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PR00274

Identifier
GLYCOHORMONE  [View Relations]  [View Alignment]  
Accession
PR00274
No. of Motifs
4
Creation Date
15-DEC-1993  (UPDATE 07-JUN-1999)
Title
Glycoprotein hormone alpha chain signature
Database References

PROSITE; PS00779 GLYCO_HORMONE_ALPHA_1; PS00780 GLYCO_HORMONE_ALPHA_2
BLOCKS; BL00779
PFAM; PF00236 hormone6
INTERPRO; IPR000476
PDB; 1HCN
SCOP; 1HCN
Literature References
1. GOODWIN, R.G., MONCMAN, C.L., ROTTMAN, F.M. AND NILSON, J.H.
Characterization and nucleotide sequence of the gene for the common alpha
subunit of the bovine pituitary glycoprotein hormones.
NUCLEIC ACIDS RES. 11(19) 6873-6882 (1983).
 
2. GODINE, J.E., CHIN, W.W. AND HABENER, J.F.
Alpha subunit of rat pituitary glycoprotein hormones - Primary structure of
the precursor determined from the nucleotide sequence of cloned cDNAs.
J.BIOL.CHEM. 257(14) 8368-8371 (1982).
 
3. GOLOS, T.G., DURNING, M. AND FISHER, J.M.
Molecular cloning of the Rhesus glycoprotein hormone alpha subunit gene.
DNA CELL BIOL. 10(5) 367-380 (1991).

Documentation
The glycoprotein hormone family includes luteinising hormone (LH), follicle
stimulating hormone (FSH), thyroid stimulating hormone (TSH), and placental 
chorionic gonadotrophins hCG and eCG [1], all of which are central to the 
complex endocrine system that regulates normal growth, sexual development, 
and reproductive function [2]. The hormones LH, FSH and TSH are secreted
by the anterior pituitary gland, while hCG and eCG are secreted by the 
placenta [3]. 
 
The glycoprotein hormones are dimeric proteins, each containing a common
alpha subunit (well conserved within species, but differing between them
[2]), and a unique beta subunit, which confers biological specificity [1].
Intracellular levels of free alpha subunits are greater than those of the
mature glycoprotein, implying that hormone assembly is limited by the
appearance of the specific beta subunits, and hence that synthesis of alpha
and beta is independently regulated [1].
 
GLYCOHORMONE is a 4-element fingerprint that provides a signature for
the glycoprotein hormones. The fingerprint was derived from an initial 
alignment of 8 sequences: the motifs were drawn from conserved regions
spanning virtually the full alignment length, motifs 2 and 4 including
regions encoded by PROSITE patterns GLYCO_HORMONE_ALPHA_1 (PS00779) and
GLYCO_HORMONE_ALPHA_2 (PS00780). Two iterations on OWL22.1 were required
to reach convergence, at which point a true set comprising 23 sequences
was identified.
 
An update on SPTR37_9f identified a true set of 34 sequences.
Summary Information
34 codes involving  4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
434343434
30000
20000
1234
True Positives
GLH1_CYPCA    GLH1_RAT      GLH2_CYPCA    GLH2_ONCKE    
GLH2_RAT GLHA_ACALA GLHA_ANGAN GLHA_BALAC
GLHA_BOVIN GLHA_CALJA GLHA_CLAGA GLHA_CTEID
GLHA_EQUAS GLHA_FUNHE GLHA_HORSE GLHA_HUMAN
GLHA_HYPMO GLHA_MACMU GLHA_MACRU GLHA_MELGA
GLHA_MORSA GLHA_MOUSE GLHA_MURCI GLHA_PHYCA
GLHA_PIG GLHA_RABIT GLHA_RANCA GLHA_SHEEP
GLHA_STRCA GLHA_THUOB O46642 O77752
Q91370 Q91371
Sequence Titles
GLH1_CYPCA  GLYCOPROTEIN HORMONES ALPHA CHAIN 1 PRECURSOR (GONADOTROPIN ALPHA CHAIN 1) (GTH-ALPHA) - CYPRINUS CARPIO (COMMON CARP). 
GLH1_RAT GLYCOPROTEIN HORMONES ALPHA CHAIN 1 PRECURSOR - RATTUS NORVEGICUS (RAT).
GLH2_CYPCA GLYCOPROTEIN HORMONES ALPHA CHAIN 2 PRECURSOR (GONADOTROPIN ALPHA CHAIN 2) (GTH-ALPHA) - CYPRINUS CARPIO (COMMON CARP).
GLH2_ONCKE GLYCOPROTEIN HORMONES ALPHA CHAIN 2 PRECURSOR (GONADOTROPIN 2 ALPHA CHAIN) (GTH-ALPHA) - ONCORHYNCHUS KETA (CHUM SALMON), AND ONCORHYNCHUS TSCHAWYTSCHA (CHINOOK SALMON) (KING SALMON).
GLH2_RAT GLYCOPROTEIN HORMONES ALPHA CHAIN 2 PRECURSOR - RATTUS NORVEGICUS (RAT).
GLHA_ACALA GLYCOPROTEIN HORMONES ALPHA CHAIN PRECURSOR (GONADOTROPIN ALPHA CHAIN) (GTH-ALPHA) - ACANTHOPAGRUS LATUS (YELLOWFIN PORGY).
GLHA_ANGAN GLYCOPROTEIN HORMONES ALPHA CHAIN PRECURSOR (GONADOTROPIN ALPHA CHAIN) (GTH-ALPHA) - ANGUILLA ANGUILLA (EUROPEAN FRESHWATER EEL).
GLHA_BALAC GLYCOPROTEIN HORMONES ALPHA CHAIN - BALAENOPTERA ACUTOROSTRATA (MINKE WHALE) (LESSER RORQUAL).
GLHA_BOVIN GLYCOPROTEIN HORMONES ALPHA CHAIN PRECURSOR - BOS TAURUS (BOVINE).
GLHA_CALJA GLYCOPROTEIN HORMONES ALPHA CHAIN PRECURSOR - CALLITHRIX JACCHUS (COMMON MARMOSET).
GLHA_CLAGA GLYCOPROTEIN HORMONES ALPHA CHAIN PRECURSOR (GONADOTROPIN ALPHA CHAIN) (GTH-ALPHA) - CLARIAS GARIEPINUS (SHARPTOOTH CATFISH) (AFRICAN CATFISH).
GLHA_CTEID GLYCOPROTEIN HORMONES ALPHA CHAIN PRECURSOR (GONADOTROPIN ALPHA CHAIN) (GTH-ALPHA) - CTENOPHARYNGODON IDELLA (GRASS CARP).
GLHA_EQUAS GLYCOPROTEIN HORMONES ALPHA CHAIN PRECURSOR - EQUUS ASINUS (DONKEY).
GLHA_FUNHE GLYCOPROTEIN HORMONES ALPHA CHAIN PRECURSOR (GONADOTROPIN ALPHA CHAIN) (GTH-ALPHA) - FUNDULUS HETEROCLITUS (KILLIFISH) (MUMMICHOG).
GLHA_HORSE GLYCOPROTEIN HORMONES ALPHA CHAIN PRECURSOR - EQUUS CABALLUS (HORSE).
GLHA_HUMAN GLYCOPROTEIN HORMONES ALPHA CHAIN PRECURSOR - HOMO SAPIENS (HUMAN).
GLHA_HYPMO GLYCOPROTEIN HORMONES ALPHA CHAIN PRECURSOR (GONADOTROPIN ALPHA CHAIN) (GTH-ALPHA) - HYPOPHTHALMICHTHYS MOLITRIX (SILVER CARP).
GLHA_MACMU GLYCOPROTEIN HORMONES ALPHA CHAIN PRECURSOR - MACACA MULATTA (RHESUS MACAQUE).
GLHA_MACRU GLYCOPROTEIN HORMONES ALPHA CHAIN PRECURSOR - MACROPUS RUFUS (RED KANGAROO) (MEGALEIA RUFA), AND TRICHOSURUS VULPECULA (BRUSH-TAILED POSSUM).
GLHA_MELGA GLYCOPROTEIN HORMONES ALPHA CHAIN PRECURSOR - MELEAGRIS GALLOPAVO (COMMON TURKEY), AND COTURNIX COTURNIX JAPONICA (JAPANESE QUAIL).
GLHA_MORSA GLYCOPROTEIN HORMONES ALPHA CHAIN PRECURSOR (GONADOTROPIN ALPHA CHAIN) (GTH-ALPHA) - MORONE SAXATILIS (STRIPED BASS).
GLHA_MOUSE GLYCOPROTEIN HORMONES ALPHA CHAIN PRECURSOR - MUS MUSCULUS (MOUSE).
GLHA_MURCI GLYCOPROTEIN HORMONES ALPHA CHAIN (GONADOTROPIN ALPHA CHAIN) (GTH- ALPHA) (GTH-ALPHA) - MURAENESOX CINEREUS (PIKE EEL) (DAGGERTOOTH PIKE CONGER).
GLHA_PHYCA GLYCOPROTEIN HORMONES ALPHA CHAIN - PHYSETER CATODON (SPERM WHALE) (PHYSETER MACROCEPHALUS).
GLHA_PIG GLYCOPROTEIN HORMONES ALPHA CHAIN PRECURSOR - SUS SCROFA (PIG).
GLHA_RABIT GLYCOPROTEIN HORMONES ALPHA CHAIN - ORYCTOLAGUS CUNICULUS (RABBIT).
GLHA_RANCA GLYCOPROTEIN HORMONES ALPHA CHAIN - RANA CATESBEIANA (BULL FROG).
GLHA_SHEEP GLYCOPROTEIN HORMONES ALPHA CHAIN PRECURSOR - OVIS ARIES (SHEEP).
GLHA_STRCA GLYCOPROTEIN HORMONES ALPHA CHAIN - STRUTHIO CAMELUS (OSTRICH).
GLHA_THUOB GLYCOPROTEIN HORMONES ALPHA CHAIN (GONADOTROPIN ALPHA CHAIN) (GTH-ALPHA) - THUNNUS OBESUS (BIGEYE TUNA).
O46642 LUTEINIZING HORMONE ALPHA SUBUNIT PRECURSOR - EQUUS BURCHELLI (PLAINS ZEBRA) (EQUUS QUAGGA).
O77752 GONADOTROPHIN ALPHA SUBUNIT - TRICHOSURUS VULPECULA (BRUSH-TAILED POSSUM).
Q91370 GONADOTROPHIN ALPHA 1 SUBUNIT - ONCORHYNCHUS MASOU (CHERRY SALMON) (MASU SALMON).
Q91371 GONADOTROPHIN ALPHA 2 SUBUNIT - ONCORHYNCHUS MASOU (CHERRY SALMON) (MASU SALMON).
Scan History
OWL22_0    2  100  NSINGLE    
OWL26_0 1 100 NSINGLE
SPTR37_9f 2 35 NSINGLE
Initial Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
GCDECRLQENKIFSK GLHA_ANGAN 34 34 -
DCPECKPRENKFFSK GLHA_MACMU 34 34 -
GCEECKLKENKVFSN GLH1_ONCKE 23 23 -
DCPECTLQENPFFSQ GLHA_HUMAN 30 30 -
GCPECKLKENKYFSK GLH2_RAT 34 34 -
GCPECKLKENKYFSK GLHA_BOVIN 34 34 -
GCEECKLKENNIFSK GLH2_CYPCA 33 33 -
GCPZCKLKQNKYFSK GLHA_PHYCA 10 10 -

Motif 2 width=20
Element Seqn Id St Int Rpt
YQCMGCCFSRAYPTPVRSKK GLHA_MACMU 54 5 -
YQCTGCCFSRAYPTPLQSKK GLH1_ONCKE 43 5 -
YQCMGCCFSRAYPTPARSKK GLH2_RAT 54 5 -
YQCMGCCFSRAYPTPARSKK GLHA_BOVIN 54 5 -
YQCMGCCFSRAYPTPLRSKK GLH2_CYPCA 53 5 -
YZCMGCCFSRAYPTPARSKK GLHA_PHYCA 30 5 -
FQCVGCCFSRAYPTPLRSKK GLHA_ANGAN 54 5 -
LQCMGCCFSRAYPTPLRSKK GLHA_HUMAN 50 5 -

Motif 3 width=18
Element Seqn Id St Int Rpt
AMLVPKNITSEATCCVAK GLH1_ONCKE 63 0 -
TMLVPKNITSEATCCVAK GLH2_RAT 74 0 -
TMLVPKNITSEATCCVAK GLHA_BOVIN 74 0 -
TMLVPKNITSEATCCVAK GLH2_CYPCA 73 0 -
TMLVPKNITSZATCCVAK GLHA_PHYCA 50 0 -
TMLVPKNITSEATCCVAR GLHA_ANGAN 74 0 -
TMLVQKNVTSESTCCVAK GLHA_MACMU 74 0 -
TMLVQKNVTSESTCCVAK GLHA_HUMAN 70 0 -

Motif 4 width=17
Element Seqn Id St Int Rpt
RVENHTECHCSTCYYHK GLHA_MACMU 103 11 -
KVENHTACHCSTCYYHK GLHA_HUMAN 99 11 -
RVENHTECHCSTCYYHK GLH2_RAT 103 11 -
KLTNHTECWCNTCYHHK GLH1_ONCKE 91 10 -
RVENHTECHCSTCYYHK GLHA_BOVIN 103 11 -
KLVNHTDCHCSTCYYHK GLH2_CYPCA 101 10 -
RVQNHTZCHCSTCYYHK GLHA_PHYCA 79 11 -
KLENHTDCHCSTCYYHK GLHA_ANGAN 100 8 -
Final Motifs
Motif 1  width=15
Element Seqn Id St Int Rpt
GCPECKLKENKYFSK GLHA_MACRU 34 34 -
GCPECKLKENKYFSK GLHA_RABIT 10 10 -
GCPECKLKENKYFSK O77752 34 34 -
GCPECKLKENKYFSK GLH2_RAT 34 34 -
GCPECKLKENKYFSK GLHA_BOVIN 34 34 -
GCPECKLKENKYFSK GLHA_MOUSE 34 34 -
GCPECKLKENKYFSK GLHA_PIG 34 34 -
GCPECKLKENKYFSK GLHA_SHEEP 34 34 -
GCPECKLKENKYFSK GLH1_RAT 34 34 -
GCEECKLKENNIFSK GLH1_CYPCA 33 33 -
GCEECKLKENNIFSK GLH2_CYPCA 33 33 -
GCEECKLKENNIFSK GLHA_CLAGA 31 31 -
GCEECKLKENNIFSK GLHA_CTEID 33 33 -
GCEECKLKENNIFSK GLHA_HYPMO 33 33 -
GCPECKLGENRFFSK GLHA_STRCA 10 10 -
GCPECKLGENRFFSK GLHA_MELGA 34 34 -
DCPECKLKKNKYFSK GLHA_EQUAS 34 34 -
ECPECKLKENKYFSR GLHA_CALJA 34 34 -
DCPECKLKVNKYFSK O46642 34 34 -
GCPZCKLKQNKYFSK GLHA_PHYCA 10 10 -
GCDECRLQENKIFSK GLHA_ANGAN 34 34 -
GCPZCKLKZBKYFSK GLHA_BALAC 10 10 -
DCPECKPRENKFFSK GLHA_MACMU 34 34 -
GCDECRLKDNKFFSK GLHA_MURCI 10 10 -
DCPECKLRENKYFFK GLHA_HORSE 34 34 -
GCEECKLKENKLFSN Q91370 34 34 -
GCPECRLKENLRFSN GLHA_RANCA 10 10 -
GCEECTLKPNTIFPN GLH2_ONCKE 32 32 -
GCEECTLKPNTIFPN Q91371 32 32 -
DCPECTLQENPFFSQ GLHA_HUMAN 30 30 -
GCEECTLKKNNVFSR GLHA_THUOB 10 10 -
GCEECTLRKNSVFSR GLHA_MORSA 33 33 -
GCEACTLRKNTVFSR GLHA_ACALA 33 33 -
ACGECSLGLNRLFSR GLHA_FUNHE 40 40 -

Motif 2 width=20
Element Seqn Id St Int Rpt
YQCMGCCFSRAYPTPARSKK GLHA_MACRU 54 5 -
YQCMGCCFSRAYPTPARSKK GLHA_RABIT 30 5 -
YQCMGCCFSRAYPTPARSKK O77752 54 5 -
YQCMGCCFSRAYPTPARSKK GLH2_RAT 54 5 -
YQCMGCCFSRAYPTPARSKK GLHA_BOVIN 54 5 -
YQCMGCCFSRAYPTPARSKK GLHA_MOUSE 54 5 -
YQCMGCCFSRAYPTPARSKK GLHA_PIG 54 5 -
YQCMGCCFSRAYPTPARSKK GLHA_SHEEP 54 5 -
YQCMGCCFSRAYPTPARSKK GLH1_RAT 54 5 -
YQCMGCCFSRAYPTPLRSKK GLH1_CYPCA 53 5 -
YQCMGCCFSRAYPTPLRSKK GLH2_CYPCA 53 5 -
YQCMGCCFSRAYPTPLRSKK GLHA_CLAGA 51 5 -
YQCMGCCFSRAYPTPLRSKK GLHA_CTEID 53 5 -
YQCMGCCFSRAYPTPLRSKK GLHA_HYPMO 53 5 -
YQCTGCCFSRAYPTPLRSKK GLHA_STRCA 30 5 -
YQCTGCCFSRAYPTPMRSKK GLHA_MELGA 54 5 -
YQCMGCCFSRAYPTPARSKK GLHA_EQUAS 54 5 -
YQCMGCCFSRAYPTPLRSQK GLHA_CALJA 54 5 -
YQCMGCCFSRAYPTPARSRK O46642 54 5 -
YZCMGCCFSRAYPTPARSKK GLHA_PHYCA 30 5 -
FQCVGCCFSRAYPTPLRSKK GLHA_ANGAN 54 5 -
YZCMGCCFSRAYPTPARSKK GLHA_BALAC 30 5 -
YQCMGCCFSRAYPTPVRSKK GLHA_MACMU 54 5 -
FQCVGCCFSRAYPTPLRSKK GLHA_MURCI 30 5 -
YQCKGCCFSRAYPTPARSRK GLHA_HORSE 54 5 -
YQCTGCCFSRAYPTPLQSKK Q91370 54 5 -
YQCSGCCYSRAYPTPMRSKK GLHA_RANCA 31 6 -
MQCTGCCFSRAYPTPLRSKQ GLH2_ONCKE 48 1 -
IQCTGCCFSRAYPTPLRSKQ Q91371 48 1 -
LQCMGCCFSRAYPTPLRSKK GLHA_HUMAN 50 5 -
YQCMGCCFSRAFPTPLKAMK GLHA_THUOB 29 4 -
YQCMGCCFSRAYPTPLKAMK GLHA_MORSA 52 4 -
YQCMGCCFSRAYPTPLKAMK GLHA_ACALA 52 4 -
YQCMGCCFSRAYPTPQTAIQ GLHA_FUNHE 59 4 -

Motif 3 width=18
Element Seqn Id St Int Rpt
TMLVPKNITSEATCCVAK GLHA_MACRU 74 0 -
TMLVPKNITSEATCCVAK GLHA_RABIT 50 0 -
TMLVPKNITSEATCCVAK O77752 74 0 -
TMLVPKNITSEATCCVAK GLH2_RAT 74 0 -
TMLVPKNITSEATCCVAK GLHA_BOVIN 74 0 -
TMLVPKNITSEATCCVAK GLHA_MOUSE 74 0 -
TMLVPKNITSEATCCVAK GLHA_PIG 74 0 -
TMLVPKNITSEATCCVAK GLHA_SHEEP 74 0 -
TMLVPKNITSEATCCVAK GLH1_RAT 74 0 -
TMLVPKNITSEATCCVAK GLH1_CYPCA 73 0 -
TMLVPKNITSEATCCVAK GLH2_CYPCA 73 0 -
TMLVPKNITSEATCCVAK GLHA_CLAGA 71 0 -
TMLVPKNITSEATCCVAK GLHA_CTEID 73 0 -
TMLVPKNITSEATCCVAK GLHA_HYPMO 73 0 -
TMLVPKNITSEATCCVAK GLHA_STRCA 50 0 -
TMLVPKNITSEATCCVAK GLHA_MELGA 74 0 -
TMLVPKNITSEATCCVAK GLHA_EQUAS 74 0 -
TMLVPKNVTSESTCCVAK GLHA_CALJA 74 0 -
TMLVPKNITSEATCCVAK O46642 74 0 -
TMLVPKNITSZATCCVAK GLHA_PHYCA 50 0 -
TMLVPKNITSEATCCVAR GLHA_ANGAN 74 0 -
TMLVPKNITSZATCCVAK GLHA_BALAC 50 0 -
TMLVQKNVTSESTCCVAK GLHA_MACMU 74 0 -
TMLVPKDITSEATCCVAR GLHA_MURCI 50 0 -
TMLVPKNITSESTCCVAK GLHA_HORSE 74 0 -
AMLVPKNITSEATCCVAK Q91370 74 0 -
TMLVPKNITSEAKCCVAK GLHA_RANCA 51 0 -
TMLVPKNITSEATCCVAK GLH2_ONCKE 68 0 -
TMLVPKNITSEATCCVAK Q91371 68 0 -
TMLVQKNVTSESTCCVAK GLHA_HUMAN 70 0 -
TMTIPKNITSEATCCVAK GLHA_THUOB 49 0 -
TMTIPKNITSEATCCVAK GLHA_MORSA 72 0 -
TMTIPKNITSEATCCVAK GLHA_ACALA 72 0 -
TMAIPKNITSEAKCCVAK GLHA_FUNHE 79 0 -

Motif 4 width=17
Element Seqn Id St Int Rpt
KVENHTECHCSTCYYHK GLHA_MACRU 103 11 -
KVENHTECHCSTCYYHK GLHA_RABIT 79 11 -
KVENHTECHCSTCYYHK O77752 103 11 -
RVENHTECHCSTCYYHK GLH2_RAT 103 11 -
RVENHTECHCSTCYYHK GLHA_BOVIN 103 11 -
RVENHTECHCSTCYYHK GLHA_MOUSE 103 11 -
RVENHTECHCSTCYYHK GLHA_PIG 103 11 -
RVENHTECHCSTCYYHK GLHA_SHEEP 103 11 -
RVENHTDCHCSTCYYHK GLH1_RAT 103 11 -
KLVNHTDCHCSTCYYHK GLH1_CYPCA 101 10 -
KLVNHTDCHCSTCYYHK GLH2_CYPCA 101 10 -
KLVNHTDCHCSTCYYHK GLHA_CLAGA 99 10 -
KLVNHTDCHCSTCYYHK GLHA_CTEID 101 10 -
KLVNHTDCHCSTCYYHK GLHA_HYPMO 101 10 -
KIENHTECHCSTCYYHK GLHA_STRCA 79 11 -
KIENHTDCHCSTCYYHK GLHA_MELGA 103 11 -
RLENHTQCYCSTCYHHK GLHA_EQUAS 103 11 -
RVENHTECHCSTCYHHK GLHA_CALJA 103 11 -
RLENHTQCYCSTCYHHK O46642 103 11 -
RVQNHTZCHCSTCYYHK GLHA_PHYCA 79 11 -
KLENHTDCHCSTCYYHK GLHA_ANGAN 100 8 -
RVZNHTZCHCSTCYYHK GLHA_BALAC 79 11 -
RVENHTECHCSTCYYHK GLHA_MACMU 103 11 -
KLENHTDCHCSTCYYHK GLHA_MURCI 76 8 -
KLENHTQCYCSTCYHHK GLHA_HORSE 103 11 -
KLTNHTECWCNTCYHHK Q91370 102 10 -
KIENHTACHCSTCLYHK GLHA_RANCA 80 11 -
PVTNHTECHCSTCYYHK GLH2_ONCKE 97 11 -
PVTNHTECHCSTCYYHK Q91371 97 11 -
KVENHTACHCSTCYYHK GLHA_HUMAN 99 11 -
RVRNHTDCHCSTCYFHK GLHA_THUOB 77 10 -
KVRNHTDCHCSTCYFHK GLHA_MORSA 100 10 -
RVRNHTDCHCSTCYYHK GLHA_ACALA 100 10 -
TVRNHTECHCSTCYYHK GLHA_FUNHE 107 10 -