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PR00211

Identifier
GLUTELIN  [View Relations]  [View Alignment]  
Accession
PR00211
No. of Motifs
7
Creation Date
21-OCT-1992  (UPDATE 10-JUN-1999)
Title
Glutelin signature 
Database References

INTERPRO; IPR000480
Literature References
1. LEITE, A., DEFREITAS, F.A., YUNES, J.A. AND ARRUDA, P.
Nucleotide sequence of a cDNA clone encoding gamma-coixin from Coix
lacryma-jobi seeds.
PLANT PHYSIOL. 97(4) 1604-1605 (1991).
 
2. PRAT, S., CORTADAS, J., PUIGDOMENECH, P. AND PALAU, J.
Nucleic acid (cDNA) and amino-acid sequences of the maize endosperm protein
glutelin-2.
NUCLEIC ACIDS RES. 13(5) 1493-1504 (1985).

Documentation
Glutelins are major storage proteins that aggregate in protein bodies in 
the endosperm of maize [1,2]. They comprise the second largest protein 
fraction in maize endosperm [2] (zeins being the largest), and show 
sequence similarities to other cereal storage proteins, such as gliadins, 
glutenins, hordeins, etc.. Glutelins have a well-defined structure, 
including an N-terminal region containing varying numbers of repeats of 
the sequence PPPHVL [1]; a Gln rich region that can be separated into 2 
domains; and a Cys rich C-terminal domain that shows some regions of 
internal similarity. Glutelins have been shown [2] to exhibit structural 
similarity to other cereal storage proteins, including a beta-reverse 
turn region which forms a loose helix-like domain.
 
GLUTELIN is a 4-element fingerprint that provides a signature for the
glutelins. The fingerprint was derived from an initial alignment of 3
sequences: the motifs were drawn from conserved regions spanning virtually
the full alignment length. Two iterations on OWL18.0 were required to reach
convergence, at which point a true set comprising 4 sequences was 
identified.
 
An update on SPTR57.15_40.15f identified a true set of 14 sequences, and 2
partial matches.
Summary Information
   5 codes involving  7 elements
0 codes involving 6 elements
0 codes involving 5 elements
0 codes involving 4 elements
0 codes involving 3 elements
1 codes involving 2 elements
Composite Feature Index
75555555
60000000
50000000
40000000
30000000
20000000
1234567
True Positives
GLU2_MAIZE    Q00318        Q41295        Q41506        
ZEB2_MAIZE
True Positive Partials
Codes involving 2 elements
PRO2_ORYSA
Sequence Titles
GLU2_MAIZE  GLUTELIN 2 PRECURSOR (ZEIN-GAMMA) (27 KD ZEIN) (ALCOHOL-SOLUBLE REDUCED GLUTELIN) (ASG) (ZEIN ZC2) - ZEA MAYS (MAIZE). 
Q00318 22 KD GAMMA-COIXIN PRECURSOR - COIX LACHRYMA-JOBI (JOBS'TEARS).
Q41295 ENDOSPERM TISSUE PRECURSOR - SORGHUM BICOLOR MILO (SORGHUM).
Q41506 GAMMA-KAFIRIN PREPROTEIN PRECURSOR - SORGHUM VULGARE (SORGHUM).
ZEB2_MAIZE ZEIN-BETA PRECURSOR (ZEIN 2) (16 KD) (ZEIN ZC1) - ZEA MAYS (MAIZE).

PRO2_ORYSA 13 KD PROLAMIN PRECURSOR - ORYZA SATIVA (RICE).
Scan History
OWL18_0    2  100  NSINGLE    
OWL19_1 1 100 NSINGLE
OWL26_0 1 100 NSINGLE
SPTR37_9f 2 12 NSINGLE
Initial Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
VLLVALALLALAASATST GLU2_MAIZE 2 2 -
VLLVALALLALAASAAST SRGENSPMRN 2 2 -
VLLVALALLALTASATST CIXJGACOIX 2 2 -

Motif 2 width=21
Element Seqn Id St Int Rpt
PPPVHLPPPVHLPPPVHLPPP GLU2_MAIZE 30 10 -
PPPVHLPPPVHLPPPVHLPPP CIXJGACOIX 34 14 -
PPPVHLPPPVHLPPPVHLPPP SRGENSPMRN 34 14 -

Motif 3 width=17
Element Seqn Id St Int Rpt
CVEFLRHQCSPTATPYC GLU2_MAIZE 127 76 -
CIEFLRHQCSPAATPYC SRGENSPMRN 110 55 -
CIEFLRHQCSPAATPYC CIXJGACOIX 102 47 -

Motif 4 width=13
Element Seqn Id St Int Rpt
SLRQQCCQQLRQV GLU2_MAIZE 149 5 -
ALRQQCCHQLRQV CIXJGACOIX 124 5 -
ALRQQCCQQLRQV SRGENSPMRN 132 5 -

Motif 5 width=17
Element Seqn Id St Int Rpt
EPLHRYQAIFGVVLQSI SRGENSPMRN 145 0 -
EPQHRYQAIFGLVLQSI GLU2_MAIZE 162 0 -
EPLHRQQAIFGVVLQSI CIXJGACOIX 137 0 -

Motif 6 width=15
Element Seqn Id St Int Rpt
LMAAQIAQQLTVMCG CIXJGACOIX 164 10 -
LMAAQIAQQLTAMCG SRGENSPMRN 176 14 -
LLAAQIAQQLTAMCG GLU2_MAIZE 191 12 -

Motif 7 width=13
Element Seqn Id St Int Rpt
TSPCPCSAAAGGA CIXJGACOIX 184 5 -
PTPCPYAAAGGVP GLU2_MAIZE 209 3 -
PSPCASCSPFAGG SRGENSPMRN 196 5 -
Final Motifs
Motif 1  width=18
Element Seqn Id St Int Rpt
VLLVALALLALAASAAST Q41295 3 3 -
VLLVALALLALASAASTL Q41506 3 3 -
VLLVALALLALTASATST Q00318 3 3 -
VLLVALALLALAASATST GLU2_MAIZE 2 2 -
VLIVALALLALAASAASS ZEB2_MAIZE 3 3 -

Motif 2 width=21
Element Seqn Id St Int Rpt
PPPVHLPPPVHLPPPVHLPPP Q41295 35 14 -
PPPVHLPPPVHLPPPVHLPPP Q41506 34 13 -
PPPVHLPPPVHLPPPVHLPPP Q00318 35 14 -
PPPVHLPPPVHLPPPVHLPPP GLU2_MAIZE 30 10 -
TPPFHLPPPFYMPPPFYLPPQ ZEB2_MAIZE 29 8 -

Motif 3 width=17
Element Seqn Id St Int Rpt
CIEFLRHQCSPAATPYC Q41295 111 55 -
CIEFLRHQCSPAATPYC Q41506 110 55 -
CIEFLRHQCSPAATPYC Q00318 103 47 -
CVEFLRHQCSPTATPYC GLU2_MAIZE 127 76 -
CVEFLRHQCSPAATPYG ZEB2_MAIZE 86 36 -

Motif 4 width=13
Element Seqn Id St Int Rpt
ALRQQCCQQLRQV Q41295 133 5 -
ALRQQCCQQLRQV Q41506 132 5 -
ALRQQCCHQLRQV Q00318 125 5 -
SLRQQCCQQLRQV GLU2_MAIZE 149 5 -
ALQQQCCHQIRQV ZEB2_MAIZE 108 5 -

Motif 5 width=17
Element Seqn Id St Int Rpt
EPLHRYQAIFGVVLQSI Q41295 146 0 -
EPLHRYQAIFGVVLQSI Q41506 145 0 -
EPLHRQQAIFGVVLQSI Q00318 138 0 -
EPQHRYQAIFGLVLQSI GLU2_MAIZE 162 0 -
EPLHRYQATYGVVLQSF ZEB2_MAIZE 121 0 -

Motif 6 width=15
Element Seqn Id St Int Rpt
LMAAQIAQQLTAMCG Q41295 177 14 -
LMAAQIAQQLTAMCG Q41506 176 14 -
LMAAQIAQQLTVMCG Q00318 165 10 -
LLAAQIAQQLTAMCG GLU2_MAIZE 191 12 -
LMAAQVAQQLTAMCG ZEB2_MAIZE 149 11 -

Motif 7 width=13
Element Seqn Id St Int Rpt
PSPCASCSPFAGG Q41295 197 5 -
PSPCASCSPFAGG Q41506 196 5 -
TSPCPCSAAAGGA Q00318 185 5 -
PTPCPYAAAGGVP GLU2_MAIZE 209 3 -
PGPCPCNAAAGGV ZEB2_MAIZE 169 5 -