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PR00244

Identifier
NEUROKININR  [View Relations]  [View Alignment]  
Accession
PR00244
No. of Motifs
8
Creation Date
14-JUL-1994  (UPDATE 03-JUL-1999)
Title
Neurokinin receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR01024 NEUROKININ1R; PR01025 NEUROKININ2R; PR01026 NEUROKININ3R

INTERPRO; IPR001681
GCRDB; GCR_0244; GCR_0258; GCR_0243; GCR_0081; GCR_0359; GCR_0385
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. YOKOTA, Y., SASAI, Y., TANAKA, K., FUJIWARA, T, TSUCHIDA, K., 
SHIGEMOTO, R., KAKIZUKA, A., OHKUBO, H. AND NAKANISHI, S.
Molecular characterisation of a functional cDNA for rat substance P
receptor.
J.BIOL.CHEM. 264(30) 17649-17652 (1989).

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Neuropeptide receptors are present in very small quantities in the cell
and are embedded tightly in the plasma membrane. The neuropeptides exhibit
a high degree of functional diversity through both regulation of peptide
production and through peptide-receptor interaction [6]. The mammalian
tachykinin system consists of 3 distinct peptides: substance P, substance
K and neuromedin K. All possess a common spectrum of biological activities,
including sensory transmission in the nervous system and contraction/
relaxation of peripheral smooth muscles, and each interacts with a 
specific receptor type.
 
NEUROKININR is an 8-element fingerprint that provides a signature for the
neurokinin receptors. The fingerprint was derived from an initial alignment
of 6 sequences: the motifs were drawn from conserved sections within either
loop or TM regions, focusing on those areas of the alignment that
characterise the neurokinin receptors but distinguish them from the
rest of the rhodopsin-like superfamily - motif 1 lies at the N-terminus
leading into TM domain 1; motif 2 spans the C-terminus of TM domain 1 and
part of the first cytoplasmic loop; motif 3 spans part of the first
external loop leading into TM domain 3; motif 4 lies in the second
cytoplasmic loop; motif 5 lies at the N-terminus of TM domain 5; motif 6
spans the C-terminus of TM domain 5 leading into the third cytoplasmic
loop; motif 7 spans part of the third external loop leading into TM domain
7; and motif 8 lies at the C-terminus. Two iterations on OWL23.2 were
required to reach convergence, at which point a true set comprising 16
sequences was identified. Several partial matches were also found, all
of which are neurokinin receptor fragments or close superfamily relatives.
 
An update on SPTR37_9f identified a true set of 15 sequences, and 5
partial matches.
Summary Information
  15 codes involving  8 elements
0 codes involving 7 elements
0 codes involving 6 elements
1 codes involving 5 elements
0 codes involving 4 elements
1 codes involving 3 elements
2 codes involving 2 elements
Composite Feature Index
81515151515151515
700000000
600000000
511000111
400000000
300000111
202000002
12345678
True Positives
NK1R_CAVPO    NK1R_HUMAN    NK1R_MOUSE    NK1R_RANCA    
NK1R_RAT NK2R_BOVIN NK2R_CAVPO NK2R_HUMAN
NK2R_MESAU NK2R_MOUSE NK2R_RABIT NK2R_RAT
NK3R_HUMAN NK3R_RAT NK4R_HUMAN
True Positive Partials
Codes involving 5 elements
Q94736
Codes involving 3 elements
TLR2_DROME
Codes involving 2 elements
O44148 YLD1_CAEEL
Sequence Titles
NK1R_CAVPO  Substance-P receptor (SPR) (NK-1 receptor) (NK-1R) (Tachykinin receptor 1) - Cavia porcellus (Guinea pig). 
NK1R_HUMAN Substance-P receptor (SPR) (NK-1 receptor) (NK-1R) (Tachykinin receptor 1) - Homo sapiens (Human).
NK1R_MOUSE Substance-P receptor (SPR) (NK-1 receptor) (NK-1R) (Tachykinin receptor 1) - Mus musculus (Mouse).
NK1R_RANCA Substance-P receptor (SPR) (NK-1 receptor) (NK-1R) (Tachykinin receptor 1) - Rana catesbeiana (Bull frog).
NK1R_RAT Substance-P receptor (SPR) (NK-1 receptor) (NK-1R) (Tachykinin receptor 1) - Rattus norvegicus (Rat).
NK2R_BOVIN Substance-K receptor (SKR) (Neurokinin A receptor) (NK-2 receptor) (NK-2R) (Tachykinin receptor 2) - Bos taurus (Bovine).
NK2R_CAVPO Substance-K receptor (SKR) (Neurokinin A receptor) (NK-2 receptor) (NK-2R) (Tachykinin receptor 2) - Cavia porcellus (Guinea pig).
NK2R_HUMAN Substance-K receptor (SKR) (Neurokinin A receptor) (NK-2 receptor) (NK-2R) (Tachykinin receptor 2) - Homo sapiens (Human).
NK2R_MESAU Substance-K receptor (SKR) (Neurokinin A receptor) (NK-2 receptor) (NK-2R) (Tachykinin receptor 2) - Mesocricetus auratus (Golden hamster).
NK2R_MOUSE Substance-K receptor (SKR) (Neurokinin A receptor) (NK-2 receptor) (NK-2R) (Tachykinin receptor 2) - Mus musculus (Mouse).
NK2R_RABIT Substance-K receptor (SKR) (Neurokinin A receptor) (NK-2 receptor) (NK-2R) (Tachykinin receptor 2) - Oryctolagus cuniculus (Rabbit).
NK2R_RAT Substance-K receptor (SKR) (Neurokinin A receptor) (NK-2 receptor) (NK-2R) (Tachykinin receptor 2) - Rattus norvegicus (Rat).
NK3R_HUMAN Neuromedin-K receptor (NKR) (Neurokinin B receptor) (NK-3 receptor) (NK-3R) (Tachykinin receptor 3) - Homo sapiens (Human).
NK3R_RAT Neuromedin-K receptor (NKR) (Neurokinin B receptor) (NK-3 receptor) (NK-3R) (Tachykinin receptor 3) - Rattus norvegicus (Rat).
NK4R_HUMAN NEUROMEDIN K RECEPTOR (NKR) (NEUROKININ B RECEPTOR) (NK-4 RECEPTOR) (NK-4R) (K1R) (NEUROKININ 4 RECEPTOR) (NK4) - HOMO SAPIENS (HUMAN).

Q94736 TACHYKININ-LIKE RECEPTOR - STOMOXYS CALCITRANS (STABLE FLY).

TLR2_DROME Tachykinin-like peptides receptor 99D (dTKR) - Drosophila melanogaster (Fruit fly).

O44148 C49A9.7 PROTEIN - CAENORHABDITIS ELEGANS.
YLD1_CAEEL Probable G-protein coupled receptor C38C10.1 - Caenorhabditis elegans.
Scan History
OWL30_2    4  100  NSINGLE    
SPTR37_9f 1 100 NSINGLE
Initial Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
PSWRIALWSLAY NK3R_RAT 68 68 -
PSWRIALWSLAY NK3R_HUMAN 81 81 -
PAWQIVLWAAAY NK1R_HUMAN 28 28 -
PAWQIVLWAAAY NK1R_CAVPO 28 28 -
PSWQLALWAPAY NK2R_HUMAN 29 29 -
PGWQLALWATAY NK2R_MOUSE 29 29 -

Motif 2 width=10
Element Seqn Id St Int Rpt
IWIILAHKRM NK3R_RAT 94 14 -
IWIILAHKRM NK3R_HUMAN 107 14 -
MWIILAHKRM NK1R_HUMAN 54 14 -
MWIILAHKRM NK1R_CAVPO 54 14 -
IWIILAHRRM NK2R_HUMAN 55 14 -
IWIILAHERM NK2R_MOUSE 55 14 -

Motif 3 width=11
Element Seqn Id St Int Rpt
FQNFFPITAVF NK3R_RAT 147 43 -
FQNFFPITAVF NK3R_HUMAN 160 43 -
FHNFFPIAAVF NK1R_HUMAN 107 43 -
FHNFFPIAAVF NK1R_CAVPO 107 43 -
FQNLFPITAMF NK2R_HUMAN 108 43 -
FQNLFPVTAMF NK2R_MOUSE 108 43 -

Motif 4 width=11
Element Seqn Id St Int Rpt
LKPRLSATATK NK3R_RAT 178 20 -
LKPRLSATATK NK3R_HUMAN 191 20 -
LQPRLSATATK NK1R_HUMAN 138 20 -
LQPRLSATATK NK1R_CAVPO 138 20 -
FQPRLSAPSTK NK2R_HUMAN 139 20 -
FQPRLSAPSTK NK2R_MOUSE 139 20 -

Motif 5 width=10
Element Seqn Id St Int Rpt
YHIIVIILVY NK3R_RAT 234 45 -
YHIIVIILVY NK3R_HUMAN 247 45 -
YHICVTVLIY NK1R_HUMAN 196 47 -
YHICVTVLIY NK1R_CAVPO 196 47 -
YHLVVIALIY NK2R_HUMAN 197 47 -
YHLVVFVLIY NK2R_MOUSE 197 47 -

Motif 6 width=14
Element Seqn Id St Int Rpt
TIVGITLWGGEIPG NK3R_RAT 255 11 -
TIVGITLWGGEIPG NK3R_HUMAN 268 11 -
TVVGITLWASEIPG NK1R_HUMAN 217 11 -
TVVGITLWASEIPG NK1R_CAVPO 217 11 -
SVIGLTLWRRAVPG NK2R_HUMAN 218 11 -
SVIGLTLWKRAVPR NK2R_MOUSE 218 11 -

Motif 7 width=12
Element Seqn Id St Int Rpt
WKYIQQVYLASF NK3R_RAT 318 49 -
WKYIQQVYLASF NK3R_HUMAN 331 49 -
KKFIQQVYLAIM NK1R_HUMAN 280 49 -
KKFIQQVYLAIM NK1R_CAVPO 280 49 -
HKFIQQVYLALF NK2R_HUMAN 282 50 -
RKFIQQVYLALF NK2R_MOUSE 282 50 -

Motif 8 width=19
Element Seqn Id St Int Rpt
CCLNKRFRAGFKRAFRWCP NK3R_RAT 344 14 -
CCLNKRFRAGFKRAFRWCP NK3R_HUMAN 357 14 -
CCLNDRFRLGFKHAFRCCP NK1R_HUMAN 306 14 -
CCLNDRFRLGFKHAFRCCP NK1R_CAVPO 306 14 -
CCLNHRFRSGFRLAFRCCP NK2R_HUMAN 308 14 -
CCLNHRFRSGFRLAFRCCP NK2R_MOUSE 308 14 -
Final Motifs
Motif 1  width=12
Element Seqn Id St Int Rpt
PSWRIALWSLAY NK3R_RAT 68 68 -
PSWRIALWSLAY NK3R_HUMAN 81 81 -
PTWQIVLWAAAY NK1R_RAT 28 28 -
PTWQIVLWAAAY NK1R_MOUSE 28 28 -
PAWQIVLWAAAY NK1R_HUMAN 28 28 -
PAWQIVLWAAAY NK1R_CAVPO 28 28 -
PSWQLALWAPAY NK2R_HUMAN 29 29 -
PGWQLALWATAY NK2R_RAT 29 29 -
PTWQLALWATAY NK2R_CAVPO 29 29 -
PAWQLALWATAY NK2R_MESAU 29 29 -
PGWQLALWATAY NK2R_MOUSE 29 29 -
PGWQLALWTAAY NK2R_BOVIN 29 29 -
PPWAVALWSLAY NK4R_HUMAN 56 56 -
PGWQLALWATAY NK2R_RABIT 29 29 -
PPWQIALWSVAY NK1R_RANCA 29 29 -

Motif 2 width=10
Element Seqn Id St Int Rpt
IWIILAHKRM NK3R_RAT 94 14 -
IWIILAHKRM NK3R_HUMAN 107 14 -
IWIILAHKRM NK1R_RAT 54 14 -
IWIILAHKRM NK1R_MOUSE 54 14 -
MWIILAHKRM NK1R_HUMAN 54 14 -
MWIILAHKRM NK1R_CAVPO 54 14 -
IWIILAHRRM NK2R_HUMAN 55 14 -
IWIILAHERM NK2R_RAT 55 14 -
TWIILAHQRM NK2R_CAVPO 55 14 -
IWIILAHERM NK2R_MESAU 55 14 -
IWIILAHERM NK2R_MOUSE 55 14 -
IWIILAHQRM NK2R_BOVIN 55 14 -
IWIVLAHKRM NK4R_HUMAN 82 14 -
IWIILAHRRM NK2R_RABIT 55 14 -
MWIIIAHKRM NK1R_RANCA 55 14 -

Motif 3 width=11
Element Seqn Id St Int Rpt
FQNFFPITAVF NK3R_RAT 147 43 -
FQNFFPITAVF NK3R_HUMAN 160 43 -
FHNFFPIAALF NK1R_RAT 107 43 -
FHNFFPIAALF NK1R_MOUSE 107 43 -
FHNFFPIAAVF NK1R_HUMAN 107 43 -
FHNFFPIAAVF NK1R_CAVPO 107 43 -
FQNLFPITAMF NK2R_HUMAN 108 43 -
FQNLFPITAMF NK2R_RAT 108 43 -
FQNLFPITAMF NK2R_CAVPO 108 43 -
FQNLFPITAMF NK2R_MESAU 108 43 -
FQNLFPVTAMF NK2R_MOUSE 108 43 -
FQNLFPITAMF NK2R_BOVIN 108 43 -
FQNFFPITAVF NK4R_HUMAN 135 43 -
FQNLFPITAMF NK2R_RABIT 108 43 -
FHNFFPISAVF NK1R_RANCA 108 43 -

Motif 4 width=11
Element Seqn Id St Int Rpt
LKPRLSATATK NK3R_RAT 178 20 -
LKPRLSATATK NK3R_HUMAN 191 20 -
LQPRLSATATK NK1R_RAT 138 20 -
LQPRLSATATK NK1R_MOUSE 138 20 -
LQPRLSATATK NK1R_HUMAN 138 20 -
LQPRLSATATK NK1R_CAVPO 138 20 -
FQPRLSAPSTK NK2R_HUMAN 139 20 -
FQPRLSAPSTK NK2R_RAT 139 20 -
FQPRLSAPSTK NK2R_CAVPO 139 20 -
FQPRLSAPITK NK2R_MESAU 139 20 -
FQPRLSAPSTK NK2R_MOUSE 139 20 -
FQPRLSAPGTR NK2R_BOVIN 139 20 -
LKPRLSATATR NK4R_HUMAN 166 20 -
FQPRLSGPGTK NK2R_RABIT 139 20 -
LKPRLSATATK NK1R_RANCA 139 20 -

Motif 5 width=10
Element Seqn Id St Int Rpt
YHIIVIILVY NK3R_RAT 234 45 -
YHIIVIILVY NK3R_HUMAN 247 45 -
YHICVTVLIY NK1R_RAT 196 47 -
YHICVTVLIY NK1R_MOUSE 196 47 -
YHICVTVLIY NK1R_HUMAN 196 47 -
YHICVTVLIY NK1R_CAVPO 196 47 -
YHLVVIALIY NK2R_HUMAN 197 47 -
YHLVVFVLIY NK2R_RAT 197 47 -
YHLVVIVLIY NK2R_CAVPO 197 47 -
YHLVVFVLVY NK2R_MESAU 197 47 -
YHLVVFVLIY NK2R_MOUSE 197 47 -
YHLIVIALIY NK2R_BOVIN 197 47 -
YHMIVIVLVY NK4R_HUMAN 222 45 -
YHLTVIALIY NK2R_RABIT 197 47 -
YQVLVFCLIY NK1R_RANCA 198 48 -

Motif 6 width=14
Element Seqn Id St Int Rpt
TIVGITLWGGEIPG NK3R_RAT 255 11 -
TIVGITLWGGEIPG NK3R_HUMAN 268 11 -
TVVGITLWASEIPG NK1R_RAT 217 11 -
TVVGITLWASEIPG NK1R_MOUSE 217 11 -
TVVGITLWASEIPG NK1R_HUMAN 217 11 -
TVVGITLWASEIPG NK1R_CAVPO 217 11 -
SVIGLTLWRRAVPG NK2R_HUMAN 218 11 -
SVIGLTLWKRAVPR NK2R_RAT 218 11 -
SIIGLTLWRRAVPR NK2R_CAVPO 218 11 -
SVIGLTLWKRAVPR NK2R_MESAU 218 11 -
SVIGLTLWKRAVPR NK2R_MOUSE 218 11 -
SVIGLTLWRRSVPG NK2R_BOVIN 218 11 -
TIVGITLWGGEIPG NK4R_HUMAN 243 11 -
SVIGFKLWRRTVPG NK2R_RABIT 218 11 -
TFIGMTLWASEIPG NK1R_RANCA 219 11 -

Motif 7 width=12
Element Seqn Id St Int Rpt
WKYIQQVYLASF NK3R_RAT 318 49 -
WKYIQQVYLASF NK3R_HUMAN 331 49 -
KKFIQQVYLASM NK1R_RAT 280 49 -
KKFIQQVYLASM NK1R_MOUSE 280 49 -
KKFIQQVYLAIM NK1R_HUMAN 280 49 -
KKFIQQVYLAIM NK1R_CAVPO 280 49 -
HKFIQQVYLALF NK2R_HUMAN 282 50 -
HKFIQQVYLALF NK2R_RAT 282 50 -
HKFIQQVYLALF NK2R_CAVPO 282 50 -
RKFIQQVYLALF NK2R_MESAU 282 50 -
RKFIQQVYLALF NK2R_MOUSE 282 50 -
HKFIQQVYLALF NK2R_BOVIN 282 50 -
WKYIQQVYLASF NK4R_HUMAN 306 49 -
RKFIQQVYLVLF NK2R_RABIT 282 50 -
QKFYQQFYLAIM NK1R_RANCA 280 47 -

Motif 8 width=19
Element Seqn Id St Int Rpt
CCLNKRFRAGFKRAFRWCP NK3R_RAT 344 14 -
CCLNKRFRAGFKRAFRWCP NK3R_HUMAN 357 14 -
CCLNDRFRLGFKHAFRCCP NK1R_RAT 306 14 -
CCLNDRFRLGFKHAFRCCP NK1R_MOUSE 306 14 -
CCLNDRFRLGFKHAFRCCP NK1R_HUMAN 306 14 -
CCLNDRFRLGFKHAFRCCP NK1R_CAVPO 306 14 -
CCLNHRFRSGFRLAFRCCP NK2R_HUMAN 308 14 -
CCLNHRFRSGFRLAFRCCP NK2R_RAT 308 14 -
CCLNRRFRSGFRLAFRCCP NK2R_CAVPO 308 14 -
CCLNHRFRSGFRLAFRCCP NK2R_MESAU 308 14 -
CCLNHRFRSGFRLAFRCCP NK2R_MOUSE 308 14 -
CCLNHRFRSGFRLAFRCCP NK2R_BOVIN 308 14 -
CCLNKRFRAGFKRAFRWCP NK4R_HUMAN 332 14 -
CCLNHRFRSGFRLAFRCCP NK2R_RABIT 308 14 -
CCLNDRFRIGFKHVFRWCP NK1R_RANCA 306 14 -