 308 67 -
FRRSVLRRLHPRFTSQLQAVSL TA2R_RAT 308 67 -