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PR00296

Identifier
CYCLINKINASE  [View Relations]  [View Alignment]  
Accession
PR00296
No. of Motifs
4
Creation Date
07-OCT-1994  (UPDATE 07-JUN-1999)
Title
Cyclin-dependent kinase regulatory subunit signature
Database References

PROSITE; PS00944 CKS_1; PS00945 CKS_2
BLOCKS; BL00944
PFAM; PF01111 CKS
INTERPRO; IPR000789
Literature References
1. BRIZUELA, L., DRAETTA, G. AND BEACH, D.
p13suc1 acts in the fission yeast cell division cycle as a component of the
p34cdc2 protein kinase.
EMBO J. 6 3507-3514 (1987).
 
2. PARGE, H.E., ARVAI, A.S., MURTARI, D.J., REED, S.I. AND TAINER, J.A.
Human CksHs2 atomic structure: a role for its hexameric assembly in cell 
cycle control.
SCIENCE 262 387-395 (1993).
 
3. TANG, Y. AND REED, S.I.
The Cdk-associated protein Cks1 functions both in G1 and G2 in Saccharomyces
cerevisiae.
GENES DEV. 7 822-832 (1993).

Documentation
In eukaryotes, cyclin-dependent protein kinases interact with cyclins to
regulate cell cycle progression, and are required for the G1 and G2 stages
of cell division [1]. The proteins bind to a regulatory subunit (cyclin-
dependent kinase regulatory subunit, or CKS), which is essential for their
function [2]. The regulatory subunits exist as hexamers, formed by the
symmetrical assembly of 3 interlocked homodimers, creating an unusual
12-stranded beta-barrel structure [2]. Through the barrel centre runs a
12A diameter tunnel, lined by 6 exposed helix pairs [3]. Six kinase units
can be modelled to bind the hexameric structure, which may thus act as a
hub for cyclin-dependent protein kinase multimerisation [2,3].
 
CYCLINKINASE is a 4-element fingerprint that provides a signature for
cyclin-dependent kinase regulatory subunits. The fingerprint was derived
from an initial alignment of 4 sequences: the motifs were drawn from
conserved regions encompassing virtually the full alignment length, motifs
1, 2 and 4 spanning the regions encoded by PROSITE patterns CKS_1 (PS00944)
and CKS_2 (PS00945). Two iterations on OWL24.0 were required to reach
convergence, at which point a true set comprising 5 sequences was
identified.
 
An update on SPTR37_9f identified a true set of 13 sequences.
Summary Information
13 codes involving  4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
413131313
30000
20000
1234
True Positives
CKS1_CAEEL    CKS1_DROME    CKS1_HUMAN    CKS1_LEIME    
CKS1_PATVU CKS1_PHYPO CKS1_SCHPO CKS1_YEAST
CKS2_HUMAN CKS2_MOUSE CKS2_XENLA O23249
O60191
Sequence Titles
CKS1_CAEEL  CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT - CAENORHABDITIS ELEGANS. 
CKS1_DROME CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT - DROSOPHILA MELANOGASTER (FRUIT FLY).
CKS1_HUMAN CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT 1 (CKS-1) - HOMO SAPIENS (HUMAN), AND MUS MUSCULUS (MOUSE).
CKS1_LEIME CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT (P12LMMCKS1) - LEISHMANIA MEXICANA.
CKS1_PATVU CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT (SUC1 HOMOLOG) - PATELLA VULGATA (COMMON LIMPET).
CKS1_PHYPO PROBABLE CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT - PHYSARUM POLYCEPHALUM (SLIME MOLD).
CKS1_SCHPO CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT (P13) - SCHIZOSACCHAROMYCES POMBE (FISSION YEAST).
CKS1_YEAST CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT (CELL DIVISION CONTROL PROTEIN CKS1) - SACCHAROMYCES CEREVISIAE (BAKER'S YEAST).
CKS2_HUMAN CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT 2 (CKS-2) - HOMO SAPIENS (HUMAN).
CKS2_MOUSE CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT 2 (CKS-2) - MUS MUSCULUS (MOUSE).
CKS2_XENLA CYCLIN-DEPENDENT KINASES REGULATORY SUBUNIT 2 (XE-P9) - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
O23249 CKS1 PROTEIN - ARABIDOPSIS THALIANA (MOUSE-EAR CRESS).
O60191 CDC2 BINDING PROTEIN SUC1 - PNEUMOCYSTIS CARINII F. SP. CARINII.
Scan History
OWL24_0    2  100  NSINGLE    
OWL28_0 1 100 NSINGLE
SPTR37_9f 2 14 NSINGLE
Initial Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
QIYYSDKYDDEEFEY CKS1_HUMAN 5 5 -
QIYYSDKYFDEHYEY CKS2_HUMAN 5 5 -
QIHYSPRYADDEYEY CKS1_SCHPO 24 24 -
SIHYSPRYSDDNYEY CKS1_YEAST 27 27 -

Motif 2 width=15
Element Seqn Id St Int Rpt
RHVMLPKDIAKLVPK CKS1_HUMAN 20 0 -
RHVMLPRELSKQVPK CKS2_HUMAN 20 0 -
RHVMLPKAMLKAIPT CKS1_SCHPO 39 0 -
RHVMLPKAMLKVIPS CKS1_YEAST 42 0 -

Motif 3 width=15
Element Seqn Id St Int Rpt
EWRNLGVQQSQGWVH CKS1_HUMAN 42 7 -
EWRRLGVQQSLGWVH CKS2_HUMAN 42 7 -
EWRGLGITQSLGWEM CKS1_SCHPO 70 16 -
EWRGLGITQSLGWEH CKS1_YEAST 73 16 -

Motif 4 width=15
Element Seqn Id St Int Rpt
YMIHEPEPHILLFRR CKS1_HUMAN 57 0 -
YMIHEPEPHILLFRR CKS2_HUMAN 57 0 -
YEVHVPEPHILLFKR CKS1_SCHPO 85 0 -
YECHAPEPHILLFKR CKS1_YEAST 88 0 -
Final Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
NIYYSDKYTDEHFEY CKS2_XENLA 5 5 -
QIYYSDKYDDEEFEY CKS1_HUMAN 5 5 -
QIYYSDKYFDEHYEY CKS2_HUMAN 5 5 -
QIYYSDKYFDEHYEY CKS2_MOUSE 5 5 -
QIYYSDKYFDEDFEY CKS1_PATVU 5 5 -
DIYYSDKYYDEQFEY CKS1_DROME 4 4 -
TIQYSEKYYDDKFEY CKS1_PHYPO 5 5 -
KILYSDKYYDDMFEY CKS1_LEIME 30 30 -
QIQYSEKYFDDTFEY O23249 3 3 -
NIHYSTRYSDDTHEY O60191 27 27 -
QIHYSPRYADDEYEY CKS1_SCHPO 24 24 -
SIHYSPRYSDDNYEY CKS1_YEAST 27 27 -
DFYYSNKYEDDEFEY CKS1_CAEEL 7 7 -

Motif 2 width=15
Element Seqn Id St Int Rpt
RHVMLPKELAKQVPK CKS2_XENLA 20 0 -
RHVMLPKDIAKLVPK CKS1_HUMAN 20 0 -
RHVMLPRELSKQVPK CKS2_HUMAN 20 0 -
RHVMLPRELSKQVPK CKS2_MOUSE 20 0 -
RHVMLPKDIAKMVPK CKS1_PATVU 20 0 -
RHVVLPKELVKMVPK CKS1_DROME 19 0 -
RHVILPPDVAKEIPK CKS1_PHYPO 20 0 -
RHVILPKDLARLVPT CKS1_LEIME 45 0 -
RHVVLPPEVAKLLPK O23249 18 0 -
RHVILPKPMLKAIPK O60191 42 0 -
RHVMLPKAMLKAIPT CKS1_SCHPO 39 0 -
RHVMLPKAMLKVIPS CKS1_YEAST 42 0 -
RHVHVTKDVSKLIPK CKS1_CAEEL 22 0 -

Motif 3 width=15
Element Seqn Id St Int Rpt
EWRRLGVQQSLGWVH CKS2_XENLA 42 7 -
EWRNLGVQQSQGWVH CKS1_HUMAN 42 7 -
EWRRLGVQQSLGWVH CKS2_HUMAN 42 7 -
EWRRLGVQQSLGWVH CKS2_MOUSE 42 7 -
EWRSIGVQQSHGWIH CKS1_PATVU 42 7 -
EWRSIGVQQSRGWIH CKS1_DROME 41 7 -
EWRGLGVQQSQGWVH CKS1_PHYPO 42 7 -
EWRQLGVQQSQGWVH CKS1_LEIME 67 7 -
EWRAIGVQQSRGWVH O23249 40 7 -
EWRGLGITQSLGWQH O60191 73 16 -
EWRGLGITQSLGWEM CKS1_SCHPO 70 16 -
EWRGLGITQSLGWEH CKS1_YEAST 73 16 -
EWRSLGIQQSPGWMH CKS1_CAEEL 44 7 -

Motif 4 width=15
Element Seqn Id St Int Rpt
YMIHEPEPHILLFRR CKS2_XENLA 57 0 -
YMIHEPEPHILLFRR CKS1_HUMAN 57 0 -
YMIHEPEPHILLFRR CKS2_HUMAN 57 0 -
YMIHEPEPHILLFRR CKS2_MOUSE 57 0 -
YMKHEPEPHILLFRR CKS1_PATVU 57 0 -
YMIHKPEPHILLFRR CKS1_DROME 56 0 -
YALHRPEPHILLFRR CKS1_PHYPO 57 0 -
YMIHKPEPHVLLFKR CKS1_LEIME 82 0 -
YAVHRPEPHIMLFRR O23249 55 0 -
YEIHVPEPHILLFKR O60191 88 0 -
YEVHVPEPHILLFKR CKS1_SCHPO 85 0 -
YECHAPEPHILLFKR CKS1_YEAST 88 0 -
YMIHGPERHVLLFRR CKS1_CAEEL 59 0 -