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PR00290

Identifier
KAZALINHBTR  [View Relations]  [View Alignment]  
Accession
PR00290
No. of Motifs
2
Creation Date
20-JAN-1995  (UPDATE 21-JUN-1999)
Title
Kazal-type serine protease inhibitor signature
Database References

PROSITE; PS00282 KAZAL
BLOCKS; BL00282
PFAM; PF00050 kazal
INTERPRO; IPR001239
PDB; 1BUS
SCOP; 1BUS
CATH; 1BUS
Literature References
1. WILLIAMSON, M.P., MARION, D. AND WUTHRICH, K.
Secondary structure in the solution conformation of the proteinase
inhibitor IIA from bull seminal plasma by nuclear magnetic resonance.
J.MOL.BIOL. 173 341-359 (1984).
 
2. LASKOWSKI, M., KATO, I., ARDELT, W., COOK, J., DENTON, A. EMPIE, M.W.,
KOHR, W.J., PARK, S.J., PARKS, K., SCHATZLEY, B.L., SCHOENBERGER, O.L., 
TASHIRO, M., VICHOT, G., WHATLEY, H.E., WIECZOREK, A. AND WIECZOREK, M.
Ovomucoid third domains from 100 avian species: isolation, sequences, and
hypervariability of enzyme-inhibitor contact residues.
BIOCHEMISTRY 26 202-221 (1987).
 
3. EMPIE, M.W. AND LASKOWSKI, M.
Thermodynamics and kinetics of single residue replacements in avian
ovomucoid third domains: effect on inhibitor interactions with serine 
proteinases.
BIOCHEMISTRY 21 2274-2284 (1982).

Documentation
Kazal inhibitors, which inhibit a number of serine proteases (such as
trypsin and elastase), belong to family of proteins that includes
pancreatic secretory trypsin inhibitor; avian ovomucoid; acrosin inhibitor;
and elastase inhibitor. These proteins contain between 1 and 7 Kazal-type
inhibitor repeats [1,2]. 
 
The structure of the Kazal repeat includes a large quantity of extended
chain, 2 short alpha-helices and a 3-stranded anti-parallel beta sheet [1].
The inhibitor makes 11 contacts with its enzyme substrate: unusually, 8 of
these important residues are hypervariable [2]. Altering the enzyme-contact
residues, and especially that of the active site bond, affects the the
strength of inhibition and specificity of the inhibitor for particular
serine proteases [2,3].
 
KAZALINHBTR is a 2-element fingerprint that provides a signature for Kazal-
type serine protease inhibitors. The fingerprint was derived from an
initial alignment of 10 sequences: the motifs were drawn from conserved
regions towards the N-terminus of the alignment and span the region encoded
by PROSITE pattern KAZAL (PS00282), which includes 4 Cys residues involved
in disulphide bond formation. Two iterations on OWL25.1 were required to
reach convergence, at which point a true set comprising 111 sequences was
identified.
 
An update on SPTR37_9f identified a true set of 45 sequences.
Summary Information
45 codes involving  2 elements
Composite Feature Index
24545
12
True Positives
AGRI_CHICK    AGRI_RAT      FRP_HUMAN     FRP_MOUSE     
FRP_RAT IAC1_BOVIN IAC2_BOVIN IAC2_HUMAN
IACA_PIG IACS_PIG IAC_MACFA IELA_ANESU
IOV7_CHICK IOVO_CHICK IOVO_COTJA IOVO_MELGA
IPS1_RAT IPS2_RAT IPSG_CANFA IPSG_FELCA
IPSG_MARMT IPSG_MELME IPSG_MUSLU IPSG_PANLE
IPSG_UNCUN IPSG_VULVU IPST_ANGAN IPST_BOVIN
IPST_CANFA IPST_HUMAN IPST_PIG IPST_SHEEP
LDTI_HIRME O13274 O75770 O77037
O77038 PE60_PIG PSG1_MOUSE Q13086
Q22452 Q26057 Q95011 QR1_COTJA
THBI_RHOPR
Sequence Titles
AGRI_CHICK  AGRIN PRECURSOR - GALLUS GALLUS (CHICKEN).    
AGRI_RAT AGRIN PRECURSOR - RATTUS NORVEGICUS (RAT).
FRP_HUMAN FOLLISTATIN-RELATED PROTEIN PRECURSOR - HOMO SAPIENS (HUMAN).
FRP_MOUSE FOLLISTATIN-RELATED PROTEIN PRECURSOR (TGF-BETA-INDUCIBLE PROTEIN TSC-36) - MUS MUSCULUS (MOUSE).
FRP_RAT FOLLISTATIN-RELATED PROTEIN PRECURSOR - RATTUS NORVEGICUS (RAT).
IAC1_BOVIN ACROSIN INHIBITOR I (BUSI-I) - BOS TAURUS (BOVINE).
IAC2_BOVIN ACROSIN INHIBITORS IIA AND IIB (BUSI-II) - BOS TAURUS (BOVINE).
IAC2_HUMAN ACROSIN-TRYPSIN INHIBITOR II PRECURSOR (HUSI-II) - HOMO SAPIENS (HUMAN).
IACA_PIG SEMINAL PLASMA ACROSIN INHIBITOR A1 - SUS SCROFA (PIG).
IACS_PIG SPERM-ASSOCIATED ACROSIN INHIBITOR PRECURSOR - SUS SCROFA (PIG).
IAC_MACFA ACROSIN-TRYPSIN INHIBITOR PRECURSOR - MACACA FASCICULARIS (CRAB EATING MACAQUE) (CYNOMOLGUS MONKEY).
IELA_ANESU ELASTASE INHIBITOR - ANEMONIA SULCATA (SNAKE-LOCKS SEA ANEMONE).
IOV7_CHICK OVOINHIBITOR PRECURSOR - GALLUS GALLUS (CHICKEN).
IOVO_CHICK OVOMUCOID PRECURSOR (ALLERGEN GAL D 1) (GAL D I) - GALLUS GALLUS (CHICKEN).
IOVO_COTJA OVOMUCOID - COTURNIX COTURNIX JAPONICA (JAPANESE QUAIL).
IOVO_MELGA OVOMUCOID - MELEAGRIS GALLOPAVO (COMMON TURKEY), AND AGRIOCHARIS OCELLATA (OCELLATED TURKEY).
IPS1_RAT PANCREATIC SECRETORY TRYPSIN INHIBITOR I PRECURSOR (PSTI-I) (CHOLECYSTOKININ-RELEASING PEPTIDE) (MONITOR PEPTIDE) - RATTUS NORVEGICUS (RAT).
IPS2_RAT PANCREATIC SECRETORY TRYPSIN INHIBITOR II PRECURSOR (PSTI-II) (CALTRIN) (CALCIUM TRANSPORT INHIBITOR) - RATTUS NORVEGICUS (RAT).
IPSG_CANFA DOUBLE-HEADED PROTEASE INHIBITOR, SUBMANDIBULAR GLAND - CANIS FAMILIARIS (DOG).
IPSG_FELCA DOUBLE-HEADED PROTEASE INHIBITOR, SUBMANDIBULAR GLAND - FELIS SILVESTRIS CATUS (CAT).
IPSG_MARMT DOUBLE-HEADED PROTEASE INHIBITOR, SUBMANDIBULAR GLAND - MARTES MARTES (EUROPEAN PINE MARTEN).
IPSG_MELME DOUBLE-HEADED PROTEASE INHIBITOR, SUBMANDIBULAR GLAND - MELES MELES (EURASIAN BADGER).
IPSG_MUSLU DOUBLE-HEADED PROTEASE INHIBITOR, SUBMANDIBULAR GLAND - MUSTELA LUTREOLA (EUROPEAN MINK).
IPSG_PANLE DOUBLE-HEADED PROTEASE INHIBITOR, SUBMANDIBULAR GLAND - PANTHERA LEO (LION).
IPSG_UNCUN DOUBLE-HEADED PROTEASE INHIBITOR, SUBMANDIBULAR GLAND - UNCIA UNCIA (SNOW LEOPARD) (PANTHERA UNCIA).
IPSG_VULVU DOUBLE-HEADED PROTEASE INHIBITOR, SUBMANDIBULAR GLAND - VULPES VULPES (RED FOX).
IPST_ANGAN PROBABLE PANCREATIC PROTEINASE INHIBITOR (PSTI TYPE) - ANGUILLA ANGUILLA (EUROPEAN FRESHWATER EEL).
IPST_BOVIN PANCREATIC SECRETORY TRYPSIN INHIBITOR - BOS TAURUS (BOVINE).
IPST_CANFA PANCREATIC SECRETORY TRYPSIN INHIBITOR - CANIS FAMILIARIS (DOG).
IPST_HUMAN PANCREATIC SECRETORY TRYPSIN INHIBITOR PRECURSOR (TUMOR-ASSOCIATED TRYPSIN INHIBITOR) (TATI) - HOMO SAPIENS (HUMAN).
IPST_PIG PANCREATIC SECRETORY TRYPSIN INHIBITOR - SUS SCROFA (PIG).
IPST_SHEEP PANCREATIC SECRETORY TRYPSIN INHIBITOR - OVIS ARIES (SHEEP).
LDTI_HIRME LEECH-DERIVED TRYPTASE INHIBITOR (LDTI) - HIRUDO MEDICINALIS (MEDICINAL LEECH).
O13274 SPERM-ACTIVATING PROTEIN - CLUPEA PALLASII (PACIFIC HERRING).
O75770 VAKTI PRECURSOR - HOMO SAPIENS (HUMAN).
O77037 P-SERPIN 1 - POLYANDROCARPA MISAKIENSIS.
O77038 P-SERPIN 2 - POLYANDROCARPA MISAKIENSIS.
PE60_PIG PEPTIDE PEC-60 PRECURSOR - SUS SCROFA (PIG).
PSG1_MOUSE PROSTATIC SECRETORY GLYCOPROTEIN PRECURSOR (P12) - MUS MUSCULUS (MOUSE).
Q13086 TRANSMEMBRANE PROTEIN PRECURSOR - HOMO SAPIENS (HUMAN).
Q22452 SIMILAR TO AGRIN AND FOLLISTATIN - CAENORHABDITIS ELEGANS.
Q26057 PROTEINASE INHIBITOR I (PAPI I) - PACIFASTACUS LENIUSCULUS (SIGNAL CRAYFISH).
Q95011 F29G6.1 PROTEIN - CAENORHABDITIS ELEGANS.
QR1_COTJA QR1 PROTEIN PRECURSOR - COTURNIX COTURNIX JAPONICA (JAPANESE QUAIL).
THBI_RHOPR RHODNIIN (THROMBIN INHIBITOR) - RHODNIUS PROLIXUS.
Scan History
OWL25_1    2  150  NSINGLE    
SPTR37_9f 4 300 NSINGLE
Initial Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
CTMEYRPVCGS IOVO_FRAAF 13 13 -
CTRESNPHCGS NRL_1BUS 17 17 -
CPLDYMPLCGS IOVO_STRCA 14 14 -
CSPEYMPLCGS IOVO_CASCA 14 14 -
CPRIYDPVCGT PSG1_MOUSE 40 40 -
CPKIYNPVCGT IPST_PIG 16 16 -
CPRLHQPICGT IPSG_CANFA 24 24 -
CPKDYRPVCGS IOVO_COTJA 146 146 -
CPRHFNPVCGS IAC2_HUMAN 44 44 -
CTREYNPICDS IAC1_BOVIN 21 21 -

Motif 2 width=12
Element Seqn Id St Int Rpt
DHKTYSNECMFC IPSG_CANFA 35 0 -
DNKTYSNKCNFC IOVO_COTJA 157 0 -
DMSTYANECTLC IAC2_HUMAN 55 0 -
AAKTYSNECTFC IAC1_BOVIN 32 0 -
DNITYGNKCNFC IOVO_FRAAF 24 0 -
NGETYGNKCAFC NRL_1BUS 28 0 -
DSKTYSNKCNFC IOVO_STRCA 25 0 -
DSKTYNNKCDFC IOVO_CASCA 25 0 -
DGITYANECVLC PSG1_MOUSE 51 0 -
DGITYSNECVLC IPST_PIG 27 0 -
Final Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
CPRDYDPVCGT IPS2_RAT 39 39 -
CPRIYNPVCGT IPST_BOVIN 16 16 -
CPRIYNPVCGT IPST_SHEEP 16 16 -
CPRIYDPVCGT PSG1_MOUSE 40 40 -
CTKIYDPVCGT IPST_HUMAN 39 39 -
CPKIYNPVCGT IPST_PIG 16 16 -
CTMEYLPLCGS IPSG_UNCUN 79 79 -
CTMEYLPLCGS IPSG_MARMT 79 79 -
CTMDYRPLCGS IPSG_VULVU 75 75 -
CPRLHQPICGT IPSG_CANFA 24 24 -
CPKDYRPVCGS IOVO_COTJA 146 146 -
CTMDYLPLCGS IPSG_MUSLU 79 79 -
CTMEYFPLCGS IPSG_PANLE 71 71 -
CTMEYVPHCGS IOV7_CHICK 432 432 -
CNKIYNPICGS IPST_CANFA 17 17 -
CTLEYRPLCGS IOVO_MELGA 145 145 -
CTMDYLPLCGS IPSG_MELME 79 79 -
CTMEYFPLCGS IPSG_FELCA 71 71 -
CPLNYKPVCGS Q26057 27 27 -
CTMQYDPVCGS IELA_ANESU 8 8 -
CTKEYRPICGS O13274 38 38 -
CSGIYDPVCGS AGRI_RAT 389 389 -
CPMNFAPVCGT IPST_ANGAN 21 21 -
CSRIYDPVCGT PE60_PIG 46 46 -
CTAEDRPLCGS IOVO_CHICK 170 170 -
CPRHFNPVCGS IAC2_HUMAN 44 44 -
CPRIYDPVCGT IPS1_RAT 39 39 -
CPRDFNPVCGT IAC_MACFA 41 41 -
CTRESNPHCGS IAC2_BOVIN 17 17 -
CQGRYDPVCGS AGRI_CHICK 378 378 -
CTREYNPICDS IAC1_BOVIN 21 21 -
CHTNYIPVCGS Q13086 103 103 -
CTMNSAHVCGT Q22452 322 322 -
CTREMDPICGT IACS_PIG 48 48 -
CPKILKPVCGS LDTI_HIRME 6 6 -
CKPHKRPVCGS FRP_HUMAN 58 58 -
CKPHKRPVCGS FRP_RAT 56 56 -
CKPHKRPVCGS FRP_MOUSE 56 56 -
CTRQMDPICGT IACA_PIG 17 17 -
CTRESDPVRGP O75770 239 239 -
CPSVIRPVCGT Q95011 21 21 -
DGDEYKPVCGS THBI_RHOPR 61 61 -
CTMEYDPICFV O77038 40 40 -
CTMEYDPICFV O77037 40 40 -
STKDYKRVCGT QR1_COTJA 476 476 -

Motif 2 width=12
Element Seqn Id St Int Rpt
DGKTYANECILC IPS2_RAT 50 0 -
DGVTYSNECLLC IPST_BOVIN 27 0 -
DGVTYANECLLC IPST_SHEEP 27 0 -
DGITYANECVLC PSG1_MOUSE 51 0 -
DGNTYPNECVLC IPST_HUMAN 50 0 -
DGITYSNECVLC IPST_PIG 27 0 -
DGKNYSNKCLFC IPSG_UNCUN 90 0 -
DGKNYSNKCLFC IPSG_MARMT 90 0 -
DGKNYSNKCIFC IPSG_VULVU 86 0 -
DHKTYSNECMFC IPSG_CANFA 35 0 -
DNKTYSNKCNFC IOVO_COTJA 157 0 -
DGKNYSNKCLFC IPSG_MUSLU 90 0 -
DGKVYSNKCSFC IPSG_PANLE 82 0 -
DGVTYSNRCFFC IOV7_CHICK 443 0 -
DGITYANECLLC IPST_CANFA 28 0 -
DNKTYGNKCNFC IOVO_MELGA 156 0 -
DGNNYSNKCLFC IPSG_MELME 90 0 -
DGQEYSNKCLFC IPSG_FELCA 82 0 -
DGKTYSNLCALR Q26057 89 51 -
DGITYGNACMLL IELA_ANESU 19 0 -
DDVTYENECLFC O13274 49 0 -
DGVTYGNECQLK AGRI_RAT 835 435 -
DGNTYPNECSLC IPST_ANGAN 32 0 -
DGVTYESECKLC PE60_PIG 57 0 -
DNKTYGNKCNFC IOVO_CHICK 181 0 -
DMSTYANECTLC IAC2_HUMAN 55 0 -
NGITYPSECSLC IPS1_RAT 50 0 -
DMITYPNECTLC IAC_MACFA 52 0 -
NGETYGNKCAFC IAC2_BOVIN 28 0 -
DDVTYANECELK AGRI_CHICK 604 215 -
AAKTYSNECTFC IAC1_BOVIN 32 0 -
NGDTYQNECFLR Q13086 114 0 -
DGKTYLNECFLK Q22452 333 0 -
NGKSYANPCIFC IACS_PIG 59 0 -
DGRTYANSCIAR LDTI_HIRME 17 0 -
NGKTYLNHCELH FRP_HUMAN 69 0 -
NGKTYLNHCELH FRP_RAT 67 0 -
NGKTYLNHCELH FRP_MOUSE 67 0 -
NGKSYANPCIFC IACA_PIG 28 0 -
DGKTHGNKCAMC O75770 657 407 -
DNVTYNNLCFLR Q95011 32 0 -
DDITYDNNCRLE THBI_RHOPR 72 0 -
ERKTASNKCFAC O77038 109 58 -
ERKTASNKCAAC O77037 111 60 -
DNKTYDGTCQLF QR1_COTJA 487 0 -