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PR00365

Identifier
ENDOTHELIN  [View Relations]  [View Alignment]  
Accession
PR00365
No. of Motifs
2
Creation Date
03-JUL-1995  (UPDATE 10-JUN-1999)
Title
Endothelin signature
Database References

PROSITE; PS00270 ENDOTHELIN
BLOCKS; BL00270
PFAM; PF00322 endothelin
INTERPRO; IPR001928
PDB; 1EDP
SCOP; 1EDP
Literature References
1. WATSON, S. AND ARKINSTALL, S.
Endothelin.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, 1994, PP.111-116.

Documentation
Endothelins play an important role in the regulation of the cardiovascular
system [1]. They are the most potent vasoconstrictors known, they stimulate
cardiac constraction, regulate release of vasoactive substances, and 
stimulate mitogenesis in blood vessels in primary culture. They also
stimulate contraction in almost all other smooth muscles (e.g., uterus,
bronchus, vas deferensa and stomach) and stimulate secretion in several
tissues (e.g., kidney, liver and adrenals). Endothelin receptors have also
been found in the brain, e.g. cerebral cortex, cerebellum and glial cells.
Endothelins have been implicated in a variety of pathophysiological
conditions associated with stress, including hypertension, myocardial
infarction, subarachnoid haemorrhage and renal failure.
 
Endothelins are synthesised by proteolysis of large preproendothelins,
which are cleaved to 'big endothelins' before being processed to the
mature peptide [1]. Three distinct human endothelins encoded by separate
genes have been identified: ET1, ET2 and ET3 are present in lung, kidney,
adrenal gland, brain and other tissues. The sequences of the peptides
contain 4 cysteine residues, which are involved in disulphide bond
formation, and are highly similar to the SRTX family from snake venom.
 
ENDOTHELIN is a 3-element fingerprint that provides a signature for the
endothelins. The fingerprint was derived from an initial alignment of 10 
sequences: motifs 1 and 2 span span the full peptide length, and motif 3
lies in the C-terminal preproendothelin region. Two iterations on
SPTR57.15_40.15f were required to reach convergence, at which point a true
set comprising 44 sequences was identified. Two partial matches were also
found, Q08FZ4_DPV83 and Q08FH4_DPV84, both of which are virally-encoded
endothelin sequences that fail to match motif 3. 
Summary Information
16 codes involving  2 elements
Composite Feature Index
21616
12
True Positives
BTX_ATRBI     ET1_BOVIN     ET1_HUMAN     ET1_MOUSE     
ET1_PIG ET1_RABIT ET1_RAT ET2_HUMAN
ET2_MOUSE ET2_RAT ET3_HUMAN ET3_MOUSE
ET3_RAT Q03229 SRTD_ATREN SRTX_ATREN
Sequence Titles
BTX_ATRBI   BIBROTOXIN (BTX) - ATRACTASPIS BIBRONI (SOUTHERN BIBRON'S) (STILETTO SNAKE). 
ET1_BOVIN ENDOTHELIN-1 PRECURSOR (ET-1) - BOS TAURUS (BOVINE).
ET1_HUMAN ENDOTHELIN-1 PRECURSOR (ET-1) - HOMO SAPIENS (HUMAN).
ET1_MOUSE ENDOTHELIN-1 PRECURSOR (ET-1) - MUS MUSCULUS (MOUSE).
ET1_PIG ENDOTHELIN-1 PRECURSOR (ET-1) - SUS SCROFA (PIG).
ET1_RABIT ENDOTHELIN-1 PRECURSOR (ET-1) - ORYCTOLAGUS CUNICULUS (RABBIT).
ET1_RAT ENDOTHELIN-1 PRECURSOR (ET-1) - RATTUS NORVEGICUS (RAT).
ET2_HUMAN ENDOTHELIN-2 PRECURSOR (ET-2) - HOMO SAPIENS (HUMAN).
ET2_MOUSE ENDOTHELIN-2 PRECURSOR (ET-2) (VASOACTIVE INTESTINAL CONTRACTOR) (VIC) - MUS MUSCULUS (MOUSE).
ET2_RAT ENDOTHELIN-2 PRECURSOR (ET-2) (VASOACTIVE INTESTINAL CONTRACTOR) (VIC) - RATTUS NORVEGICUS (RAT).
ET3_HUMAN ENDOTHELIN-3 PRECURSOR (ET-3) - HOMO SAPIENS (HUMAN).
ET3_MOUSE ENDOTHELIN-3 PRECURSOR (ET-3) - MUS MUSCULUS (MOUSE).
ET3_RAT ENDOTHELIN-3 PRECURSOR (ET-3) - RATTUS NORVEGICUS (RAT).
Q03229 ENDOTHELIN-3 PRECURSOR, PLACENTAL SUBTYPE (ET-3) - HOMO SAPIENS (HUMAN).
SRTD_ATREN SARAFOTOXIN-D (S6D) (SRTX-D) - ATRACTASPIS ENGADDENSIS (ISRAELI BURROWING ASP).
SRTX_ATREN SARAFOTOXINS PRECURSOR [CONTAINS: SARAFOTOXIN-A (S6A) (SRTX-A); SARAFOTOXIN-B (S6B) (SRTX-B); SARAFOTOXIN-C (S6C) (SRTX-C); SARAFOTOXIN-E (S6E) (SRTX-E)] - ATRACTASPIS ENGADDENSIS (ISRAELI BURROWING ASP).
Scan History
OWL26_0    2  100  NSINGLE    
SPTR37_9f 2 28 NSINGLE
Initial Motifs
Motif 1  width=10
Element Seqn Id St Int Rpt
SCSSWLDKEC ET2_CANFA 32 32 -
SCKDMTDKEC SRTX_ATREN 151 151 -
TCFTYKDKEC ET3_RABIT 90 90 -
SCSSLMDKEC ET1_BOVIN 54 54 -
SCSSLMDKEC ET1_MOUSE 7 7 -
SCSSWLDKEC ET2_HUMAN 50 50 -
TCFTYKDKEC ET3_HUMAN 98 98 -

Motif 2 width=10
Element Seqn Id St Int Rpt
CVYFCHLDII ET1_MOUSE 16 -1 -
CVYFCHLDII ET2_HUMAN 59 -1 -
CVYYCHLDII ET3_HUMAN 107 -1 -
CVYFCHLDII ET2_CANFA 41 -1 -
CLYFCHQDVI SRTX_ATREN 160 -1 -
CVYYCHLDII ET3_RABIT 99 -1 -
CVYFCHLDII ET1_BOVIN 63 -1 -
Final Motifs
Motif 1  width=10
Element Seqn Id St Int Rpt
SCKDMTDKEC SRTX_ATREN 431 431 R10
SCKDMTDKEC SRTX_ATREN 151 151 R3
TCKDMTDKEC SRTD_ATREN 2 2 -
SCADMTDKEC BTX_ATRBI 2 2 -
SCKDMTDKEC SRTX_ATREN 511 511 R12
SCSSLMDKEC ET1_BOVIN 54 54 -
SCSSLMDKEC ET1_HUMAN 54 54 -
SCSSLMDKEC ET1_MOUSE 7 7 -
SCSSLMDKEC ET1_PIG 54 54 -
SCSSLMDKEC ET1_RABIT 54 54 -
SCSSLMDKEC ET1_RAT 54 54 -
SCKDMSDKEC SRTX_ATREN 471 471 R11
SCKDMSDKEC SRTX_ATREN 311 311 R7
SCKDMSDKEC SRTX_ATREN 71 71 R1
SCSSWLDKEC ET2_HUMAN 50 50 -
SCNSWLDKEC ET2_MOUSE 47 47 -
SCNSWLDKEC ET2_RAT 48 48 -
TCKDMTDKEC SRTX_ATREN 271 271 R6
TCNDMTDEEC SRTX_ATREN 391 391 R9
TCNDMTDEEC SRTX_ATREN 351 351 R8
TCNDMTDEEC SRTX_ATREN 231 231 R5
TCNDMTDEEC SRTX_ATREN 191 191 R4
TCNDMTDEEC SRTX_ATREN 111 111 R2
TCFTYKDKEC ET3_HUMAN 98 98 -
TCFTYKDKEC ET3_MOUSE 98 98 -
TCFTYKDKEC ET3_RAT 54 54 -
TCFTYKDKEC Q03229 98 98 -

Motif 2 width=10
Element Seqn Id St Int Rpt
CLYFCHQDVI SRTX_ATREN 440 -1 R10
CLYFCHQDVI SRTX_ATREN 160 -1 R3
CLYFCHQDII SRTD_ATREN 11 -1 -
CLYFCHQDVI BTX_ATRBI 11 -1 -
CLNFCHQDVI SRTX_ATREN 520 -1 R12
CVYFCHLDII ET1_BOVIN 63 -1 -
CVYFCHLDII ET1_HUMAN 63 -1 -
CVYFCHLDII ET1_MOUSE 16 -1 -
CVYFCHLDII ET1_PIG 63 -1 -
CVYFCHLDII ET1_RABIT 63 -1 -
CVYFCHLDII ET1_RAT 63 -1 -
CLNFCHQDVI SRTX_ATREN 480 -1 R11
CLNFCHQDVI SRTX_ATREN 320 -1 R7
CLNFCHQDVI SRTX_ATREN 80 -1 R1
CVYFCHLDII ET2_HUMAN 59 -1 -
CVYFCHLDII ET2_MOUSE 56 -1 -
CVYFCHLDII ET2_RAT 57 -1 -
CLYFCHQGII SRTX_ATREN 280 -1 R6
CLNFCHQDVI SRTX_ATREN 400 -1 R9
CLNFCHQDVI SRTX_ATREN 360 -1 R8
CLNFCHQDVI SRTX_ATREN 240 -1 R5
CLNFCHQDVI SRTX_ATREN 200 -1 R4
CLNFCHQDVI SRTX_ATREN 120 -1 R2
CVYYCHLDII ET3_HUMAN 107 -1 -
CVYYCHLDII ET3_MOUSE 107 -1 -
CVYYCHLDII ET3_RAT 63 -1 -
CVYYCHLDII Q03229 107 -1 -