| Literature References | 1. DINARELLO, C.A.
The interleukin-1 family - 10 years of discovery.
FASEB J. 8(15) 1314-1325 (1994).
2. HOWARD, A.D., KOSTURA, M.J., THORNBERRY, N., DING, G.J.F., LIMJUCO, G.,
WEIDNER, J., SALLEY, J.P., HOGQUIST, K.A., CHAPLIN, D.D., MUMFORD, R.A.,
SCHMIDT, J.A. AND TOCCI, M.J.
IL-1-converting enzyme requires aspartic acid residues for processing of
the IL-1B precursor at 2 distinct sites and does not cleave 31kDa IL-1A.
J.IMMUNOL. 147(9) 2964-2969 (1991).
3. WANG, L., MIURA, M., BERGERON, L., ZHU, H. AND YUAN, J.Y.
ICH-1, an ICE/CED-3-related gene, encodes both positive and negative
regulators of programmed cell death.
CELL 78(5) 739-750 (1994).
4. KUIDA, K., LIPPKE, J.A., KU, G., HARDING, M.W., LIVINGSTONE, D.J.,
S.-S., MICHAEL AND FLAVELL, R.A.
Altered cytokine export and apoptosis in mice deficient in interleukin-1B
converting enzyme.
SCIENCE 267 2000-2003 (1995).
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| Documentation | Interleukin-1B (IL-1B) converting enzyme (ICE) processes the inactive IL-1B
precursor to the proinflammatory cytokine, which plays a central role in
acute and chronic inflammation, bone resorption, myelogenous leukemia, and
other pathological processes [1]. ICE is a cysteine protease with substrate
cleavage specificity for Asp-X [2]. It is synthesised largely in monocytes
as an inactive proenzyme that autoprocesses to an active tetramer of 2
10kDa and 2 20KDa subunits. The physiological functions of the mammalian
ICE homologues are unknown, but overexpression of ICE and its homologues in
transfected cell lines induces apoptosis [3,4]
IL1BCENZYME is a 7-element fingerprint that provides a signature for
interleukin-1B converting enzymes. The fingerprint was derived from an
initial alignment of 6 sequences: the motifs were drawn from short
conserved regions spanning the C-terminal half of the alignment. Two
iterations on OWL25.2 were required to reach convergence, at which point
a true set comprising 8 sequences was identified. Two partial matches
were also found: HSU13022 is a fragment lacking the final 2 motifs,
and NED2_MOUSE is a murine NEDD-2 sequence that lacks the first 5 motifs.
An update on SPTR37_9f identified a true set of 33 sequences, and 12
partial matches.
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