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PR00200

Identifier
ANNEXINIV  [View Relations]  [View Alignment]  
Accession
PR00200
No. of Motifs
4
Creation Date
28-OCT-1993  (UPDATE 06-OCT-2003)
Title
Annexin type IV signature
Database References
PRINTS; PR00196 ANNEXIN
INTERPRO; IPR002391
MIM; 106491
Literature References
1. BARTON, G.J., NEWMAN, R.H., FREEMONT, P.S. AND CRUMPTON, M.J.
Amino acid sequence analysis of the annexin super-gene family of proteins.
EUR.J.BIOCHEM. 198 749-760 (1991).
 
2. BRAUN, E.L., KANG, S., NELSON, M.A. AND NATVIG, D.O.
Identification of the first fungal annexin: analysis of annexin gene
duplications and implications for eukaryotic evolution.
J.MOL.EVOL. 47 531-543 (1998).
 
3. BENZ, J. AND HOFMANN, A.
Annexins: from structure to function.
BIOL.CHEM. 378 177-183 (1997).
 
4. GEISOW, M.J.
Annexins-forms without function but not without fun.
TRENDS BIOTECHNOL. 9 180-181 (1991).

Documentation
The annexins (or lipocortins) are a family of proteins that bind to
phospholipids in a calcium-dependent manner [1]. They are distributed
ubiquitously in different tissues and cell types of higher and lower
eukaryotes, including mammals, fish, birds, Drosophila melanogaster,
Xenopus laevis, Caenorhabtidis elegans, Dictyostelium discoideum and
Neurospora crassa [2,3]. The plant annexins are somewhat distinct from
those found in other taxa [3].
 
Several distinct annexin subtypes exist, each of which has an amino-acid
sequence consisting of an N-terminal 'arm' followed by 4 or 8 copies of a
conserved domain of 61 residues (only one of these residues, an arginine,
is conserved between all copies). The calcium-binding sites are found in
the repeated domains [4]. Individual repeats (sometimes referred to as
endonexin folds) consist of 5 alpha-helices wound into a right-handed
superhelix. The biological roles of some annexin subtypes is unclear; the
family has been linked with inhibition of phospholipase activity, exo-
cytosis and endoctyosis, signal transduction, organisation of the extra-
cellular matrix, resistance to reactive oxygen species and DNA replication
[2]. The type IV class has not been identified in all species known to
possess annexins, but are thought to be required for the budding of 
clathrin-coated pits.
 
ANNEXINIV is a 4-element fingerprint that provides a signature for type 
IV annexins. The fingerprint was derived from an initial alignment of 6
sequences: the motifs were drawn from conserved regions spanning the N-
terminal half of the alignment, focusing on those areas that characterise
type IV annexins but distinguish them from related annexin subtypes -
motifs 1-3 lie in the first annexin repeat; and motif 4 resides in the
second repeat. Two iterations on SPTR41_24f were required to reach
convergence, at which point a true set comprising 8 sequences was
identified. A single partial match was also found, Q90X16, a type IV
annexin from Xenopus laevis that failed to match motif 1.
Summary Information
   8 codes involving  4 elements
1 codes involving 3 elements
0 codes involving 2 elements
Composite Feature Index
48888
30111
20000
1234
True Positives
ANX4_BOVIN    ANX4_CANFA    ANX4_HUMAN    ANX4_MOUSE    
ANX4_PIG ANX4_RAT Q8BSL2 Q9R0V2
True Positive Partials
Codes involving 3 elements
Q90X16
Sequence Titles
ANX4_BOVIN  Annexin A4 (Annexin IV) (Lipocortin IV) (Endonexin I) (Chromobindin 4) (Protein II) (P32.5) (Placental anticoagulant protein II) (PAP-II) (PP4-X) (35-beta calcimedin) (Carbohydrate-binding protein P33/P41) (P33/41) - Bos taurus (Bovine). 
ANX4_CANFA Annexin A4 (Annexin IV) (Lipocortin IV) (36 kDa zymogen granule membrane associated protein) (ZAP36) - Canis familiaris (Dog).
ANX4_HUMAN Annexin A4 (Annexin IV) (Lipocortin IV) (Endonexin I) (Chromobindin 4) (Protein II) (P32.5) (Placental anticoagulant protein II) (PAP-II) (PP4-X) (35-beta calcimedin) (Carbohydrate-binding protein P33/P41) (P33/41) - Homo sapiens (Human).
ANX4_MOUSE Annexin A4 (Annexin IV) - Mus musculus (Mouse).
ANX4_PIG Annexin A4 (Annexin IV) (Lipocortin IV) (Endonexin I) (Chromobindin 4) (Protein II) (P32.5) (Placental anticoagulant protein I) (PAP-II) (PP4-X) (35-beta calcimedin) - Sus scrofa (Pig).
ANX4_RAT Annexin A4 (Annexin IV) (Lipocortin IV) (36 kDa zymogen granule membrane associated protein) (ZAP36) - Rattus norvegicus (Rat).
Q8BSL2 Annexin A4 - Mus musculus (Mouse).
Q9R0V2 Truncated annexin IV - Mus musculus (Mouse).

Q90X16 Annexin 4 - Xenopus laevis (African clawed frog).
Scan History
SPTR41_24f 2  150  NSINGLE    
Initial Motifs
Motif 1  width=10
Element Seqn Id St Int Rpt
GILAYRNTAQ ANX4_MOUSE 38 38 -
GVLACRNTAQ ANX4_RAT 38 38 -
SVLAYRSTAQ ANX4_PIG 38 38 -
SVLAYRNTAQ ANX4_HUMAN 38 38 -
NVLAYRSTAQ ANX4_BOVIN 38 38 -
SVLAPRNTSQ ANX4_CANFA 38 38 -

Motif 2 width=11
Element Seqn Id St Int Rpt
QEIRSAYKSTI ANX4_MOUSE 49 1 -
QEIRTAYKSTI ANX4_RAT 49 1 -
QEIRTAYKSTI ANX4_PIG 49 1 -
QEIRTAYKSTI ANX4_HUMAN 49 1 -
QEIRTAYKTTI ANX4_BOVIN 49 1 -
QEIRTAYKSTI ANX4_CANFA 49 1 -

Motif 3 width=16
Element Seqn Id St Int Rpt
QVILGLMTPTVLYDVQ ANX4_MOUSE 77 17 -
QVILGMMTPTVLYDVQ ANX4_RAT 77 17 -
QVILGMMTPTVLYDVQ ANX4_PIG 77 17 -
QVIVGMMTPTVLYDVQ ANX4_HUMAN 77 17 -
QVILGMMTPTVLYDVQ ANX4_BOVIN 77 17 -
RVIVGMITPTVLYDVQ ANX4_CANFA 77 17 -

Motif 4 width=15
Element Seqn Id St Int Rpt
EIRRINQTYQQQYGR ANX4_MOUSE 119 26 -
EIRRINQTYQQQYGR ANX4_RAT 119 26 -
EIRRINQTYQLQYGR ANX4_PIG 119 26 -
EIRRISQTYQQQYGR ANX4_HUMAN 119 26 -
EIRRINQTYQLQYGR ANX4_BOVIN 119 26 -
ELRCINQTYQLQYGR ANX4_CANFA 119 26 -
Final Motifs
Motif 1  width=10
Element Seqn Id St Int Rpt
GILAYRNTAQ ANX4_MOUSE 38 38 -
GILAYRNTAQ Q9R0V2 39 39 -
GILAYRNTAQ Q8BSL2 39 39 -
GVLACRNTAQ ANX4_RAT 38 38 -
SVLAYRSTAQ ANX4_PIG 38 38 -
SVLAYRNTAQ ANX4_HUMAN 38 38 -
NVLAYRSTAQ ANX4_BOVIN 38 38 -
SVLAPRNTSQ ANX4_CANFA 38 38 -

Motif 2 width=11
Element Seqn Id St Int Rpt
QEIRSAYKSTI ANX4_MOUSE 49 1 -
QEIRSAYKSTI Q9R0V2 50 1 -
QEIRSAYKSTI Q8BSL2 50 1 -
QEIRTAYKSTI ANX4_RAT 49 1 -
QEIRTAYKSTI ANX4_PIG 49 1 -
QEIRTAYKSTI ANX4_HUMAN 49 1 -
QEIRTAYKTTI ANX4_BOVIN 49 1 -
QEIRTAYKSTI ANX4_CANFA 49 1 -

Motif 3 width=16
Element Seqn Id St Int Rpt
QVILGLMTPTVLYDVQ ANX4_MOUSE 77 17 -
QVILGLMTPTVLYDVQ Q9R0V2 78 17 -
QVILGLMTPTVLYDVQ Q8BSL2 78 17 -
QVILGMMTPTVLYDVQ ANX4_RAT 77 17 -
QVILGMMTPTVLYDVQ ANX4_PIG 77 17 -
QVIVGMMTPTVLYDVQ ANX4_HUMAN 77 17 -
QVILGMMTPTVLYDVQ ANX4_BOVIN 77 17 -
RVIVGMITPTVLYDVQ ANX4_CANFA 77 17 -

Motif 4 width=15
Element Seqn Id St Int Rpt
EIRRINQTYQQQYGR ANX4_MOUSE 119 26 -
EIRRINQTYQQQYGR Q9R0V2 120 26 -
EIRRINQTYQQQYGR Q8BSL2 120 26 -
EIRRINQTYQQQYGR ANX4_RAT 119 26 -
EIRRINQTYQLQYGR ANX4_PIG 119 26 -
EIRRISQTYQQQYGR ANX4_HUMAN 119 26 -
EIRRINQTYQLQYGR ANX4_BOVIN 119 26 -
ELRCINQTYQLQYGR ANX4_CANFA 119 26 -