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PR00322

Identifier
G10  [View Relations]  [View Alignment]  
Accession
PR00322
No. of Motifs
5
Creation Date
10-JUN-1994  (UPDATE 07-JUN-1999)
Title
G10 protein signature
Database References

PROSITE; PS00997 G10_1; PS00998 G10_2
BLOCKS; BL00997
PFAM; PF01125 G10
INTERPRO; IPR001748
Literature References
1. MCGREW, L.L., DWORKIN-RASTL, E., DWORKIN, M.B. AND RICHTER, J.D.
Poly(A) elongation during Xenopus oocyte maturation is required for
translational recruitment and is mediated by a short sequence element.
GENES DEV. 3(6) 803-815 (1989).

Documentation
G10 is a hydrophilic protein containing 143-157 residues, whose C-terminus
is rich in cysteine residues [1]. The function of G10 is unknown, but the 
amino acid sequence is highly conserved in a wide range of eukaryotic 
species, and the Cys-rich region could be involved in metal binding. 
 
G10 is a 5-element fingerprint that provides a signature for G10 proteins.
The fingerprint was derived from an initial alignment of 3 sequences: the
motifs were drawn from the full alignment length, motifs 4 and 5 including
the Cys-rich regions encoded by PROSITE patterns G10_1 (PS00997) and G10_2
(PS00998). Two iterations on OWL23.0 were required to reach convergence,
at which point a true set comprising 4 sequences was identified. 
 
An update on SPTR37_9f identified a true set of 8 sequences.
Summary Information
8 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
588888
400000
300000
200000
12345
True Positives
G10_CAEEL     G10_HUMAN     G10_ORYSA     G10_XENLA     
G10_YEAST O49553 O70454 O74772
Sequence Titles
G10_CAEEL   G10 PROTEIN HOMOLOG - CAENORHABDITIS ELEGANS. 
G10_HUMAN G10 PROTEIN HOMOLOG (EDG-2) - HOMO SAPIENS (HUMAN).
G10_ORYSA G10 PROTEIN HOMOLOG - ORYZA SATIVA (RICE).
G10_XENLA G10 PROTEIN - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
G10_YEAST G10 PROTEIN HOMOLOG - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
O49553 G10 - LIKE PROTEIN - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O70454 G10 PROTEIN HOMOLOG - RATTUS NORVEGICUS (RAT).
O74772 G10 PROTEIN HOMOLOG - SCHIZOSACCHAROMYCES POMBE (FISSION YEAST).
Scan History
OWL23_0    2  20   NSINGLE    
OWL26_0 1 20 NSINGLE
SPTR37_9f 2 9 NSINGLE
Initial Motifs
Motif 1  width=21
Element Seqn Id St Int Rpt
PDGWELIEPTLDELDQKMREA G10_XENLA 12 12 -
PEGWDLIEPTLEQFEAKMREA G10_CAEEL 15 15 -
PDGFEKIKPTLTDFEIQLRDA G10_YEAST 13 13 -

Motif 2 width=24
Element Seqn Id St Int Rpt
WPIFRIHHQKTRYIFDLFYKRKAI G10_XENLA 47 14 -
WPIFRIHHQRSRYVYDMYYKKAEI G10_CAEEL 50 14 -
WEIMQLHHQRSRYIYTLYYKRKAI G10_YEAST 50 16 -

Motif 3 width=26
Element Seqn Id St Int Rpt
SRELYDYCIREGYADKNLIAKWKKQG G10_XENLA 71 0 -
SRELYEFCLTAKFADAALIAKWKKQG G10_CAEEL 74 0 -
SKDLYDWLIKEKYADKLLIAKWRKTG G10_YEAST 74 0 -

Motif 4 width=26
Element Seqn Id St Int Rpt
YENLCCLRCIQTRDTNFGTNCICRVP G10_XENLA 97 0 -
YENLCCVKCVNTRDSNFGTACICRVP G10_CAEEL 100 0 -
YEKLCCLRCIQKNETNNGSTCICRVP G10_YEAST 100 0 -

Motif 5 width=10
Element Seqn Id St Int Rpt
CTHCGCRGCS G10_XENLA 134 11 -
CVHCGCHGCS G10_CAEEL 137 11 -
CVHCGCRGCA G10_YEAST 145 19 -
Final Motifs
Motif 1  width=21
Element Seqn Id St Int Rpt
PDGWELIEPTLDELDQKMREA G10_XENLA 12 12 -
PDGWELIEPTLDELDQKMREA O70454 12 12 -
QDGWELIEPTLDQLDQKMREA G10_HUMAN 12 12 -
PEGWELIEPTLRELDAKMREA O49553 12 12 -
IRWWELIEPTLSELHSKMREA G10_ORYSA 11 11 -
PEGWDLIEPTLEQFEAKMREA G10_CAEEL 15 15 -
PDGFDEIEPTLIEFQDRMRQI O74772 13 13 -
PDGFEKIKPTLTDFEIQLRDA G10_YEAST 13 13 -

Motif 2 width=24
Element Seqn Id St Int Rpt
WPIFRIHHQKTRYIFDLFYKRKAI G10_XENLA 47 14 -
WPIFRIHHQKTRYIFDLFYKRKAI O70454 47 14 -
WPIFRIHHQKTRYIFDLFYKRKAY G10_HUMAN 47 14 -
WPIFKVSHQRSRYVYDLYYRREEI O49553 47 14 -
WPIFKINHQRSRYLYDLYYNRKEI G10_ORYSA 46 14 -
WPIFRIHHQRSRYVYDMYYKKAEI G10_CAEEL 50 14 -
APIFQLHHQRSRYIYDLYYKREAI O74772 47 13 -
WEIMQLHHQRSRYIYTLYYKRKAI G10_YEAST 50 16 -

Motif 3 width=26
Element Seqn Id St Int Rpt
SRELYDYCIREGYADKNLIAKWKKQG G10_XENLA 71 0 -
SRELYEYCIKEGYADKNLIAKWKKQG O70454 71 0 -
SRELLDICYKEGLADKNLLAKWKKQG G10_HUMAN 71 0 -
SKELYEFCLDQGYADRSLIAKWKKSG O49553 71 0 -
SQELYEFCLDQGHADRNLIAKWKKQG G10_ORYSA 70 0 -
SRELYEFCLTAKFADAALIAKWKKQG G10_CAEEL 74 0 -
STELYNWLLKQNYADGNLIAKWKKPG O74772 71 0 -
SKDLYDWLIKEKYADKLLIAKWRKTG G10_YEAST 74 0 -

Motif 4 width=26
Element Seqn Id St Int Rpt
YENLCCLRCIQTRDTNFGTNCICRVP G10_XENLA 97 0 -
YENLCCLRCIQTRDTNFGTNCICRVP O70454 97 0 -
IGNLCCLRCIQTRDTNFGTNCICRVP G10_HUMAN 97 0 -
YERLCCLRCIQPRDHNYGTTCVCRVP O49553 97 0 -
YERLCCLRCIQTRDHNFATTCVCRVP G10_ORYSA 96 0 -
YENLCCVKCVNTRDSNFGTACICRVP G10_CAEEL 100 0 -
YEKLCCLRCIQTAESKFGSTCICRVP O74772 97 0 -
YEKLCCLRCIQKNETNNGSTCICRVP G10_YEAST 100 0 -

Motif 5 width=10
Element Seqn Id St Int Rpt
CTHCGCRGCS G10_XENLA 134 11 -
CTHCGCRGWS O70454 134 11 -
CTHCGCRGCS G10_HUMAN 134 11 -
CVHCGCQGCA O49553 133 10 -
CVHCGCKGCA G10_ORYSA 131 9 -
CVHCGCHGCS G10_CAEEL 137 11 -
CTHCGCNGCA O74772 134 11 -
CVHCGCRGCA G10_YEAST 145 19 -