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PR00402

Identifier
TECBTKDOMAIN  [View Relations]  [View Alignment]  
Accession
PR00402
No. of Motifs
3
Creation Date
11-DEC-1995  (UPDATE 19-JUN-1999)
Title
Tec/Btk domain signature
Database References

PFAM; PF00779 BTK
INTERPRO; IPR001562
Literature References
1. VIHINEN, M., NILSSON, L. AND SMITH, C.I.E.
Tec homology (TH) adjacent to the PH domain.
FEBS LETT. 350 263-265 (1994).

Documentation
Kinases are generally multi-domain, multi-functional proteins. Protein
tyrosine kinases (PTKs), serine/threonine kinases, and other signal
transduction proteins, possess a region of unknown function related to
pleckstrin, designated the PH domain. A point mutation affecting a
conserved Arg in the PH domain of the cytoplasmic PTK Btk causes the human
disease X-linked agammaglobulinemia and X-linked immunodeficiency in mice.
 
Btk forms a family with 2 other PTKs, Isk/Tsk and Tec, in which the PH
domain is followed by an SH3 domain. The conserved sequence between the
2 regions has tentatively been designated the TH (Tec homology) domain.
The N-terminal 27 residues of the TH domain are highly conserved (the
Btk motif), and are followed by a proline-rich (PRR) region: the Btk motif
contains a conserved His and 3 Cys residues that are involved in zinc
fingers (although these differ from known zinc finger topologies), while
PRRs are commonly involved in protein-protein interactions. The Tec
extension to the PH domain may be of functional importance in various
signalling pathways in different species [1]. A complete TH domain,
containing both the Btk and PRR regions, has not been found outside the
Btk family, and may be a hallmark of these cytoplasmic PTKs.
 
TECBTKDOMAIN is a 3-element fingerprint that provides a signature for the
Tec Btk motif. The fingerprint was derived from an initial alignment of 7
sequences: the motifs completely span the conserved Btk region - motifs 2
and 3 contain the His and Cys residues that participate in zinc fingers.
Two iterations on OWL26.3 were required to reach convergence, at which
point a true set comprising 18 sequences was identified. A single partial
match was also found, HSINSP4BP, an Ins P4-binding protein that fails to
make a significant match with motif 3. 
 
An update on SPTR37_9f identified a true set of 19 sequences.
Summary Information
19 codes involving  3 elements
0 codes involving 2 elements
Composite Feature Index
3191919
2000
123
True Positives
BMX_HUMAN     BTK_HUMAN     BTK_MOUSE     GAP1_DROME    
ITK_HUMAN ITK_MOUSE O42200 O42455
O43374 O60564 P97504 Q14644
Q15283 Q28013 Q60790 Q63713
Q92594 TEC_HUMAN TEC_MOUSE
Sequence Titles
BMX_HUMAN   CYTOPLASMIC TYROSINE-PROTEIN KINASE BMX (EC 2.7.1.112) (BONE MARROW KINASE, X-LINKED) - HOMO SAPIENS (HUMAN). 
BTK_HUMAN TYROSINE-PROTEIN KINASE BTK (EC 2.7.1.112) (BRUTON'S TYROSINE KINASE) (AGAMMAGLOBULINAEMIA TYROSINE KINASE) (ATK) (B CELL PROGENITOR KINASE) (BPK) - HOMO SAPIENS (HUMAN).
BTK_MOUSE TYROSINE-PROTEIN KINASE BTK (EC 2.7.1.112) (BRUTON'S TYROSINE KINASE) (AGAMMAGLOBULINAEMIA TYROSINE KINASE) (ATK) (B CELL PROGENITOR KINASE) (BPK) (KINASE EMB) - MUS MUSCULUS (MOUSE).
GAP1_DROME GTPASE-ACTIVATING PROTEIN 1 - DROSOPHILA MELANOGASTER (FRUIT FLY).
ITK_HUMAN TYROSINE-PROTEIN KINASE ITK/TSK (EC 2.7.1.112) (T-CELL-SPECIFIC KINASE) (TYROSINE-PROTEIN KINASE LYK) (KINASE EMT) - HOMO SAPIENS (HUMAN).
ITK_MOUSE TYROSINE-PROTEIN KINASE ITK/TSK (EC 2.7.1.112) (T-CELL-SPECIFIC KINASE) (IL-2-INDUCIBLE T-CELL KINASE) (KINASE EMT) (KINASE TLK) - MUS MUSCULUS (MOUSE).
O42200 TEC-FAMILY KINASE - BRACHYDANIO RERIO (ZEBRAFISH) (ZEBRA DANIO).
O42455 PROTEIN TYROSINE KINASE - RAJA EGLANTERIA.
O43374 SIMILAR TO GTPASE-ACTIVATING PROTEINS - HOMO SAPIENS (HUMAN).
O60564 ETK/BMX CYTOSOLIC TYROSINE KINASE - HOMO SAPIENS (HUMAN).
P97504 BMX NON-RECEPTOR TYROSINE KINASE (PROTEIN TYROSINE KINASE BMX) - MUS MUSCULUS (MOUSE).
Q14644 INS P4-BINDING PROTEIN - HOMO SAPIENS (HUMAN).
Q15283 RASGTPASE ACTIVATING PROTEIN - HOMO SAPIENS (HUMAN).
Q28013 R-RAS GTPASE ACTIVATING PROTEIN - BOS TAURUS (BOVINE).
Q60790 RAS P21 PROTEIN ACTIVATOR 3 (BRAIN-ENRICHED GTPASE-ACTIVATING PROTEIN GAPIII) - MUS MUSCULUS (MOUSE).
Q63713 RAS GTPASE-ACTIVATING PROTEIN - RATTUS NORVEGICUS (RAT).
Q92594 RAS GTPASE-ACTIVATING PROTEIN (GAP1M) - HOMO SAPIENS (HUMAN).
TEC_HUMAN TYROSINE-PROTEIN KINASE TEC (EC 2.7.1.112) - HOMO SAPIENS (HUMAN).
TEC_MOUSE TYROSINE-PROTEIN KINASE TEC (EC 2.7.1.112) - MUS MUSCULUS (MOUSE).
Scan History
OWL26_3    2  50   NSINGLE    
SPTR37_9f 3 50 NSINGLE
Initial Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
WVKKLKEEIKNNNNIMIKYH KTEC_MOUSE 21 21 -
WVKKLKEEIKNNNNIMIKYH JU0228 102 102 -
WVLTLKEETRNNNSLVSKYH KITK_MOUSE 108 108 -
WVLTLKEETRNNNSLVSKYH JN0472 102 102 -
WIHQLKNVIRYNSDLVQKYH KBTK_HUMAN 124 124 -
WIHQLKNVIRYNSDLVQKYH KBTK_MOUSE 124 124 -
WFDLLHKICLMNSIRMQYFH S27809 853 853 -

Motif 2 width=13
Element Seqn Id St Int Rpt
HPKFWADGSYQCC KTEC_MOUSE 40 -1 -
HPKFWADGSYQCC JU0228 121 -1 -
HPNFWMDGRWRCC KITK_MOUSE 127 -1 -
HPNFWMDGRWRCC JN0472 121 -1 -
HPCFWIDGQYLCC KBTK_HUMAN 143 -1 -
HPCFWIDGQYLCC KBTK_MOUSE 143 -1 -
HPSAFVSGFYSCC S27809 872 -1 -

Motif 3 width=14
Element Seqn Id St Int Rpt
CRQTEKLAPGCEKY KTEC_MOUSE 52 -1 -
CRQTEKLAPGCEKY JU0228 133 -1 -
CSQLEKPAVGCAPY KITK_MOUSE 139 -1 -
CSQLEKPAVGCAPY JN0472 133 -1 -
CSQTAKNAMGCQIL KBTK_HUMAN 155 -1 -
CSQTAKNAMGCQIL KBTK_MOUSE 155 -1 -
CGRSDENSPGCKKV S27809 884 -1 -
Final Motifs
Motif 1  width=20
Element Seqn Id St Int Rpt
WLKALQKEIRGNPHLLIKYH P97504 102 102 -
WLKALQKEIRGNPHLLVKYH O60564 125 125 -
WLKALQKEIRGNPHLLVKYH BMX_HUMAN 102 102 -
WIHQLKNVIRYNSDLVQKYH BTK_HUMAN 124 124 -
WIHQLKNVIRYNSDLVQKYH BTK_MOUSE 124 124 -
WVKKLKEEIKNNNNIMIKYH TEC_HUMAN 102 102 -
WVKKLKEEIKNNNNIMIKYH TEC_MOUSE 102 102 -
WVLALKEETRNNNSLVPKYH ITK_HUMAN 102 102 -
WIDILTKVSQCNQKRLAVYH Q28013 668 668 -
WITELGNRIRFNTELLQKYH O42455 98 98 -
WVLTLKEETRNNNSLVSKYH ITK_MOUSE 108 108 -
WIDILTKVSQCNQKRLTVYH Q14644 668 668 -
WIDVLCRVSRCNQNRLSFYH Q15283 697 697 -
WIDVLCRVSRCNQNRLSFYH Q92594 700 700 -
WIDILTKVSQCNQKRLTVFH Q60790 668 668 -
WIDMLCRVSRCNHNRLSSFH Q63713 695 695 -
WVKALKEAIQNNSLFLKYHP O42200 102 102 -
WLSALRKVSINNTGLLGSYH O43374 687 687 -
WFDLLHKICLMNSIRMQYFH GAP1_DROME 853 853 -

Motif 2 width=13
Element Seqn Id St Int Rpt
HSGFFVDGKFLCC P97504 121 -1 -
HSGFFVDGKFLCC O60564 144 -1 -
HSGFFVDGKFLCC BMX_HUMAN 121 -1 -
HPCFWIDGQYLCC BTK_HUMAN 143 -1 -
HPCFWIDGQYLCC BTK_MOUSE 143 -1 -
HPKFWTDGSYQCC TEC_HUMAN 121 -1 -
HPKFWADGSYQCC TEC_MOUSE 121 -1 -
HPNFWMDGKWRCC ITK_HUMAN 121 -1 -
HPSAYLNGHWLCC Q28013 687 -1 -
HPCFWMEGEWSCC O42455 117 -1 -
HPNFWMDGRWRCC ITK_MOUSE 127 -1 -
HPSAYLSGHWLCC Q14644 687 -1 -
HPSVYLNGNWLCC Q15283 716 -1 -
HPSVYLNGNWLCC Q92594 719 -1 -
HPSAYLNGHWLCC Q60790 687 -1 -
HPSAYLNGNWLCC Q63713 714 -1 -
HPDFWGEGKWRCC O42200 120 -2 -
HPGVFRGDKWSCC O43374 706 -1 -
HPSAFVSGFYSCC GAP1_DROME 872 -1 -

Motif 3 width=14
Element Seqn Id St Int Rpt
CQQSCKAAPGCTLW P97504 133 -1 -
CQQSCKAAPGCTLW O60564 156 -1 -
CQQSCKAAPGCTLW BMX_HUMAN 133 -1 -
CSQTAKNAMGCQIL BTK_HUMAN 155 -1 -
CSQTAKNAMGCQIL BTK_MOUSE 155 -1 -
CRQTEKLAPGCEKY TEC_HUMAN 133 -1 -
CRQTEKLAPGCEKY TEC_MOUSE 133 -1 -
CSQLEKLATGCAQY ITK_HUMAN 133 -1 -
CRASSDTAAGCSPC Q28013 699 -1 -
CQQSAKTAPGCCLV O42455 129 -1 -
CSQLEKPAVGCAPY ITK_MOUSE 139 -1 -
CRAPSDSAPGCSPC Q14644 699 -1 -
CQETGENTLGCKPC Q15283 728 -1 -
CQETGENTLGCKPC Q92594 731 -1 -
CRASSDTAAGCTPC Q60790 699 -1 -
CQETSEGTPGCKPC Q63713 726 -1 -
CQQTEKLATGCSEY O42200 132 -1 -
CHQKEKTGQGCDKT O43374 718 -1 -
CGRSDENSPGCKKV GAP1_DROME 884 -1 -