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PR00242

Identifier
DOPAMINER  [View Relations]  [View Alignment]  
Accession
PR00242
No. of Motifs
5
Creation Date
14-JUL-1994  (UPDATE 28-JUL-1999)
Title
Dopamine receptor signature
Database References
PRINTS; PR90007 7TM; PR90006 GPCRCLAN; PR00237 GPCRRHODOPSN
PRINTS; PR00565 DOPAMINED1AR; PR00566 DOPAMINED1BR; PR00567 DOPAMINED2R
PRINTS; PR00568 DOPAMINED3R; PR00569 DOPAMINED4R
INTERPRO; IPR000929
GCRDB; GCR_0488
Literature References
1. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Fingerprinting G protein-coupled receptors.
PROTEIN ENG. 7(2) 195-203 (1994).
 
2. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
G protein-coupled receptor fingerprints.
7TM, VOLUME 2, EDS. G.VRIEND AND B.BYWATER (1993).
 
3. BIRNBAUMER, L.
G proteins in signal transduction.
ANNU.REV.PHARMACOL.TOXICOL. 30 675-705 (1990).
 
4. CASEY, P.J. AND GILMAN, A.G.
G protein involvement in receptor-effector coupling.
J.BIOL.CHEM. 263(6) 2577-2580 (1988).
 
5. ATTWOOD, T.K. AND FINDLAY, J.B.C. 
Design of a discriminating fingerprint for G protein-coupled receptors.
PROTEIN ENG. 6(2) 167-176 (1993).
 
6. GRANDY, D.K., MARCHIONNI, M.A., MAKAM, H., STOFKO, R.E., ALFANO, M.,
FROTHINGHAM, L., FISCHER, J.B., BURKE-HOWIE, K.J., BUNZOW, J.R.,
SERVER, A.C. AND CIVELLI, O.
Cloning of the cDNA and gene for a human D2 dopamine receptor. 
PROC.NATL.ACAD.SCI.U.S.A. 86 9762-9766 (1989).

Documentation
G protein-coupled receptors (GPCRs) constitute a vast protein family that 
encompasses a wide range of functions (including various autocrine, para-
crine and endocrine processes). They show considerable diversity at the 
sequence level, on the basis of which they can be separated into distinct 
groups. We use the term clan to describe the GPCRs, as they embrace a group
of families for which there are indications of evolutionary relationship, 
but between which there is no statistically significant similarity in 
sequence [1]. The currently known clan members include the rhodopsin-like 
GPCRs, the secretin-like GPCRs, the cAMP receptors, the fungal mating
pheromone receptors, and the metabotropic glutamate receptor family.
 
The rhodopsin-like GPCRs themselves represent a widespread protein family 
that includes hormone, neurotransmitter and light receptors, all of
which transduce extracellular signals through interaction with guanine
nucleotide-binding (G) proteins. Although their activating ligands vary 
widely in structure and character, the amino acid sequences of the 
receptors are very similar and are believed to adopt a common structural 
framework comprising 7 transmembrane (TM) helices [3-5]. 
 
Dopamine neurons in the vertebrate central nervous system are involved in
the initiation and execution of movement, the maintenance of emotional
stability, and the regulation of pituitary function [6]. Various human
neurological diseases (e.g., Parkinson disease and schizophrenia), are
believed to be manifestations of dopamine and dopamine receptor imbalance.
The receptors have been divided into several different subtypes, 
distinguished by their G protein coupling, ligand specificity, anatomical
distribution and physiological effects.
 
DOPAMINER is a 5-element fingerprint that provides a signature for the
dopamine receptors. The fingerprint was derived from an initial alignment
of 5 sequences: the motifs were drawn from conserved sections within either
loop or TM regions, focusing on those areas of the alignment that
characterise the dopamine receptors but distinguish them from the rest of
the rhodopsin-like superfamily. Two iterations on OWL23.2 were required
to reach convergence, at which point a true set comprising 17 sequences
was identified. Several partial matches were also found, most of which
are dopamine receptor fragments.
 
An update on SPTR37_9f identified a true set of 37 sequences, and 3
partial matches.
Summary Information
  37 codes involving  5 elements
1 codes involving 4 elements
0 codes involving 3 elements
2 codes involving 2 elements
Composite Feature Index
53737373737
410111
300000
210012
12345
True Positives
D1DR_CARAU    D1DR_FUGRU    D1DR_OREMO    D2D1_XENLA    
D2DR_BOVIN D2DR_CERAE D2DR_FUGRU D2DR_HUMAN
D2DR_MOUSE D3DR_CERAE D3DR_HUMAN D3DR_MOUSE
D3DR_RAT D4DR_HUMAN D4DR_MOUSE D5DR_FUGRU
DADR_DIDMA DADR_HUMAN DADR_PIG DADR_RAT
DADR_XENLA DBDR_HUMAN DBDR_RAT DBDR_XENLA
DCDR_XENLA O35838 O42315 O42316
O42317 O73810 O77680 Q13167
Q62610 Q98841 Q98842 Q98843
Q98844
True Positive Partials
Codes involving 4 elements
D4DR_RAT
Codes involving 2 elements
A2AA_PIG A2AR_CARAU
Sequence Titles
D1DR_CARAU  D(1) DOPAMINE RECEPTOR - CARASSIUS AURATUS (GOLDFISH). 
D1DR_FUGRU D(1)-LIKE DOPAMINE RECEPTOR - FUGU RUBRIPES (JAPANESE PUFFERFISH) (TAKIFUGU RUBRIPES).
D1DR_OREMO D(1)-LIKE DOPAMINE RECEPTOR - OREOCHROMIS MOSSAMBICUS (MOZAMBIQUE TILAPIA) (TILAPIA MOSSAMBICA).
D2D1_XENLA D(2) DOPAMINE RECEPTOR 1 - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
D2DR_BOVIN D(2) DOPAMINE RECEPTOR - BOS TAURUS (BOVINE).
D2DR_CERAE D(2) DOPAMINE RECEPTOR - CERCOPITHECUS AETHIOPS (GREEN MONKEY) (GRIVET).
D2DR_FUGRU D(2)-LIKE DOPAMINE RECEPTOR - FUGU RUBRIPES (JAPANESE PUFFERFISH) (TAKIFUGU RUBRIPES).
D2DR_HUMAN D(2) DOPAMINE RECEPTOR - HOMO SAPIENS (HUMAN).
D2DR_MOUSE D(2) DOPAMINE RECEPTOR - MUS MUSCULUS (MOUSE), AND RATTUS NORVEGICUS (RAT).
D3DR_CERAE D(3) DOPAMINE RECEPTOR - CERCOPITHECUS AETHIOPS (GREEN MONKEY) (GRIVET).
D3DR_HUMAN D(3) DOPAMINE RECEPTOR - HOMO SAPIENS (HUMAN).
D3DR_MOUSE D(3) DOPAMINE RECEPTOR - MUS MUSCULUS (MOUSE).
D3DR_RAT D(3) DOPAMINE RECEPTOR - RATTUS NORVEGICUS (RAT).
D4DR_HUMAN D(4) DOPAMINE RECEPTOR (D(2C) DOPAMINE RECEPTOR) - HOMO SAPIENS (HUMAN).
D4DR_MOUSE D(4) DOPAMINE RECEPTOR (D(2C) DOPAMINE RECEPTOR) - MUS MUSCULUS (MOUSE).
D5DR_FUGRU D(5)-LIKE DOPAMINE RECEPTOR - FUGU RUBRIPES (JAPANESE PUFFERFISH) (TAKIFUGU RUBRIPES).
DADR_DIDMA D(1A) DOPAMINE RECEPTOR - DIDELPHIS MARSUPIALIS VIRGINIANA (NORTH AMERICAN OPOSSUM).
DADR_HUMAN D(1A) DOPAMINE RECEPTOR - HOMO SAPIENS (HUMAN).
DADR_PIG D(1A) DOPAMINE RECEPTOR - SUS SCROFA (PIG).
DADR_RAT D(1A) DOPAMINE RECEPTOR - RATTUS NORVEGICUS (RAT).
DADR_XENLA D(1A) DOPAMINE RECEPTOR - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
DBDR_HUMAN D(1B) DOPAMINE RECEPTOR (D(5) DOPAMINE RECEPTOR) - HOMO SAPIENS (HUMAN).
DBDR_RAT D(1B) DOPAMINE RECEPTOR (D(5) DOPAMINE RECEPTOR) - RATTUS NORVEGICUS (RAT).
DBDR_XENLA D(1B) DOPAMINE RECEPTOR (D(5) DOPAMINE RECEPTOR) - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
DCDR_XENLA D(1C) DOPAMINE RECEPTOR - XENOPUS LAEVIS (AFRICAN CLAWED FROG).
O35838 DOPAMINE D4 RECEPTOR - MUS MUSCULUS (MOUSE).
O42315 D1A3 DOPAMINE RECEPTOR - CYPRINUS CARPIO (COMMON CARP).
O42316 D1A4 DOPAMINE RECEPTOR - CYPRINUS CARPIO (COMMON CARP).
O42317 D1B DOPAMINE RECEPTOR - CYPRINUS CARPIO (COMMON CARP).
O73810 D2 DOPAMINE RECEPTOR - MELEAGRIS GALLOPAVO (COMMON TURKEY).
O77680 D1 DOPAMINE RECEPTOR - MACACA MULATTA (RHESUS MACAQUE).
Q13167 DOPAMINE D3 RECEPTOR - HOMO SAPIENS (HUMAN).
Q62610 DOPAMINE D4 RECEPTOR - RATTUS NORVEGICUS (RAT).
Q98841 DOPAMINE D1A1 RECEPTOR - ANGUILLA ANGUILLA (EUROPEAN FRESHWATER EEL).
Q98842 DOPAMINE D1A2 RECEPTOR - ANGUILLA ANGUILLA (EUROPEAN FRESHWATER EEL).
Q98843 DOPAMINE D1B RECEPTOR - ANGUILLA ANGUILLA (EUROPEAN FRESHWATER EEL).
Q98844 DOPAMINE D1C RECEPTOR - ANGUILLA ANGUILLA (EUROPEAN FRESHWATER EEL).

D4DR_RAT D(4) DOPAMINE RECEPTOR (D(2C) DOPAMINE RECEPTOR) - RATTUS NORVEGICUS (RAT).

A2AA_PIG ALPHA-2A ADRENERGIC RECEPTOR (ALPHA-2A ADRENOCEPTOR) (ALPHA-2AAR) - SUS SCROFA (PIG).
A2AR_CARAU ALPHA-2 ADRENERGIC RECEPTOR (ALPHA-2 ADRENOCEPTOR) - CARASSIUS AURATUS (GOLDFISH).
Scan History
OWL23_2    2  25   NSINGLE    
OWL28_0 1 50 NSINGLE
SPTR37_9f 3 60 NSINGLE
Initial Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
CAAVIRFRHLR DADR_HUMAN 45 45 -
CAAIVRSRHLR DBDR_HUMAN 62 62 -
CMAVSREKALQ D2DR_BOVIN 56 56 -
CAAVLRERALQ D3DR_MOUSE 51 51 -
CVSVATERALQ D4DR_HUMAN 56 56 -

Motif 2 width=10
Element Seqn Id St Int Rpt
WKAVAEIAGF DADR_HUMAN 80 24 -
WKAVAEVAGY DBDR_HUMAN 97 24 -
WVVYLEVVGE D2DR_BOVIN 90 23 -
WVVYLEVTGG D3DR_MOUSE 85 23 -
LFVYSEVQGG D4DR_HUMAN 90 23 -

Motif 3 width=9
Element Seqn Id St Int Rpt
AISSPFRYE DADR_HUMAN 124 34 -
AISRPFRYK DBDR_HUMAN 141 34 -
AVAMPMLYN D2DR_BOVIN 135 35 -
AVVMPVHYQ D3DR_MOUSE 131 36 -
AVAVPLRYN D4DR_HUMAN 136 36 -

Motif 4 width=12
Element Seqn Id St Int Rpt
IDSNTFDVFVWF DADR_HUMAN 308 175 -
VSETTFDVFVWF DBDR_HUMAN 336 186 -
IPPVLYSAFTWL D2DR_BOVIN 404 260 -
VSPELYRATTWL D3DR_MOUSE 406 266 -
VPPRLVSAVTWL D4DR_HUMAN 345 200 -

Motif 5 width=15
Element Seqn Id St Int Rpt
AFNADFRKAFSTLLG DADR_HUMAN 332 12 -
AFNADFQKVFAQLLG DBDR_HUMAN 360 12 -
TFNIEFRKAFLKILH D2DR_BOVIN 429 13 -
TFNIEFRKAFLKILS D3DR_MOUSE 431 13 -
VFNAEFRNVFRKALR D4DR_HUMAN 370 13 -
Final Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
CAAVIRFRHLR DADR_XENLA 44 44 -
CAAVTKFRHLR Q98842 43 43 -
CAAVTKFRHLR D1DR_CARAU 46 46 -
CAAVTRFRHLR Q98841 43 43 -
CAAVIKFRHLR D1DR_OREMO 57 57 -
CAAVIRFRHLR DADR_DIDMA 44 44 -
CAAVIRFRHLR O77680 45 45 -
CAAVIRFRHLR DADR_PIG 45 45 -
CSAVLKFRHLR Q98843 65 65 -
CAAVIRFRHLR DADR_HUMAN 45 45 -
CAAVTKFRHLR O42315 43 43 -
CAAVIKFRHLR Q98844 47 47 -
CAAVTKFRHLR O42316 43 43 -
CLAVIKFRHLR DCDR_XENLA 50 50 -
CTAVMRFRHLR DBDR_XENLA 63 63 -
CVAVTKFRHLR D1DR_FUGRU 45 45 -
CATVVRFRHLR O42317 36 36 -
CAAVIKFRHLR D5DR_FUGRU 61 61 -
CAAVIRFRHLR DADR_RAT 44 44 -
CAAIVRSRHLR DBDR_RAT 60 60 -
CAAIVRSRHLR DBDR_HUMAN 62 62 -
CMAVSREKALQ D2DR_HUMAN 56 56 -
CMAVSREKALQ D2DR_CERAE 56 56 -
CMAVSREKALQ D2DR_BOVIN 56 56 -
CMAVSREKALQ D2DR_MOUSE 56 56 -
CAAVLRERALQ D3DR_MOUSE 51 51 -
CMAVLKERALQ Q13167 51 51 -
CMAVLKERALQ D3DR_HUMAN 51 51 -
CMAVLKERALQ D3DR_CERAE 51 51 -
CAAVLRERALQ D3DR_RAT 51 51 -
CMAVSREKALQ O73810 53 53 -
CMAVSREKALQ D2DR_FUGRU 54 54 -
CIAVSREKALQ D2D1_XENLA 50 50 -
CVSVASERTLQ O35838 53 53 -
CVSVASERTLQ D4DR_MOUSE 53 53 -
CVSVASERILQ Q62610 53 53 -
CVSVATERALQ D4DR_HUMAN 56 56 -

Motif 2 width=10
Element Seqn Id St Int Rpt
WKAVAEIAGF DADR_XENLA 79 24 -
WKAVTEVAGF Q98842 78 24 -
WKAVTEVAGF D1DR_CARAU 81 24 -
WKAATEIVGF Q98841 78 24 -
WRAVSEVAGV D1DR_OREMO 92 24 -
WKAVAEIAGF DADR_DIDMA 79 24 -
WKAVAEIAGF O77680 80 24 -
WKAVAEIAGF DADR_PIG 80 24 -
WKAVAEVAGY Q98843 100 24 -
WKAVAEIAGF DADR_HUMAN 80 24 -
WKAATEIVGF O42315 78 24 -
WKAVSEVAGY Q98844 82 24 -
WKAASEIVGF O42316 78 24 -
WKAVTEVAGF DCDR_XENLA 85 24 -
WKAVAEVAGH DBDR_XENLA 98 24 -
WKAATEIMGF D1DR_FUGRU 80 24 -
WKAVAEVAGF O42317 71 24 -
WRAVSEVAGV D5DR_FUGRU 96 24 -
WKAVAEIAGF DADR_RAT 79 24 -
WKAVAEVAGY DBDR_RAT 95 24 -
WKAVAEVAGY DBDR_HUMAN 97 24 -
WVVYLEVVGE D2DR_HUMAN 90 23 -
WVVYLEVVGE D2DR_CERAE 90 23 -
WVVYLEVVGE D2DR_BOVIN 90 23 -
WVVYLEVVGE D2DR_MOUSE 90 23 -
WVVYLEVTGG D3DR_MOUSE 85 23 -
WVVYLEVTGG Q13167 85 23 -
WVVYLEVTGG D3DR_HUMAN 85 23 -
WVVYLEVTGG D3DR_CERAE 85 23 -
WVVYLEVTGG D3DR_RAT 85 23 -
WVVYLEVVGE O73810 87 23 -
WVVYLEVVGE D2DR_FUGRU 88 23 -
WAVYMEVVGE D2D1_XENLA 84 23 -
LFVYSEVQGG O35838 87 23 -
LFVYSEVQGG D4DR_MOUSE 87 23 -
LFVYSEVQGG Q62610 87 23 -
LFVYSEVQGG D4DR_HUMAN 90 23 -

Motif 3 width=9
Element Seqn Id St Int Rpt
AISSPFRYE DADR_XENLA 123 34 -
AISSPFRYE Q98842 122 34 -
AISSPFRYE D1DR_CARAU 125 34 -
AISSPFRYE Q98841 122 34 -
AISSPFRYE D1DR_OREMO 136 34 -
AISSPFRYE DADR_DIDMA 123 34 -
AISSPFRYE O77680 124 34 -
AISSPFRYE DADR_PIG 124 34 -
AISSPFRYE Q98843 144 34 -
AISSPFRYE DADR_HUMAN 124 34 -
AISSPFRYE O42315 122 34 -
AIASPFRYE Q98844 126 34 -
AISSPFRYE O42316 122 34 -
AIASPFRYE DCDR_XENLA 129 34 -
AISSPFRYE DBDR_XENLA 142 34 -
AISSPFRYE D1DR_FUGRU 124 34 -
AISSPFRYE O42317 115 34 -
AISNPFRYE D5DR_FUGRU 140 34 -
AISSPFQYE DADR_RAT 123 34 -
AISRPFRYE DBDR_RAT 139 34 -
AISRPFRYK DBDR_HUMAN 141 34 -
AVAMPMLYN D2DR_HUMAN 135 35 -
AVAMPMLYN D2DR_CERAE 135 35 -
AVAMPMLYN D2DR_BOVIN 135 35 -
AVAMPMLYN D2DR_MOUSE 135 35 -
AVVMPVHYQ D3DR_MOUSE 131 36 -
AVVMPVHYQ Q13167 130 35 -
AVVMPVHYQ D3DR_HUMAN 131 36 -
AVVMPVHYQ D3DR_CERAE 131 36 -
AVVMPVHYQ D3DR_RAT 131 36 -
AAAMPMLYN O73810 132 35 -
AVAMPMLYN D2DR_FUGRU 133 35 -
AVAMPMLYN D2D1_XENLA 129 35 -
AVTVPLRYN O35838 133 36 -
AVTVPLRYN D4DR_MOUSE 133 36 -
AVTVPLRYN Q62610 133 36 -
AVAVPLRYN D4DR_HUMAN 136 36 -

Motif 4 width=12
Element Seqn Id St Int Rpt
ISSTTFDVFVWF DADR_XENLA 311 179 -
VSSTTFNVFVWF Q98842 307 176 -
ISPTTFDVFVWF D1DR_CARAU 306 172 -
VSSTTFDVFVWF Q98841 305 174 -
VSDTTFSVFVWF D1DR_OREMO 301 156 -
IDSITFDVFVWF DADR_DIDMA 311 179 -
IDSITFDVFVWF O77680 308 175 -
IDSITFDVFVWF DADR_PIG 308 175 -
VSDTTFDVFVWF Q98843 325 172 -
IDSNTFDVFVWF DADR_HUMAN 308 175 -
ISSTTFDVFVWF O42315 304 173 -
VSDSTFNIFVWF Q98844 300 165 -
ISSTTFDVFVWF O42316 300 169 -
VSETTFNIFVWF DCDR_XENLA 305 167 -
VSETTFDIFVWF DBDR_XENLA 322 171 -
ISSTTFDVFVWF D1DR_FUGRU 307 174 -
VTDTTFDVFVWF O42317 281 157 -
VSDTTFNIFVWF D5DR_FUGRU 311 162 -
IDSITFDVFVWF DADR_RAT 308 176 -
VSETTFDIFVWF DBDR_RAT 331 183 -
VSETTFDVFVWF DBDR_HUMAN 336 186 -
IPPVLYSAFTWL D2DR_HUMAN 403 259 -
IPPVLYSAFTWL D2DR_CERAE 403 259 -
IPPVLYSAFTWL D2DR_BOVIN 404 260 -
IPPVLYSAFTWL D2DR_MOUSE 404 260 -
VSPELYRATTWL D3DR_MOUSE 406 266 -
VSPELYSATTWL Q13167 359 220 -
VSPELYSATTWL D3DR_HUMAN 360 220 -
VSPELYSATTWL D3DR_CERAE 360 220 -
VSPELYRATTWL D3DR_RAT 406 266 -
IPPAMYSAFTWL O73810 396 255 -
VPAEMYNAFTWL D2DR_FUGRU 423 281 -
IPQALYSAFTWL D2D1_XENLA 402 264 -
VSPRLVSAVTWL O35838 345 203 -
VSPRLVSAVTWL D4DR_MOUSE 345 203 -
VSPRLVSAVTWL Q62610 345 203 -
VPPRLVSAVTWL D4DR_HUMAN 345 200 -

Motif 5 width=15
Element Seqn Id St Int Rpt
AFNADFRKAFSNLLG DADR_XENLA 335 12 -
AFNADFRKAFSILLG Q98842 331 12 -
AFNADFRRAFAILLG D1DR_CARAU 330 12 -
AFNADFRKAFSTLLG Q98841 329 12 -
AFNADFRKAFSTILG D1DR_OREMO 325 12 -
AFNADFRKAFSTLLG DADR_DIDMA 335 12 -
AFNADFRKAFSTLLG O77680 332 12 -
AFNADFRKAFSTLLG DADR_PIG 332 12 -
AFNADFRKAFASLLG Q98843 349 12 -
AFNADFRKAFSTLLG DADR_HUMAN 332 12 -
AFNADFRKAFSTLLG O42315 328 12 -
AFNADFRKAFSTILG Q98844 324 12 -
AFNAGFRKAFSILLG O42316 324 12 -
AFNADFRKAFTTILG DCDR_XENLA 329 12 -
AFNADFRKVFSSLLG DBDR_XENLA 346 12 -
AFNADFRKAFSILLG D1DR_FUGRU 331 12 -
AFNAEFRRAFSSLLR O42317 305 12 -
AFNADFRKAFTTILG D5DR_FUGRU 335 12 -
AFNADFQKAFSTLLG DADR_RAT 332 12 -
AFNADFRKVFAQLLG DBDR_RAT 355 12 -
AFNADFQKVFAQLLG DBDR_HUMAN 360 12 -
TFNIEFRKAFLKILH D2DR_HUMAN 428 13 -
TFNIEFRKAFLKILH D2DR_CERAE 428 13 -
TFNIEFRKAFLKILH D2DR_BOVIN 429 13 -
TFNIEFRKAFMKILH D2DR_MOUSE 429 13 -
TFNIEFRKAFLKILS D3DR_MOUSE 431 13 -
TFNIEFRKAFLKILS Q13167 384 13 -
TFNIEFRKAFLKILS D3DR_HUMAN 385 13 -
TFNIEFRKAFLKILS D3DR_CERAE 385 13 -
TFNVEFRKAFLKILS D3DR_RAT 431 13 -
TFNIEFRKAFMKILH O73810 421 13 -
TFNVEFRKAFIKILH D2DR_FUGRU 448 13 -
TFNVEFRKAFIKILH D2D1_XENLA 427 13 -
IFNAEFRSVFRKTLR O35838 370 13 -
IFNAEFRSVFRKTLR D4DR_MOUSE 370 13 -
IFNAEFRSVFRKTLR Q62610 370 13 -
VFNAEFRNVFRKALR D4DR_HUMAN 370 13 -