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PR00357

Identifier
ANTIFREEZIII  [View Relations]  [View Alignment]  
Accession
PR00357
No. of Motifs
4
Creation Date
11-MAY-1995  (UPDATE 06-JUN-1999)
Title
Type III antifreeze protein signature
Database References

PFAM; PF01354 Antifreeze
INTERPRO; IPR000029
Literature References
1. SONNICHSEN, F.D., SYKES, B.D. AND DAVIES, P.L.
Comparative modelling of the three-dimensional structure of Type II
antifreeze protein.
PROTEIN SCI. 4 460-471 (1995).

Documentation
Marine teleosts from polar oceans can be protected from freezing in icy
sea-water by serum antifreeze proteins (AFPs) or glycoproteins (AFGPs) [1]:
these function by binding to, and preventing the growth of, ice crystals
within the fish. Despite functional similarity, the proteins are
structurally diverse and include glycosylated and at least 3 non-
glycosylated forms: the AFGP of nototheniids and cods are polymers of a
tripeptide repeat, Ala-Ala-Thr, with a disaccharide attached to the
threonine residue; type I AFPs are Ala-rich, alpha-helical peptides
found in flounder and sculpin; type II AFPs of sea-raven, smelt and
herring are Cys-rich proteins; and type III AFPs, found in eel pouts,
are rich in beta-structure.
 
ANTIFREEZIII is a 4-element fingerprint that provides a signature for
type III antifreeze proteins. The fingerprint was derived from an initial
alignment of 10 sequences: the motifs were drawn from conserved regions
spanning the full alignment length. Two iterations on OWL26.0 were
required to reach convergence, at which point a true set comprising 23
sequences was identified. A single partial match was also found, a
type III antifreeze protein fragment lacking the final 2 motifs.
 
An update on SPTR37_9f identified a true set of 20 sequences.
Summary Information
20 codes involving  4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
420202020
30000
20000
1234
True Positives
ANP1_ANALU    ANP1_AUSBR    ANP1_LYCPO    ANP1_MACAM    
ANP1_RHIDE ANP2_ANALU ANP2_AUSBR ANP2_MACAM
ANP2_RHIDE ANP3_MACAM ANP3_RHIDE ANP4_MACAM
ANP5_MACAM ANP7_MACAM ANP9_MACAM ANPB_MACAM
ANPC_MACAM ANPD_MACAM ANPE_MACAM Q92006
Sequence Titles
ANP1_ANALU  ANTIFREEZE PROTEIN TYPE III PRECURSOR - ANARHICHAS LUPUS (ATLANTIC WOLFFISH). 
ANP1_AUSBR ANTIFREEZE PEPTIDE AB1 - AUSTROLYCICHTHYS BRACHYCEPHALUS (ANTARCTIC EEL POUT).
ANP1_LYCPO ANTIFREEZE PROTEIN LP - LYCODES POLARIS (CANADIAN EEL POUT).
ANP1_MACAM ANTIFREEZE GLYCOPROTEIN SP1-C PRECURSOR - MACROZOARCES AMERICANUS (NORTH-ATLANTIC OCEAN POUT).
ANP1_RHIDE ANTIFREEZE PEPTIDE RD1 - RHIGOPHILA DEARBORNI (ANTARCTIC EEL POUT) (LYCODICHTHYS DEARBORNI).
ANP2_ANALU ANTIFREEZE PROTEIN TYPE III PRECURSOR - ANARHICHAS LUPUS (ATLANTIC WOLFFISH).
ANP2_AUSBR ANTIFREEZE PEPTIDE AB2 - AUSTROLYCICHTHYS BRACHYCEPHALUS (ANTARCTIC EEL POUT).
ANP2_MACAM ANTIFREEZE GLYCOPROTEIN SP2(HPLC 1) - MACROZOARCES AMERICANUS (NORTH-ATLANTIC OCEAN POUT).
ANP2_RHIDE ANTIFREEZE PEPTIDE RD2 - RHIGOPHILA DEARBORNI (ANTARCTIC EEL POUT) (LYCODICHTHYS DEARBORNI).
ANP3_MACAM ANTIFREEZE GLYCOPROTEIN LAMBDA OP-3 PRECURSOR - MACROZOARCES AMERICANUS (NORTH-ATLANTIC OCEAN POUT).
ANP3_RHIDE ANTIFREEZE PEPTIDE RD3 - RHIGOPHILA DEARBORNI (ANTARCTIC EEL POUT) (LYCODICHTHYS DEARBORNI).
ANP4_MACAM ANTIFREEZE GLYCOPROTEIN SP1(HPLC 4) - MACROZOARCES AMERICANUS (NORTH-ATLANTIC OCEAN POUT).
ANP5_MACAM ANTIFREEZE GLYCOPROTEIN LAMBDA OP-5 PRECURSOR - MACROZOARCES AMERICANUS (NORTH-ATLANTIC OCEAN POUT).
ANP7_MACAM ANTIFREEZE GLYCOPROTEIN SP4(HPLC 7) - MACROZOARCES AMERICANUS (NORTH-ATLANTIC OCEAN POUT).
ANP9_MACAM ANTIFREEZE GLYCOPROTEIN SP3(HPLC 9) - MACROZOARCES AMERICANUS (NORTH-ATLANTIC OCEAN POUT).
ANPB_MACAM ANTIFREEZE GLYCOPROTEIN SP2(HPLC 11) - MACROZOARCES AMERICANUS (NORTH-ATLANTIC OCEAN POUT).
ANPC_MACAM ANTIFREEZE GLYCOPROTEIN QAE(HPLC 12) - MACROZOARCES AMERICANUS (NORTH-ATLANTIC OCEAN POUT).
ANPD_MACAM ANTIFREEZE GLYCOPROTEIN C7 PRECURSOR - MACROZOARCES AMERICANUS (NORTH-ATLANTIC OCEAN POUT).
ANPE_MACAM ANTIFREEZE GLYCOPROTEIN C10 PRECURSOR - MACROZOARCES AMERICANUS (NORTH-ATLANTIC OCEAN POUT).
Q92006 PRE-ANTIFREEZE PEPTIDE RD2 PRECURSOR - RHIGOPHILA DEARBORNI (ANTARCTIC EEL POUT) (LYCODICHTHYS DEARBORNI).
Scan History
OWL26_0    2  100  NSINGLE    
SPTR37_9f 2 22 NSINGLE
Initial Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
VVATQLIPINTALTPIM ANP1_ANALU 26 26 -
VVATQLIPMNSALTPVM ANPE_MACAM 25 25 -
VVANQLIPINTALTLVM ANP3_RHIDE 75 75 -
VVANQLIPINTALTLVM ANPC_MACAM 5 5 -
VVATQLIPINTALTPAM ANPB_MACAM 2 2 -
VVATQLIPINTALTPVM ANP5_MACAM 25 25 -
VVATQLIPMNTALTPAM ANP9_MACAM 4 4 -
VVATQLIPINTALTPVM ANPD_MACAM 25 25 -
VVATQLIPINTALTPIM ANP2_ANALU 26 26 -
VVATQLIPINTALTPAM ANP4_MACAM 3 3 -

Motif 2 width=17
Element Seqn Id St Int Rpt
MEGKVTNPIGIPFAEMS ANPE_MACAM 42 0 -
MKAEEVSPKGIPSEEIS ANP3_RHIDE 92 0 -
MEGKVTNPIGIPFAEMS ANPB_MACAM 19 0 -
MEGKVTNPIGIPFAEMS ANP5_MACAM 42 0 -
MEGKVTNPIGIPFAEMS ANP9_MACAM 21 0 -
MEGKVTNPIGIPFAEMS ANPD_MACAM 42 0 -
MKGQVVNPAGIPFAEMS ANP2_ANALU 43 0 -
MEGKVTNPIGIPFAEMS ANP4_MACAM 20 0 -
MKGQVVNPAGIPFAEMS ANP1_ANALU 43 0 -
MRSEVVTPVGIPAEDIP ANPC_MACAM 22 0 -

Motif 3 width=16
Element Seqn Id St Int Rpt
KLVGMQVNRAVYLDQT ANP3_RHIDE 109 0 -
QIVGKQVNRIVAKGQT ANPB_MACAM 36 0 -
QIVGKQVNTPVAKGQT ANP5_MACAM 59 0 -
QIVGKQVNRIVAKGQT ANP9_MACAM 38 0 -
QIVGKQVNTPVAKGQT ANPD_MACAM 59 0 -
QIVGKQVNRAVAKDET ANP2_ANALU 60 0 -
QIVGKQVNTPVAKGQT ANP4_MACAM 37 0 -
QIVGKQVNRPVAKDET ANP1_ANALU 60 0 -
QMVGKQVNRPVAKGQT ANPE_MACAM 59 0 -
RLVSMQVNRAVPLGTT ANPC_MACAM 39 0 -

Motif 4 width=10
Element Seqn Id St Int Rpt
TIMPNMVKTY ANPE_MACAM 74 -1 -
TLMPDMVKGY ANPC_MACAM 54 -1 -
TLMPNMVKTY ANPB_MACAM 51 -1 -
TLMPDMVKNY ANP3_RHIDE 124 -1 -
TLMPNMVKTY ANP5_MACAM 74 -1 -
TLMPNMVKTY ANP9_MACAM 53 -1 -
TIMPNMVKTY ANPD_MACAM 74 -1 -
TLMPNMVKTY ANP2_ANALU 75 -1 -
TIMPNMVKTY ANP4_MACAM 52 -1 -
TLMPNMVKTY ANP1_ANALU 75 -1 -
Final Motifs
Motif 1  width=17
Element Seqn Id St Int Rpt
VVATQLIPINTALTPAM ANPB_MACAM 2 2 -
VVATQLIPINTALTPVM ANP5_MACAM 25 25 -
VVATQLIPINTALTPAM ANP1_MACAM 25 25 -
VVATQLIPMNTALTPAM ANP9_MACAM 4 4 -
VVATQLIPINTALTPVM ANPD_MACAM 25 25 -
VVATQLIPINTALTPIM ANP2_ANALU 26 26 -
VVATQLIPINTALTPAM ANP4_MACAM 3 3 -
VVATRLIPMNTALTPAM ANP7_MACAM 4 4 -
VVATQLIPINTALTPIM ANP1_ANALU 26 26 -
VVATQLIPMNTALTPVM ANP2_MACAM 4 4 -
VVATQLIPMNSALTPVM ANPE_MACAM 25 25 -
VVANQLIPINTALTLIM ANP1_RHIDE 5 5 -
VVANQLIPINTALTLIM ANP3_RHIDE 5 5 R1
VVASQLIPINTALTPAM ANP1_AUSBR 4 4 -
VVANQLIPINTALTLIM ANP2_RHIDE 5 5 -
VVANQLIPINTALTLIM Q92006 27 27 -
VVANQLIPINTALTLVM ANP2_AUSBR 4 4 -
VVANQLIPINTALTLVM ANP3_RHIDE 75 75 R2
VVANQLIPINTALTLVM ANP1_LYCPO 5 5 -
VVANQLIPINTALTLVM ANPC_MACAM 5 5 -
VVATQLIPINTALTLVM ANP3_MACAM 26 26 -

Motif 2 width=17
Element Seqn Id St Int Rpt
MEGKVTNPIGIPFAEMS ANPB_MACAM 19 0 -
MEGKVTNPIGIPFAEMS ANP5_MACAM 42 0 -
MEGKVTNPIGIPFAEMS ANP1_MACAM 42 0 -
MEGKVTNPIGIPFAEMS ANP9_MACAM 21 0 -
MEGKVTNPIGIPFAEMS ANPD_MACAM 42 0 -
MKGQVVNPAGIPFAEMS ANP2_ANALU 43 0 -
MEGKVTNPIGIPFAEMS ANP4_MACAM 20 0 -
MEGKVTNPIGIPFAEMS ANP7_MACAM 21 0 -
MKGQVVNPAGIPFAEMS ANP1_ANALU 43 0 -
MEGKVTNPIGIPFAEMS ANP2_MACAM 21 0 -
MEGKVTNPIGIPFAEMS ANPE_MACAM 42 0 -
MKAEVVTPMGIPAEEIP ANP1_RHIDE 22 0 -
MKAEVVTPMGIPAEEIP ANP3_RHIDE 22 0 R1
MKAKEVSPKGIPAEEMS ANP1_AUSBR 21 0 -
MKAEVVTPMGIPAEDIP ANP2_RHIDE 22 0 -
MKAEVVTPMGIPAEDIP Q92006 44 0 -
MKAEEVSPKGIPAEEIP ANP2_AUSBR 21 0 -
MKAEEVSPKGIPSEEIS ANP3_RHIDE 92 0 R2
MRAEVVTPAGIPAEDIP ANP1_LYCPO 22 0 -
MRSEVVTPVGIPAEDIP ANPC_MACAM 22 0 -
MTTRVIYPTGIPAEDIP ANP3_MACAM 43 0 -

Motif 3 width=16
Element Seqn Id St Int Rpt
QIVGKQVNRIVAKGQT ANPB_MACAM 36 0 -
QIVGKQVNTPVAKGQT ANP5_MACAM 59 0 -
QIVGKQVNTPVAKGQT ANP1_MACAM 59 0 -
QIVGKQVNRIVAKGQT ANP9_MACAM 38 0 -
QIVGKQVNTPVAKGQT ANPD_MACAM 59 0 -
QIVGKQVNRAVAKDET ANP2_ANALU 60 0 -
QIVGKQVNTPVAKGQT ANP4_MACAM 37 0 -
QIVGKQVNRIVAKGQT ANP7_MACAM 38 0 -
QIVGKQVNRPVAKDET ANP1_ANALU 60 0 -
QIVGKQVNTPVAKGQT ANP2_MACAM 38 0 -
QMVGKQVNRPVAKGQT ANPE_MACAM 59 0 -
KLVGMQVNRAVPLGTT ANP1_RHIDE 39 0 -
NLVGMQVNRAVPLGTT ANP3_RHIDE 39 0 R1
KIVGMQVNRAVNLDET ANP1_AUSBR 38 0 -
RIIGMQVNRAVPLGTT ANP2_RHIDE 39 0 -
RIIGMQVNRAVPLGTT Q92006 61 0 -
RLVGMQVNRAVYLDET ANP2_AUSBR 38 0 -
KLVGMQVNRAVYLDQT ANP3_RHIDE 109 0 R2
RLVGLQVNRAVLIGTT ANP1_LYCPO 39 0 -
RLVSMQVNRAVPLGTT ANPC_MACAM 39 0 -
RLVSMQVNQAVPMGTT ANP3_MACAM 60 0 -

Motif 4 width=10
Element Seqn Id St Int Rpt
TLMPNMVKTY ANPB_MACAM 51 -1 -
TLMPNMVKTY ANP5_MACAM 74 -1 -
TLMPNMVKTY ANP1_MACAM 74 -1 -
TLMPNMVKTY ANP9_MACAM 53 -1 -
TIMPNMVKTY ANPD_MACAM 74 -1 -
TLMPNMVKTY ANP2_ANALU 75 -1 -
TIMPNMVKTY ANP4_MACAM 52 -1 -
TLMPNMVKTY ANP7_MACAM 53 -1 -
TLMPNMVKTY ANP1_ANALU 75 -1 -
TIMPNMVKTY ANP2_MACAM 53 -1 -
TIMPNMVKTY ANPE_MACAM 74 -1 -
TLMPDMVKNY ANP1_RHIDE 54 -1 -
TLMPDMVKNY ANP3_RHIDE 54 -1 R1
TLMPDMVKTY ANP1_AUSBR 53 -1 -
TLMPDMVKNY ANP2_RHIDE 54 -1 -
TLMPDMVKNY Q92006 76 -1 -
TLMPDMVKNY ANP2_AUSBR 53 -1 -
TLMPDMVKNY ANP3_RHIDE 124 -1 R2
TLMPDMVKGY ANP1_LYCPO 54 -1 -
TLMPDMVKGY ANPC_MACAM 54 -1 -
TLMPDMVKFY ANP3_MACAM 75 -1 -