| Literature References | 1. PEARSON, J.D., DEWALD, D.B., MATHEWS, W.R., MOZIER, N.M., ZURCHER-NEELY,
H.A., HEINRIKSON, R.L., MORRIS, M.A., MCCUBBIN, W.D., MCDONALD, J.R.,
FRASER, E.D., VOGEL, H.J., KAY, C.M. AND WALSH, M.P.
Amino acid sequence and characterization of a protein inhibitor of protein
kinase C.
J.BIOL.CHEM. 265 4583-4591 (1990).
2. MOZIER, N.M., WALSH, M.P. AND PEARSON, J.D.
Characterization of a novel zinc binding site of protein kinase C
inhibitor-1.
FEBS LETT. 279 14-18 (1991).
3. SERAPHIN, B.
The HIT protein family: a new family of proteins present in prokaryotes,
yeast and mammals.
DNA SEQ. 3 177-179 (1992).
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| Documentation | The bovine protein kinase C inhibitor, PKCI-1, is an inhibitor protein that
binds zinc without the use of zinc-finger motifs [1]. Each protein molecule
binds one zinc ion via a novel binding site containing 3 closely-spaced
histidine residues [2]. This region, refered to as the histidine triad
(HIT) [2], has been identified in various prokaryotic and eukaryotic
proteins of uncertain function [3].
HISTRIAD is a 3-element fingerprint that provides a signature for the HIT
protein family. The fingerprint was derived from an initial alignment
of 5 sequences: motifs 1 and 2 were drawn from conserved regions towards
the N-terminus, and motif 3 includes part of the region encoded by PROSITE
pattern HIT (PS00892), the 3 conserved His residues of which are thought
to bind the zinc ion. Two iterations on OWL24.0 were required to reach
convergence, at which point a true set comprising 8 sequences was
identified.
An update on SPTR37_9f identified a true set of 25 sequences, and 6
partial matches.
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| Sequence Titles | HITA_NEIGO HITA PROTEIN - NEISSERIA GONORRHOEAE.
HIT_BACSU HIT PROTEIN - BACILLUS SUBTILIS.
HNT1_YEAST HIT FAMILY PROTEIN 1 - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
IPK1_BOVIN HINT PROTEIN (PROTEIN KINASE C INHIBITOR 1) (PKCI-1) (17 KD INHIBITOR OF PROTEIN KINASE C) - BOS TAURUS (BOVINE), AND RATTUS NORVEGICUS (RAT).
IPK1_HUMAN HINT PROTEIN (PROTEIN KINASE C INHIBITOR 1) (PKCI-1) - HOMO SAPIENS (HUMAN).
IPK1_MOUSE HINT PROTEIN (PROTEIN KINASE C INHIBITOR 1) (PKCI-1) - MUS MUSCULUS (MOUSE).
IPK1_RABIT HINT PROTEIN (PROTEIN KINASE C INHIBITOR 1) (PKCI-1) - ORYCTOLAGUS CUNICULUS (RABBIT).
O66536 PROTEIN KINASE C INHIBITOR (HIT FAMILY) - AQUIFEX AEOLICUS.
O84390 HIT FAMILY HYDROLASE - CHLAMYDIA TRACHOMATIS.
PKIA_DICDI PKIA PROTEIN - DICTYOSTELIUM DISCOIDEUM (SLIME MOLD).
Y866_METJA HYPOTHETICAL HIT-LIKE PROTEIN MJ0866 - METHANOCOCCUS JANNASCHII.
YCFF_ECOLI HYPOTHETICAL 13.2 KD PROTEIN HIT-LIKE PROTEIN IN FHUE 5'REGION - ESCHERICHIA COLI.
YCFF_HAEIN HYPOTHETICAL HIT-LIKE PROTEIN HI0961 - HAEMOPHILUS INFLUENZAE.
YHIT_AZOBR HYPOTHETICAL 13.2 KD HIT-LIKE PROTEIN IN HISE 3'REGION (ORF2) - AZOSPIRILLUM BRASILENSE.
YHIT_BORBU HYPOTHETICAL 15.9 KD HIT-LIKE PROTEIN - BORRELIA BURGDORFERI (LYME DISEASE SPIROCHETE).
YHIT_CAEEL HYPOTHETICAL HIT-LIKE PROTEIN F21C3.3 - CAENORHABDITIS ELEGANS.
YHIT_HELPY HYPOTHETICAL HIT-LIKE PROTEIN HP0404 - HELICOBACTER PYLORI (CAMPYLOBACTER PYLORI).
YHIT_MYCGE HYPOTHETICAL HIT-LIKE PROTEIN MG132 - MYCOPLASMA GENITALIUM.
YHIT_MYCHR HYPOTHETICAL 13.1 KD HIT-LIKE PROTEIN IN P37 5'REGION - MYCOPLASMA HYORHINIS.
YHIT_MYCLE HYPOTHETICAL HIT-LIKE PROTEIN U296A - MYCOBACTERIUM LEPRAE.
YHIT_MYCPN HYPOTHETICAL 16.1 KD PROTEIN HIT-LIKE PROTEIN - MYCOPLASMA PNEUMONIAE.
YHIT_MYCTU HYPOTHETICAL 15.7 KD HIT-LIKE PROTEIN - MYCOBACTERIUM TUBERCULOSIS.
YHIT_SYNP7 HYPOTHETICAL 12.4 KD HIT-LIKE PROTEIN IN PSBAII 5'REGION (ORF 1) - SYNECHOCOCCUS SP. (STRAIN PCC 7942) (ANACYSTIS NIDULANS R2).
YHIT_SYNY3 HYPOTHETICAL HIT-LIKE PROTEIN SLR1234 - SYNECHOCYSTIS SP. (STRAIN PCC 6803).
ZB14_MAIZE 14 KD ZINC-BINDING PROTEIN (PROTEIN KINASE C INHIBITOR) (PKCI) - ZEA MAYS (MAIZE).
APH1_SCHPO BIS(5'-NUCLEOSYL)-TETRAPHOSPHATASE (ASYMMETRICAL) (EC 3.6.1.17) (DIADENOSINE 5',5'''-P1,P4-TETRAPHOSPHATE ASYMMETRICAL HYDROLASE) (DIADENOSINE TETRAPHOSPHATASE) (AP4A HYDROLASE) (AP4AASE) - SCHIZOSACCHAROMYCES POMBE (FISSION YEAST).
O58729 150AA LONG HYPOTHETICAL PROTEIN - PYROCOCCUS HORIKOSHII.
O76463 NITRILASE AND FRAGILE HISTIDINE TRIAD FUSION PROTEIN NITFHIT - CAENORHABDITIS ELEGANS.
P71816 HYPOTHETICAL 11.9 KD PROTEIN CY369.04C - MYCOBACTERIUM TUBERCULOSIS.
P95937 HIT PROTEIN - SULFOLOBUS SOLFATARICUS.
Q18227 SIMILAR TO PROTEIN KINASE C INHIBITORS - CAENORHABDITIS ELEGANS.
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