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PR00426

Identifier
C5ANPHYLTXNR  [View Relations]  [View Alignment]  
Accession
PR00426
No. of Motifs
8
Creation Date
16-DEC-1995  (UPDATE 06-JUN-1999)
Title
C5A-anaphylatoxin receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR01104 ANPHYLATOXNR
INTERPRO; IPR001274
GCRDB; GCR_0464; GCR_0618
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL,
C5a anaphylatoxin.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, pp71-73.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
The accumulation of phagocytic cells at the site of injury or infection is
regulated by substances that stimulate chemotaxis, granule secretion,
superoxide generation and upregulation of cell surface adhesion molecules
in cells of the immune system [6]. The chemoattractant substances include
C5a, N-formylmethionyl-containing peptides, interleukin 8, leukotriene B4
and platelet activating factor, many of which participate in anaphylactoid
and septic shock [6]. The C5a receptor is found on cells of the immune
system (e.g., neutrophils, macrophages, mast cells and related cell lines),
and is also present in smooth muscle. The amino acid sequence of the
receptor contains several N-terminal acidic residues, which may be
involved in binding the basic C5a peptide.
 
C5ANPHYLTXNR is an 8-element fingerprint that provides a signature for the
C5a-anaphylatoxin receptors. The fingerprint was derived from an initial
alignment of 4 sequences: the motifs were drawn from conserved sections
within either loop or TM regions, focusing on those areas of the alignment
that characterise the C5a receptors but distinguish them from the rest of
the rhodopsin-like superfamily - motif 1 lies at the N-terminus of TM
domain 1; motif 2 spans the first cytoplasmic loop; motif 3 lies at the
C-terminus of TM domain 2; motif 4 lies at the N-terminus of TM domain 3;
motif 5 spans the second cytoplasmic loop and the N-terminus of TM domain 4;
motif 6 spans the third cytoplasmic loop; and motifs 7 and 8 lie at the
C-terminus. A single iteration on OWL26.3 was required to reach convergence,
no further sequences being identified beyond the starting set.
 
An update on SPTR37_9f identified a true set of 5 sequences, and 4
partial matches.
Summary Information
   5 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
2 codes involving 3 elements
2 codes involving 2 elements
Composite Feature Index
855555555
700000000
600000000
500000000
400000000
302022000
200022000
12345678
True Positives
C5AR_CANFA    C5AR_HUMAN    C5AR_MOUSE    C5AR_RAT      
O70129
True Positive Partials
Codes involving 3 elements
C3AR_HUMAN O43771
Codes involving 2 elements
C3AR_MOUSE O55197
Sequence Titles
C5AR_CANFA  C5a anaphylatoxin chemotactic receptor (C5a-R) (C5aR) (CD88 antigen) - Canis familiaris (Dog). 
C5AR_HUMAN C5a anaphylatoxin chemotactic receptor (C5a-R) (C5aR) (CD88 antigen) - Homo sapiens (Human).
C5AR_MOUSE C5a anaphylatoxin chemotactic receptor (C5a-R) (C5aR) (CD88 antigen) - Mus musculus (Mouse).
C5AR_RAT C5a anaphylatoxin chemotactic receptor (C5a-R) (C5aR) (CD88 antigen) - Rattus norvegicus (Rat).
O70129 ANAPHYLATOXIN C5A RECEPTOR - CAVIA PORCELLUS (GUINEA PIG).

C3AR_HUMAN C3a anaphylatoxin chemotactic receptor (C3a-R) (C3AR) - Homo sapiens (Human).
O43771 C3A ANAPHYLATOXIN RECEPTOR - HOMO SAPIENS (HUMAN).

C3AR_MOUSE C3a anaphylatoxin chemotactic receptor (C3a-R) (C3AR) (Complement component 3a receptor 1) - Mus musculus (Mouse).
O55197 ANAPHYLATOXIN C3A RECEPTOR - RATTUS NORVEGICUS (RAT).
Scan History
OWL26_3    1  50   NSINGLE    
SPTR37_9f 2 120 NSINGLE
Initial Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
PDILALVIFAVVF C5AR_HUMAN 36 36 -
PDMIALVIFVMVF C5AR_CANFA 37 37 -
GDVAALIIYSVVF A46525 36 36 -
GDVAALIIYSVVF C5AR_MOUSE 32 32 -

Motif 2 width=14
Element Seqn Id St Int Rpt
VTAFEPDGPSNAIW C5AR_MOUSE 57 12 -
VTGFEVRRTINAIW C5AR_CANFA 62 12 -
VTAFEPDGPSNAIW A46525 61 12 -
VTAFEAKRTINAIW C5AR_HUMAN 61 12 -

Motif 3 width=11
Element Seqn Id St Int Rpt
SCLAMPVLFTT C5AR_MOUSE 81 10 -
SCLAMPVLFTT A46525 85 10 -
SCLALPILFTS C5AR_HUMAN 85 10 -
SCLALPILFSS C5AR_CANFA 86 10 -

Motif 4 width=12
Element Seqn Id St Int Rpt
LPSLILLNMYAS C5AR_MOUSE 108 16 -
LPSLILLNMYAS A46525 112 16 -
LPSLILLNMYAS C5AR_HUMAN 112 16 -
LPSLILLNMYAS C5AR_CANFA 113 16 -

Motif 5 width=16
Element Seqn Id St Int Rpt
IWCQNFRGAGLAWIAC C5AR_HUMAN 142 18 -
IWCQKVRGTGLAWMAC A46525 142 18 -
IWCQKVRGTGLAWMAC C5AR_MOUSE 138 18 -
IWCQNYRGPQLAWAAC C5AR_CANFA 143 18 -

Motif 6 width=15
Element Seqn Id St Int Rpt
RTWSRKATRSTKTLK A46525 229 71 -
RTWSRRATRSTKTLK C5AR_HUMAN 228 70 -
RTWSRKATRSTKTLK C5AR_CANFA 230 71 -
RTWSRKATRSTKTLK C5AR_MOUSE 225 71 -

Motif 7 width=14
Element Seqn Id St Int Rpt
RNALSEDSVGRDSK C5AR_MOUSE 317 77 -
RNALSEDSVGRDSK A46525 321 77 -
RNVLTEESVVRESK C5AR_HUMAN 320 77 -
RQVLAEESVGRDSK C5AR_CANFA 322 77 -

Motif 8 width=16
Element Seqn Id St Int Rpt
KSFTRSTVDTMAQKTQ C5AR_HUMAN 333 -1 -
KTFTPSTDDTSPRKSQ C5AR_MOUSE 330 -1 -
KSITLSTVDTPAQKSQ C5AR_CANFA 335 -1 -
KTFTPSTDDTSTRKSQ A46525 334 -1 -
Final Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
PDILALVIFAVVF C5AR_HUMAN 36 36 -
GDIAALIIYLAVF C5AR_RAT 37 37 -
PDMIALVIFVMVF C5AR_CANFA 37 37 -
GDVAALIIYSVVF C5AR_MOUSE 32 32 -
GDLVAVVIMVVVF O70129 31 31 -

Motif 2 width=14
Element Seqn Id St Int Rpt
VTAFEAKRTINAIW C5AR_HUMAN 61 12 -
VTAFEAKRTVNAIW C5AR_RAT 62 12 -
VTGFEVRRTINAIW C5AR_CANFA 62 12 -
VTAFEPDGPSNAIW C5AR_MOUSE 57 12 -
VTACEARRHINAIW O70129 56 12 -

Motif 3 width=11
Element Seqn Id St Int Rpt
SCLALPILFTS C5AR_HUMAN 85 10 -
SCLALPILFTS C5AR_RAT 86 10 -
SCLALPILFSS C5AR_CANFA 86 10 -
SCLAMPVLFTT C5AR_MOUSE 81 10 -
SCLALPILLVS O70129 80 10 -

Motif 4 width=12
Element Seqn Id St Int Rpt
LPSLILLNMYAS C5AR_HUMAN 112 16 -
LPSLILLNMYSS C5AR_RAT 113 16 -
LPSLILLNMYAS C5AR_CANFA 113 16 -
LPSLILLNMYAS C5AR_MOUSE 108 16 -
LPSLILLNMYTS O70129 107 16 -

Motif 5 width=16
Element Seqn Id St Int Rpt
IWCQNFRGAGLAWIAC C5AR_HUMAN 142 18 -
IWCQKFRRPGLAWMAC C5AR_RAT 143 18 -
IWCQNYRGPQLAWAAC C5AR_CANFA 143 18 -
IWCQKVRGTGLAWMAC C5AR_MOUSE 138 18 -
IWCQRFRGGCLAWTAC O70129 137 18 -

Motif 6 width=15
Element Seqn Id St Int Rpt
RTWSRRATRSTKTLK C5AR_HUMAN 228 70 -
RTWSRKATRSTKTLK C5AR_RAT 230 71 -
RTWSRKATRSTKTLK C5AR_CANFA 230 71 -
RTWSRKATRSTKTLK C5AR_MOUSE 225 71 -
RTWSRKATRSAKTVK O70129 223 70 -

Motif 7 width=14
Element Seqn Id St Int Rpt
RNVLTEESVVRESK C5AR_HUMAN 320 77 -
RNVLSEDSLGRDSK C5AR_RAT 322 77 -
RQVLAEESVGRDSK C5AR_CANFA 322 77 -
RNALSEDSVGRDSK C5AR_MOUSE 317 77 -
RNVLTEESLDKRHQ O70129 315 77 -

Motif 8 width=16
Element Seqn Id St Int Rpt
KSFTRSTVDTMAQKTQ C5AR_HUMAN 333 -1 -
KSFTRSTMDTSTQKSQ C5AR_RAT 335 -1 -
KSITLSTVDTPAQKSQ C5AR_CANFA 335 -1 -
KTFTPSTDDTSPRKSQ C5AR_MOUSE 330 -1 -
QSFARSTVDTMPQKSE O70129 328 -1 -