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PR00246

Identifier
SOMATOSTATNR  [View Relations]  [View Alignment]  
Accession
PR00246
No. of Motifs
7
Creation Date
15-JUL-1994  (UPDATE 07-JUN-1999)
Title
Somatostatin receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR00587 SOMATOSTTN1R; PR00588 SOMATOSTTN2R; PR00589 SOMATOSTTN3R
PRINTS; PR00590 SOMATOSTTN4R; PR00591 SOMATOSTTN5R
INTERPRO; IPR000586
GCRDB; GCR_0190
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. WATSON, S. AND ARKINSTALL, S.
Somatostatin.
IN THE G PROTEIN-LINKED RECEPTOR FACTSBOOK, ACADEMIC PRESS, PP.252-260.

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Somatostatin is a neurotransmitter/hormone with a wide range of biologial
functions [6]. It has an important role in the neuroendocrine system and
inhibits secretion of growth hormone and prolactin in the anterior
pituitary. It also inhibits secretion in the intestine (including gastric
acid in the stomach), pancreatic acinar cells and pancreatic beta-cells,
stimulates absorption in the intestine and modulates smooth muscle 
contractility. In the CNS, it is a neurotransmitter activating a hyper-
polarising K+ current and inhibiting Ca2+ influx, and is believed to
play important roles in regulating locomotor activity and cognitive
function. Receptor subtypes were originally proposed on the basis of
functional and radio-ligand binding studies, and cloning has now confirmed
the presence of at least 4 subtypes. 
 
SOMATOSTATNR is a 7-element fingerprint that provides a signature for the
somatostatin receptors. The fingerprint was derived from an initial
alignment of 8 sequences: the motifs were drawn from conserved sections
within either loop or TM regions, focusing on those areas of the alignment
that characterise the somatostatin receptors but distinguish them from the
rest of the rhodopsin-like superfamily - motif 1 lies at the N-terminus
leading into TM domain 1; motif 2 lies in the first external loop; motif 3
lies at the N-terminus of TM domain 3; motifs 4 and 5 lie in the third
cytoplasmic loop; motif 6 spans the C-terminus of TM domain 6 and part of
the third external loop; and motif 7 lies at the C-terminus. Two iterations
on OWL23.2 were required to reach convergence, at which point a true set
comprising 20 sequences was identfied. Several partial matches were also
found, all of which are opioid receptors.
 
An update on SPTR37_9f identified a true set of 17 sequences.
Summary Information
17 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
717171717171717
60000000
50000000
40000000
30000000
20000000
1234567
True Positives
SSR1_HUMAN    SSR1_MOUSE    SSR1_RAT      SSR2_BOVIN    
SSR2_HUMAN SSR2_MOUSE SSR2_PIG SSR2_RAT
SSR3_HUMAN SSR3_MOUSE SSR3_RAT SSR4_HUMAN
SSR4_MOUSE SSR4_RAT SSR5_HUMAN SSR5_MOUSE
SSR5_RAT
Sequence Titles
SSR1_HUMAN  Somatostatin receptor type 1 (SS1R) (SRIF-2) - Homo sapiens (Human). 
SSR1_MOUSE Somatostatin receptor type 1 (SS1R) (SRIF-2) - Mus musculus (Mouse).
SSR1_RAT Somatostatin receptor type 1 (SS1R) (SRIF-2) - Rattus norvegicus (Rat).
SSR2_BOVIN Somatostatin receptor type 2 (SS2R) (SRIF-1) - Bos taurus (Bovine).
SSR2_HUMAN Somatostatin receptor type 2 (SS2R) (SRIF-1) - Homo sapiens (Human).
SSR2_MOUSE Somatostatin receptor type 2 (SS2R) (SRIF-1) - Mus musculus (Mouse).
SSR2_PIG Somatostatin receptor type 2 (SS2R) (SRIF-1) - Sus scrofa (Pig).
SSR2_RAT Somatostatin receptor type 2 (SS2R) (SRIF-1) - Rattus norvegicus (Rat).
SSR3_HUMAN Somatostatin receptor type 3 (SS3R) (SSR-28) - Homo sapiens (Human).
SSR3_MOUSE Somatostatin receptor type 3 (SS3R) (SSR-28) - Mus musculus (Mouse).
SSR3_RAT Somatostatin receptor type 3 (SS3R) (SSR-28) - Rattus norvegicus (Rat).
SSR4_HUMAN Somatostatin receptor type 4 (SS4R) - Homo sapiens (Human).
SSR4_MOUSE Somatostatin receptor type 4 (SS4R) - Mus musculus (Mouse).
SSR4_RAT Somatostatin receptor type 4 (SS4R) - Rattus norvegicus (Rat).
SSR5_HUMAN Somatostatin receptor type 5 (SS5R) - Homo sapiens (Human).
SSR5_MOUSE Somatostatin receptor type 5 (SS5R) - Mus musculus (Mouse).
SSR5_RAT Somatostatin receptor type 5 (SS5R) - Rattus norvegicus (Rat).
Scan History
OWL23_2    2  100  NSINGLE    
OWL28_0 1 150 NSINGLE
SPTR37_9f 2 43 NSINGLE
Initial Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
GMVAIQCIYALVC SSR4_HUMAN 46 46 -
SGVLIPLVYLVVC SSR3_HUMAN 43 43 -
SNVVLTFIYFVVC SSR2_BOVIN 41 41 -
SNAVLTFIYFVVC SSRB_MOUSE 42 42 -
SNAVLTFIYFVVC SSRA_MOUSE 42 42 -
RAVLVPVLYLLVC SSRX_HUMAN 79 79 -
SAILISFIYSVVC SSR1_HUMAN 57 57 -
RAVLVPVLYLLVC SSR5_RAT 36 36 -

Motif 2 width=9
Element Seqn Id St Int Rpt
PFLAMQVAL SSR2_BOVIN 96 42 -
PFLVTSTLL SSR1_HUMAN 112 42 -
PFVASSAAL SSR4_HUMAN 101 42 -
PFLAMQVAL SSRA_MOUSE 97 42 -
PFLAMQVAL SSRB_MOUSE 97 42 -
PFLATQNAR SSRX_HUMAN 136 44 -
PFLATQNAV SSR5_RAT 91 42 -
PFLAAQNAL SSR3_HUMAN 98 42 -

Motif 3 width=10
Element Seqn Id St Int Rpt
RVVMTVDGIN SSRB_MOUSE 116 10 -
RVVMTVDGIN SSRA_MOUSE 116 10 -
RLVMTLDGVN SSRX_HUMAN 154 9 -
RVVMTVDGIN SSR2_BOVIN 115 10 -
RLVMAVDGIN SSR3_HUMAN 117 10 -
RLVMTLDGIN SSR5_RAT 111 11 -
RAVLSVDGLN SSR4_HUMAN 120 10 -
RLVLSVDAVN SSR1_HUMAN 131 10 -

Motif 4 width=10
Element Seqn Id St Int Rpt
AKMRMVALKA SSR1_HUMAN 248 107 -
VKVRAAGVRV SSRX_HUMAN 266 102 -
IKVKSSGIRV SSRB_MOUSE 233 107 -
IKVKSSGIRV SSRA_MOUSE 233 107 -
IKVKSSGIRV SSR2_BOVIN 232 107 -
VKVRSAGRRV SSR3_HUMAN 231 104 -
VKVKAAGMRV SSR5_RAT 225 104 -
GKMRAVALRA SSR4_HUMAN 236 106 -

Motif 5 width=14
Element Seqn Id St Int Rpt
GWQQRRRSEKKITR SSR4_HUMAN 246 0 -
VGCVRRRSERKVTR SSRX_HUMAN 275 -1 -
GSSKRKKSEKKVTR SSRB_MOUSE 243 0 -
GSSKRKKSEKKVTR SSRA_MOUSE 243 0 -
GSSRRRRSEPKVTR SSR5_RAT 235 0 -
SCQRRRRSERRVTR SSR3_HUMAN 244 3 -
GSSKRKKSEKKVTR SSR2_BOVIN 242 0 -
GWQQRKRSERKITL SSR1_HUMAN 258 0 -

Motif 6 width=9
Element Seqn Id St Int Rpt
NVSSVSVAI SSRB_MOUSE 276 19 -
NVSSVSVAI SSR2_BOVIN 275 19 -
NIVNVVCPL SSR3_HUMAN 277 19 -
NIVNLAFTL SSR5_RAT 268 19 -
QLLNLVVTS SSR4_HUMAN 279 19 -
QLVNVFAEQ SSR1_HUMAN 291 19 -
NIVNLAVAL SSRX_HUMAN 308 19 -
NVSSVSVAI SSRA_MOUSE 276 19 -

Motif 7 width=13
Element Seqn Id St Int Rpt
FLSDNFKKSFQNV SSRB_MOUSE 314 29 -
FLSDNFKRSFQRI SSR1_HUMAN 325 25 -
FLSDNFRRSFQRV SSR4_HUMAN 313 25 -
FLSDNFRQSFRKV SSR5_RAT 306 29 -
FLSYRFKQGFRRV SSR3_HUMAN 315 29 -
FLSDNFKKSFQNV SSR2_BOVIN 313 29 -
FLSDNFRQSFQKV SSRX_HUMAN 346 29 -
FLSDNFKKSFQNV SSRA_MOUSE 314 29 -
Final Motifs
Motif 1  width=13
Element Seqn Id St Int Rpt
SNVVLTFIYFVVC SSR2_BOVIN 41 41 -
SNAVLTFIYFVVC SSR2_MOUSE 42 42 -
SNAVLTFIYFVVC SSR2_PIG 42 42 -
SNAVLTFIYFVVC SSR2_RAT 42 42 -
SNAVLTFIYFVVC SSR2_HUMAN 42 42 -
SGVLIPLVYLVVC SSR3_HUMAN 43 43 -
SGILISLVYLVVC SSR3_MOUSE 44 44 -
SGILISLVYLVVC SSR3_RAT 44 44 -
RAVLVPVLYLLVC SSR5_RAT 36 36 -
RAVLVPVLYLLVC SSR5_MOUSE 36 36 -
RAVLVPVLYLLVC SSR5_HUMAN 39 39 -
GMVTIQCIYALVC SSR4_RAT 42 42 -
GMVAIQCIYALVC SSR4_HUMAN 46 46 -
SAILISFIYSVVC SSR1_HUMAN 57 57 -
SAILISFIYSVVC SSR1_RAT 57 57 -
SAILISFIYSVVC SSR1_MOUSE 57 57 -
GMVTIQCIYALVC SSR4_MOUSE 42 42 -

Motif 2 width=9
Element Seqn Id St Int Rpt
PFLAMQVAL SSR2_BOVIN 96 42 -
PFLAMQVAL SSR2_MOUSE 97 42 -
PFLAMQVAL SSR2_PIG 97 42 -
PFLAMQVAL SSR2_RAT 97 42 -
PFLAMQVAL SSR2_HUMAN 97 42 -
PFLAAQNAL SSR3_HUMAN 98 42 -
PFLAAQNAL SSR3_MOUSE 99 42 -
PFLAAQNAL SSR3_RAT 99 42 -
PFLATQNAV SSR5_RAT 91 42 -
PFLATQNAV SSR5_MOUSE 91 42 -
PFLATQNAA SSR5_HUMAN 94 42 -
PFVASAAAL SSR4_RAT 97 42 -
PFVASSAAL SSR4_HUMAN 101 42 -
PFLVTSTLL SSR1_HUMAN 112 42 -
PFLVTSTLL SSR1_RAT 112 42 -
PFLVTSTLL SSR1_MOUSE 112 42 -
PFVRSAAAL SSR4_MOUSE 97 42 -

Motif 3 width=10
Element Seqn Id St Int Rpt
RVVMTVDGIN SSR2_BOVIN 115 10 -
RVVMTVDGIN SSR2_MOUSE 116 10 -
RVVMTVDGIN SSR2_PIG 116 10 -
RVVMTVDGIN SSR2_RAT 116 10 -
RVVMTVDGIN SSR2_HUMAN 116 10 -
RLVMAVDGIN SSR3_HUMAN 117 10 -
RLVMAVDGIN SSR3_MOUSE 118 10 -
RLVMAVDGIN SSR3_RAT 118 10 -
RLVMTLDGIN SSR5_RAT 111 11 -
RLVMTLDGIN SSR5_MOUSE 110 10 -
RLVMTLDGVN SSR5_HUMAN 113 10 -
RAVLSVDGLN SSR4_RAT 116 10 -
RAVLSVDGLN SSR4_HUMAN 120 10 -
RLVLSVDAVN SSR1_HUMAN 131 10 -
RLVLSVDAVN SSR1_RAT 131 10 -
RLVLSVDAVN SSR1_MOUSE 131 10 -
RAVLSVDGLN SSR4_MOUSE 116 10 -

Motif 4 width=10
Element Seqn Id St Int Rpt
IKVKSSGIRV SSR2_BOVIN 232 107 -
IKVKSSGIRV SSR2_MOUSE 233 107 -
IKVKSSGIRV SSR2_PIG 233 107 -
IKVKSSGIRV SSR2_RAT 233 107 -
IKVKSSGIRV SSR2_HUMAN 233 107 -
VKVRSAGRRV SSR3_HUMAN 231 104 -
VKVRSTTRRV SSR3_MOUSE 232 104 -
VKVRSTTRRV SSR3_RAT 232 104 -
VKVKAAGMRV SSR5_RAT 225 104 -
VKVKAAGMRV SSR5_MOUSE 224 104 -
VKVRAAGVRV SSR5_HUMAN 226 103 -
GKMRAVALRA SSR4_RAT 232 106 -
GKMRAVALRA SSR4_HUMAN 236 106 -
AKMRMVALKA SSR1_HUMAN 248 107 -
AKMRMVALKA SSR1_RAT 248 107 -
AKMRMVALKA SSR1_MOUSE 248 107 -
GKMRAVALRG SSR4_MOUSE 232 106 -

Motif 5 width=14
Element Seqn Id St Int Rpt
GSSKRKKSEKKVTR SSR2_BOVIN 242 0 -
GSSKRKKSEKKVTR SSR2_MOUSE 243 0 -
GSSKRKKSEKKVTR SSR2_PIG 243 0 -
GSSKRKKSEKKVTR SSR2_RAT 243 0 -
GSSKRKKSEKKVTR SSR2_HUMAN 243 0 -
SCQRRRRSERRVTR SSR3_HUMAN 244 3 -
ACQRRRRSERRVTR SSR3_MOUSE 253 11 -
ACQRRRRSERRVTR SSR3_RAT 253 11 -
GSSRRRRSEPKVTR SSR5_RAT 235 0 -
GSSRRRRSERKVTR SSR5_MOUSE 234 0 -
VGCVRRRSERKVTR SSR5_HUMAN 235 -1 -
GWQQRRRSEKKITR SSR4_RAT 242 0 -
GWQQRRRSEKKITR SSR4_HUMAN 246 0 -
GWQQRKRSERKITL SSR1_HUMAN 258 0 -
GWQQRKRSERKITL SSR1_RAT 258 0 -
GWQQRKRSERKITL SSR1_MOUSE 258 0 -
GWQQRRRSEKKITR SSR4_MOUSE 242 0 -

Motif 6 width=9
Element Seqn Id St Int Rpt
NVSSVSVAI SSR2_BOVIN 275 19 -
NVSSVSVAI SSR2_MOUSE 276 19 -
NVSSVSVAI SSR2_PIG 276 19 -
NVSSVSVAI SSR2_RAT 276 19 -
NVSSVSMAI SSR2_HUMAN 276 19 -
NIVNVVCPL SSR3_HUMAN 277 19 -
NIVNVVCPL SSR3_MOUSE 286 19 -
NIVNVVCPL SSR3_RAT 286 19 -
NIVNLAFTL SSR5_RAT 268 19 -
NIVNLAFTL SSR5_MOUSE 267 19 -
NIVNLAVAL SSR5_HUMAN 268 19 -
QLLNLFVTS SSR4_RAT 275 19 -
QLLNLVVTS SSR4_HUMAN 279 19 -
QLVNVFAEQ SSR1_HUMAN 291 19 -
QLVNVFAEQ SSR1_RAT 291 19 -
QLVNVFAEQ SSR1_MOUSE 291 19 -
QLLNLFVTS SSR4_MOUSE 275 19 -

Motif 7 width=13
Element Seqn Id St Int Rpt
FLSDNFKKSFQNV SSR2_BOVIN 313 29 -
FLSDNFKKSFQNV SSR2_MOUSE 314 29 -
FLSDNFKKSFQNV SSR2_PIG 314 29 -
FLSDNFKKSFQNV SSR2_RAT 314 29 -
FLSDNFKKSFQNV SSR2_HUMAN 314 29 -
FLSYRFKQGFRRV SSR3_HUMAN 315 29 -
FLSYRFKQGFRRI SSR3_MOUSE 324 29 -
FLSYRFKQGFRRI SSR3_RAT 324 29 -
FLSDNFRQSFRKV SSR5_RAT 306 29 -
FLSDNFRQSFRKA SSR5_MOUSE 305 29 -
FLSDNFRQSFQKV SSR5_HUMAN 306 29 -
FLSDNFRRSFQRV SSR4_RAT 309 25 -
FLSDNFRRSFQRV SSR4_HUMAN 313 25 -
FLSDNFKRSFQRI SSR1_HUMAN 325 25 -
FLSDNFKRSFQRI SSR1_RAT 325 25 -
FLSDNFKRSFQRI SSR1_MOUSE 325 25 -
FLSDNFRRSFQRV SSR4_MOUSE 309 25 -