SPRINT Home UMBER Home Contents Standard Search Advanced Search Relation Search

==SPRINT==> PRINTS View



  selected as


PR00399

Identifier
SYNAPTOTAGMN  [View Relations]  [View Alignment]  
Accession
PR00399
No. of Motifs
4
Creation Date
06-JUL-1995  (UPDATE 28-JUN-1999)
Title
Synaptotagmin signature
Database References

PROSITE; PS00499 C2_DOMAIN
BLOCKS; BL00499
INTERPRO; IPR001565
Literature References
1. SUTTON, R.B., DAVLETOV, B.A., BERGHUIS, A.M., SUDHOF, T.C.
AND SPRANG, S.R.
Structure of the first C2 domain of synaptotagmin I: a novel
Ca2+/phospholipid-binding fold.
CELL 80 929-938 (1995).
 
2. FUJUDA, M., ARUGA, J., NIINOBE, M., AIMOTO, S. AND MIKOSHIBA, K.
Inositol-1,3,4,5-tetrakisphosphate binding to C2B domain of IP4BP/
synaptotagmin II.
J.BIOL.CHEM. 269(46) 29206-29211 (1994).

Documentation
Synaptotagmins are synaptic vesicle membrane proteins found in abundance
in nerve cells and some endocrine cells [1,2]. The amino acid sequence
of synaptotagmin comprises a single transmembrane region with a short
vesicular N-terminus, and a cytoplasmic C-terminal region containing 2
internal repeats similar to the C2 regulatory domain of protein kinase C.
The protein is believed to be important in the docking and fusion of
synaptic vesicles with the plasma membrane, i.e. with neurotransmitter
release [1,2]. 
 
The 2 synaptotagmin C2 domains have been shown to have different functions:
C2A binds phospholipid in a calcium-dependent manner, while C2B binds
inositol polyphosphate and phospholipid irrespective of the presence of
Ca2+ [2]. The structure of C2 domains in synaptotagmin I has been deduced:
the C2 polypeptide forms an 8-stranded beta-sandwich constructed around a 
conserved 4-stranded motif, designated a C2 key [1]. The calcium binding
region is a cup-shaped depression formed by the N- and C-terminal loops
of the C2-key motif, while the site of phospholipid interaction is
thought to be a polybasic sequence on the hairpin loop connecting
strands 3 and 4 [1]. 
 
SYNAPTOTAGMN is a 4-element fingerprint that provides a signature for
synaptotagmins. The fingerprint was derived from an initial alignment of
4 sequences: the motifs were drawn from strands 1, 2, 6 and 7 res.. Two
iterations on OWL26.0 were required to reach convergence, at which point
a true set comprising 20 sequences was identified. Three partial matches
were also found, which are closely related cellutagmin and rabphilins.
 
An update on SPTR37_9f identified a true set of 34 sequences, and 3
partial matches.
Summary Information
  34 codes involving  4 elements
0 codes involving 3 elements
1 codes involving 2 elements
Composite Feature Index
434343434
30000
21001
1234
True Positives
O08835        P70169        P70610        P70611        
P97791 Q14183 Q14184 Q20354
Q25393 Q62746 Q62747 Q62807
RP3A_BOVIN RP3A_RAT SY61_DISOM SY62_DISOM
SY63_DISOM SY65_APLCA SY65_DROME SYT1_BOVIN
SYT1_CAEEL SYT1_CHICK SYT1_HUMAN SYT1_MOUSE
SYT1_RAT SYT2_MOUSE SYT2_RAT SYT3_MOUSE
SYT3_RAT SYT4_MOUSE SYT4_RAT SYT5_HUMAN
SYT5_RAT YPT7_CAEEL
True Positive Partials
Codes involving 2 elements
P91493
Sequence Titles
O08835      SYNAPTOTAGMIN XI - RATTUS NORVEGICUS (RAT).   
P70169 DOC2BETA - MUS MUSCULUS (MOUSE).
P70610 DOC2A - RATTUS NORVEGICUS (RAT).
P70611 DOC2B - RATTUS NORVEGICUS (RAT).
P97791 SYNAPTOTAGMIN 3 - MUS MUSCULUS (MOUSE).
Q14183 DOC2 - HOMO SAPIENS (HUMAN).
Q14184 DOC2 BETA - HOMO SAPIENS (HUMAN).
Q20354 SIMILAR TO SYNAPTOTAGMIN - CAENORHABDITIS ELEGANS.
Q25393 SYNAPTOTAGMIN - LOLIGO PEALEII (LONGFIN SQUID).
Q62746 SYNAPTOTAGMIN VI - RATTUS NORVEGICUS (RAT).
Q62747 SYNAPTOTAGMIN VII - RATTUS NORVEGICUS (RAT).
Q62807 B/K PROTEIN - RATTUS NORVEGICUS (RAT).
RP3A_BOVIN RABPHILIN-3A - BOS TAURUS (BOVINE).
RP3A_RAT RABPHILIN-3A - RATTUS NORVEGICUS (RAT).
SY61_DISOM SYNAPTOTAGMIN A (SYNAPTIC VESICLE PROTEIN O-P65-A) - DISCOPYGE OMMATA (ELECTRIC RAY).
SY62_DISOM SYNAPTOTAGMIN B (SYNAPTIC VESICLE PROTEIN O-P65-B) - DISCOPYGE OMMATA (ELECTRIC RAY).
SY63_DISOM SYNAPTOTAGMIN C (SYNAPTIC VESICLE PROTEIN O-P65-C) - DISCOPYGE OMMATA (ELECTRIC RAY).
SY65_APLCA SYNAPTOTAGMIN (P65) - APLYSIA CALIFORNICA (CALIFORNIA SEA HARE).
SY65_DROME SYNAPTOTAGMIN (P65) - DROSOPHILA MELANOGASTER (FRUIT FLY).
SYT1_BOVIN SYNAPTOTAGMIN I (P65) - BOS TAURUS (BOVINE).
SYT1_CAEEL SYNAPTOTAGMIN I - CAENORHABDITIS ELEGANS.
SYT1_CHICK SYNAPTOTAGMIN I (P65) - GALLUS GALLUS (CHICKEN).
SYT1_HUMAN SYNAPTOTAGMIN I (P65) - HOMO SAPIENS (HUMAN).
SYT1_MOUSE SYNAPTOTAGMIN I (P65) - MUS MUSCULUS (MOUSE).
SYT1_RAT SYNAPTOTAGMIN I (P65) - RATTUS NORVEGICUS (RAT).
SYT2_MOUSE SYNAPTOTAGMIN II - MUS MUSCULUS (MOUSE).
SYT2_RAT SYNAPTOTAGMIN II - RATTUS NORVEGICUS (RAT).
SYT3_MOUSE SYNAPTOTAGMIN III (SYTIII) - MUS MUSCULUS (MOUSE).
SYT3_RAT SYNAPTOTAGMIN III - RATTUS NORVEGICUS (RAT).
SYT4_MOUSE SYNAPTOTAGMIN IV - MUS MUSCULUS (MOUSE).
SYT4_RAT SYNAPTOTAGMIN IV - RATTUS NORVEGICUS (RAT).
SYT5_HUMAN SYNAPTOTAGMIN V - HOMO SAPIENS (HUMAN).
SYT5_RAT SYNAPTOTAGMIN V - RATTUS NORVEGICUS (RAT).
YPT7_CAEEL HYPOTHETICAL 111.7 KD PROTEIN F37A4.7 IN CHROMOSOME III - CAENORHABDITIS ELEGANS.

P91493 SIMILAR TO THE C2 DOMAINS OF SYNAPTOTAGMINS - CAENORHABDITIS ELEGANS.
Scan History
OWL26_0    2  100  NSINGLE    
SPTR37_9f 5 100 NSINGLE
Initial Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
LQYSLDYDFQNNQLLV SYT1_RAT 145 145 -
LQFSLDYDFQANQLTV SYT2_RAT 146 146 -
ISFALRYLYGSDQLVV SYT3_RAT 301 301 -
LLVSLCYQSTTNTLTV SYT4_MOUSE 291 291 -

Motif 2 width=14
Element Seqn Id St Int Rpt
VGIIQAAELPALDM SYT1_RAT 160 -1 -
VGVLQAAELPALDM SYT2_RAT 161 -1 -
VRILQALDLPAKDS SYT3_RAT 316 -1 -
VVVLKARHLPKSDV SYT4_MOUSE 306 -1 -

Motif 3 width=16
Element Seqn Id St Int Rpt
PYSELGGKTLVMAVYD SYT1_RAT 215 41 -
PYQELGGKTLVMAIYD SYT2_RAT 216 41 -
PLAELAQRKLHFSVYD SYT3_RAT 371 41 -
PCESLEEISVEFLVLD SYT4_MOUSE 363 43 -

Motif 4 width=11
Element Seqn Id St Int Rpt
SKHDIIGEFKV SYT1_RAT 235 4 -
SKHDIIGEVKV SYT2_RAT 236 4 -
SRHDLIGQVVL SYT3_RAT 391 4 -
SRNEVIGRLVL SYT4_MOUSE 383 4 -
Final Motifs
Motif 1  width=16
Element Seqn Id St Int Rpt
ICFSLRYVPTAGKLTV SY61_DISOM 282 282 -
ICFSLRYVPTAGKLTV SYT1_MOUSE 276 276 -
ICFSLRYVPTAGKLTV SYT1_HUMAN 277 277 -
ICFSLRYVPTAGKLTV SYT1_BOVIN 277 277 -
ICFSLRYVPTAGKLTV SYT1_CHICK 279 279 -
IQFSLDYDFQANQLTV SY62_DISOM 163 163 -
LQYSLDYDFQNNQLLV SYT1_RAT 145 145 -
LQFSLDYDFQANQLTV SYT2_MOUSE 146 146 -
LQFSLDYDFQANQLTV SYT2_RAT 146 146 -
ICFSLRYVPTAGKLTV SYT1_CAEEL 296 296 -
LQYSLDYDFQTGQLLV SYT5_RAT 112 112 -
LQYSLDYDFQSGQLLV SYT5_HUMAN 112 112 -
LQFSLDYDFQKGELSV SY65_APLCA 155 155 -
LQYSMDYDFQKGELTV Q25393 152 152 -
INFSLRYDYESETLIV Q62746 234 234 -
INFMLRYTYTTEQLVV SY63_DISOM 240 240 -
ISFALRYLYGSDQLVV P97791 300 300 -
ISFALRYLYGSDQLVV SYT3_RAT 301 301 -
ISFALRYLYGSDQLVV SYT3_MOUSE 300 300 -
ILISLKYSSQKQGLLV P70610 270 270 -
ILISLKYSSQKQGLLV P70169 270 270 -
ILISLKYSSQKQGLLV Q14184 270 270 -
LNFKLEYDFNSNSLAV SY65_DROME 196 196 -
IQFSVGYNFQESTLTV Q62747 139 139 -
ILLSLSYSSRRRGLLV Q14183 255 255 -
ILLSLSYSSRRHGLLV P70611 258 258 -
ILVSLMYSTQQGGLIV RP3A_RAT 544 544 -
ILVSLMYSTQQGGLIV RP3A_BOVIN 564 564 -
LFLSLEYNFEKKAFVV SYT4_RAT 157 157 -
LLLSLNYLPSAGRLNV Q62807 325 325 -
LLVSLCYQSTTNTLTV SYT4_MOUSE 291 291 -
LTFSVDYNFPKKALVV O08835 160 160 -
ICFSTRYRPATGTVTL Q20354 108 108 -
INVGLQYNIQQGSLFI YPT7_CAEEL 886 886 -

Motif 2 width=14
Element Seqn Id St Int Rpt
VVILEAKNLKKMDV SY61_DISOM 297 -1 -
VVILEAKNLKKMDV SYT1_MOUSE 291 -1 -
VVILEAKNLKKMDV SYT1_HUMAN 292 -1 -
VVILEAKNLKKMDV SYT1_BOVIN 292 -1 -
VVILEAKNLKKMDV SYT1_CHICK 294 -1 -
VGIIQAAELPALDM SY62_DISOM 178 -1 -
VGIIQAAELPALDM SYT1_RAT 160 -1 -
VGVLQAAELPALDM SYT2_MOUSE 161 -1 -
VGVLQAAELPALDM SYT2_RAT 161 -1 -
VVILEAKNLKKMDV SYT1_CAEEL 311 -1 -
VGILQAEGLAALDL SYT5_RAT 127 -1 -
VGILQAMGLAALDL SYT5_HUMAN 127 -1 -
VNVIQAADLPGMDM SY65_APLCA 170 -1 -
VNVIQAADLPGMDM Q25393 167 -1 -
VRILKAFDLPAKDF Q62746 249 -1 -
VKILKALDLPAKDA SY63_DISOM 255 -1 -
VRILQALDLPAKDS P97791 315 -1 -
VRILQALDLPAKDS SYT3_RAT 316 -1 -
VRILQALDLPAKDS SYT3_MOUSE 315 -1 -
VGIVRCAHLAAMDA P70610 285 -1 -
VGIVRCAHLAAMDA P70169 285 -1 -
VGIVRCAHLAAMDA Q14184 285 -1 -
VTVIQAEELPALDM SY65_DROME 211 -1 -
VNIIKARNLKAMDI Q62747 285 130 -
VGILRCAHLAAMDV Q14183 270 -1 -
VGIVRCAHLAAMDV P70611 273 -1 -
VGIIRCVHLAAMDA RP3A_RAT 559 -1 -
VGIIRCVHLAAMDA RP3A_BOVIN 579 -1 -
VNIKEAQGLPAMDE SYT4_RAT 172 -1 -
VDIIRAKQLLQTDV Q62807 340 -1 -
VVVLKARHLPKSDV SYT4_MOUSE 306 -1 -
VTIQEAHGLPVMDG O08835 175 -1 -
LTIMEARNLKKMDV Q20354 123 -1 -
ININRCVELVGMDS YPT7_CAEEL 901 -1 -

Motif 3 width=16
Element Seqn Id St Int Rpt
PFEQIQKVQVVVTVLD SY61_DISOM 354 43 -
PFEQIQKVQVVVTVLD SYT1_MOUSE 348 43 -
PFEQIQKVQVVVTVLD SYT1_HUMAN 349 43 -
PFEQIQKVQVVVTVLD SYT1_BOVIN 349 43 -
PFEQIQKVQIVVTVLD SYT1_CHICK 351 43 -
PYQELGGKTLMMAVYD SY62_DISOM 233 41 -
PYSELGGKTLVMAVYD SYT1_RAT 215 41 -
PYQELAGKTLVMAIYD SYT2_MOUSE 216 41 -
PYQELGGKTLVMAIYD SYT2_RAT 216 41 -
PFEQIQKVSLMITVMD SYT1_CAEEL 368 43 -
PYVELGGRVLVMAVYD SYT5_RAT 182 41 -
PYVELGGRVLVMAVYD SYT5_HUMAN 182 41 -
PYAEVGSKILTFAVYD SY65_APLCA 225 41 -
PYADITGKTLVFAIYD Q25393 223 42 -
PYEELADRKLHLSVFD Q62746 304 41 -
PFNELQNRKLHFSVYD SY63_DISOM 310 41 -
PLAELAQRKLHFSVYD P97791 370 41 -
PLAELAQRKLHFSVYD SYT3_RAT 371 41 -
PLAELAQRKLHFSVYD SYT3_MOUSE 370 41 -
KHGDLAKKTLEVTVWD P70610 342 43 -
KHGDLAKKTLEVTVWD P70169 342 43 -
KHGDLAKKSLEVTVWD Q14184 342 43 -
PYADAMNKTLVFAIFD SY65_DROME 267 42 -
PTEKLRETTIIITVMD Q62747 342 43 -
ELSTLATKTLEVTVWD Q14183 327 43 -
ELSTLATKTLEVTVWD P70611 330 43 -
KHSDLAKKSLDISVWD RP3A_RAT 616 43 -
KHSDLAKKSLDISVWD RP3A_BOVIN 636 43 -
PYPHIQELSLHFTVLS SYT4_RAT 229 43 -
PQEELENASLVFTVFG Q62807 397 43 -
PCESLEEISVEFLVLD SYT4_MOUSE 363 43 -
PYSQLQDLVLHFLVLS O08835 232 43 -
EPHMIEKVHLIVSVWD Q20354 180 43 -
PFKDLPKKTLQIGVYD YPT7_CAEEL 958 43 -

Motif 4 width=11
Element Seqn Id St Int Rpt
GKNDAIGKVFV SY61_DISOM 374 4 -
GKNDAIGKVFV SYT1_MOUSE 368 4 -
GKNDAIGKVFV SYT1_HUMAN 369 4 -
GKNDAIGKVFV SYT1_BOVIN 369 4 -
GKNDAIGKVFV SYT1_CHICK 371 4 -
SKHDCIGQVTV SY62_DISOM 253 4 -
SKHDIIGEFKV SYT1_RAT 235 4 -
SKHDIIGEVKV SYT2_MOUSE 236 4 -
SKHDIIGEVKV SYT2_RAT 236 4 -
GSNDAIGRCLL SYT1_CAEEL 388 4 -
SRNDAIGEVRV SYT5_RAT 202 4 -
SRNDAIGEVRV SYT5_HUMAN 202 4 -
SKHDQIGQVQV SY65_APLCA 245 4 -
SKHDQIGQVQV Q25393 243 4 -
SRHDMIGEVIL Q62746 324 4 -
SRHDLIGQVVL SY63_DISOM 330 4 -
SRHDLIGQVVL P97791 390 4 -
SRHDLIGQVVL SYT3_RAT 391 4 -
SRHDLIGQVVL SYT3_MOUSE 390 4 -
KSNDFIGGVVL P70610 362 4 -
KSNDFIGGVVL P70169 362 4 -
KSNDFIGGVVL Q14184 362 4 -
SKHDQIGEVKV SY65_DROME 287 4 -
SRNDVIGKIYL Q62747 362 4 -
KSNDFIGGVSL Q14183 347 4 -
KSNDFIGGVSL P70611 350 4 -
KSNDYIGGCQL RP3A_RAT 636 4 -
KSNDYIGGCQL RP3A_BOVIN 656 4 -
SRDDVIGEVLV SYT4_RAT 249 4 -
SSNDFIGRIVI Q62807 417 4 -
SRNEVIGRLVL SYT4_MOUSE 383 4 -
SRDDVIGEVMV O08835 252 4 -
SKNDFIGEVTL Q20354 200 4 -
KHDDYIGGILL YPT7_CAEEL 978 4 -