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PR00202

Identifier
ANNEXINVI  [View Relations]  [View Alignment]  
Accession
PR00202
No. of Motifs
5
Creation Date
28-OCT-1993  (UPDATE 06-OCT-2003)
Title
Annexin type VI signature
Database References
PRINTS; PR00196 ANNEXIN
INTERPRO; IPR002393
MIM; 114070
Literature References
1. BARTON, G.J., NEWMAN, R.H., FREEMONT, P.S. AND CRUMPTON, M.J.
Amino acid sequence analysis of the annexin super-gene family of proteins.
EUR.J.BIOCHEM. 198 749-760 (1991).
 
2. BRAUN, E.L., KANG, S., NELSON, M.A. AND NATVIG, D.O.
Identification of the first fungal annexin: analysis of annexin gene
duplications and implications for eukaryotic evolution.
J.MOL.EVOL. 47 531-543 (1998).
 
3. BENZ, J. AND HOFMANN, A.
Annexins: from structure to function.
BIOL.CHEM. 378 177-183 (1997).
 
4. GEISOW, M.J.
Annexins-forms without function but not without fun.
TRENDS BIOTECHNOL. 9 180-181 (1991).

Documentation
The annexins (or lipocortins) are a family of proteins that bind to
phospholipids in a calcium-dependent manner [1]. They are distributed
ubiquitously in different tissues and cell types of higher and lower
eukaryotes, including mammals, fish, birds, Drosophila melanogaster,
Xenopus laevis, Caenorhabtidis elegans, Dictyostelium discoideum and
Neurospora crassa [2,3]. The plant annexins are somewhat distinct from
those found in other taxa [3].
 
Several distinct annexin subtypes exist, each of which has an amino-acid
sequence consisting of an N-terminal 'arm' followed by 4 or 8 copies of a
conserved domain of 61 residues (only one of these residues, an arginine,
is conserved between all copies). The calcium-binding sites are found in
the repeated domains [4]. Individual repeats (sometimes referred to as
endonexin folds) consist of 5 alpha-helices wound into a right-handed
superhelix. The biological roles of some annexin subtypes is unclear; the
family has been linked with inhibition of phospholipase activity, exo-
cytosis and endoctyosis, signal transduction, organisation of the extra-
cellular matrix, resistance to reactive oxygen species and DNA replication
[2]. Type VI annexin may also play a part in the regulation of some calcium
channels, and its presence may cause arrest of cell growth, before the
DNA-replication stage, in cells growing at low serum concentrations. This
annexin class is unusual in containing 8 repeats of the conserved domain
rather than 4: hence, it is likely that the protein has arisen from a 
gene-duplication event.
 
ANNEXIN VI is a 5-element fingerprint that provides a signature for type VI 
annexins. The fingerprint was derived from an initial alignment of 4 
sequences: the motifs were drawn from conserved regions spanning the full 
alignment length, focusing on those areas that characterise type VI 
annexins but distinguish them from other closely related annexin subtypes - 
motif 1 lies at the N-terminus; motif 2 resides in the first repeat; motif
3 lies in the second repeat; and motifs 4 and 5 reside between the fourth
and fifth repeats. Two iterations on SPTR41_24f were required to reach
convergence, at which point a true set comprising 7 sequences was identified.
Summary Information
7 codes involving  5 elements
0 codes involving 4 elements
0 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
577777
400000
300000
200000
12345
True Positives
ANX6_CHICK    ANX6_HUMAN    ANX6_MOUSE    ANX6_RAT      
Q8BSS4 Q8CEX0 Q99JX6
Sequence Titles
ANX6_CHICK  Annexin A6 (Annexin VI) (Lipocortin VI) (P68) (P70) (Protein III) (Chromobindin 20) (67 kDa calelectrin) (Calphobindin-II) (CPB-II) - Gallus gallus (Chicken). 
ANX6_HUMAN Annexin A6 (Annexin VI) (Lipocortin VI) (P68) (P70) (Protein III) (Chromobindin 20) (67 kDa calelectrin) (Calphobindin-II) (CPB-II) - Homo sapiens (Human).
ANX6_MOUSE Annexin A6 (Annexin VI) (Lipocortin VI) (P68) (P70) (Protein III) (Chromobindin 20) (67 kDa calelectrin) (Calphobindin-II) (CPB-II) - Mus musculus (Mouse).
ANX6_RAT Annexin A6 (Annexin VI) (Lipocortin VI) (P68) (P70) (Protein III) (Chromobindin 20) (67 kDa calelectrin) (Calphobindin-II) (CPB-II) (Calcium-binding protein CATA 65/67) - Rattus norvegicus (Rat).
Q8BSS4 Annexin A6 - Mus musculus (Mouse).
Q8CEX0 Annexin A6 - Mus musculus (Mouse).
Q99JX6 Similar to annexin A6 - Mus musculus (Mouse).
Scan History
SPTR41_24f 2  150  NSINGLE    
Initial Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
KIAQGAMYRGS ANX6_MOUSE 2 2 -
KIAQGAMYRGS ANX6_RAT 2 2 -
KPAQGAKYRGS ANX6_HUMAN 2 2 -
MAPKGKVYRGS ANX6_CHICK 1 1 -

Motif 2 width=11
Element Seqn Id St Int Rpt
ICQSYKSLYGK ANX6_MOUSE 57 44 -
ICQSYKSLYGK ANX6_RAT 57 44 -
VCQSYKSLYGK ANX6_HUMAN 57 44 -
ICQAYKSQYGK ANX6_CHICK 56 44 -

Motif 3 width=14
Element Seqn Id St Int Rpt
NEQMHQLVAAYKDA ANX6_MOUSE 123 55 -
NEQIHQLVAAYKDA ANX6_RAT 123 55 -
NEQMHQLVAAYKDA ANX6_HUMAN 123 55 -
NQEIHDLVAAYKDA ANX6_CHICK 122 55 -

Motif 4 width=14
Element Seqn Id St Int Rpt
FFPEAAQVAYQMWE ANX6_MOUSE 330 193 -
FFPEAAQVAYQMWE ANX6_RAT 330 193 -
FFPEAAQVAYQMWE ANX6_HUMAN 330 193 -
FFPEAAQVAYRMWE ANX6_CHICK 329 193 -

Motif 5 width=16
Element Seqn Id St Int Rpt
SAVSRVELKGTVCAAN ANX6_MOUSE 345 1 -
SAVSRVELKGTVRAAN ANX6_RAT 345 1 -
SAVARVELKGTVRPAN ANX6_HUMAN 345 1 -
SAVAKVELRGTVQPAS ANX6_CHICK 344 1 -
Final Motifs
Motif 1  width=11
Element Seqn Id St Int Rpt
KIAQGAMYRGS ANX6_MOUSE 2 2 -
KIAQGAMYRGS Q99JX6 3 3 -
KIAQGAMYRGS ANX6_RAT 2 2 -
KIAQGAMYRGS Q8BSS4 3 3 -
KIAQGAMYRGS Q8CEX0 3 3 -
KPAQGAKYRGS ANX6_HUMAN 2 2 -
MAPKGKVYRGS ANX6_CHICK 1 1 -

Motif 2 width=11
Element Seqn Id St Int Rpt
ICQSYKSLYGK ANX6_MOUSE 57 44 -
ICQSYKSLYGK Q99JX6 58 44 -
ICQSYKSLYGK ANX6_RAT 57 44 -
ICQNYKSLYGK Q8BSS4 58 44 -
ICQNYKSLYGK Q8CEX0 58 44 -
VCQSYKSLYGK ANX6_HUMAN 57 44 -
ICQAYKSQYGK ANX6_CHICK 56 44 -

Motif 3 width=14
Element Seqn Id St Int Rpt
NEQMHQLVAAYKDA ANX6_MOUSE 123 55 -
NEQMHQLVAAYKDA Q99JX6 124 55 -
NEQIHQLVAAYKDA ANX6_RAT 123 55 -
NEQMHQLVAAYKDA Q8BSS4 124 55 -
NEQMHQLVAAYKDA Q8CEX0 124 55 -
NEQMHQLVAAYKDA ANX6_HUMAN 123 55 -
NQEIHDLVAAYKDA ANX6_CHICK 122 55 -

Motif 4 width=14
Element Seqn Id St Int Rpt
FFPEAAQVAYQMWE ANX6_MOUSE 330 193 -
FFPEAAQVAYQMWE Q99JX6 331 193 -
FFPEAAQVAYQMWE ANX6_RAT 330 193 -
FFPEAAQVAYQMWE Q8BSS4 331 193 -
FFPEAAQVAYQMWE Q8CEX0 331 193 -
FFPEAAQVAYQMWE ANX6_HUMAN 330 193 -
FFPEAAQVAYRMWE ANX6_CHICK 329 193 -

Motif 5 width=16
Element Seqn Id St Int Rpt
SAVSRVELKGTVCAAN ANX6_MOUSE 345 1 -
SAVSRVELKGTVCAAN Q99JX6 346 1 -
SAVSRVELKGTVRAAN ANX6_RAT 345 1 -
SAVSRVELKGTVCAAN Q8BSS4 346 1 -
SAVSRVELKGTVCAAN Q8CEX0 346 1 -
SAVARVELKGTVRPAN ANX6_HUMAN 345 1 -
SAVAKVELRGTVQPAS ANX6_CHICK 344 1 -